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Jauss Honors Biology 3 Spring Final Review 2011 Study all labs, notes, and activities from Second Semester!! Plants 1. Characteristics of all plants – study plant diversity sheet 2. What is alternation of generations? 3. From what did plants evolve? What to plants need to survive on land? 4. Four main groups - Bryophytes, (mosses), Ferns, Gymnosperms and Flowering Plants 5. Vascular systems - Xylem and phloem – what does each carry and how do they differ in monocots and dicots? 6. Compare mono and dicots - stems, roots, flower, leaves Good chart and color code to study on roots, stems, and leaves lab. 7. Function and operation of stomates 8. Leaf pigments – chlorophyll role 9. Photosynthesis - process, equation – see vocab, section reviews and chapter review, and simulation 10. Cell Respiration - process, equation –– see vocab, section reviews and chapter review, and simulation 11. Types of fermentation and examples of use 12. Flower notes and parts of flower (label), know female and male parts! 13. Allelopathy, tropisms, hormones and nastic movements, (going green) and ethnobotany 14. Roots, stems and leaves notes and lab – id pictures of each Genetic Technology 1. Restriction enzymes: How are they used? Why are they used? How is a sticky end different from a blunt end? Be able to make cuts on a DNA map (linear or circular) with one or a combination of enzymes and plot the cuts on a gel electrophoresis. 2. DNA fingerprinting: Match a DNA fingerprint at a crime scene to the suspect, solve forensics problems. Gel electrophoresis: What is it? What are the steps involved? What is the charge of DNA? How does DNA travel differently from the dyes? What is the function of the comb? How do fragments of various sizes travel through the gel? 3. DNA Sequencing: Steps to sequence DNA? What materials are required to sequence DNA? Difference and role of dideoxynucleotides? How many genes and base pairs are in the human genome? 4. PCR: What is the purpose of PCR? What are the steps of PCR? How does temperature change throughout the steps? Be able to determine the # of copies given # of cycles. Role of primers? 5. Genetic Engineering: pGLO experiment - what happened on each plate? Why? What was the role of the various steps of the experiment, like heat shock, transformation solution? etc. What genes are contained on the pGLO plasmid? Why is bacteria a good choice for transformation? Which plates were transformed? How do you know? How is the arabinose operon turned on and off? How does the GFP operon work? Study background and conclusions to pGLO lab 6. Gene Regulation How does prokaryotic regulation differ from eukaryotic regulation? How is Sanger method different from the modern method? Describe the operation of the lactose operon and the tryptophan operon. Why do they function differently? What are hox genes? What is the purpose of the TATA box? What are introns and exons? Which drop out? Why is mRNA edited when making proteins? Animals 1. Body plan lab – examples, tissue layers, what each tissue layer develops into, draw and label one type of body plan, deutero vs protostomes – how are they different and examples of each, purpose of coelem. 2. Embryology lab – be able to recognize different stages, be able to identify the blasula, blastopore, blastocoel, gastrula, zygote (# cells?) and layers of tissue. 3. Phyla of invertebrates – study notes from tour – most in matching format 4. Mollusca and Cephalopoda and squid facts (structure/function) – study notes and lab handout. 5. Classes of vertebrates - study notes from class tour 6. 3 types of reproduction among the mammal orders. Placental, egg laying and pouches 7. Which invert phylum are vertebrates most closely related to? Why is this? 8. Digestion (intra and extracellular), Respiration, Circulation (open and closed), excretion, nervous response, movement and support (types of skeleton), and reproduction (sexual vs. asexual and internal vs external fertilization) for Invertebrates and Vertebrates. 9. Temperature control in types of vertebrates.