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My Dinosaur® Now you too can have your very own pet dinosaur!!! By: Dylan Smith Ever wondered what it would be like to own your very own dinosaur? • Well thanks to some smart scientist and the use of DNA, we are now able to clone you a real living dinosaur! • The smart scientist were able to gather a source of DNA from a couple of extinct dinosaurs. • Don’t forget the surrogate mother! • With birds being the closet relative to a dinosaur our team of researches were able to use a Hawk as the surrogate mother for the cloning. Restriction Enzymes • The original DNA is isolated and cut by restriction enzymes in specific areas • This procedure uses staggered cuts Gel Electrophoresis • Gel Electrophoresis is used to view the DNA that was cut out, what size the DNA is, and what has been inserted or deleted form the original DNA cDNA • cDNA is complementary DNA • It can be made into cDNA libraries • The scientist use the cDNA libraries to determine which genes to use in the cloning process Labeled Probe • The Labeled probe is used to determine the amino acid code for the DNA sample • The DNA probe is used to hybridize the clones containing the right DNA Southern Blotting • This sequence is used to get the right DNA sequence needed for cloning DNA Sequencing • This uses the genes nucleotide sequence for the genetic structure needed • The DNA sequence allows scientist to have defined fragments of DNA, which is greatly needed in the cloning process Polymerase Chain Reaction • This allows the scientist to make more DNA quicker, since PCR does not need an mRNA template to make more DNA RFLP Analysis • This is the final checkpoint for the cloned gene • It can detect the product for a disease and allow scientist to discard the gene and keep the disease from spreading Get you’re my Dinosaur® Today!!!