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Wolbachia: master manipulators of invertebrate biology
Wolbachia: master manipulators of invertebrate biology

Unipro UGENE Manual
Unipro UGENE Manual

... You're going to use only basic UGENE features and don't want to waste Internet traffic You have limited disk space Use the NGS package, if: You're going to analyze ChIP-Seq data with the Cistrome pipeline Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched fr ...
Unipro UGENE Manual
Unipro UGENE Manual

Designing synthetic MLPA probes - MRC
Designing synthetic MLPA probes - MRC

... corresponding NG_sequence here: http://www.ncbi.nlm.nih.gov/refseq/rsg/browse/. N.b. NG_sequences also include intronic sequences. Nucleotide. The combination of LPO and RPO. DNA oligonucleotide which, when annealed to a complementary DNA sequence, can be used as starting point for extension by a po ...
SARIDAKI A. and K. BOURTZIS. Wolbachia
SARIDAKI A. and K. BOURTZIS. Wolbachia

... and maternally-inherited symbiont, has recently attracted a lot of attention. Beside insects, Wolbachia are found in association with a wide variety of other invertebrate species, including mites, scorpions, spiders, crustaceans, filarial nematodes. Several surveys have indicated that Wolbachia may ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad

... mixed (multiplexed) together but are detected simultaneously, either as bands on an agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description above, primer pairs also include a matched fluorescent probe for each primer set, which allows multiple PCR reactions to be ...
Wolbachia`s Role In Classical Speciation Theory
Wolbachia`s Role In Classical Speciation Theory

... or for lethality of hybrid males. Since such patterns (when caused by genetic factors) are usually supposed to play an important role in speciation, there has been strong motivation for investigating Wolbachia’s role in speciation processes of their hosts. In particular, Wolbachia can induce a matin ...
Control of DNA excision efficiency in Paramecium
Control of DNA excision efficiency in Paramecium

... micronuclei undergo meiosis, whereas the macronuclei degenerate. The fusion of two gametic nuclei produces a zygotic nucleus. This nucleus divides twice and the daughter nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed through chromos ...
Molecular mechanics of the interactions of spermine with DNA: DNA
Molecular mechanics of the interactions of spermine with DNA: DNA

... of conformations, however, and there are a variety of distances and positions between the primary and secondary amino groups. Energetics of Spermine/DNA Complexes. Numerical results for spemune/DNA complexes are summarized in Tables I-IV. Because energies for each DNA sequence will be different, abs ...
KingFisher Pure DNA Plant Kit
KingFisher Pure DNA Plant Kit

... dispensers, for example, the Thermo Scientific™ Multidrop™ Combi and/ or the Thermo Scientific™ Versette™. Thermo Scientific™ BindIt™ Software 3.2 can be used for running ready-made and optimized protocols for the Thermo Scientific™ KingFisher™ Pure Kits. It is also possible to transfer the develope ...
Wolbachia–host interactions: connecting phenotype to genotype
Wolbachia–host interactions: connecting phenotype to genotype

... of Wolbachia infection is rapidly expanding, our knowledge of the molecular mechanisms that mediate these outcomes is very rudimentary. A key step forward has been the recent sequencing of two complete Wolbachia genomes, that of the wMel strain that induces CI in D. melanogaster [27] and that of t ...
Squills (Scilla s.lat., Hyacinthaceae) in the flora of the Czech
Squills (Scilla s.lat., Hyacinthaceae) in the flora of the Czech

(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real
(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real

... agars.3,4 Isolates suspected to possibly represent salmonellae (ie, lactose non-fermenting colonies with or without hydrogen sulfide production) are submitted for additional screening or identification, depending on individual laboratory practices.5-7 These practices, although effective, are timecon ...
International Agency for Research on Cancer (IARC)
International Agency for Research on Cancer (IARC)

... diagnosis. In a cohort study in China, a significant elevation in risk for hepatocellular carcinoma was found among people with aflatoxin metabolites in the urine, after adjustment for hepatitis B surface antigen positivity. The elevation in risk was particularly high among those excreting aflatoxin ...
Horizontal gene transfer from flowering plants to Gnetum
Horizontal gene transfer from flowering plants to Gnetum

... base pairs inserted before the GTGCG motif that is typical of the start of group II introns (Tables 3 and 4 and Figs. 1 and 6). Angiosperm-type nad1 exons found in Gnetum share five nucleotide substitutions with angiosperms that are not found in other seed plants. They are also characterized by a fo ...
Phylogenetic studies on the bacterial phylum`Verrucomicrobia`
Phylogenetic studies on the bacterial phylum`Verrucomicrobia`

...  Phylogenetic studies were performed using polyphasic taxonomic approaches on eleven chemoheterotrophic bacteria which were isolated from various marine environments. The phylogenetic tree based on the neighbour-joining (NJ) generated comparisons of the 16S rRNA gene sequences revealed that these is ...
Lactobacillus paracasei
Lactobacillus paracasei

the taxonomic status of mimulus sookensis
the taxonomic status of mimulus sookensis

... the M. guttatus complex with which it can grow sympatrically [M. nasutus and M. guttatus], particularly M. nasutus with which it shares its breeding behavior, certain life history traits and a similar morphology. It should be noted also that M. platycalyx was found growing in Oregon within a mile of ...
Defining characteristics of Tn5 Transposase non
Defining characteristics of Tn5 Transposase non

... In this manuscript, we investigate the effect of Tn5 Tnp non-specific DNA binding on the initial steps of the transposition mechanism leading to ES localization. A previously described hyperactive variant of Tnp containing E54K, M56A and L372P mutations is used in these studies (11,19–22). In this c ...
Press release
Press release

... Many breeding programs involve a significant increase in the number of samples, which can be technically challenging for breeders if they are to analyse plants rapidly and precisely. The first step in the selection process consists of purifying the DNA, which needs to be purified for analysis or, in ...
Fingerprinting and Identification of Bacteria Present in UASB
Fingerprinting and Identification of Bacteria Present in UASB

... is dependent on the microbial composition of the UASB granules. The granules contain a consortium of bacteria, with a specific metabolic function for each group, contributing to the overall efficiency and stability of the bioreactor. The aim of this study was to fingerprint and identify the bacteria ...
FilmArray Blood Culture Panel Procedure in CLSI Template
FilmArray Blood Culture Panel Procedure in CLSI Template

... resolution DNA melting analysis on the PCR products and measures the fluorescence signal generated in each well (for more information see FilmArray Operator’s Manual). The FilmArray software then performs several analyses and assigns a final assay result. Analysis of melt curves. The FilmArray softw ...
Papaver(Papaver2)
Papaver(Papaver2)

... Considered by Petrovsky (1999, draft) as the major race. Distributed from the Jenisei area eastwards through Siberia, Alaska and Canada, however, in the text (1999) given as "West American". Petrovsky's map (1999) also totally excludes Greenland from the range of P. lapponicum. The problems with thi ...
Persistence and dispersion of Acinetobacter spp. in the urban water
Persistence and dispersion of Acinetobacter spp. in the urban water

... strains, the Subcommittee on the Taxonomy of Moraxella and Allied Bacteria suggested that the genus Acinetobacter should comprise only oxidase-negative strains. This division was supported by the use of several phenotypic tests, which have now been used for over three decades as the basis for inclus ...
2. Assessing the probative value of DNA evidence
2. Assessing the probative value of DNA evidence

... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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