Unipro UGENE Manual
... You're going to use only basic UGENE features and don't want to waste Internet traffic
You have limited disk space
Use the NGS package, if:
You're going to analyze ChIP-Seq data with the Cistrome pipeline
Explanation of the tip above: Some tools are embedded into UGENE as external. To be launched fr ...
Designing synthetic MLPA probes - MRC
... corresponding NG_sequence here: http://www.ncbi.nlm.nih.gov/refseq/rsg/browse/. N.b.
NG_sequences also include intronic sequences.
The combination of LPO and RPO.
DNA oligonucleotide which, when annealed to a complementary DNA sequence, can be
used as starting point for extension by a po ...
SARIDAKI A. and K. BOURTZIS. Wolbachia
... and maternally-inherited symbiont, has recently attracted a lot of attention. Beside insects,
Wolbachia are found in association with a wide variety of other invertebrate species, including
mites, scorpions, spiders, crustaceans, filarial nematodes. Several surveys have indicated that
Wolbachia may ...
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
... mixed (multiplexed) together but are detected simultaneously, either as bands on an
agarose gel or as a SYBR Green I dye signal in this real-time extension. In the description
above, primer pairs also include a matched fluorescent probe for each primer set, which
allows multiple PCR reactions to be ...
Wolbachia`s Role In Classical Speciation Theory
... or for lethality of hybrid males. Since such patterns (when caused by genetic
factors) are usually supposed to play an important role in speciation, there
has been strong motivation for investigating Wolbachia’s role in speciation
processes of their hosts. In particular, Wolbachia can induce a matin ...
Control of DNA excision efficiency in Paramecium
... micronuclei undergo meiosis, whereas the macronuclei
degenerate. The fusion of two gametic nuclei produces a
zygotic nucleus. This nucleus divides twice and the daughter
nuclei then differentiate into a micronucleus or a macronucleus. In the second case, the whole genome is processed
through chromos ...
Molecular mechanics of the interactions of spermine with DNA: DNA
... of conformations, however, and there are a variety of distances
and positions between the primary and secondary amino groups.
Energetics of Spermine/DNA Complexes. Numerical results for
spemune/DNA complexes are summarized in Tables I-IV.
Because energies for each DNA sequence will be different,
KingFisher Pure DNA Plant Kit
... dispensers, for example, the Thermo Scientific™ Multidrop™ Combi and/
or the Thermo Scientific™ Versette™. Thermo Scientific™ BindIt™
Software 3.2 can be used for running ready-made and optimized protocols
for the Thermo Scientific™ KingFisher™ Pure Kits. It is also possible
to transfer the develope ...
Wolbachia–host interactions: connecting phenotype to genotype
... of Wolbachia infection is rapidly expanding, our knowledge of the molecular mechanisms that mediate these
outcomes is very rudimentary. A key step forward has
been the recent sequencing of two complete Wolbachia
genomes, that of the wMel strain that induces CI in
D. melanogaster  and that of t ...
(Pan) Salmonella and Salmonella Serotype Typhi–Specific Real
... agars.3,4 Isolates suspected to possibly represent salmonellae
(ie, lactose non-fermenting colonies with or without hydrogen sulfide production) are submitted for additional screening or identification, depending on individual laboratory
practices.5-7 These practices, although effective, are timecon ...
International Agency for Research on Cancer (IARC)
... diagnosis. In a cohort study in China, a significant elevation in risk for hepatocellular
carcinoma was found among people with aflatoxin metabolites in the urine, after
adjustment for hepatitis B surface antigen positivity. The elevation in risk was particularly
high among those excreting aflatoxin ...
Horizontal gene transfer from flowering plants to Gnetum
... base pairs inserted before the GTGCG motif that is typical of the
start of group II introns (Tables 3 and 4 and Figs. 1 and 6).
Angiosperm-type nad1 exons found in Gnetum share five nucleotide substitutions with angiosperms that are not found in
other seed plants. They are also characterized by a fo ...
Phylogenetic studies on the bacterial phylum`Verrucomicrobia`
... Phylogenetic studies were performed using polyphasic taxonomic approaches on eleven chemoheterotrophic bacteria which were isolated from various marine environments. The phylogenetic tree
based on the neighbour-joining (NJ) generated comparisons of the 16S rRNA gene sequences revealed
that these is ...
the taxonomic status of mimulus sookensis
... the M. guttatus complex with which it can grow sympatrically [M. nasutus and M. guttatus],
particularly M. nasutus with which it shares its breeding behavior, certain life history traits and a
similar morphology. It should be noted also that M. platycalyx was found growing in Oregon within
a mile of ...
Defining characteristics of Tn5 Transposase non
... In this manuscript, we investigate the effect of Tn5 Tnp
non-specific DNA binding on the initial steps of the transposition mechanism leading to ES localization. A previously
described hyperactive variant of Tnp containing E54K,
M56A and L372P mutations is used in these studies
(11,19–22). In this c ...
... Many breeding programs involve a significant increase in the number of samples, which can
be technically challenging for breeders if they are to analyse plants rapidly and precisely.
The first step in the selection process consists of purifying the DNA, which needs to be
purified for analysis or, in ...
Fingerprinting and Identification of Bacteria Present in UASB
... is dependent on the microbial composition of the UASB granules. The granules contain a consortium of bacteria,
with a specific metabolic function for each group, contributing to the overall efficiency and stability of the
bioreactor. The aim of this study was to fingerprint and identify the bacteria ...
FilmArray Blood Culture Panel Procedure in CLSI Template
... resolution DNA melting analysis on the PCR products and measures the
fluorescence signal generated in each well (for more information see FilmArray
Operator’s Manual). The FilmArray software then performs several analyses and
assigns a final assay result.
Analysis of melt curves. The FilmArray softw ...
... Considered by Petrovsky (1999, draft) as the major race. Distributed from the Jenisei
area eastwards through Siberia, Alaska and Canada, however, in the text (1999) given
as "West American". Petrovsky's map (1999) also totally excludes Greenland from
the range of P. lapponicum. The problems with thi ...
Persistence and dispersion of Acinetobacter spp. in the urban water
... strains, the Subcommittee on the Taxonomy of Moraxella and Allied Bacteria suggested that the
genus Acinetobacter should comprise only oxidase-negative strains. This division was supported
by the use of several phenotypic tests, which have now been used for over three decades as the
basis for inclus ...
2. Assessing the probative value of DNA evidence
... The four Practitioner Guides are being written over a four-year period, with the final
Guide scheduled for publication in 2013. They are intended, when completed, to form
a coherent package, but each Guide is also designed to function as a stand-alone
publication addressing a specific topic or set o ...
DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).