2. Assessing the probative value of DNA evidence
... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
... The four Practitioner Guides are being written over a four-year period, with the final Guide scheduled for publication in 2013. They are intended, when completed, to form a coherent package, but each Guide is also designed to function as a stand-alone publication addressing a specific topic or set o ...
Synapsis-Mediated Fusion of Free DNA Ends Forms Inverted Dimer Plasmids in Yeast.
... range of DNA concentrations. This observation was reproduced in several similar experiments with these plasmids or with the closely related plasmids, pSK 136 and pSK142 (shown in Figure 6A). As will be seen below, the recovery of Ura+ Leu+ transformants is due to theformation of heterozygous inverte ...
... range of DNA concentrations. This observation was reproduced in several similar experiments with these plasmids or with the closely related plasmids, pSK 136 and pSK142 (shown in Figure 6A). As will be seen below, the recovery of Ura+ Leu+ transformants is due to theformation of heterozygous inverte ...
Graph-based Methods for the Design of DNA - TUBdok
... The purpose of this thesis is to provide a contribution to the field of finding reliable DNA sequences for the encoding of entities in mathematical problems. The developed methods make use of the nearest neighbor DNA thermodynamic model as a biological fundament. The modelling method uses graph theo ...
... The purpose of this thesis is to provide a contribution to the field of finding reliable DNA sequences for the encoding of entities in mathematical problems. The developed methods make use of the nearest neighbor DNA thermodynamic model as a biological fundament. The modelling method uses graph theo ...
Emended description of the order Chlamydiales, proposal of
... DNA-DNA reassociation, in particular, was established as a tool for distinguishing species (< 70 YO homology) and genera (< 20 YOhomology) (Amann et al., 1995; Schleifer & Stackebrandt, 1983; Wayne et al., 1987). According to these criteria, DNA-DNA reassociation studies of new chlamydial strains an ...
... DNA-DNA reassociation, in particular, was established as a tool for distinguishing species (< 70 YO homology) and genera (< 20 YOhomology) (Amann et al., 1995; Schleifer & Stackebrandt, 1983; Wayne et al., 1987). According to these criteria, DNA-DNA reassociation studies of new chlamydial strains an ...
IMGT-ONTOLOGY and IMGT databases, tools and Web
... to be used in the domain of immunogenetics and immunoinformatics to ensure accuracy, consistency and coherence in IMGT. This has been the basis of IMGT-ONTOLOGY (Giudicelli and Lefranc, 1999), the first ontology in the domain, which allows the management of the immunogenetics knowledge for human and ...
... to be used in the domain of immunogenetics and immunoinformatics to ensure accuracy, consistency and coherence in IMGT. This has been the basis of IMGT-ONTOLOGY (Giudicelli and Lefranc, 1999), the first ontology in the domain, which allows the management of the immunogenetics knowledge for human and ...
Presence of multiple group I introns closely 23S rRNAs of lichen-forming
... rRNA large subunit gene from a number of common phycobionts of the Trebouxia genus. This study is part of a global investigation focused on the phylogenetic relationships among lichen phycobionts based on comparisons of the plastid-encoded rRNA genes and the evolutionary history of their introns wit ...
... rRNA large subunit gene from a number of common phycobionts of the Trebouxia genus. This study is part of a global investigation focused on the phylogenetic relationships among lichen phycobionts based on comparisons of the plastid-encoded rRNA genes and the evolutionary history of their introns wit ...
Binding of Hoechst with nucleic acids using fluorescence spectroscopy
... on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5[5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimidazole. Hoechst 33342 contains additional ethyl gr ...
... on DNA and RNA can optimize the use of these compounds and their analogues as medical drugs. Hoechst dyes 33342 and 33258 are closely related bisbenzimides. Hoechst 33258 is 2’-(4-hydroxyphenyl)-5[5-(4-methylpiperazine-1-yl) benzimidazo-2-yl]-benzimidazole. Hoechst 33342 contains additional ethyl gr ...
Introduction - Stellenbosch University
... There is ~2m of DNA in every somatic human cell Each human in composed of about 1012 cells Thus every human contains 2×1012 m of DNA =2×109km of DNA Distance from the sun to Uranus = 2.8 ×109km Each single human contains enough DNA to stretch from the sun to Uranus Bioinformatics, Stellenbosch Unive ...
... There is ~2m of DNA in every somatic human cell Each human in composed of about 1012 cells Thus every human contains 2×1012 m of DNA =2×109km of DNA Distance from the sun to Uranus = 2.8 ×109km Each single human contains enough DNA to stretch from the sun to Uranus Bioinformatics, Stellenbosch Unive ...
Phytopathology
... fragments and may be applicable for rapid identification and detection of plant pathogens associated with plants (20,21,26). An array of species-specific oligonucleotide probes representing the various pathogens of potato, built on a solid support such as a nylon membrane or microscope slide, could ...
... fragments and may be applicable for rapid identification and detection of plant pathogens associated with plants (20,21,26). An array of species-specific oligonucleotide probes representing the various pathogens of potato, built on a solid support such as a nylon membrane or microscope slide, could ...
Bioinformatics Explained
... The NCBI BLAST web pages and the BLAST command line tool offer a number of different options which can be changed in order to obtain the best possible result. Changing these parameters can have a great impact on the search result. It is not the scope of this document to comment on all of the options ...
... The NCBI BLAST web pages and the BLAST command line tool offer a number of different options which can be changed in order to obtain the best possible result. Changing these parameters can have a great impact on the search result. It is not the scope of this document to comment on all of the options ...
Introduction to the BLAST Suite and BLASTN
... is updated, for example if the annotation is revised by the author, then it will be given a new version number, DD148865.2. Both versions will be kept. Rather than force you to know which version is the latest, GenBank lets you retrieve the latest version by just entering the accession number (DD148 ...
... is updated, for example if the annotation is revised by the author, then it will be given a new version number, DD148865.2. Both versions will be kept. Rather than force you to know which version is the latest, GenBank lets you retrieve the latest version by just entering the accession number (DD148 ...
2 SINGLE-MOLECULE DNA:PROTEIN INTERACTIONS - VU-dare
... limits of optical tweezers and what are the parameters that define these limits? In most cases, the performance of an optical tweezers instrument is limited by instrument drift and environmental noise. These factors can, to a large extent be suppressed by placing instruments in sound-isolated, tempe ...
... limits of optical tweezers and what are the parameters that define these limits? In most cases, the performance of an optical tweezers instrument is limited by instrument drift and environmental noise. These factors can, to a large extent be suppressed by placing instruments in sound-isolated, tempe ...
what is alignment? - UWI St. Augustine
... Significance of local sequence alignment •In global alignment, an attempt is made to align the entire sequences, as many characters as possible. • In local alignment, stretches of sequence with the highest density of matches are given the highest priority, •generating one or more islands of matches ...
... Significance of local sequence alignment •In global alignment, an attempt is made to align the entire sequences, as many characters as possible. • In local alignment, stretches of sequence with the highest density of matches are given the highest priority, •generating one or more islands of matches ...
Differential Enzyme Targeting As an Evolutionary Adaptation to
... RAGE (Mizobuchi and Frohman 1993) (with the exception of the domestic cat, the cDNA of which had previously been cloned [Lumb, Purdue, and Danpure 1994]). The amplified region included all the sequence from exon 1 and most or all of the 59 UTR (see fig. 3A). Analysis of the sequences revealed the pr ...
... RAGE (Mizobuchi and Frohman 1993) (with the exception of the domestic cat, the cDNA of which had previously been cloned [Lumb, Purdue, and Danpure 1994]). The amplified region included all the sequence from exon 1 and most or all of the 59 UTR (see fig. 3A). Analysis of the sequences revealed the pr ...
Ribotyping of Clostridium perfringens from industrially produced
... DNA analysis (ribotyping), a method which analyses restriction fragment length polymorphisms in the chromosomal genes that encode rRNA, has been shown to be useful for microbial species identification and subtyping. Methods and Results: The current study has used ribotyping to examine 111 Cl. perfri ...
... DNA analysis (ribotyping), a method which analyses restriction fragment length polymorphisms in the chromosomal genes that encode rRNA, has been shown to be useful for microbial species identification and subtyping. Methods and Results: The current study has used ribotyping to examine 111 Cl. perfri ...
Equilibrium Statistics of Channel-confined DNA
... Introduction Chapter 1. Introduction This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels wi ...
... Introduction Chapter 1. Introduction This thesis concerns the statistical description of a very long molecule (a polymer) that is confined to a channel which is much narrower than the size of the molecule. The primary motivation is recent experiments where DNA molecules are inserted into channels wi ...
Representation and High-Quality Annotation of
... Clustering of EST sequences is an algorithmic approach to reconstruct underlying mRNA structures from the fragmentary information of large-scale EST collections. The approach reduces redundancy and increases information content by grouping overlapping EST representing the same transcript into cluste ...
... Clustering of EST sequences is an algorithmic approach to reconstruct underlying mRNA structures from the fragmentary information of large-scale EST collections. The approach reduces redundancy and increases information content by grouping overlapping EST representing the same transcript into cluste ...
Reproductive isolation and introgression between sympatric
... number of studies have found higher levels of genetic differentiation inside than outside chromosomal rearrangements between recently diverged species (Michel et al. 2006; Noor et al. 2007; McGaugh et al. 2012; Barb et al. 2014; Lohse et al. 2015). Even in the absence of rearrangements, interspecifi ...
... number of studies have found higher levels of genetic differentiation inside than outside chromosomal rearrangements between recently diverged species (Michel et al. 2006; Noor et al. 2007; McGaugh et al. 2012; Barb et al. 2014; Lohse et al. 2015). Even in the absence of rearrangements, interspecifi ...
5 DNA Replication
... starting from one origin can traverse the entire chromosome in a reasonable amount of time. The large linear chromosomes in eukaryotic cells, however, contain far too much DNA to be replicated speedily from a single origin. Eukaryotic replication proceeds at a rate ranging from 500 to 5,000 nucleoti ...
... starting from one origin can traverse the entire chromosome in a reasonable amount of time. The large linear chromosomes in eukaryotic cells, however, contain far too much DNA to be replicated speedily from a single origin. Eukaryotic replication proceeds at a rate ranging from 500 to 5,000 nucleoti ...
Chromosomes Carrying Meiotic Avoidance Loci
... et al., 2004; Conner et al., 2008). By contrast, two independent dominant loci control apomixis in Hieracium praealtum. Genetic markers linked to these loci have been determined using a deletion mapping approach (Catanach et al., 2006). The roles of these two loci and their interactions with the sex ...
... et al., 2004; Conner et al., 2008). By contrast, two independent dominant loci control apomixis in Hieracium praealtum. Genetic markers linked to these loci have been determined using a deletion mapping approach (Catanach et al., 2006). The roles of these two loci and their interactions with the sex ...
Recombinant DNA technology and molecular cloning
... The first cloning experiments Hamilton Smith and co-workers demonstrated unequivocally that restriction endoncleases cleave a specific DNA sequence. Later, Daniel Nathans used restriction endonucleases to map the simian virus 40 (SV40) genome and to locate the origin of replication. These major brea ...
... The first cloning experiments Hamilton Smith and co-workers demonstrated unequivocally that restriction endoncleases cleave a specific DNA sequence. Later, Daniel Nathans used restriction endonucleases to map the simian virus 40 (SV40) genome and to locate the origin of replication. These major brea ...
Mechanistic Comparison of High-Fidelity and Error
... free energy associated with each step. For deciphering kinetic mechanisms, pre-steady-state analyses are superior to steadystate analyses in that the former can provide rate information for the interconversion of reaction intermediates. Accordingly, when discussing kinetic mechanisms, this review wi ...
... free energy associated with each step. For deciphering kinetic mechanisms, pre-steady-state analyses are superior to steadystate analyses in that the former can provide rate information for the interconversion of reaction intermediates. Accordingly, when discussing kinetic mechanisms, this review wi ...
`Candidatus Phytoplasma mali`, `Candidatus Phytoplasma pyri` and
... Phytoplasma’ species if its 16S rDNA sequence has <97?5 % similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequence similarity, description as different ‘Candidatus’ species is only recommended if there is an indicat ...
... Phytoplasma’ species if its 16S rDNA sequence has <97?5 % similarity to that of any previously described ‘Candidatus Phytoplasma’ species’. In cases where phytoplasmas share ¢97?5 % 16S rDNA sequence similarity, description as different ‘Candidatus’ species is only recommended if there is an indicat ...
Bosque, a software system for phylogenetic analysis
... Tree Projects and Tree Windows The current molecular phylogenetic analyses use nucleotides and amino-acids sequence data to infer the phylogeny of organisms. These analyses, therefore, begin with the integration of a set of sequences of interest and then by their respective alignment, which is the i ...
... Tree Projects and Tree Windows The current molecular phylogenetic analyses use nucleotides and amino-acids sequence data to infer the phylogeny of organisms. These analyses, therefore, begin with the integration of a set of sequences of interest and then by their respective alignment, which is the i ...