Alu electrophoresis PCR lab
... by dissolving a solute usually a solid material into 100 mL of a solution such as water, buffer, DMSO, etc. Some solutes are not easily dissolved in solvents so you may need to stir, heat, sonicate or vortex the solution until it dissolves. Other types of % solutions are Weight/Weight (W/W) and Volu ...
... by dissolving a solute usually a solid material into 100 mL of a solution such as water, buffer, DMSO, etc. Some solutes are not easily dissolved in solvents so you may need to stir, heat, sonicate or vortex the solution until it dissolves. Other types of % solutions are Weight/Weight (W/W) and Volu ...
Supplemental Tables
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
Supplemental Material
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
... 1993; VIEIRA et al. 1997). End sequences from 593 of these P1 clones that map to unique sites within the genome were generated to anchor the assembly onto the polytene chromosome map. In cases where a reference sequence of D. virilis was available for the in situ localized probe, position of the seq ...
Systematic and Applied Microbiology - digital
... genus Enterococcus. Since both strains produced ammonia from arginine, according to the Bergey´s scheme they should be classified as E. faecalis or E. faecium. Taking into account that they were tyramine producers, both were considered E. faecalis strains. In order to complete the identification of ...
... genus Enterococcus. Since both strains produced ammonia from arginine, according to the Bergey´s scheme they should be classified as E. faecalis or E. faecium. Taking into account that they were tyramine producers, both were considered E. faecalis strains. In order to complete the identification of ...
reactive_oxygen_species
... ROS Future Research: ROS signaling and homeostasis and their roles in aging is not currently known. The biology and biochemistry of ROS is currently being studied, but is hard because of the numerous cellular effects it has. The genetic description of the cellular and organism mechanisms of ROS tole ...
... ROS Future Research: ROS signaling and homeostasis and their roles in aging is not currently known. The biology and biochemistry of ROS is currently being studied, but is hard because of the numerous cellular effects it has. The genetic description of the cellular and organism mechanisms of ROS tole ...
PDF
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
... manipulation of paramagnetic beads using a gradient of magnetic field [14]. The two techniques have been developing head to head to achieve nowadays a remarkable level of sophistication. They have their own unique advantages but, largely, offer similar capabilities and require the same workflow. The ...
Intriguing evidence of translocations in Discus fish
... heteropicnotic regions that indicated the presence of sex chromatin (Figures 2a, b and c). The chromosomal behavior in some meiotic phases of S. aequifasciatus and S. haraldi was similar, but differences were detected among S. aequifasciatus/S. haraldi and S. discus. In diplotene spermatocytes and o ...
... heteropicnotic regions that indicated the presence of sex chromatin (Figures 2a, b and c). The chromosomal behavior in some meiotic phases of S. aequifasciatus and S. haraldi was similar, but differences were detected among S. aequifasciatus/S. haraldi and S. discus. In diplotene spermatocytes and o ...
Crystal structures of -[Ru(phen)2dppz]2+ 1 with oligonucleotides
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
... This self-complementary sequence crystallizes to give a symmetrical duplex with a stoichiometry of three cations of 1 per duplex, or 1.5 cations of 1 per decamer strand. All the nucleic acid strands are equivalent in the crystal lattice, with the packing shown in Figure 1a. The conformation of a sin ...
Directions for Use Uracil-DNA Glycosylase (UNG), Cod
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ...
... Will UNG remove uracil from both ss- and dsDNA with the same efficiency? No, UNG removes uracil from ss-DNA at a rate of 200% compared to dsDNA. Will UNG remove uracil from RNA? Will it work with short oligos (e.g. 25-mer)? UNG does not act on uracil on the ribose sugar backbone. It will work on ...
Incorporation of reporter molecule
... modi®ed dNTP combinations identi®ed and con®rmed with these two model template assay systems were then tested further in the natural DNA template assay with a pUC19-derived template (see Materials and Methods). Evaluation of modi®ed dNTP substrates First, the substrate properties of each modi®ed dNT ...
... modi®ed dNTP combinations identi®ed and con®rmed with these two model template assay systems were then tested further in the natural DNA template assay with a pUC19-derived template (see Materials and Methods). Evaluation of modi®ed dNTP substrates First, the substrate properties of each modi®ed dNT ...
the evolution of genomic base composition in bacteria
... Base composition. The genomic base compositions of 78 eubacterial species were obtained from the Codon Usage Database (Nakamura et al. 1997; http://www.kazusa.or.jp/ codon/), which provides codon usage and GC content information based on the protein-coding genes available in GenBank (Table 1, Fig. 1 ...
... Base composition. The genomic base compositions of 78 eubacterial species were obtained from the Codon Usage Database (Nakamura et al. 1997; http://www.kazusa.or.jp/ codon/), which provides codon usage and GC content information based on the protein-coding genes available in GenBank (Table 1, Fig. 1 ...
as PDF
... An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low levels of mutagenicity and toxicity compared ...
... An alternative dsDNA stain is SYBR Green I, produced by Invitrogen. Despite the fact that SYBR Green is more expensive, it is 25 times more sensitive than ethidium bromide (Jin et al., 1994). SYBR Safe, a variant of SYBR Green, has been shown to have low levels of mutagenicity and toxicity compared ...
Transfer of genetic material between the
... the nuclear genome of the two organelle genomes follows different paths. It is impossible to derive the origin and the specific sequence of events that resulted in the capture and control of all these chloroplast-localized functional proteins. However, the continual transfer of chloroplast sequences ...
... the nuclear genome of the two organelle genomes follows different paths. It is impossible to derive the origin and the specific sequence of events that resulted in the capture and control of all these chloroplast-localized functional proteins. However, the continual transfer of chloroplast sequences ...
Analysis and nucleotide sequence of an origin of DNA replication in
... competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly different from the reported protocol (Singer et al., 1985). 500ng of plasmid DNA proved to be saturating under these conditions, pWHI266 prepared from E. coil a ...
... competency is found in the late log phase of growth with about 6 × l0 s transformants per 500 ng of plasmid DNA. This procedure is slightly different from the reported protocol (Singer et al., 1985). 500ng of plasmid DNA proved to be saturating under these conditions, pWHI266 prepared from E. coil a ...
Mitochondrial DNA (mtDNA) Biogenesis: Visualization and Duel
... and Mitchison 2008). In addition, the labeling procedure for EdU does not involve the harsh acid or enzyme digests needed for BrdU epitope recovery, and therefore allows greater flexibility and more comprehensive results by comparing its incorporation with that of other intracellular markers (Cappel ...
... and Mitchison 2008). In addition, the labeling procedure for EdU does not involve the harsh acid or enzyme digests needed for BrdU epitope recovery, and therefore allows greater flexibility and more comprehensive results by comparing its incorporation with that of other intracellular markers (Cappel ...
DNA Base Sequence Homology in Rhizoctonia solani Kuihn: Inter
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
... (6), indicating genetic homogeneity among isolates within these groups. Hybridization between isolates of different AG was 30% or less (6,15). Ranges of DNA hybridization values varied for different AG, and lower levels of hybridization have confirmed lack of homogeneity among isolates within AG-1, ...
tutorial - ppuigbo
... introduced. Choosing an unsuitable genetic code may generate errors messages. ...
... introduced. Choosing an unsuitable genetic code may generate errors messages. ...
Phylogenetic analysis of members of the genus Rickettsia using the
... Nei, 1987). The data were also examined by using parsimony and maximum-likelihood methods (, and in ). A bootstrap analysis was performed to investigate the stability of the trees obtained. Bootstrap values were obtained for a consensus tree based on 100 randomly generate ...
... Nei, 1987). The data were also examined by using parsimony and maximum-likelihood methods (, and in ). A bootstrap analysis was performed to investigate the stability of the trees obtained. Bootstrap values were obtained for a consensus tree based on 100 randomly generate ...