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Evolution of Postzygotic Reproductive Isolation in a - Bio
Evolution of Postzygotic Reproductive Isolation in a - Bio

... mascula # Orchis pauciflora, and O. paucif lora # Orchis quadripunctata (see table 1 for taxon names and authors). To avoid errors in hybrid identification, we included only hybrid individuals for which the identities of the parental species and their F1 status had been verified through molecular an ...
PDF version - EpiGeneSys
PDF version - EpiGeneSys

... saturation, and thus chromatin fibre compaction. However, this method consumes a large amount of material, and thus is often not practical.(comment 7) In some circumstances, a thorough analysis of protein content may be necessary ? see (Huynh et al, 2005). This may be the case when working with unus ...
BIO450 Primer Design Tutorial
BIO450 Primer Design Tutorial

... So long as the secondary sites of the two primers are quite far apart or are on the same strand they will not lead to a competing amplification product. When a primer is complementary to a repeated sequence in the genome there is a much greater chance that a competing product will occur. Two critica ...
Does premature aging of the mtDNA mutator mouse prove that
Does premature aging of the mtDNA mutator mouse prove that

... shortened lifespan. The authors of the reports concluded that their data provided a ‘causative link between mtDNA mutations and aging phenotypes in mammals’ (Trifunovic et al., 2004) and that ‘mtDNA mutations … may be a central mechanism driving mammalian aging’ (Kujoth et al., 2005). Intact mitocho ...
Sequence comparison of aflR from different Aspergillus species
Sequence comparison of aflR from different Aspergillus species

... Aflatoxin contamination of foods and feeds is a world-wide agricultural problem. Aflatoxin production requires expression of the biosynthetic pathway regulatory gene, aflR, which encodes a Cys6 Zn2 -type DNA-binding protein. Homologs of aflR from Aspergillus nomius, bombycis, parasiticus, flavus, and pse ...
Article PDF
Article PDF

... only 1.7% of condensations lasting for more than 1 ns and only 0.1% of condensations lasting for more than 10 ns. We observed a single long-lived condensation event that lasted for more than 100 ns, which is elaborated upon below. These collective results suggest that though there may be a few highl ...
PDF
PDF

... in introns are likely to be under the influence of natural selection in plants in general. Selective constraint (also known as functional or evolutionary constraint) is defined here as the factor by which evolutionary divergence of a functional sequence is reduced, relative to a neutrally evolving s ...
The presence of two UvrB subunits in the UvrAB complex ensures
The presence of two UvrB subunits in the UvrAB complex ensures

... signi®cant average volume. Since these unwrapped complexes are expected to have lost the ATP molecule, we decided to analyse these complexes further by determining the volumes of UvrB±DNA complexes that were isolated by washing in the absence of ATP. As expected, no wrapped complexes were observed a ...
Symbiosis and the Origin of Species
Symbiosis and the Origin of Species

... fortuitously came upon CI in intraspeciÞc crosses among geographic races (some only miles apart) that showed complex incompatibility relationships. In fact, he found approximately 15 different “crossing types” within the C. pipiens complex (Laven, 1959). He viewed these crossing types as evidence th ...
Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles
Phylogenetic analysis of Sarcocystis spp. of mammals and reptiles

... The composition of the ``A'' branch con®rmed the recently described close relationship of heteroxenous genera Toxoplasma and Neospora with homoxenous Isospora spp. [10]. The lack of stability of this branch in the ssrRNA-based trees [11± 13] appears to be one of the major sources of polytomy generat ...
Rolling circle transcription on smallest size double stranded DNA
Rolling circle transcription on smallest size double stranded DNA

... manner at the corners so that each vertex is structurally identical to the two other. This flattening of the normally spread and floppy structure of the branched junction introduce an internal stress in the motif greatly increasing stiffness (Liu, et al. 2004).The tensegrity motif stands out as the ...
Diversity of DNA methyltransferases that recognize asymmetric
Diversity of DNA methyltransferases that recognize asymmetric

... hemimethylated DNA, and it therefore needs only a single methylation event to restore the fully methylated state. This is in consistent with the fact that most of the DNA MTases studied exist as monomers in solution. Multiple lines of evidence suggest that some DNA MTases function as dimers. Further ...
Analysis of DNA transcription termination sequences of gene coding
Analysis of DNA transcription termination sequences of gene coding

... assumed that the main role could play Rho-independent termination, which is related to presence of palindromic sequences, which leads to formation of the hairpin structure and to dissociation of the ternary elongation complex (TEC). In this work, DNA sequences located after phaC1 gene belonging to n ...
A semantic taxonomy for diversity measures
A semantic taxonomy for diversity measures

... effects on ecosystem functioning. Testing the consequences of diversity on ecosystem processes will require measures to be available based on a rigorous conceptualization of their very meaning. In the last decades, literally dozens of measures of diversity have been proposed. However, rather than us ...
Two groups of human herpesvirus 6 identified by sequence
Two groups of human herpesvirus 6 identified by sequence

... (Martin et al., 1991; Lindquester & Pellett, 1991). In this study we investigated the molecular basis for differences between HH¥-6 strain groups by PCR and sequence analysis of 15 variants, including laboratory reference strains from both groups. We also studied, by PCR, strains in two potential pa ...
Variation in the size of the oqhcontaining linear
Variation in the size of the oqhcontaining linear

... B. burgdorferi was statistically smaller than the corresponding DNA molecules in the other species. However, the size differences are not great enough to be of diagnostic value (with the possible exception of differentiating B. burgdorferi isolates from B. garinii and group VS461 isolates on the bas ...
Nested PCR Assays for Detection of Monilinia fructicola in Stone
Nested PCR Assays for Detection of Monilinia fructicola in Stone

... species. Furthermore, morphological characteristics of the anamorphs are also similar among some Botryosphaeria species and can be strongly influenced by the ...
Accepted Version - CSIRO Research Publications Repository
Accepted Version - CSIRO Research Publications Repository

... A comparison of DNA methylation statuses between parental lines and their progenies generated from single seed descent over 30 generations showed that larger regions of DNA methylation were stable and changes of DNA methylation accumulated through generations [59,60]. The rate of spontaneous changes ...
Unit F212 - Molecules, biodiversity, food and health - Medium
Unit F212 - Molecules, biodiversity, food and health - Medium

... concerned, it would have been better if the candidate had clearly stated that 0.16326532 = 0.0266555 (as 0.1632653 does not equal 0.0266555). ...
Demarcation of coding and non-coding regions of DNA using linear
Demarcation of coding and non-coding regions of DNA using linear

... Deoxyribonucleic Acid (DNA) strand carries genetic information in the cell. A strand of DNA consists of nitrogenous molecules called nucleotides. Nucleotides triplets, or the codons, code for amino acids. There are two distinct regions in DNA, the gene and the intergenic DNA, or the junk DNA. Two re ...
Preparation of PCR Products for DNA Sequencing
Preparation of PCR Products for DNA Sequencing

... The polymerase chain reaction (PCR) is widely used to prepare product for DNA sequencing. High specificity and good yield are considered prerequisites to the generation of high-quality DNA sequence data directly from PCR product. However, despite measures taken to standardize PCR set-up and cycling ...
Horizontal transfer of non-LTR retrotransposons: artifact or rare event
Horizontal transfer of non-LTR retrotransposons: artifact or rare event

... 2008). For example, HT of CR1B elements was identified along with the analysis of non-LTR retrotransposons diversity in Maculinea genomes. Bioinformatic approaches can greatly expand opportunities to observe the distribution and evolutionary history of non-LTR retrotransposons in various eukaryotic ...
XSL Formatter - H:\XML
XSL Formatter - H:\XML

... What defines a set? Environmental sample, population, phylogenetic, and mutation sets all contain a group of sequences that spans the same gene or region of the genome. Environmental samples are derived from a group of unclassified or unknown organisms. A population set contains sequences from diffe ...
Structure of DIG
Structure of DIG

... DIG-dUTP is used as a dTTP analog, gets incorporated by DNA polymerase in place of T bases DIG ...
Matthew Kwong - GEP Community Server
Matthew Kwong - GEP Community Server

... three for each isoform, and Genscan predicts seven exons for the feature that aligns with the Blastx result (feature 1). Thus, there is a high possibility that contig 10 contains only part of the ey gene. Further comparison between the genome browser and transcript details indicate that it likely co ...
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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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