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Copenhagen1 - Buffalo Ontology Site
Copenhagen1 - Buffalo Ontology Site

... Problem of vagueness solved by recognizing that our categories apply to reality in such a way as to respect an ...
Phenotypic and Genotypic Characterization of Rhizobium species
Phenotypic and Genotypic Characterization of Rhizobium species

... which turn the dye from green to blue while, fast growing Rhizobum species are acidic in nature turns the media yellow (Somasegaran and Hoben,1985).All the 06 isolates grew faster on YMA plate containing bromothymol blue and showed an acidic reaction turning the medium from green to yellow in color. ...
Assessing the Homogeneity of Plasmid DNA: An Important
Assessing the Homogeneity of Plasmid DNA: An Important

... Plasmids are extra-chromosomal, double-stranded DNA molecules that may exist in several forms differing in topology and size. However, as plasmid DNA is being used as vectors for therapeutic genes, the development of good analytical processes to assess both purity and heterogeneity is of great impor ...
Direct Sequence Analysis of the 14q+ and 18q
Direct Sequence Analysis of the 14q+ and 18q

... PPROXIMATELY 85% of follicular lymphomas and Enzymatic amplification. DNA was extracted as previously 30% of diffuse large cell lymphomas have been demondescribedI4from 11 patients with histologically proven centroblastic/ strated to carry the t( 14;18)(q32.3;q21.3) chromosomal centrocytic follicula ...
Polymerase chain reaction (PCR) The polymerase chain reaction
Polymerase chain reaction (PCR) The polymerase chain reaction

... amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. Developed in 1983 by Kary Mullis, PCR is now a common and often indispensable technique used in medical and biological research labs for a v ...
DNA Structure Changes Coupled to Protein Binding
DNA Structure Changes Coupled to Protein Binding

... interpretation of cyclization data, this method has produced results in good agreement with other experimental techniques. Scanning probe microscopy (SPM), which includes scanning tunnel microscopy (STM) and atomic force microscopy (AFM), is a relatively new method that has gained increasing popular ...
Discovery and classification of ecological diversity in the
Discovery and classification of ecological diversity in the

... Survey of sequence divergence within and between bacterial taxa. We tested the hypothesis that sequence similarity clusters based on housekeeping protein genes should correspond to ecologically distinct groups of bacteria. Since species and subspecies are generally considered to be ecologically dist ...
Using a Single-Nucleotide Polymorphism to Predict
Using a Single-Nucleotide Polymorphism to Predict

... recognition is mediated by specialized taste cells that communicate with several brain regions through direct connections to sensory neurons. Taste perception is a two-step process. First, a taste molecule binds to a specific receptor on the surface of a taste cell. Then, the taste cell generates a ...
A Mathematical Formulation of DNA Computation
A Mathematical Formulation of DNA Computation

... T , and vice versa. DNA computation involves to use singlestranded DNA segments to code the problem, let the singlestranded DNA segments react in test tubes or substrate surfaces, and then to find DNA binding strands and interpret the results by applying bio-molecular techniques. This process can be ...
The polymerase chain reaction
The polymerase chain reaction

... template molecules after 35-40 cycles (even with poor efficiency) • The molar weight of this fragment (taking 330 daltons per nucleotide and 660 Da for an average base pair) is 99 000 Da • There are 6x1023 molecules (Avogadro number) in 99 000 g of the fragment • So 0.2 g (2x10-7 g) fragment has 9. ...
Development of an internally controlled real-time PCR
Development of an internally controlled real-time PCR

... respiratory samples obtained from eight patients (c. 30% (8 ⁄ 27) of the annual reported cases in The Netherlands) [3]. One of these samples had been shown previously to be PCR-positive with a different primer set [8,11], and six cases were confirmed serologically (the two remaining cases showed inc ...
マニュアル Megaprime DNA Labelling System
マニュアル Megaprime DNA Labelling System

... 10 minutes incubation at 37°C. This rapid labelling is achieved by the use of nonamer primers rather than the conventional hexamers (Figure 1). Nonamers allow for more efficient priming from the template DNA at 37°C, resulting in fast and efficient labelling of the DNA. A new alternative protocol ha ...
Structural basis for the inhibition of human alkyladenine Please share
Structural basis for the inhibition of human alkyladenine Please share

... from the expression vector at the N terminus of AAG. For fulllength AAG, this cleavage site results in a GPHM sequence prior to residue 1, and for the truncated form of AAG, this cleavage site results in the addition of residues Gly-80, Pro-81, His-82, and Met-83 prior to residue 84. We refer to thi ...
DNA SEQUENCING (using a Li
DNA SEQUENCING (using a Li

... methods, and their derivations. Both methods were first described in 1977. The first method (Maxam and Gilbert 1977) is based on specific chemical degradation of the DNA. The DNA is first end-labeled using 35s or 33P, followed by separation of the two strands on a gel. Four aliquots of the desired s ...
Introduc8on` Materials`and`methods` Conclusions` Results
Introduc8on` Materials`and`methods` Conclusions` Results

... •  Sensi3vity"variable"depending"on"prevalence," species,"and"concentra3on"method" •  50890%"sensi3vity"" •  Underes3mates"polyparasi3sm" •  qPCR"is"rapid,"quan3ta3ve,"and"high8throughput"" species8specific"method" •  GI"parasites"may"disrupt"normal"intes3nal"microbiota" •  Decreased"biodiversity"is" ...
Molecular Diagnostics 21
Molecular Diagnostics 21

DNA cloning
DNA cloning

An rpoB signature sequence provides unique resolution for the
An rpoB signature sequence provides unique resolution for the

... MEGA4 (Tamura et al., 2007). ML trees were computed with Phyml v.2.4.1 (Guindon & Gascuel, 2003) and the HKY (Hasegawa– Kishino–Yano) model of nucleotide substitution (Hasegawa et al., 1985) using one category of substitution rate. For each method, the robustness of each branch was estimated by non- ...
Exogenous selection rather than cytonuclear incompatibilities
Exogenous selection rather than cytonuclear incompatibilities

Recombinant DNA
Recombinant DNA

... If the rDNA sequences encode a gene that is expressed, then the presence of RNA and/or protein products of the recombinant gene can be detected, typically using RT­PCR or western hybridization methods.[8] Gross phenotypic changes are not the norm, unless the recombinant gene has been chosen and modi ...
Slide 1
Slide 1

Molecular cloning and evolutionary analysis of captive forest musk
Molecular cloning and evolutionary analysis of captive forest musk

Evolutionary History of Free-Swimming and
Evolutionary History of Free-Swimming and

... as for locomotion. The points at issue are which of these lifestyles represents the primitive condition for urochordates, and which lifestyle was possessed by the ancestor of all chordates. Haeckel (1868) extended his recapitulation theory to this case and proposed that the first chordates were free ...
as a PDF
as a PDF

... of Arabidopsis to assess the degree of isolation asymmetry at different postmating life stages. We found that pollen from Arabidopsis arenosa will usually fertilize ovules from Arabidopsis lyrata; the reverse receptivity being less complete. Maternal A. lyrata parents set more F1 hybrid seed, but ge ...
Trawling DNA Databases For Partial Matches: What Is The FBI
Trawling DNA Databases For Partial Matches: What Is The FBI

... and federal databases as combined in the National DNA Index System (NDIS) hold over seven million short tandem repeat (STR) profiles from convicted offenders as well as a growing number of people who were merely arrested or detained.3 When investigators recover a DNA sample from the scene of a crime ...
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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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