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BioinformaIcs Journal Club
BioinformaIcs Journal Club

Presence of a mitochondrial-type 70
Presence of a mitochondrial-type 70

... mainly on ribosomal RNA, show that three amitochondriate protist lineages, diplomonads, microsporidia, and trichomonads, emerge consistently at the base of the eukaryotic tree before groups having mitochondria. This suggests that these groups could have diverged before the mitochondrial endosymbiosi ...
Punctuated Equilibria: The Tempo and Mode of Evolution
Punctuated Equilibria: The Tempo and Mode of Evolution

... Of the few stronger cases, we concentrate on Gingerich's data for Hyopsodus and argue that it provides an excellent example of species selection under our model. We then review the data of several studies that have supported our model since we published it five years ago. The record of human evoluti ...
C.W. Cunningham 2004
C.W. Cunningham 2004

... but the dynamics and frequency of masculinization are not well understood (Hoeh et al. 1997; Saavedra et al. 1997; Ladoukakis and Zouros 2001). In this study, we focus on the ‘‘standard’’ M-mtDNA, which is easily distinguished from F-mtDNA at the DNA sequence level. For the remainder of this paper, ...
A simplified subtractive hybridization protocol used to isolate DNA
A simplified subtractive hybridization protocol used to isolate DNA

... et al. (1985) was used to isolate DNA sequences specific to Xylella fastidiosa isolated from diseased citrus plants. Xy. fastidiosa is a unique species in the genus and consists of several pathotypes causing diseases in economically important plants, including alfalfa, almond, grapevine, peach and p ...
RECOMBINANT-DNA METHODOLOGY
RECOMBINANT-DNA METHODOLOGY

... genetic diseases, the mutation does not conveniently occur right at a restriction site. However, in many cases, it just happens that the mutation that’s being diagnosed is associated with another, nearby mutation (polymorphism) that does alter some endonuclease cleavage site. This second site is clo ...
Troubleshooting Guide for DNA Electrophoresis
Troubleshooting Guide for DNA Electrophoresis

Genetic Technology - McGraw Hill Higher Education
Genetic Technology - McGraw Hill Higher Education

... manufacture short pieces of DNA of any sequence it is programmed to produce. The DNA synthesizer cannot easily make entire genes, but it can make small fragments that can act as primers to DNA replication. If one primer is made for each end of the region of interest, they act to bracket the region t ...
Table of Contents: Introduction
Table of Contents: Introduction

... comparison to neighboring world regions using autosomal STR data. In particular, this analysis will explore evidence for early migrations to Europe from West Asia (including Anatolia and the East Mediterranean) and Siberia (including early relatives of Native Americans). The background section highl ...
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA
Clinical and Molecular Aspects of Diseases of Mitochondrial DNA

... July-August 2009 ...
An Infectious Topic in Reticulate Evolution: Introgression
An Infectious Topic in Reticulate Evolution: Introgression

... interest because host or parasite hybridization may impact host resistance/susceptibility or parasite infectivity, virulence, transmission, or host specificity. The first major synthesis of the role of hybridization in host-parasite interactions was presented in a review by Fritz et al. [4], and an ...
Restriction mapping
Restriction mapping

... destructive enzymes. REs are part of a bacterial defense system against foreign DNA, such as an infectious bacteriophage. The RE sites in the bacterium’s own DNA are protected from cleavage because they have been modified by a methyltransferase that specifically modifies the RE sites. The combined a ...
video slide
video slide

Automatic identification of topic boundaries in
Automatic identification of topic boundaries in

... sequence. In some cases, indentifying such local alignments proves useful. The Smith and Waterman algorithm (Waterman 1984) finds an alignment that determines the longest/best subsequence pair that gives maximum degree of similarity between the two sequences. Single sequence alignment conveys whethe ...
Prolonged organ retention and safety of plasmid DNA
Prolonged organ retention and safety of plasmid DNA

... deletion mutant competitor. Moreover, we revealed the histopathology of the organs after repeated dosing, indicating the conditional safety of PEI/DNA complexes. After administration of DNA in PEI complexes, the serum levels of DNA rapidly decreased in the initial phase, and then continued to decrea ...
Identification of Bacterial Species Using Colony PCR
Identification of Bacterial Species Using Colony PCR

Low Levels of Nucleotide Diversity at Homoeologous Adh Loci in
Low Levels of Nucleotide Diversity at Homoeologous Adh Loci in

... to be ruled out as having contributed to differential evolution. The cotton genus (Gossypium) provides a model system for studying molecular evolution of genes duplicated by allopolyploidy. The five tetraploid Gossypium species (n 5 26) are a monophyletic assemblage derived from a single allopolyplo ...
Genetic diversity and differentiation in Camellia reticulata - Funpec-RP
Genetic diversity and differentiation in Camellia reticulata - Funpec-RP

... Intersimple sequence repeat (ISSR) is a polymerase chain reaction (PCR)-based technique that amplifies sequences between adjacent, inversely oriented microsatellites using an SSR-containing primer (Zietkiewicz et al., 1994). The strategy is especially attractive because it avoids the need to carry o ...
From Genetics to DNA
From Genetics to DNA

... pull apart. In the laboratory, the strength of this interaction can be measured by finding the temperature required to break the hydrogen bonds, their melting temperature (also called Tm value). When all the base pairs in a DNA double helix melt, the strands separate and exist in solution as two ent ...
Capillary Electrophoresis of Oligonucleotides
Capillary Electrophoresis of Oligonucleotides

... length of an oligonucleotide can be estimated by CE using known size standards. For example, the size standard used by IDT is a purified 40-mer that is used to define a retention time for the capillary. For example, if the 40-mer standard has a retention time of 22 minutes and the average time betwe ...
Frequently Asked Questions: Agarose Gel Electrophoresis
Frequently Asked Questions: Agarose Gel Electrophoresis

P. roridus - Longwood Blogs
P. roridus - Longwood Blogs

... compiled data from scientists such as Grove and Hu (Foos et al. 2011), the species the spores originated from could be hypothesized. However, due to spore-size overlaps and natural distinctive characteristics among individual strains, this method is inconclusive, leaving an uncertainty of identity a ...
Catellibacterium aquatile sp. nov., isolated from fresh water, and
Catellibacterium aquatile sp. nov., isolated from fresh water, and

on January 24, 2017 Downloaded from
on January 24, 2017 Downloaded from

... Published January 1, 1965 ...
"PCR-based Species Identification and Gene Tagging"
"PCR-based Species Identification and Gene Tagging"

... maize leaf _Bots infected leaf pt3 infected leaf pt1 P sorghi pt3 P sorghi pt1 Marker ...
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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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