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Comparative In silico Study of Sex
Comparative In silico Study of Sex

... study, SRY of 15 spices included of human, chimpanzee, dog, pig, rat, cattle, buffalo, goat, sheep, horse, zebra, frog, urial, dolphin and killer whale were used for determine of bioinformatic differences. Methods: Nucleotide sequences of SRY were retrieved from the NCBI databank. Bioinformatic anal ...
Using the Wolbachia Bacterial Symbiont to Teach Inquiry
Using the Wolbachia Bacterial Symbiont to Teach Inquiry

... THE WOLBACHIA BACTERIAL SYMBIONT ...
Mitochondrial point mutations do not limit the natural lifespan of mice
Mitochondrial point mutations do not limit the natural lifespan of mice

... (Fig. 1a). However, subtle fluctuations from these averages may occur between cell types within tissues. Mutation frequencies determined via the RMC assay in young mice are approximately one to two orders of magnitude lower than previously documented by conventional methods7,13. Although the RMC ass ...
Structure of B-DNA with Cations Tethered in the Major Groove†
Structure of B-DNA with Cations Tethered in the Major Groove†

... the amino-propyl groups, is well-determined by the data (Figure 2). The occupancies of the two terminal O5′ atoms of the DNA were set to zero because they appear to be disordered; there is no electron density observed around them. Each of the four amino-propyl modifications is readily identifiable i ...
Microsynth GmbH
Microsynth GmbH

... proven protocols and an overall experience of 25 years in the area of Sanger sequencing usually result in long reading lengths and clean sequences. Nevertheless, there are factors, which can affect the successful generation of a high quality sequence. The reasons for that are of different nature. So ...
Bioinformatics - Sequences and Computers
Bioinformatics - Sequences and Computers

... According to the concept of evolution (and our own experience) living organisms can be traced back to forebears. Going back further and further in time allows to connect currently distinct species through common ancestors until, ultimately, all life might spring from a few or even one ancestral "cel ...
Trouble Shooting Guide
Trouble Shooting Guide

... usually result in long reading lengths and clean sequences. Nevertheless, there are factors, which can affect the successful generation of a high quality sequence. The reasons for that are of different nature. Some causes can already be identified at the electropherogram level, while for others addi ...
(COX2) gene in giant panda, Ailuropoda melanoleuca
(COX2) gene in giant panda, Ailuropoda melanoleuca

... complete mitochondrial genome of giant panda as reported (Peng et al., 2007) and with those deposited in GenBank, as the BLAST results revealed 100% sequence identity. Although some mutations were detected in the COX2 sequences, none of those mutations caused changes in the amino acids, and hence th ...
The rapidly evolving field of plant centromeres
The rapidly evolving field of plant centromeres

... flanked by DNA that is rich in middle repetitive elements, including transposons, retroelements and pseudogenes (Figure 1). The middle repetitive DNA is often referred to as the pericentromere, although domains in this region may contribute to centromere activity. Although centromere functions are h ...
A molecular method for assessing meiofauna diversity in marine
A molecular method for assessing meiofauna diversity in marine

... found that using meiofauna data along with macrofauna data provides greater insight into the processes affecting a polluted area, since meiofauna are not as affected by physical disturbance as macrofauna. The cost of processing and identifying macrofauna is high and incorporating another component o ...
Chapter 1
Chapter 1

... fatty acids (hydrocarbons with a carboxyl-group, COOH, on one end) will spontaneously form vesicles in water: multiple molecules align into a spherical structure so that the carboxyl-group faces out and the hydrophobic lipid tail is shielded away from the bipolar water molecules. A cell membrane is ...
Phylogenetic analysis of the insect order Odonata using 28S and
Phylogenetic analysis of the insect order Odonata using 28S and

... suggested that it is actually a relict of the early branching of the Anisozygoptera from the other two suborders. A phylogenetic analysis of the entire Insecta using 18S rDNA supports the viewpoint of Nel et al. (Kjer 2004); however, Kjer noted that the sequence diversity of this gene was very low i ...
Modeling Spatial Correlation of DNA Deformation
Modeling Spatial Correlation of DNA Deformation

... incorporates the correct amount of local details while at the same time provides the computational efficiency for relatively long chains of DNA. An excellent example is a recent experimental single-molecule study by Kim et al.,11 which has motivated the present study. In this experiment, a single DNA ...
FastGene Taq DNA Polymerase
FastGene Taq DNA Polymerase

... assays. For GC-rich targets (>65% GC content) 5 min at 95 °C may be used. ...
video slide - apbiologyclass
video slide - apbiologyclass

... Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings ...
Contribution of X chromosomal and autosomal genes to species
Contribution of X chromosomal and autosomal genes to species

... however, not possible due to multiple chromosome rearrangements restricting recombination between RFLP markers located on this area. The same problem was faced when studying hybrids between D. flavomontana and D. montana with less diverged X chromosomal gene arrangements. Interaction between the X c ...
MONOHYBRID CROSS
MONOHYBRID CROSS

Bacterial genospecies that are not ecologically
Bacterial genospecies that are not ecologically

... [4– 6]. Alternatively, these clusters might reflect the underlying ecological niches provided by the environment, and this idea has been developed into the ecotype model, in which genotypic clusters map onto ecological niches and periodic selective sweeps purge genetic variation within each niche se ...
A Brief History of PCR - Bio-Rad
A Brief History of PCR - Bio-Rad

Full Text
Full Text

... prediction because of their higher conservation when compared with equivalent DNA sequences; (ii) it minimizes the number of false positives caused by sometimes inconsistent hits ranking produced by local sequence alignment tools such as BLAST; and (iii) it ensures that sequence similarities obtaine ...
2.4. Sequence databases
2.4. Sequence databases

... tables and combines the information into one report. Therefore, information can be found more quickly. Relational databases can be created using a special programming language called structured query language (SQL). The creation of this type of databases can take a great deal of planning during the ...
Candidate gene analysis of thyroid hormone receptors
Candidate gene analysis of thyroid hormone receptors

... (1996), a study that examined mitochondrial DNA (mtDNA) variation among ambystomatid (A. tigrinum complex) populations and species, including all know metamorphic-failure populations from Mexico. Table 1 shows the population origin of these samples, as well as the life cycle mode that characterizes ...
Chapter Outline
Chapter Outline

... • The near uniformity of the rate of molecular  evolution in different lineages is metaphorically evolution in different lineages is metaphorically  described as a “molecular clock.” • The rate of evolution varies among different protein  and DNA sequences and appears to depend on the  extent to whi ...
Teacher`s guide - National Centre for Biotechnology Education
Teacher`s guide - National Centre for Biotechnology Education

... There are two ways in which the exercise may be used. It can be used to illustrate a true medical condition and its inheritance, such as the single base change (point mutation) that can cause to sickle cell anaemia. Alternatively, if teachers do not wish to a ‘real’ condition and the various concern ...
Identification of genes that are associated with DNA repeats in
Identification of genes that are associated with DNA repeats in

... contrast, cas homologues were absent from any of the completely sequenced CRISPR-negative genomes, illustrating the strong association of cas genes and CRISPR loci (see Table 1). No CRISPR loci were identified in eukaryotic genomes and, as expected, no homologues of the cas genes were found in these ...
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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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