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A small organic compound enhances the religation reaction of
A small organic compound enhances the religation reaction of

... The different steps of the human Top1 (topoisomerase I) catalytic cycle have been analysed in the presence of a pentacyclic-diquinoid synthetic compound. The experiments indicate that it efficiently inhibits the cleavage step of the enzyme reaction, fitting well into the catalytic site. Surprisingly ...
Killing specimens
Killing specimens

... wings. Also be sure that specimens are not able to chew their way out of the container. 3. Food: If possible, host plant material should be exchanged regularly with fresh material. For internal feeders, such as leaf miners or seed borers this is not an option. In these cases, great care should be ta ...
Plasmodium malariae and P. ovale genomes provide insights into
Plasmodium malariae and P. ovale genomes provide insights into

Mitochondrial genes in the colourless alga Prototheca wickerhamii
Mitochondrial genes in the colourless alga Prototheca wickerhamii

... indicated by its bootstrap values (all above 93%). Consistent results were obtained by using informative positions varying in numbers between 453 and 98 or by applying further algorithms like a maximum likelihood approach and a neighbour-joining method (35). Two main aspects of the presented tree ca ...
Geneious Sequence Classifier User Manual
Geneious Sequence Classifier User Manual

... sequence will be classified to the taxonomic level that these database sequences have in common. E.g. for the example dataset given above with ”classify using taxonomy” checked, if this value is set on 0.2%, and the query sequence has 99.3% identity with Acmopyle pancheri and 99.2% identity with Acm ...
dna biometrics - Danish Biometrics
dna biometrics - Danish Biometrics

... Network Security which can be used synonymously with computer and information security as being the safe guarding of digital information stored on a magnetic or optical drive which is transmitted over a network. Through various ways, employees and key personnel can access these vital resources but t ...
24 - Lab Times
24 - Lab Times

... are like the measles – everyone gets it and sic barrier and evolve intrinsic (genetic) rethe environment are the starting point and we all get over it!”. Since we know today productive isolation, such that if the barrithat this goes along with the development of that it is actually not very difficul ...
Crystal structure of the nucleosome core particle at 2.8 Å
Crystal structure of the nucleosome core particle at 2.8 Å

... the DNA superhelix axis for the left particle and perpendicular to it for the right particle. For both particles, the pseudo-twofold axis is aligned vertically with the DNA centre at the top. b. DNA phosphate B-factors versus base pair. The sequence of the DNA used is shown with corresponding B-fact ...
Systematic and Applied Microbiology
Systematic and Applied Microbiology

... Koser citrate medium and were non-motile. Adonitol is generally utilized and fermented by Klebsiella [18] but the three banana isolates F2R9T, 6A2 and VI, strain T29A from sugar cane, strain 3 from maize, strain CFNE 2006 from rice and 7 of the 131 clinical isolates (5.3% of the total) did not ferme ...
Sequences of Primate Insulin Genes Support
Sequences of Primate Insulin Genes Support

... Biochemistry and Molecular Biology, The University of Chicago; and *Center for Demographic and Population Genetics, University of Texas at Houston ...
Functional analysis of plastid DNA replication origins in tobacco by
Functional analysis of plastid DNA replication origins in tobacco by

... B, respectively, is shown on top of the plasmids; regions of homology are indicated by dotted lines. Genes are indicated as open arrows marking the direction of transcription. JSA and JSB denote the border between Inverted Repeat A or B, respectively, and the small single copy region of the plastome ...
DNA Testing - Who Murdered Robert Wone
DNA Testing - Who Murdered Robert Wone

... millions or billions of DNA molecules in about three hours. The PCR process is sometimes compared to a Xerox machine since many copies are made. While initially, this is a useful comparison, it doesn't communicate the true, chain-reaction nature of PCR. In PCR, the original DNA is copied, then the c ...
The replication of DNA
The replication of DNA

... a primer strand. • Increased processivity is facilitated by the ability of DNA polymerases to slide along DNA template. Each time a nucleotide is added the DNA partially release from the polymerase (H-bond with the minor groove are broken, but the electrostatic interaction with the thumb are maintai ...
Molecular Phylogenetics Basis of Phylogenetics
Molecular Phylogenetics Basis of Phylogenetics

... ¾ over time, this process may repeat itself, so that at any time, each population can be said to be most closely-related to some other population with which it shares a direct common ancestor If genomes evolve the by accumulation of mutations ¾ the amount of nucleotide sequence difference between a ...
Evidence for Variable Selective Pressures at a
Evidence for Variable Selective Pressures at a

... The increasing amount of available sequences revealed a similar control region structural organization across mammals, with size variation ranging between 880 and 1400 bp (Sbisa et al. 1997). One important issue relating to these observations is thus to identify which structural and/or functional do ...
Extrachromosomal DNA Transformationof Caenorhabditis elegans
Extrachromosomal DNA Transformationof Caenorhabditis elegans

... foreign DNA sequences in the transformed worm cells were measured by quantitative hybridization analysis. DNA preparations from transformed populations were spotted onto triplicate nitrocellulose filters. The filters were hybridized with 32P-labeled pBR322 DNA, bacteriophage DNA containing a single- ...
fulltext - DiVA portal
fulltext - DiVA portal

... distribution pattern called geographical parthenogenesis. Using molecular phylogeny, I here examine the evolution of Otiorynchid weevils, mainly Otiorhynchus scaber and sulcatus in an attempt to trace the evolutionary history and find out what causes the variation in success of different parthenogen ...
Processivity of DNA polymerases: two mechanisms, one goal
Processivity of DNA polymerases: two mechanisms, one goal

... polymerase to the DNA. Future structural studies of replicases and other proteins involved in chromosome replication are sure to bring to light new and exiting mechanisms for handling the DNA helix. ...
Restriction Enzyme Digest and Plasmid mapping
Restriction Enzyme Digest and Plasmid mapping

... This excercise introduces you to some important principles of genetic engineering. Specifically, the functions of restriction enzymes and their use as molecular biology tools will be stressed. Using agarose gel electrophoresis, you will examine the digestion patterns and determine the sizes of unkno ...
calibration of tethered particle motion experiments
calibration of tethered particle motion experiments

... sets with relative standard deviation u < 0.2 are accepted. For example, the bead shown in Fig. 2(c) meets the motion and symmetry criterions; however, it displays a non-specific binding event at 30s. In short, our third criterion removes tethers with temporal inconsistency in their Brownian motion. ...
Isogamous, hermaphroditic inheritance of mitochondrion
Isogamous, hermaphroditic inheritance of mitochondrion

A disproportionate role for mtDNA in DobzhanskyMuller
A disproportionate role for mtDNA in DobzhanskyMuller

... of the plant literature on nuclear–plastome incompatibilities and their role in speciation. The presence of the chloroplast genome in plants (in addition to the mtDNA), adds further potential for intergenomic interactions. Like mitochondria, chloroplasts derive from an ancient endosymbiosis, in this ...
Primer design - ILRI Research Computing
Primer design - ILRI Research Computing

... Degenerate primers are widely used and extremely useful in the field of microbial ecology. They allow for the amplification of genes from thus far uncultivated microorganisms or allow the recovery of genes from organisms where genomic information is not available. Usually, degenerate primers are des ...
The Nucleotide Sequence Determination of Catalases of Three
The Nucleotide Sequence Determination of Catalases of Three

... the 5’ untranscribed region of catalase genes of the three species and determined their sequences. Table 2 shows homology scores among catalases of known yeast species. The homology between the C. albicans and C. dubliniensis catalase is 99% in amino acid, indicating that the two species are closely ...
Enzyme Mechanisms - Illinois Institute of Technology
Enzyme Mechanisms - Illinois Institute of Technology

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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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