Overexpression of the catalytic subunit of DNA polymerase results in
... biogenesis program, and terminally differentiated cells, particularly those with higher oxidative load, do not receive the correct number of functional mitochondria to meet their demand for energy production. To gain further insight into the physiological regulation of the mtDNA replication process, ...
... biogenesis program, and terminally differentiated cells, particularly those with higher oxidative load, do not receive the correct number of functional mitochondria to meet their demand for energy production. To gain further insight into the physiological regulation of the mtDNA replication process, ...
Sheared DNA fragment sizing: comparison of techniques
... For some experiments it is necessary to use long (>1000 base pair) fragments of DNA. Fragments of this size have been used to determine the extent of inter2 3 4 sperslon of repeated and nonrepeated sequences of DNA in eucaryotes ' ' In general, however, on kinetic and other grounds it is advantageou ...
... For some experiments it is necessary to use long (>1000 base pair) fragments of DNA. Fragments of this size have been used to determine the extent of inter2 3 4 sperslon of repeated and nonrepeated sequences of DNA in eucaryotes ' ' In general, however, on kinetic and other grounds it is advantageou ...
Electrophoresis, Blotting and Immunodetection Gel
... Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit. A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares gel-purified PCR products for sequencing or cloning without need for further purification. Device volum ...
... Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit. A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares gel-purified PCR products for sequencing or cloning without need for further purification. Device volum ...
Application of rpoB sequence similarity analysis, REP‐PCR and
... 2004). Therefore, studying phylogenetic relations and diversity in this novel bacterial genus is not only a taxonomical concern, but also a necessity in order to exploit its biotechnological potential completely. ...
... 2004). Therefore, studying phylogenetic relations and diversity in this novel bacterial genus is not only a taxonomical concern, but also a necessity in order to exploit its biotechnological potential completely. ...
OCCASIONAL PAPERS OF THE MUSEUM OF ZOOLOGY
... Statistical analyses were performed to help characterize the shape of the central lateral spot within each sample. A sign test was used to determine if the spot was generally long or generally deep by assessing whether the number of individuals in which the spot was longer than deep (or deeper than ...
... Statistical analyses were performed to help characterize the shape of the central lateral spot within each sample. A sign test was used to determine if the spot was generally long or generally deep by assessing whether the number of individuals in which the spot was longer than deep (or deeper than ...
Level 3, 2004
... the order, and how many of them are linked together, determines what the protein is and does. These amino acid sequences are coded for by sequenced triplets of bases on the DNA. If the DNA has a large part of the sequence replaced by another sequence, then it usually forms a completely different pro ...
... the order, and how many of them are linked together, determines what the protein is and does. These amino acid sequences are coded for by sequenced triplets of bases on the DNA. If the DNA has a large part of the sequence replaced by another sequence, then it usually forms a completely different pro ...
Factors affecting the amount of genomic DNA
... Frantz et al. 2003). These studies not only represent multiple species with varied diets and living conditions, but also employ various sample storage, DNA extraction and PCR amplification methods. Furthermore, use of the average frequency with which PCR products are obtained as a measure of success ...
... Frantz et al. 2003). These studies not only represent multiple species with varied diets and living conditions, but also employ various sample storage, DNA extraction and PCR amplification methods. Furthermore, use of the average frequency with which PCR products are obtained as a measure of success ...
Neanderthal-human Hybrids
... interchanged with human autosomes, eventually losing their identity in the process. In a scenario previously not considered by other researchers (29), if male Neanderthals mated with female humans, Haldane’s law would account for the apparent absence of Neanderthal Y-linked genes in the human popula ...
... interchanged with human autosomes, eventually losing their identity in the process. In a scenario previously not considered by other researchers (29), if male Neanderthals mated with female humans, Haldane’s law would account for the apparent absence of Neanderthal Y-linked genes in the human popula ...
Evolutionary implications of non- neutral
... Studies that seek to demonstrate the fitness consequences of mitochondrial genetic variation are complicated by the inherent difficulty of disentangling the effects of mitochondrial genes from those of other genomes. At the very least, it is essential to control for the nuclear background. One simpl ...
... Studies that seek to demonstrate the fitness consequences of mitochondrial genetic variation are complicated by the inherent difficulty of disentangling the effects of mitochondrial genes from those of other genomes. At the very least, it is essential to control for the nuclear background. One simpl ...
laboratory methods for diagnosis and detection of
... based on the sequence of mtp40 has been identified as part of the mpcA gene. This gene encodes a phospholipase C region of MTB and carries a particular mutation at position 169 of the gene conferring pyrazinamide resistance(35). Although originally thought to be specific for MTB(36), subsequent stud ...
... based on the sequence of mtp40 has been identified as part of the mpcA gene. This gene encodes a phospholipase C region of MTB and carries a particular mutation at position 169 of the gene conferring pyrazinamide resistance(35). Although originally thought to be specific for MTB(36), subsequent stud ...
anovel,validated method for absolute
... predicted, such as the amount of delayed onset, and is accurate to within 5%. If there are no changes in primer design, primer concentration, or the master mix, then this assay calibration is sufficient for all future samples. Alternatively, DNA Software has developed fluorescence-prediction procedu ...
... predicted, such as the amount of delayed onset, and is accurate to within 5%. If there are no changes in primer design, primer concentration, or the master mix, then this assay calibration is sufficient for all future samples. Alternatively, DNA Software has developed fluorescence-prediction procedu ...
Use of Taxonomy in Risk Assessment of Micro
... alone suffice for either classification or identification of some bacteria, but it is possible to combine these methods using polyphasic taxonomy. Experience with all of the above techniques reveals that no single method is perfect for all taxa and all levels of taxonomic hierarchy. Using bacterial ...
... alone suffice for either classification or identification of some bacteria, but it is possible to combine these methods using polyphasic taxonomy. Experience with all of the above techniques reveals that no single method is perfect for all taxa and all levels of taxonomic hierarchy. Using bacterial ...
Comparison of DNA Sequences with Protein Sequences
... sequencing. The DNA sequences produced by single-pass EST sequencing and high-throughput sequencing may be of lower quality than traditional ‘‘finished’’ GenBank sequences, which are typically based on multiple sequence reads from both strands of the DNA template. As a result, EST sequences are more ...
... sequencing. The DNA sequences produced by single-pass EST sequencing and high-throughput sequencing may be of lower quality than traditional ‘‘finished’’ GenBank sequences, which are typically based on multiple sequence reads from both strands of the DNA template. As a result, EST sequences are more ...
Identification and quantification of mycotoxigenic fungi
... extended to equal the number of kb of the product to be amplified and to yield doubled DNA. The process is cycled approximately 40 times to give the desired quantity of DNA product. The use of the thermophilic polymerases involved in the reactions is of course revolutionary. The DNAs are separated o ...
... extended to equal the number of kb of the product to be amplified and to yield doubled DNA. The process is cycled approximately 40 times to give the desired quantity of DNA product. The use of the thermophilic polymerases involved in the reactions is of course revolutionary. The DNAs are separated o ...
Article Mitochondrial DNA turnover occurs during preimplantation
... Bio-Rad Radiance confocal microscope (Biorad House, Hertfordshire, UK) all samples were measured at identical settings. At least 20 embryos were collected for each stage. One-cell embryos were cultured in aphidicolin for 5 h and mtDNA copy number was measured in control and treated groups. No differ ...
... Bio-Rad Radiance confocal microscope (Biorad House, Hertfordshire, UK) all samples were measured at identical settings. At least 20 embryos were collected for each stage. One-cell embryos were cultured in aphidicolin for 5 h and mtDNA copy number was measured in control and treated groups. No differ ...
Genetic, morphological, and chemical patterns of plant hybridization
... participating populations and contributing to adaptive evolution. Hybridization by this time was considered as a very frequent phenomenon in flowering plants (Stebbins 1959). In the last decades, the high number of molecular markers used to document hybridization have permitted to confirm several of ...
... participating populations and contributing to adaptive evolution. Hybridization by this time was considered as a very frequent phenomenon in flowering plants (Stebbins 1959). In the last decades, the high number of molecular markers used to document hybridization have permitted to confirm several of ...
hybrid DNA molecules
... of the cloned his3 gene that delete DNA sequences near or in the structural gene have been isolated and physically defined (unpublished data). The demonstration by Hinnen et al. (5) that recombinant DNA containing cloned yeast genes can be used to transform yeast cells clearly expands the potential ...
... of the cloned his3 gene that delete DNA sequences near or in the structural gene have been isolated and physically defined (unpublished data). The demonstration by Hinnen et al. (5) that recombinant DNA containing cloned yeast genes can be used to transform yeast cells clearly expands the potential ...
Compiling DNA strand displacement reactions using a functional
... Previous work has shown that synthetic DNA circuits can be used to implement computational systems including digital logic circuits [1], neural networks [2] and gameplaying automata [3]. In this setting, DNA is used both as an information carrier and as an engineering material, simultaneously. Furth ...
... Previous work has shown that synthetic DNA circuits can be used to implement computational systems including digital logic circuits [1], neural networks [2] and gameplaying automata [3]. In this setting, DNA is used both as an information carrier and as an engineering material, simultaneously. Furth ...
Recombinant DNA Lesson - Ms. Guiotto Biology Class
... Unit I • Fundamentalsof Biotechnology The hydrogen bonds release, and a fragment is extracted from cells of the same organism, from a generated. Many restriction enzymes are known culture of bacteria, or from a strain of viruses. Afwhich cut at different recognition sequences, and ter the DNA is di ...
... Unit I • Fundamentalsof Biotechnology The hydrogen bonds release, and a fragment is extracted from cells of the same organism, from a generated. Many restriction enzymes are known culture of bacteria, or from a strain of viruses. Afwhich cut at different recognition sequences, and ter the DNA is di ...
