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Next-generation DNA sequencing techniques
Next-generation DNA sequencing techniques

... companies, ABI (commercialising the Kambara system) and Amersham (taking over and developing further the system set up in the US by the Molecular Dynamics company), commercialised automated sequencing using parallel analysis in systems of up to 384 capillaries at that time. Together with partial min ...
An Approximate Approach to DNA Denaturation
An Approximate Approach to DNA Denaturation

... Let us briefly review the development of experimental work t h a t has been done in recent years. The experimental background comes mainly from R a m a n and IR spectroscopic results t h a t are an alternative approach (Urabe and Tominaga 1981), more closely connected to theoretical lattice dynamics ...
DNA Hybridization: A Decade of Molecular Discourse in Hominoid
DNA Hybridization: A Decade of Molecular Discourse in Hominoid

... molecular evidence for a Pan-Homo clade. Based on the 183 DNA hybrids that were formed, the researchers interpreted the evidence to mean that the branching order, from oldest to most recent, was gibbons, orangutan, gorilla, chimpanzees, and human, as illustrated in Figure 1.1 (Sibley and Ahlquist 19 ...
Lab Meeting, Oct 16 2003
Lab Meeting, Oct 16 2003

The Use of Cytochrome B Sequence Variation in Estimation of
The Use of Cytochrome B Sequence Variation in Estimation of

... and at least four times larger than mean distances among specieswithin the passerinegenera, Melospiza, Dendroica and Parus, studied to date (see Tegelstrom and Gelter 1990, for a review of genetic distances). This large distance may be due to two factors which are not mutually exclusive. The taxon m ...
Identification of large-scale human-specific copy number
Identification of large-scale human-specific copy number

... pool, also consisting of ten unrelated (female) individuals. In order to validate the aCGH procedure, a control experiment was performed using genomic DNA from one (female) individual per species that had not been included in the genomic DNA pool. No significant differences were observed between the r ...
this help page as PDF
this help page as PDF

... Scipio is able to reconstruct genes that are spread on several contigs or supercontigs of highly fragmented genomes. As we have shown, this feature is one of the most important strengths of Scipio that other programs do not offer. However, this feature is not needed in chromosomal assemblies, and mi ...
Amplification of a DNA Fragment Using Polymerase
Amplification of a DNA Fragment Using Polymerase

... chromosomal DNA, which often require larger biological samples and are less discriminating. Polymerase chain reaction has also proven to be useful in the fields of archaeology and evolution. Ancient biological samples recovered from digs and expeditions can be subjected to PCR to gain insight into t ...
Evolution of the chromosomal location of rDNA genes in
Evolution of the chromosomal location of rDNA genes in

... strong in D. sechellia and weak in D. simulans, whereas the reverse is observed for the Y hybridization sites (boxes in Figure 1a and b). In D. mauritiana, the heterochromatic structure of the Y chromosome differs greatly from that observed in D. sechellia and D. simulans (Figure 2a). The Py12 probe ...
MBoMS Genomics of Model Microbes Lab 3: Tools for
MBoMS Genomics of Model Microbes Lab 3: Tools for

Full-Text PDF
Full-Text PDF

... considerable algorithmic challenges, which gave rise to (often unnatural) constraints on these models, even for conceptually simple tasks such as the calculation of distance between two structures or the identification of UCEs. These constraints are now being addressed with fast and efficient soluti ...
Southern Blotting and Related DNA Detection Techniques
Southern Blotting and Related DNA Detection Techniques

... membrane. The major improvement has been the introduction of nylon membranes, which have three advantages over their nitrocellulose counterparts. First, nylon membranes are less fragile than nitrocellulose sheets, the latter tending to crack if handled roughly during Southern blotting, and usually d ...
Natural Transfer of Conjugative Transposon Tn916 between Gram
Natural Transfer of Conjugative Transposon Tn916 between Gram

... circular form postulated earlier as a transposition intermediate has been recently shown (32). This fertility potential could explain the transfer of Tn9J6 from pAM120 to grampositive bacteria. The remaining part of this plasmid is a pBR322 derivative and does not contain any tra genes. The quantita ...
Package `miRNAtap`
Package `miRNAtap`

... in this case same as keytype, the table in which the search should be performed, this value specifies the source of predictions as well as species; as with keys, all possible columns are returned by using the columns method. ...
Using the Basic Local Alignment Search Tool (BLAST) - bio-bio-1
Using the Basic Local Alignment Search Tool (BLAST) - bio-bio-1

... sequence similarity searches by an algorithm that is faster than FASTA but considered to be equally as sensitive. Both of these methods follow a heuristic (tried-and-true) method that almost always works to find related sequences in a database search, but does not have the underlying guarantee of an ...
Comparison Between Currently Used Blood Samples And New
Comparison Between Currently Used Blood Samples And New

... avenues of testing and research, but it can also be a time consuming, expensive and invasive collection method - especially for long term or broad range studies. Scientist are trying to find a comparable source of genetic material, such as saliva, that is more cost effective, more stable and less in ...
Full text - terrapub
Full text - terrapub

... high diversity in habitat preference, but low bloom species diversity (behavior) within these habitats. Diatoms have the opposite pattern: low habitat diversity, but high bloom-species diversity. The similar diatom bloom-species composition found across the dinoflagellate life-form habitats suggests ...
IOSR Journal of Applied Physics (IOSR-JAP)
IOSR Journal of Applied Physics (IOSR-JAP)

cached copy
cached copy

... other strands but also the detailed shape of the joined segments. Since the mid-1990s, it has been possible to program the shapes of DNA branched species using only their sequences. We do not have such specific information for proteins or antibodies, which are other candidates for working elements. T ...
BEETLE RECORDS | Overview - Royal Holloway, University of
BEETLE RECORDS | Overview - Royal Holloway, University of

... species. A full discussion of this topic can be found elsewhere in this encyclopedia. The second line of evidence for Quaternary species constancy comes from the stability of shape and size of fossil aedeagae, as discussed in section ’Exoskeletal Parts Preserved in Quaternary Deposits,’ above. The t ...
Lecture 4: Lecture Notes + Textbook
Lecture 4: Lecture Notes + Textbook

... remember, there are 4 reaction mixtures, so 1 mixture will have the adenine form of the dideoxy analog, another will have the guanine form, and the other two will have thymine and cytosine) Normally, the polymerase adds a complementary base, then moves to the next nucleotide and does the same Howeve ...
16 • Bad species
16 • Bad species

... (Barton & Hewitt 1989, Berlocher 1998, Coyne & Orr 2004) – practising taxonomists often continue to use syndiagnostic methods based mainly on morphological characters. Indeed, when taxonomists have a sample of specimens coming from an unexplored geographical area, they can find morphological differen ...
Chapter 16 The Molecular Basis of Inheritance
Chapter 16 The Molecular Basis of Inheritance

Agrobacterium-mediated DNA transfer, and then some
Agrobacterium-mediated DNA transfer, and then some

... dreds of thousands of T-DNA insertion mutations in the genomes of model plant species, such as Arabidopsis thaliana (thale cress) and Oryza sativa (rice). These T-DNA insertion libraries, intended to saturate the genome with mutations, are important tools for forward and reverse genetic studies to u ...
Identification of Short Motifs for Comparing Biological Sequences
Identification of Short Motifs for Comparing Biological Sequences

... [email protected] Abstract — Sequence comparison remains one of the main computational tools in bioinformatics research. It is an essential starting point for addressing many problems in bioinformatics; including problems associated with recognition and classification of organisms. Although sequence ...
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DNA barcoding

DNA barcoding is a taxonomic method that uses a short genetic marker in an organism's DNA to identify it as belonging to a particular species. It differs from molecular phylogeny in that the main goal is not to determine patterns of relationship but to identify an unknown sample in terms of a preexisting classification. Although barcodes are sometimes used in an effort to identify unknown species or assess whether species should be combined or separated, the utility of DNA barcoding for these purposes is subject to debate.The most commonly used barcode region, for animals, at least, is a segment of approximately 600 base pairs of the mitochondrial gene cytochrome oxidase I (COI).Applications include, for example, identifying plant leaves even when flowers or fruit are not available, identifying insect larvae (which may have fewer diagnostic characters than adults and are frequently less well-known), identifying the diet of an animal, based on its stomach contents or faeces and identifying products in commerce (for example, herbal supplements, wood, or skins and other animal parts).
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