Using LIMS for Flexible Information Management at a Bovine DNA
... calving errors to be 20% and above. Approximately one in six calves has the potential to be mis-mothered, mis-tagged or mis-recorded. Inaccuracies such as this can result in inbreeding problems in herds, which can cost up to NZ$1000 per animal in lost production, and the most at-risk animals for inb ...
... calving errors to be 20% and above. Approximately one in six calves has the potential to be mis-mothered, mis-tagged or mis-recorded. Inaccuracies such as this can result in inbreeding problems in herds, which can cost up to NZ$1000 per animal in lost production, and the most at-risk animals for inb ...
Section 7.1 DNA Cloning with Plasmid Vectors
... The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. In the case of DNA, this is feasible for relatively short molecules such as the genomes of small viruses. But genomes of even the simplest cells are much too large to dir ...
... The essence of cell chemistry is to isolate a particular cellular component and then analyze its chemical structure and activity. In the case of DNA, this is feasible for relatively short molecules such as the genomes of small viruses. But genomes of even the simplest cells are much too large to dir ...
Natural genetic transformation: prevalence, mechanisms
... natural transformation in Gram-positive bacteria. In both genera, the competence genes can be divided into two groups; those involved in deciding when conditions are right for development of the competent state (early genes) and those required for DNA binding, import and recombination (late genes). ...
... natural transformation in Gram-positive bacteria. In both genera, the competence genes can be divided into two groups; those involved in deciding when conditions are right for development of the competent state (early genes) and those required for DNA binding, import and recombination (late genes). ...
Comparison of real-time PCR with SYBR Green I or
... was shown to detect a 10 % target DNA fraction present in mixed DNA samples. Applicability of the rDNA-targeted oligonucleotide probes without pre-enrichment of the 16S gene pool by PCR was thus limited to the detection of the predominant microbial groups. Real-time PCR was performed using a 96-well ...
... was shown to detect a 10 % target DNA fraction present in mixed DNA samples. Applicability of the rDNA-targeted oligonucleotide probes without pre-enrichment of the 16S gene pool by PCR was thus limited to the detection of the predominant microbial groups. Real-time PCR was performed using a 96-well ...
A natural chimeric yeast containing genetic material from three species
... al., 1985). The ATP9 genes in the isolates analysed were also found to be 228 bp long (Fig. 2). The amino acid sequences were identical in all cases, but several silent substitutions were observed. The ATP9 gene sequences of Saccharomyces sp. CID1 and Saccharomyces sp. IFO 1802 were identical. Also, ...
... al., 1985). The ATP9 genes in the isolates analysed were also found to be 228 bp long (Fig. 2). The amino acid sequences were identical in all cases, but several silent substitutions were observed. The ATP9 gene sequences of Saccharomyces sp. CID1 and Saccharomyces sp. IFO 1802 were identical. Also, ...
Phenotypic and Molecular Identification of Bifidobacterium sp
... 3-Molecular identification of bifidobacteria and amplification of xfp gene Genomic DNA was prepared according to the procedure of Kate Wilson 1997. Its briefly occur by Incubattion approximately 5 ml of liquid culture media with bacteria at optimum condition of growth for 24 hr. transfer 1.5ml of cu ...
... 3-Molecular identification of bifidobacteria and amplification of xfp gene Genomic DNA was prepared according to the procedure of Kate Wilson 1997. Its briefly occur by Incubattion approximately 5 ml of liquid culture media with bacteria at optimum condition of growth for 24 hr. transfer 1.5ml of cu ...
Polymerase chain reaction and its applications
... TaqMan assays exploit the fact that Taq polymerase possesses a 50 --30 exonuclease activity in addition to its 50 -30 -polymerase activity.TaqMan PCR differs from conventional PCR by the addition of a third oligonucleotide, called the TaqMan probe, to the PCR reaction. The TaqMan probe is a dually l ...
... TaqMan assays exploit the fact that Taq polymerase possesses a 50 --30 exonuclease activity in addition to its 50 -30 -polymerase activity.TaqMan PCR differs from conventional PCR by the addition of a third oligonucleotide, called the TaqMan probe, to the PCR reaction. The TaqMan probe is a dually l ...
Article A Molecular Evolutionary Reference for the Human Variome
... we present a new molecular evolutionary method for estimating neutral evolutionary probabilities (EPs) of each amino acid, or nucleotide state at a genomic position without using intraspecific polymorphism data. Because EPs are derived independently of population-level information, they serve as nul ...
... we present a new molecular evolutionary method for estimating neutral evolutionary probabilities (EPs) of each amino acid, or nucleotide state at a genomic position without using intraspecific polymorphism data. Because EPs are derived independently of population-level information, they serve as nul ...
Unit F212/01 - Molecules, biodiversity, food and health
... on the rate of pepsin activity over the whole range of substrate concentrations. ..................................... This should be answered on Fig. 1.1 ..................................... [2] ...
... on the rate of pepsin activity over the whole range of substrate concentrations. ..................................... This should be answered on Fig. 1.1 ..................................... [2] ...
Electrokinetic Stretching of Tethered DNA
... Shaqfeh, 2003). The observation of single molecules of DNA using fluorescence microscopy has also provided new information on biological processes. For example, several groups have investigated the structure of chromatin fibers, a packaging structure in eukaryotes made up of DNA wound tightly around ...
... Shaqfeh, 2003). The observation of single molecules of DNA using fluorescence microscopy has also provided new information on biological processes. For example, several groups have investigated the structure of chromatin fibers, a packaging structure in eukaryotes made up of DNA wound tightly around ...
Package `rDNA`
... If the attenuation algorithm is used, lambda provides the decay constant for the exponential decay function. The default value of 0.1 attributes relatively high weight to statements which are made within approximately five to ten days. ignore.agreement This argument is only used if algorithm="attenu ...
... If the attenuation algorithm is used, lambda provides the decay constant for the exponential decay function. The default value of 0.1 attributes relatively high weight to statements which are made within approximately five to ten days. ignore.agreement This argument is only used if algorithm="attenu ...
Kit Manual - CR Scientific
... 250 μL, add appropriate volume of PBS to 250 μL. For samples larger than 250 μL, split each sample into two 250 μL aliquots and use two wells of the 1.2 mL round well plate for lysis. Load the combined lysates into each well of the 96-Well DNA Plate. ...
... 250 μL, add appropriate volume of PBS to 250 μL. For samples larger than 250 μL, split each sample into two 250 μL aliquots and use two wells of the 1.2 mL round well plate for lysis. Load the combined lysates into each well of the 96-Well DNA Plate. ...
Table 7. Summary statistics for the consensus gene set of Haliotis
... discus hannai was 1.86 Gb, and this is the biggest genome among known gastropods. It is 5.31 and 2.02 times larger than genomes size of L.gigantea (0.35 Gb) and A.californica (0.92 Gb) in the same Gastropoda class. In animals, the increase of genome size is commonly driven by transposable element, ...
... discus hannai was 1.86 Gb, and this is the biggest genome among known gastropods. It is 5.31 and 2.02 times larger than genomes size of L.gigantea (0.35 Gb) and A.californica (0.92 Gb) in the same Gastropoda class. In animals, the increase of genome size is commonly driven by transposable element, ...
EVOLUTION: Unifying Concept in Biology
... North American invasive populations are triploid and sterile, reproduce asexually Taraxacum officinale ...
... North American invasive populations are triploid and sterile, reproduce asexually Taraxacum officinale ...
Non contiguous-finished genome sequence and description of
... Phylogenetic tree highlighting the position of Bacillus jeddahensis strain JCET relative to other type strains within the Bacillus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using MUSCLE, and phylogenetic inferences obtained using the maximum-likelihood met ...
... Phylogenetic tree highlighting the position of Bacillus jeddahensis strain JCET relative to other type strains within the Bacillus genus. GenBank accession numbers are indicated in parentheses. Sequences were aligned using MUSCLE, and phylogenetic inferences obtained using the maximum-likelihood met ...
Molecular and Immunological Methods
... target amplicon. The simplest approach relies upon determining the melting temperature of the amplicon using a melting curve. The real time PCR is performed as normal, incorporating a non-hydrolysed probe or dye – typically performed with SYBR Green or a saturation dye such as SYTO 9 or LC Green 1. ...
... target amplicon. The simplest approach relies upon determining the melting temperature of the amplicon using a melting curve. The real time PCR is performed as normal, incorporating a non-hydrolysed probe or dye – typically performed with SYBR Green or a saturation dye such as SYTO 9 or LC Green 1. ...
Assembly of complete KIR haplotypes from a diploid individual
... unambiguously assemble individual haplotypes for the highly repetitive 100-200 kb killer Ig-like receptor (KIR) gene loci of chromosome 19. A tiling of targeted fosmids can be used to clone extended lengths of genomic DNA, 100s of kb in length, but repeat complexity in regions of particular interest ...
... unambiguously assemble individual haplotypes for the highly repetitive 100-200 kb killer Ig-like receptor (KIR) gene loci of chromosome 19. A tiling of targeted fosmids can be used to clone extended lengths of genomic DNA, 100s of kb in length, but repeat complexity in regions of particular interest ...
PDF
... a meta-language designed for syntactic descriptions of language! that was originally devised to define ALGOL 60 (4,9,6). The language we describe, which we call DNA*, differs in some ways from what was used in the file splicing program that inspired it. A most important difference is that DNA* emplo ...
... a meta-language designed for syntactic descriptions of language! that was originally devised to define ALGOL 60 (4,9,6). The language we describe, which we call DNA*, differs in some ways from what was used in the file splicing program that inspired it. A most important difference is that DNA* emplo ...
Student`s guide -
... Individuals who are homozygous for the dominant allele (DD) only have DNA of type D, which is not cut by the restriction enzyme. Gel electrophoresis separates DNA fragments by size, so in samples with just the D allele, all of the uncut fragments of DNA will move together to form a single band on th ...
... Individuals who are homozygous for the dominant allele (DD) only have DNA of type D, which is not cut by the restriction enzyme. Gel electrophoresis separates DNA fragments by size, so in samples with just the D allele, all of the uncut fragments of DNA will move together to form a single band on th ...
Mgr. Martina Višňovská Alignments on Sequences with Internal
... the whole query Q, as each read has to map completely to the reference. We will say that the minimum Hamming distance between Q and some substring of D with length m is the distance of Q and D. We will represent a similarity score between Q and an m-tuple from D by the substraction m minus the Hammi ...
... the whole query Q, as each read has to map completely to the reference. We will say that the minimum Hamming distance between Q and some substring of D with length m is the distance of Q and D. We will represent a similarity score between Q and an m-tuple from D by the substraction m minus the Hammi ...
New Perspectives on Rickettsial Evolution from New
... because of its similar obligate intracellular growth in the cytoplasm of infected host cells, in 1995 it was reclassified from Rickettsia to the genus Orientia due to its clear phenotypic and genotypic differences from that genus.22 These traits included its different cell wall structure and apparen ...
... because of its similar obligate intracellular growth in the cytoplasm of infected host cells, in 1995 it was reclassified from Rickettsia to the genus Orientia due to its clear phenotypic and genotypic differences from that genus.22 These traits included its different cell wall structure and apparen ...
Cloning and Sequencing of a Gene from Bacillus
... primary sequences derived from the nucleotide sequences of the two genes were also compared. The gene from B. amyloliquefaciens coded for a protein of 344 amino acid residues, one more than the protein coded by the corresponding gene from B. subtilis. Comparison of the primary amino acid sequences o ...
... primary sequences derived from the nucleotide sequences of the two genes were also compared. The gene from B. amyloliquefaciens coded for a protein of 344 amino acid residues, one more than the protein coded by the corresponding gene from B. subtilis. Comparison of the primary amino acid sequences o ...