Specific oligonucleotide primers for detection of endoglucanase
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
... endoglucanase gene of B. subtilis, G. stearothermophilus and P. campinasensis. Two PCR primers, EN1F and EN1R, were chosen that were predicted to specifically amplify a 1,311 bpDNA fragment of the B. Subtilis, G. stearothermophilus and P. campinasensis. The Genbank database (NCBI) search for complim ...
systematics of moonworts botrychium subgenus
... Kato (1987) split Osmundopteris into two subgenera, Botrypus (containing B. virginianum) and Japanobotrychium. Using two molecular data sets plus morphological/anatomical characters, Hauk (2000) reported Botrychium and Sceptridium to be well supported entities, but found Botrypus to be paraphyletic. ...
... Kato (1987) split Osmundopteris into two subgenera, Botrypus (containing B. virginianum) and Japanobotrychium. Using two molecular data sets plus morphological/anatomical characters, Hauk (2000) reported Botrychium and Sceptridium to be well supported entities, but found Botrypus to be paraphyletic. ...
Improvement of DNA Extraction Protocols for Nostochopsis spp.
... kits has been successful in many plants with mucilaginous algae [8, 9]. However, this method has not worked with mucilaginous cyanobacteria, and particularly Nostochopsis. The lysis buffer and lysis condition in those kits are not sufficient to obtain good quality and a high quantity of DNA from the ...
... kits has been successful in many plants with mucilaginous algae [8, 9]. However, this method has not worked with mucilaginous cyanobacteria, and particularly Nostochopsis. The lysis buffer and lysis condition in those kits are not sufficient to obtain good quality and a high quantity of DNA from the ...
Studies on Chlamydomonas Chloroplast Transformation: Foreign
... of nonphotosynthetic, acetate-requiring mutants of Chlamydomonas reinhardtii with genetic lesions in the chloroplast-encoded atpB, psbA, and rbcL genes by wild-type DNA sequences delivered on microprojectiles. DNA gel blot analysis of photosynthetically active transformants obtained from an atpB del ...
... of nonphotosynthetic, acetate-requiring mutants of Chlamydomonas reinhardtii with genetic lesions in the chloroplast-encoded atpB, psbA, and rbcL genes by wild-type DNA sequences delivered on microprojectiles. DNA gel blot analysis of photosynthetically active transformants obtained from an atpB del ...
Sequence Alignment
... Think about aligning 2 proteins from similar species that are orthologs: same function and syntenic. At some point back in evolutionary time, there was a single DNA sequence that is the common ancestor of both proteins. – Most paired amino acids are identical, but a few are different. Reduce the pro ...
... Think about aligning 2 proteins from similar species that are orthologs: same function and syntenic. At some point back in evolutionary time, there was a single DNA sequence that is the common ancestor of both proteins. – Most paired amino acids are identical, but a few are different. Reduce the pro ...
Proceeding Paper - American Meat Science Association
... respectively. The genus Prevotella was the most abundant taxon and ranged from 7 to 40% of the sequences and 45 genera were observed across the 6 animals. The genus Prevotella was least variable at 5.7-fold difference, whereas the genus Anaerostipes was the most diverse at over 14-fold difference ac ...
... respectively. The genus Prevotella was the most abundant taxon and ranged from 7 to 40% of the sequences and 45 genera were observed across the 6 animals. The genus Prevotella was least variable at 5.7-fold difference, whereas the genus Anaerostipes was the most diverse at over 14-fold difference ac ...
Week 8 Lab: SNP Detection, SNP Discussion
... A host shift refers to movement of a parasitic species from one host to a new species of host (in our case, from one species of goldenrod to the other). A host race is a group of organisms (e.g. gall flies) which is found on one type of host (e.g. S. altissima); the term does not go so far as to imp ...
... A host shift refers to movement of a parasitic species from one host to a new species of host (in our case, from one species of goldenrod to the other). A host race is a group of organisms (e.g. gall flies) which is found on one type of host (e.g. S. altissima); the term does not go so far as to imp ...
Polymerase Chain Reaction (PCR) - Department of Environmental
... combined into one tube, and then dispensed among the individual reaction tubes. Template DNA is then added to each tube. This limits pipetting errors as well as the potential for contamination, as the reagent tubes are opened and dispensed from only one time per reaction set-up. Notice also that the ...
... combined into one tube, and then dispensed among the individual reaction tubes. Template DNA is then added to each tube. This limits pipetting errors as well as the potential for contamination, as the reagent tubes are opened and dispensed from only one time per reaction set-up. Notice also that the ...
Recovery of DNA for Forensic Analysis from Lip Cosmetics*
... while many of the extracts were pigmented, not all such extracts resulted in fluorescent artefacts occurring in the DNA profile, and artifacts in the DNA profiles were also observed from extracts that appeared colorless. The Blue and Green wavelengths were the most commonly affected, but artifacts w ...
... while many of the extracts were pigmented, not all such extracts resulted in fluorescent artefacts occurring in the DNA profile, and artifacts in the DNA profiles were also observed from extracts that appeared colorless. The Blue and Green wavelengths were the most commonly affected, but artifacts w ...
Associative and Endophytic Nitrogen-fixing Bacteria and Cyanobacterial Association
... physiologically active cells with rRNA-targeting fluorescence labeled probes. For a first screening of bacterial isolates from any plant environment, a large set of phylogenetic oligonucleotide probes (Table 1) can be used in a hierarchical manner from the kingdom down to the genus and species level ...
... physiologically active cells with rRNA-targeting fluorescence labeled probes. For a first screening of bacterial isolates from any plant environment, a large set of phylogenetic oligonucleotide probes (Table 1) can be used in a hierarchical manner from the kingdom down to the genus and species level ...
Genetic Diversity in the Paramecium aurelia Species Complex
... hand, a number of small-scale polymorphism surveys of protein-coding loci in free-living microbes, freshwater ciliates in particular, have produced conflicting estimates of intraspecific variation (Gerber et al. 2002; Barth et al. 2006; Katz et al. 2006; Snoke et al. 2006), thus impeding wider concl ...
... hand, a number of small-scale polymorphism surveys of protein-coding loci in free-living microbes, freshwater ciliates in particular, have produced conflicting estimates of intraspecific variation (Gerber et al. 2002; Barth et al. 2006; Katz et al. 2006; Snoke et al. 2006), thus impeding wider concl ...
(Brassica tournefortii) Invasion in North American Deserts
... the tiny, stiff urticating hairs on stems and broad leaves. Conversely, many desert natives are browsed by both vertebrates and insects, including voracious caterpillars during periodic mass irruption cycles. As a consequence, type conversion of native flora to a monoculture of Sahara mustard result ...
... the tiny, stiff urticating hairs on stems and broad leaves. Conversely, many desert natives are browsed by both vertebrates and insects, including voracious caterpillars during periodic mass irruption cycles. As a consequence, type conversion of native flora to a monoculture of Sahara mustard result ...
Section Title – One Line Preferred, Two Line Maximum
... One PCR cycle consists of a DNA denaturation step, a primer annealing step and a primer extension step. DNA Denaturation: Expose the DNA template to high temperatures to separate the two DNA strands and allow access by DNA polymerase and PCR primers. Primer Annealing: Lower the temperature to allow ...
... One PCR cycle consists of a DNA denaturation step, a primer annealing step and a primer extension step. DNA Denaturation: Expose the DNA template to high temperatures to separate the two DNA strands and allow access by DNA polymerase and PCR primers. Primer Annealing: Lower the temperature to allow ...
Complex patterns of hybridization between exotic and - UvA-DARE
... between native and exotic species relatively easy (though this depends on their relatedness). Furthermore, we know that at least some of the hybrids are able to survive to sexual maturity and successfully backcross with the parental species or with other poplar species (Thompson et al., 2010). In fa ...
... between native and exotic species relatively easy (though this depends on their relatedness). Furthermore, we know that at least some of the hybrids are able to survive to sexual maturity and successfully backcross with the parental species or with other poplar species (Thompson et al., 2010). In fa ...
Biochemistry - Stryer - Science and Technology
... sequence complete genomes: first, small genomes from viruses; then, larger genomes from bacteria; and, finally, eukaryotic genomes, including the 3-billion-base-pair human genome. Scientists are just beginning to exploit the enormous information content of these genome sequences. Finally, recombinan ...
... sequence complete genomes: first, small genomes from viruses; then, larger genomes from bacteria; and, finally, eukaryotic genomes, including the 3-billion-base-pair human genome. Scientists are just beginning to exploit the enormous information content of these genome sequences. Finally, recombinan ...
Comparison of Target-Capture and Restriction
... Associate Editor: John McCormack Abstract.—Restriction-site associated DNA sequencing (RAD-seq) and target capture of specific genomic regions, such as ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reducedrepresentation genomic data sets. Thes ...
... Associate Editor: John McCormack Abstract.—Restriction-site associated DNA sequencing (RAD-seq) and target capture of specific genomic regions, such as ultraconserved elements (UCEs), are emerging as two of the most popular methods for phylogenomics using reducedrepresentation genomic data sets. Thes ...
90718 Internal v2 3.6 A2 Generic 2006
... The plasmids containing the different fragments are cultured to produce many copies. The production of a clone library is necessary, as the amount of material in a genome is so large it is impossible to deal with all at once. Using a clone library also means that each of the cultures containing a fr ...
... The plasmids containing the different fragments are cultured to produce many copies. The production of a clone library is necessary, as the amount of material in a genome is so large it is impossible to deal with all at once. Using a clone library also means that each of the cultures containing a fr ...
Chapter 13
... 13.1 What Is the Evidence that the Gene Is DNA? 13.2 What Is the Structure of DNA? ...
... 13.1 What Is the Evidence that the Gene Is DNA? 13.2 What Is the Structure of DNA? ...
Investigating a Eukaryotic Genome
... search. From the search results, students determine the identity of the cloned fragment. Approximately 50% of the yeast genome is estimated to contain genetic information, so not all students clone a gene. Many students will instead clone a segment of DNA that contains control elements or repetitive ...
... search. From the search results, students determine the identity of the cloned fragment. Approximately 50% of the yeast genome is estimated to contain genetic information, so not all students clone a gene. Many students will instead clone a segment of DNA that contains control elements or repetitive ...
An improved technique for isolating codominant compound
... individual trees (Table 1). Of these 13 primer pairs, 12 produced one or two bands, and one produced multiple bands. The former 12 primer pairs contained one producing a monomorphic band for all individuals. The other 11 primer pairs showed polymorphism ranging from 4 to 12 alleles per locus. The ob ...
... individual trees (Table 1). Of these 13 primer pairs, 12 produced one or two bands, and one produced multiple bands. The former 12 primer pairs contained one producing a monomorphic band for all individuals. The other 11 primer pairs showed polymorphism ranging from 4 to 12 alleles per locus. The ob ...
Lec 19
... constitute duplex DNA molecules are sufficiently rigid to be very easily broken by shear forces in solution. In this method, high molecular weight DNA is sheared to population of molecules with a mean size of about 8kb pairs by stirring at 1500 rpm for 30 minutes. Breakage occurs essentially at rand ...
... constitute duplex DNA molecules are sufficiently rigid to be very easily broken by shear forces in solution. In this method, high molecular weight DNA is sheared to population of molecules with a mean size of about 8kb pairs by stirring at 1500 rpm for 30 minutes. Breakage occurs essentially at rand ...
Chapter 6 – Exam style questions Q1. Bk Ch6 Exam MQ1 What is
... Comparative embryology of the vertebrates shown clearly indicates similarities between the different organisms in the early stages of development. Such similarities suggest an evolutionary relationship between the organisms such that at some point in the history of their development they shared a co ...
... Comparative embryology of the vertebrates shown clearly indicates similarities between the different organisms in the early stages of development. Such similarities suggest an evolutionary relationship between the organisms such that at some point in the history of their development they shared a co ...
Ever since the days of Rene Descartes, the French philosopher
... characterised five years later. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzyme ...
... characterised five years later. It was found that Hind II always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs. This specific base sequence is known as the recognition sequence for Hind II. Besides Hind II, today we know more than 900 restriction enzyme ...