Modification of the terminal restriction fragment length polymorphism
... taxa of interest. The detected TRF may be assigned to a taxonomic group or species provided its 16S rDNA sequence is available. However even for unknown species, the method yields an information on their presence and TRF sizes, which may be used to clone the respective fragments and thus to identify ...
... taxa of interest. The detected TRF may be assigned to a taxonomic group or species provided its 16S rDNA sequence is available. However even for unknown species, the method yields an information on their presence and TRF sizes, which may be used to clone the respective fragments and thus to identify ...
1-2 Teacher
... have double or triple the normal number of chromosomes. This condition, known as polyploidy, produces new species of plants that are often larger and stronger than their diploid relatives. Polyploidy in animals is usually fatal. Except in the case of the Red Viscacha Rat Slide 12 of 18 Copyright Pea ...
... have double or triple the normal number of chromosomes. This condition, known as polyploidy, produces new species of plants that are often larger and stronger than their diploid relatives. Polyploidy in animals is usually fatal. Except in the case of the Red Viscacha Rat Slide 12 of 18 Copyright Pea ...
On the use of genetic divergence for identifying
... Systematists attempt to describe the variation among taxa as well as the historical relationships among them. The rules used in performing this task can be complex: e.g. Johns & Avise (1998) suggested that the criteria for identifying generic and familial level distinctions in birds appear to differ ...
... Systematists attempt to describe the variation among taxa as well as the historical relationships among them. The rules used in performing this task can be complex: e.g. Johns & Avise (1998) suggested that the criteria for identifying generic and familial level distinctions in birds appear to differ ...
Contig annotation tool CAT robustly classifies assembled
... in detail. In the example shown in Figure 2, the DIAMOND homology searches of the protein translations of five ORFs identified on the DNA sequence yielded four, zero, three, four, and four hits, respectively. For the first ORF, the top DIAMOND hit is present in Escherichia coli and the hit had a bit ...
... in detail. In the example shown in Figure 2, the DIAMOND homology searches of the protein translations of five ORFs identified on the DNA sequence yielded four, zero, three, four, and four hits, respectively. For the first ORF, the top DIAMOND hit is present in Escherichia coli and the hit had a bit ...
Lab 6: Electrophoresis
... between identical DNA sequences within the chromosome. Some restriction enzymes cut cleanly through the DNA helix at the same position on both strands to produce fragments with blunt ends (Figure 1). Other endonucleses cleave each strand off-center at specific nucleotides to produce fragments with “ ...
... between identical DNA sequences within the chromosome. Some restriction enzymes cut cleanly through the DNA helix at the same position on both strands to produce fragments with blunt ends (Figure 1). Other endonucleses cleave each strand off-center at specific nucleotides to produce fragments with “ ...
Mitochondrial DNA disease - Human Molecular Genetics
... exposure to ambient conditions (29). Therefore, it will be important to perform the manipulations in tightly controlled environmental conditions and to develop techniques to minimize the risk of inducing aneuploidy during transplantation of the metaphase II spindle. While pronuclear transfer offers ...
... exposure to ambient conditions (29). Therefore, it will be important to perform the manipulations in tightly controlled environmental conditions and to develop techniques to minimize the risk of inducing aneuploidy during transplantation of the metaphase II spindle. While pronuclear transfer offers ...
Determination of DNA Melting Temperatures in Diffusion
... For fast and reliable determination of DNA melting temperatures with single-nucleotide resolution in a microfluidic setup, stable gradients of the denaturing agent formamide were generated by means of diffusion. Formamide lowers the melting temperature of DNA, and a given formamide concentration can ...
... For fast and reliable determination of DNA melting temperatures with single-nucleotide resolution in a microfluidic setup, stable gradients of the denaturing agent formamide were generated by means of diffusion. Formamide lowers the melting temperature of DNA, and a given formamide concentration can ...
Sequencing genomes
... • BLASTP – protein sequences • BLASTX – uses nucleotide sequences as queries and translates them in all six reading frames to produce translated protein sequences, which are used to query a protein sequence database • TBLASTN – queries protein sequences to a nucleotide sequence database with the seq ...
... • BLASTP – protein sequences • BLASTX – uses nucleotide sequences as queries and translates them in all six reading frames to produce translated protein sequences, which are used to query a protein sequence database • TBLASTN – queries protein sequences to a nucleotide sequence database with the seq ...
Sequencing genomes
... • BLASTP – protein sequences • BLASTX – uses nucleotide sequences as queries and translates them in all six reading frames to produce translated protein sequences, which are used to query a protein sequence database • TBLASTN – queries protein sequences to a nucleotide sequence database with the seq ...
... • BLASTP – protein sequences • BLASTX – uses nucleotide sequences as queries and translates them in all six reading frames to produce translated protein sequences, which are used to query a protein sequence database • TBLASTN – queries protein sequences to a nucleotide sequence database with the seq ...
Salmonella typhimurium
... a. Gene ID Systems This Salmonella typhimurium Gene Database is UniProt-centric in that the main data source (primary ID System) for gene IDs and annotation is the UniProt complete proteome set for Salmonella typhimurium, made available as an XML download by the Integr8 resource. In addition to UniP ...
... a. Gene ID Systems This Salmonella typhimurium Gene Database is UniProt-centric in that the main data source (primary ID System) for gene IDs and annotation is the UniProt complete proteome set for Salmonella typhimurium, made available as an XML download by the Integr8 resource. In addition to UniP ...
AUTOMATED DNA SEQUENCING, MegaBACE 1000
... Modern versions of Sanger’s original technique generate fluorescently labeled DNA fragments which are read in an automated fashion with electronic data capture. All possible fragment sizes from 25 base pairs up to 1000 bases are produced by the sequencing chemistry with each fragment containing a s ...
... Modern versions of Sanger’s original technique generate fluorescently labeled DNA fragments which are read in an automated fashion with electronic data capture. All possible fragment sizes from 25 base pairs up to 1000 bases are produced by the sequencing chemistry with each fragment containing a s ...
Transposable Elements in Rice Plants
... Distribution and activity of Ac/ Os-like elements in the genome The low stringent hybridi zation or the rice genomes wi th the RAc probe showed that Ac-like elements were widely distributed in the genus Oryza including wild and cultivated rice species. One example or the hybridization experiments wa ...
... Distribution and activity of Ac/ Os-like elements in the genome The low stringent hybridi zation or the rice genomes wi th the RAc probe showed that Ac-like elements were widely distributed in the genus Oryza including wild and cultivated rice species. One example or the hybridization experiments wa ...
Molecular Evidence for Vector Implication of Onchocerca lupi in Los
... Onchocerca is a genus of filarial parasites with worldwide distribution and is typically associated with ungulates, including horses and cattle. The causative agent of human “river blindness” also resides within the same genus (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). Historically, canids wer ...
... Onchocerca is a genus of filarial parasites with worldwide distribution and is typically associated with ungulates, including horses and cattle. The causative agent of human “river blindness” also resides within the same genus (Zarfoss, Dubielzig, Eberhard, & Schmidt, 2005). Historically, canids wer ...
Chromosomes and DNA Replication
... 46 chromosomes per cell. Each chromosome in one set is matched by a chromosome of the same type in the other set, so there are actually 23 pairs of chromosomes per cell. Each pair consists of chromosomes of the same size and shape that also contain the same genes. The chromosomes in a pair are known ...
... 46 chromosomes per cell. Each chromosome in one set is matched by a chromosome of the same type in the other set, so there are actually 23 pairs of chromosomes per cell. Each pair consists of chromosomes of the same size and shape that also contain the same genes. The chromosomes in a pair are known ...
HiPer® Real-Time PCR Teaching Kit
... ‘real time’ instead of detecting the end product at the end. For this reason it is called quantitative PCR which identifies the products using either a fluorescent dye or a probe. ...
... ‘real time’ instead of detecting the end product at the end. For this reason it is called quantitative PCR which identifies the products using either a fluorescent dye or a probe. ...
Molecular biology technique (I) Southern/Northern
... • Add 10 l formaldehyde loading buffer to each sample and load gel. Run gel at 100 to 120 V for ~3hr. • Remove gel from the running tank and rinse several times in water. Place gel in 10x SSC for ...
... • Add 10 l formaldehyde loading buffer to each sample and load gel. Run gel at 100 to 120 V for ~3hr. • Remove gel from the running tank and rinse several times in water. Place gel in 10x SSC for ...
Analysis of 16S ribosomal RNA gene segments for the diagnosis of
... sequence library. The process has been done by using broad-range PCR primers (universal primers) to recognize conserved sequences in a variety of bacteria, while amplifying highly variable regions between the primer binding sites. The amplified segment is sequenced and compared with known databases ...
... sequence library. The process has been done by using broad-range PCR primers (universal primers) to recognize conserved sequences in a variety of bacteria, while amplifying highly variable regions between the primer binding sites. The amplified segment is sequenced and compared with known databases ...
lecture03_08
... Complexity for genomes • Human genome contains 3 109 base pairs – Searching an mRNA against HG requires ~1013 cells -Even efficient exact algorithms will be extremely slow when preformed millions of times. -Running the computations in parallel is expensive. ...
... Complexity for genomes • Human genome contains 3 109 base pairs – Searching an mRNA against HG requires ~1013 cells -Even efficient exact algorithms will be extremely slow when preformed millions of times. -Running the computations in parallel is expensive. ...
Chapter 6: Cell Growth and Reproduction Lesson 6.2
... 46 chromosomes per cell. Each chromosome in one set is matched by a chromosome of the same type in the other set, so there are actually 23 pairs of chromosomes per cell. Each pair consists of chromosomes of the same size and shape that also contain the same genes. The chromosomes in a pair are known ...
... 46 chromosomes per cell. Each chromosome in one set is matched by a chromosome of the same type in the other set, so there are actually 23 pairs of chromosomes per cell. Each pair consists of chromosomes of the same size and shape that also contain the same genes. The chromosomes in a pair are known ...
How Should Species Phylogenies Be Inferred from
... should never be combined (the “separate analysis” approach). Intermediate between these two extremes is the “prior agreement” method espoused by some (e.g., Bull et al., 1993), which argues that data sets should be combined only if not significantly heterogeneous. In the sense that we espouse combin ...
... should never be combined (the “separate analysis” approach). Intermediate between these two extremes is the “prior agreement” method espoused by some (e.g., Bull et al., 1993), which argues that data sets should be combined only if not significantly heterogeneous. In the sense that we espouse combin ...
Newsletter 1
... common male ancestor. A similar test for a common female ancestor is also available using ‘mitochondrial’ DNA, but as names in our society pass down the male line, this is less useful for many family history purposes. Specific parts of the Y-chromosome known as ‘markers’ are used to make such compar ...
... common male ancestor. A similar test for a common female ancestor is also available using ‘mitochondrial’ DNA, but as names in our society pass down the male line, this is less useful for many family history purposes. Specific parts of the Y-chromosome known as ‘markers’ are used to make such compar ...