Download Electrophoresis, Blotting and Immunodetection Gel

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Transcript
Electrophoresis, Blotting and Immunodetection
Gel extraction kits
Gel storage system, GelFile™
03
•
Stores, organises and displays dried polyacrylamide and agarose gels
•
Each BioDesign GelFile™ binder contains heavy crystal clear vinyl pages with pockets and white backgrounds
•
The pages are chemically neutral and are guaranteed not to alter your dried gels or turn yellow for TEN years
•
Dried gels are held flat for long term storage without curling
•
Shields gels from moisture, accidental spills and possible damage
•
White background allows optimal comparison between gels
•
Each BioDesign GelFile™ binder holds 20 to 25 pages
•
A clear label holder on the spine allows you to personalise each binder
Catalogue No
ELE-955-070N
Alt. No
G301
Description
GelFile™ for gels up to 115mm x 180mm, includes
folder and 20 sheets
Pack qty
1
Price
51.39
ELE-955-090H
G303
GelFile™ for gels up to 180mm x 255mm, includes
folder and 20 sheets
1
51.39
ELE-955-110E
ELE-955-130V
ELE-955-150P
ELE-955-170J
ELE-955-190D
G305
G307
G309
G311
G313
GelFile™ binder
Gel protector sheets, for gels up to 115mm x 180mm
Gel protector sheets, for gels up to 115mm x 180mm
Gel protector sheets, for gels up to 180mm x 255mm
Gel protector sheets, for gels up to 180mm x 255mm
1
20
40
20
40
12.19
42.68
78.39
42.68
78.39
Gel extraction kit, Montage
71
For eluting DNA from agarose gels.
•
Prepares gel-purified PCR* products for sequencing or cloning without need for further purification
•
Elutes DNA from agarose gels in 10min
•
Simple protocol with minimum hands-on time
•
DNA of 100 to 10,000bp from standard agarose gels is typically greater than 70%
Extract DNA from agarose gels in a single 10min spin with this easy-to-use kit.
A gel nebuliser converts agarose to a spray from which DNA is separated (via a 0.45µm Ultrafree-MC filter). Prepares
gel-purified PCR products for sequencing or cloning without need for further purification. Device volume is 100µL of gel.
Typically recovers >70% of 100 to 10,000bp DNA from standard agarose gels.
Kit components: 50 gel nebulisers; 50 Ultrafree-MC 0.45µm separators; 50 microcentrifuge vials; 500mL stock of 50x
modified TAE buffer; and a protocol.
Catalogue No
FDR-578-011V
Alt. No
LSKGEL050
Description
Gel extraction kit
Sample volume
100µL of gel
Pack qty
50
Price
89.03
*Polymerase Chain Reaction (PCR) is a process covered by
patents owned by Hoffman-La Roche
Ultra agarose spin kit, Thermo Scientific ABgene
LD
•
Ultra fast protocol - DNA is ready to use in 5 to 6
minutes
•
Low melting point agarose not required
The Thermo Scientific ABgene Ultra agarose spin kit is
an advanced system for isolating DNA from agarose gels
using spin filters. The purified DNA can be used directly
for any downstream application.
•
Can be used for TAE and TBE gels
Components
•
No specialised equipment required
•
60mL binding buffer
•
DNA is eluted into sterile water or elution buffer
(supplied)
•
30mL wash solution
•
5mL elution buffer
•
100 silica spin filters
•
100 x 2mL collection tubes
•
No precipitation required
•
DNA size range: 100bp to 50kb
•
Binding capacity of spin filter: up to 20µg
•
Recovery rate: 80% to 100% (depending on gel type
and fragment size)
Catalogue No
PCR-725-010U
Alt. No
AB-0842
Storage: Stable for 1 year at room temperature. The kit is
shipped at ambient temperature.
Preps per kit
100 silica spin filters, buffers and tubes
Pack qty
1
Price
175.45
871