bacteriophages
... production, and most of the serological work with phage has been done with rabbit sera. The phages themselves are nontoxic and nonpathogenic for animals and can usually be injected in large amounts without damage to the recipient. Crude phage lysates, however, may contain toxic bacterial substances, ...
... production, and most of the serological work with phage has been done with rabbit sera. The phages themselves are nontoxic and nonpathogenic for animals and can usually be injected in large amounts without damage to the recipient. Crude phage lysates, however, may contain toxic bacterial substances, ...
A monoclonal antibody against a carbohydrate
... The genes encoding the variable domains of mAb S69-4 were cloned and expressed as single-chain variable fragments (scFv) to allow a sequence comparison with the two well characterized antibodies mAb S45-18 and mAb S25-2 which have been crystallized in complex with the antigen (Nguyen et al., 2003). ...
... The genes encoding the variable domains of mAb S69-4 were cloned and expressed as single-chain variable fragments (scFv) to allow a sequence comparison with the two well characterized antibodies mAb S45-18 and mAb S25-2 which have been crystallized in complex with the antigen (Nguyen et al., 2003). ...
Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... makes it attractive for mAb purification (Linhult et al., 2004). While, the purification of target proteins fused to ELPs has been previously demonstrated (Meyer and Chilkoti, 1999), this approach typically requires proteolytic cleavage of the target protein from the ELP prior to final recovery. Banki ...
... makes it attractive for mAb purification (Linhult et al., 2004). While, the purification of target proteins fused to ELPs has been previously demonstrated (Meyer and Chilkoti, 1999), this approach typically requires proteolytic cleavage of the target protein from the ELP prior to final recovery. Banki ...
3D structure of the CD26–ADA complex obtained - diss.fu
... multiple cloning site of pFastBacHTc (Gibco-BRL) vector for expression in Sf9 insect cells. Recombinant bacmid-DNA and high-titer baculovirus stock were generated and human CD26 was expressed in Sf9 cells as described earlier [37]. Specific rabbit anti-CD26 IgGs were separated from polyclonal antiser ...
... multiple cloning site of pFastBacHTc (Gibco-BRL) vector for expression in Sf9 insect cells. Recombinant bacmid-DNA and high-titer baculovirus stock were generated and human CD26 was expressed in Sf9 cells as described earlier [37]. Specific rabbit anti-CD26 IgGs were separated from polyclonal antiser ...
IMMUNOREACTIONS INVOLVING PLATELETS. VI. REACTIONS OF
... which differs (see below) from PlAl. The antiglobulin consumption test, using Mother K's serum alone, was consistently but weakly positive with PlA(1,1) platelets, but negative with PlA(-,-) platelets. The amount of antiglobulin consumed by PlA(1,1) platelets exposed to Mother K's serum was similar ...
... which differs (see below) from PlAl. The antiglobulin consumption test, using Mother K's serum alone, was consistently but weakly positive with PlA(1,1) platelets, but negative with PlA(-,-) platelets. The amount of antiglobulin consumed by PlA(1,1) platelets exposed to Mother K's serum was similar ...
MDM2 inhibit p300-mediated p53 acetylation and activation by
... Whether the inhibition requires the association of MDM2 with p53 compare wild-type MDM2 with its N-terminally deleted form lacking amino acid 58-89 (p53 binding domain) ...
... Whether the inhibition requires the association of MDM2 with p53 compare wild-type MDM2 with its N-terminally deleted form lacking amino acid 58-89 (p53 binding domain) ...
Monoclonal Antibodies Specific for Oxidative Phosphorylation
... proteins involved in the oxidative phosphorylation system are integral membrane proteins, which contain a large number of hydrophobic amino acids that are likely to cause the protein to run aberrantly, with respect to their molecular weight, in SDS polyacrylamide gel electrophoresis. ...
... proteins involved in the oxidative phosphorylation system are integral membrane proteins, which contain a large number of hydrophobic amino acids that are likely to cause the protein to run aberrantly, with respect to their molecular weight, in SDS polyacrylamide gel electrophoresis. ...
KingFisher Pure DNA Plant Kit
... Thermo Scientific™ KingFisher™ Flex with a 96 deep well head or the Thermo Scientific™ KingFisher™ Duo with a 12-pin head and a sample amount of up to 50 mg. The user will need the KingFisher Flex or KingFisher Duo magnetic particle processor for conducting purification (Table 1-2). In addition, sev ...
... Thermo Scientific™ KingFisher™ Flex with a 96 deep well head or the Thermo Scientific™ KingFisher™ Duo with a 12-pin head and a sample amount of up to 50 mg. The user will need the KingFisher Flex or KingFisher Duo magnetic particle processor for conducting purification (Table 1-2). In addition, sev ...
STUDIES ON THE IMMOBILIZATION ANTIGENS OF PARAMECIUM
... Tests for the action of proteolytic enzymes were made by exposing either crude extract or purified antigen to the enzymes. After a suitable incubation period, the mixture was added to antiserum, and then paramecia were added to see if the antigen had absorbed antibody from the serum or whether its c ...
... Tests for the action of proteolytic enzymes were made by exposing either crude extract or purified antigen to the enzymes. After a suitable incubation period, the mixture was added to antiserum, and then paramecia were added to see if the antigen had absorbed antibody from the serum or whether its c ...
PD-1 Pathway, Flyer - Bio
... inhibitor screening assays, and drug discovery. BPS is the only company to offer biotinylated versions of PD-1, ...
... inhibitor screening assays, and drug discovery. BPS is the only company to offer biotinylated versions of PD-1, ...
Identification and Structural Characterization of the ATP/ADP
... GP96 or endoplasmin) localized to the endoplasmic reticulum, where it is involved in the assembly of immunoglobulins (Melnick et al., 1992) and other proteins destined for secretion or surface presentation. GRP94/ GP96 is also involved in the loading of proteosomegenerated antigenic peptides onto na ...
... GP96 or endoplasmin) localized to the endoplasmic reticulum, where it is involved in the assembly of immunoglobulins (Melnick et al., 1992) and other proteins destined for secretion or surface presentation. GRP94/ GP96 is also involved in the loading of proteosomegenerated antigenic peptides onto na ...
The Department of Electrical and Computer Engineering
... Figure 2.4 Illustration of wave function with induced impurity state as a result of modification of (10,0) SWCNT with functional groups (a) COOH-functionalization (b) F-functionalization and (c) a C vacancy on the side wall, where the yellow and blue colors indicate the polarity of the wave function ...
... Figure 2.4 Illustration of wave function with induced impurity state as a result of modification of (10,0) SWCNT with functional groups (a) COOH-functionalization (b) F-functionalization and (c) a C vacancy on the side wall, where the yellow and blue colors indicate the polarity of the wave function ...
Epitope Specificity of Anti-Citrullinated Protein Antibodies
... have been proposed to be divided into two groups, based on their ability to recognize multiple citrullinated peptides. Thus, an “overlapping” antibody group, which appears to recognize several citrullinated peptides, and a “non-overlapping” antibody group, which only recognizes a limited number of c ...
... have been proposed to be divided into two groups, based on their ability to recognize multiple citrullinated peptides. Thus, an “overlapping” antibody group, which appears to recognize several citrullinated peptides, and a “non-overlapping” antibody group, which only recognizes a limited number of c ...
Common Features of Mucosal and Peripheral Antibody Responses
... rhesus IgG1 and IgG3 (kindly provided by K. Reimann, NIH Nonhuman Primate Reagent Resource). Both Ad/Ad and protein vaccines elicited IgG1 and IgG3 responses in serum and mucosal secretions. The Ad vectors elicited comparable titers of IgG3 and IgG1 responses in both sera and mucosal secretions, whe ...
... rhesus IgG1 and IgG3 (kindly provided by K. Reimann, NIH Nonhuman Primate Reagent Resource). Both Ad/Ad and protein vaccines elicited IgG1 and IgG3 responses in serum and mucosal secretions. The Ad vectors elicited comparable titers of IgG3 and IgG1 responses in both sera and mucosal secretions, whe ...
Antibodies to Chlamydia trachomatis heat shock proteins in women
... to hsp antibodies examined. Materials and methods As described elsewhere 163 women were identified with tubal factor infertility (Osser et al., 1989). One age-matched pregnant woman was selected as control for each of the 163 patients. Sera were collected from both groups. Chlamydial antibodies by m ...
... to hsp antibodies examined. Materials and methods As described elsewhere 163 women were identified with tubal factor infertility (Osser et al., 1989). One age-matched pregnant woman was selected as control for each of the 163 patients. Sera were collected from both groups. Chlamydial antibodies by m ...
A Raman spectroscopic study of the interaction between nucleotides
... standard. Corresponding positive or negative contributions are indicated with a double arrow. These features will be discussed. Additional positive protein contributions indicated with a single arrow are observed in the difference spectrum for which the 1447-cm-' band was used as an intensity refere ...
... standard. Corresponding positive or negative contributions are indicated with a double arrow. These features will be discussed. Additional positive protein contributions indicated with a single arrow are observed in the difference spectrum for which the 1447-cm-' band was used as an intensity refere ...
Antibodies to N**-(A^-isopentenyI) adenosine and its nucleotide
... The interaction of antibodies directed toward N B -( A2-isopentenyl )adenosine, i s Ado, or its nucleotide with related bases, nucleosides, nucleotides and purified tRNAs is described. The selectivity of the antibody preparation was tested in inhibition experiments utilizing a sensitive radioimmunoa ...
... The interaction of antibodies directed toward N B -( A2-isopentenyl )adenosine, i s Ado, or its nucleotide with related bases, nucleosides, nucleotides and purified tRNAs is described. The selectivity of the antibody preparation was tested in inhibition experiments utilizing a sensitive radioimmunoa ...
Protein Function and Classification (Cont.) - EMBL-EBI
... What information can be found in the InterPro entry page? ................................. 8 How do I interpret an InterPro protein view? ........................................................ 9 Protein view: Overview page......................................................................... 9 ...
... What information can be found in the InterPro entry page? ................................. 8 How do I interpret an InterPro protein view? ........................................................ 9 Protein view: Overview page......................................................................... 9 ...
Antigen-Specific Antibody Glycosylation Is Regulated via
... dominant subclass in the blood [7]. However, as all vaccinated and infected individuals ultimately produce IgG1 antibodies, it is unclear why some IgG1 responses provide protective immunity while others provide limited immunity at the same titers. While emerging data suggest that the co-selection of ...
... dominant subclass in the blood [7]. However, as all vaccinated and infected individuals ultimately produce IgG1 antibodies, it is unclear why some IgG1 responses provide protective immunity while others provide limited immunity at the same titers. While emerging data suggest that the co-selection of ...
Keyhole Limpet Hemocyanin ( KLH )
... precipitation. A slightly acidic pH (~pH 6.5) and the concentration of MgSO4 (≥10 mM MgSO4 is recommended for most procedures) are essential for facilitating and maintaining solubility. While aggregation may not affect KLH immunogenicity by itself, it affects the real available protein quantity. Hen ...
... precipitation. A slightly acidic pH (~pH 6.5) and the concentration of MgSO4 (≥10 mM MgSO4 is recommended for most procedures) are essential for facilitating and maintaining solubility. While aggregation may not affect KLH immunogenicity by itself, it affects the real available protein quantity. Hen ...
Hijacking Complement Regulatory Proteins for Bacterial Immune
... (Wickham et al., 2011). Structurally, CD59 has no similarity with other complement proteins. CD59 is expressed on a wide range of cells, where it binds to C5b-8 and C5b-9 preventing the formation of the MAC in the lipid bilayer. CD59 cannot bind to free C8 or C9, but specifically interacts with the ...
... (Wickham et al., 2011). Structurally, CD59 has no similarity with other complement proteins. CD59 is expressed on a wide range of cells, where it binds to C5b-8 and C5b-9 preventing the formation of the MAC in the lipid bilayer. CD59 cannot bind to free C8 or C9, but specifically interacts with the ...
Tag-ItTM Mutation Detection Kit for CFTR 70+6
... mutations (could be 2 different mutations) in the gene for CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
... mutations (could be 2 different mutations) in the gene for CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) protein which enhances the secretion of chloride in mucus producing epithelial cells. ...
Combining docking and molecular dynamic simulations in drug design
... high-throughout process.61 Many proteins targeted for drug design do not have an experimentally determined structure and, therefore, docking studies cannot be performed directly. In some cases, computational techniques can be used to predict the 3-D structure of a protein provided the structure of a ...
... high-throughout process.61 Many proteins targeted for drug design do not have an experimentally determined structure and, therefore, docking studies cannot be performed directly. In some cases, computational techniques can be used to predict the 3-D structure of a protein provided the structure of a ...
PDF File
... ABSTRACT: It has been suggested that the fundamental feature that distinguishes enzymes from simple chemical catalysts is the ability of enzymes to use binding interactions for catalysis. Results with the Tetrahymena group I RNA enzyme described herein directly demonstrate the catalytic contribution ...
... ABSTRACT: It has been suggested that the fundamental feature that distinguishes enzymes from simple chemical catalysts is the ability of enzymes to use binding interactions for catalysis. Results with the Tetrahymena group I RNA enzyme described herein directly demonstrate the catalytic contribution ...