Lecture 13 Electrophoresis (Part-I)
... Troubleshooting : The pattern of the protein bands on polyacrylamide gel depend on several factors. As a result several types of defects are observed. Now we will discuss few of these defects and responsible factors. 1. Smiling : Uneven heating of the gel causes differential migration of proteins, w ...
... Troubleshooting : The pattern of the protein bands on polyacrylamide gel depend on several factors. As a result several types of defects are observed. Now we will discuss few of these defects and responsible factors. 1. Smiling : Uneven heating of the gel causes differential migration of proteins, w ...
Bacillus anthracis Protective Antigen Bound by Neutralizing Antibodies *
... from vaccinated humans and rabbits (6, 12). The cellular receptor binding region is localized to the small loop of domain 4, and this region has been described to be recognized by two neutralizing mAbs (7, 9). With the exception of a neutralizing mAb that bound to PA20 (13), no B-cell epitopes have ...
... from vaccinated humans and rabbits (6, 12). The cellular receptor binding region is localized to the small loop of domain 4, and this region has been described to be recognized by two neutralizing mAbs (7, 9). With the exception of a neutralizing mAb that bound to PA20 (13), no B-cell epitopes have ...
ELUCIDATING THE ROLE OF SERUM IgA
... SLE is a chronic autoimmune disease in which auto-antibodies produced are directed against self-antigens (nuclear components), causing widespread immune complex deposition in various tissues in the body. In addition, SLE patients exhibit poor clearance of such immune complexes due to deficient seru ...
... SLE is a chronic autoimmune disease in which auto-antibodies produced are directed against self-antigens (nuclear components), causing widespread immune complex deposition in various tissues in the body. In addition, SLE patients exhibit poor clearance of such immune complexes due to deficient seru ...
Polysaccharide-protein conjugate vaccines
... induction folloWing immunization by these antigens. [0004] T-independent polysaccharide antigens can be con verted to T-dependent antigens by covalent attachment of the polysaccharides to protein molecules. B cells that bind the polysaccharide component of the conjugate vaccine can be activated by h ...
... induction folloWing immunization by these antigens. [0004] T-independent polysaccharide antigens can be con verted to T-dependent antigens by covalent attachment of the polysaccharides to protein molecules. B cells that bind the polysaccharide component of the conjugate vaccine can be activated by h ...
Kirvan, et al (2003) Mimicry and Auto-antibody
... 31.1.1 and 37.2.1) that reacted strongly with glutaraldehyde-fixed, whole-cell type 5 S. pyogenes18. Monoclonal antibody reactivity was subsequently localized to GlcNAc, the immunodominant epitope of the GAS-specific carbohydrate that is a major constituent of the GAS cell wall (Fig. 1). Chorea anti ...
... 31.1.1 and 37.2.1) that reacted strongly with glutaraldehyde-fixed, whole-cell type 5 S. pyogenes18. Monoclonal antibody reactivity was subsequently localized to GlcNAc, the immunodominant epitope of the GAS-specific carbohydrate that is a major constituent of the GAS cell wall (Fig. 1). Chorea anti ...
Immunogenicity - Biologics and Biosimilars
... To have sufficient sensitivity and specificity for non GLP Be validated for GLP studies Neutralizing Abs are typically not done in non-human primates but is considered in a case by case basis and depends upon a variety of factors that include: Type of drug and MOA ADA incidence Toxicity PK d ...
... To have sufficient sensitivity and specificity for non GLP Be validated for GLP studies Neutralizing Abs are typically not done in non-human primates but is considered in a case by case basis and depends upon a variety of factors that include: Type of drug and MOA ADA incidence Toxicity PK d ...
HIS-Select Nickel Affinity Gel (P6611) - Technical - Sigma
... 2. Carefully remove the supernatant and discard. 3. Add 200 µl of Equilibration Buffer without imidazole and mix well. 4. Centrifuge for 30 seconds at 5,000 × g. Remove and discard the supernatant. 5. Add 100 µl of clarified recombinant protein solution and gently mix for 1 minute. Centrifuge the mi ...
... 2. Carefully remove the supernatant and discard. 3. Add 200 µl of Equilibration Buffer without imidazole and mix well. 4. Centrifuge for 30 seconds at 5,000 × g. Remove and discard the supernatant. 5. Add 100 µl of clarified recombinant protein solution and gently mix for 1 minute. Centrifuge the mi ...
Introduction to Diagnostic and Therapeutic Monoclonal Antibodies
... even more potential, as common obstacles to their use are resolved. The route that this biotechnology routinely follows is to first be introduced in specialized situations that do not involve radiolabeling. Then, as the safety of each antibody product is established, uses targeting the specific site ...
... even more potential, as common obstacles to their use are resolved. The route that this biotechnology routinely follows is to first be introduced in specialized situations that do not involve radiolabeling. Then, as the safety of each antibody product is established, uses targeting the specific site ...
Detection of antibodies to common antigens of pathogenic and
... and Poolman (1987) was used. N . meningitidis B, 15P1.16 and N . lactamica were cultured overnight in Tryptone Soya Broth (Oxoid, CM129) at 36"C, on a gyratory shaker, before being heat-inactivated (56°C for 1 h) and coated on to microtitration plates (M129A Dynatech Laboratories Ltd, Billingshurst) ...
... and Poolman (1987) was used. N . meningitidis B, 15P1.16 and N . lactamica were cultured overnight in Tryptone Soya Broth (Oxoid, CM129) at 36"C, on a gyratory shaker, before being heat-inactivated (56°C for 1 h) and coated on to microtitration plates (M129A Dynatech Laboratories Ltd, Billingshurst) ...
Analytical challenges of antibody-drug conjugates
... Main processes with bioanalytical relevance: 1. Antibody-related (mainly intracellular catabolism) • Like antibodies, ADCs are specifically taken up into cells via target antigens plus unspecifically via Fc and FcRn receptors (“on-target/off-target”). • Besides tumor targets cells, also non-tumor ce ...
... Main processes with bioanalytical relevance: 1. Antibody-related (mainly intracellular catabolism) • Like antibodies, ADCs are specifically taken up into cells via target antigens plus unspecifically via Fc and FcRn receptors (“on-target/off-target”). • Besides tumor targets cells, also non-tumor ce ...
Gel Electrophoresis
... Agarose is a polysaccharide obtained from red seaweed. The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relativ ...
... Agarose is a polysaccharide obtained from red seaweed. The pore size depends on the concentration of agarose (weight of agarose per volume). Agarose is dissolved in boiling water and forms a gel during cooling. During this process, double helices are built, which are joined laterally to form relativ ...
Analysis of the Original Antigenic Sin Antibody Response to the
... membrane protein (MOMP) of the priming and challenge C. trachomatis serovars and that the recall response to the original serovar is correlated with the extent of sequence sharing between the two serovars [16]. They also demonstrated that OAS antibody responses are associated with the development of ...
... membrane protein (MOMP) of the priming and challenge C. trachomatis serovars and that the recall response to the original serovar is correlated with the extent of sequence sharing between the two serovars [16]. They also demonstrated that OAS antibody responses are associated with the development of ...
The coupling modification of gABA Flyer (, 0.15 kB)
... The OVA-gABA was used as the antigen to immunize rabbit to prepare antiserum, after purification, specific anti-gABA polyclonal antibodies were obtained (PAA900Ge01). And then, the antibody was labeled by biotin to prepare biotin-linked antibody to gABA (PAA900Ge71). Biotin labeling method: ...
... The OVA-gABA was used as the antigen to immunize rabbit to prepare antiserum, after purification, specific anti-gABA polyclonal antibodies were obtained (PAA900Ge01). And then, the antibody was labeled by biotin to prepare biotin-linked antibody to gABA (PAA900Ge71). Biotin labeling method: ...
Plaque reduction test: an alternative method to assess specific
... used to evaluate the binding specificity of selected phage to purified IgG. In Western blot analysis, the phage proteins were separated by SDS-PAGE (10% polyacrylamide), transferred onto nitrocellulose membranes, and incubated with purified IgG. The color development was visualized with the NBT/BCIP ...
... used to evaluate the binding specificity of selected phage to purified IgG. In Western blot analysis, the phage proteins were separated by SDS-PAGE (10% polyacrylamide), transferred onto nitrocellulose membranes, and incubated with purified IgG. The color development was visualized with the NBT/BCIP ...
study of the anti – pregnancy
... consequence of interaction between B lymphocyte and specific antigens (Abbas et al., 2000; Tizard, 2004). The antibody has an ability to bind a specific antigen. Antibody exists in several body liquids but the highest concentration is in blood serum, from where is the easiest to collect (Tizard, 200 ...
... consequence of interaction between B lymphocyte and specific antigens (Abbas et al., 2000; Tizard, 2004). The antibody has an ability to bind a specific antigen. Antibody exists in several body liquids but the highest concentration is in blood serum, from where is the easiest to collect (Tizard, 200 ...
The role of carbohydrate in the antigenic and immunogenic structure
... and glV is carbohydrate-dependent. Loss of carbohydrate from gI also resulted in a significantly decreased ability to induce a serum neutralizing antibody response to BHV-1, due to modifications in three distinct carbohydrate-containing continuous epitopes. Similarly, in vitro lysis of BHV-l-infecte ...
... and glV is carbohydrate-dependent. Loss of carbohydrate from gI also resulted in a significantly decreased ability to induce a serum neutralizing antibody response to BHV-1, due to modifications in three distinct carbohydrate-containing continuous epitopes. Similarly, in vitro lysis of BHV-l-infecte ...
Interferences in Immunoassays
... are not acceptable for such assays. Because hemolysis may also interfere with some signal generation steps of different types of immunoassays, grossly hemolysed specimens should not be used. Lipemia can interfere in some immunoassays especially those using nephelometry and turbidimetry. Lipemia of s ...
... are not acceptable for such assays. Because hemolysis may also interfere with some signal generation steps of different types of immunoassays, grossly hemolysed specimens should not be used. Lipemia can interfere in some immunoassays especially those using nephelometry and turbidimetry. Lipemia of s ...
antibody structure and function
... respond to artificial antigens that do not exist in nature (Fast Focus 1). Because the amino acid sequence differs in the arms of various antibody molecules, each different antibody can bind specifically to 1We use the term antigen, even though immunogen may be more accurate at times, as explained i ...
... respond to artificial antigens that do not exist in nature (Fast Focus 1). Because the amino acid sequence differs in the arms of various antibody molecules, each different antibody can bind specifically to 1We use the term antigen, even though immunogen may be more accurate at times, as explained i ...
Introduction to Immunoassays
... • The animals produce the antiserum - just as a human would - as a defense mechanism when exposed to an antigen. • Antiserum contains a mixture of antibodies, each of which may bind to different antigen binding sites, or epitopes. ...
... • The animals produce the antiserum - just as a human would - as a defense mechanism when exposed to an antigen. • Antiserum contains a mixture of antibodies, each of which may bind to different antigen binding sites, or epitopes. ...
Cell-free eukaryotic systems for the production
... is nonphosphorylated SMAD2 peptide), SMAD2-P (where SMAD2-P is phosphorylated SMAD2 peptide), and CXC chemokine receptor type 5 (CXCR5) (SH527-IIA4, SH527IIC10, and SH855-C11, Table 1) were selected by phage display using the human naive antibody gene library HAL7/8 [30] as described before [31]. Li ...
... is nonphosphorylated SMAD2 peptide), SMAD2-P (where SMAD2-P is phosphorylated SMAD2 peptide), and CXC chemokine receptor type 5 (CXCR5) (SH527-IIA4, SH527IIC10, and SH855-C11, Table 1) were selected by phage display using the human naive antibody gene library HAL7/8 [30] as described before [31]. Li ...
Modern affinity reagents: Recombinant antibodies and aptamers
... There are four main types of libraries that differ by the source of the sequences used to build the library: immune, naïve, synthetic, and semisynthetic (Geyer et al., 2012; Harel Inbar and Benhar, 2012). Immune antibody libraries are generated using active B cells from an immunized human donor or a ...
... There are four main types of libraries that differ by the source of the sequences used to build the library: immune, naïve, synthetic, and semisynthetic (Geyer et al., 2012; Harel Inbar and Benhar, 2012). Immune antibody libraries are generated using active B cells from an immunized human donor or a ...
F1-ATPase`
... Isolation and Purification of Corn F,-ATPase. The chloroform release method introduced by Beechey et al. (2) was used to release Fr-ATPase from SMP. However, in order to obtain reproducible results from this procedure, the special conditions of slow passage through chloroform described in "Materials ...
... Isolation and Purification of Corn F,-ATPase. The chloroform release method introduced by Beechey et al. (2) was used to release Fr-ATPase from SMP. However, in order to obtain reproducible results from this procedure, the special conditions of slow passage through chloroform described in "Materials ...
The use of antibodies in the treatment of infectious diseases
... There is a long history of the use of antibodies in the treatment and prophylaxis of infectious diseases, because these molecules play a critical role in directing the effector mechanisms of the immune system against the pathogens they recognise. However, the widespread application of this therapy h ...
... There is a long history of the use of antibodies in the treatment and prophylaxis of infectious diseases, because these molecules play a critical role in directing the effector mechanisms of the immune system against the pathogens they recognise. However, the widespread application of this therapy h ...
A Chemical Approach To Illustrate the Principal of Signal
... for signal transduction. Generally, the sequential process is initiated by the binding of an extracellular signal to a receptor culminating in one or more specific cellular responses In this way, a signal, for example, can be transferred from the outside of the cell en route to the nucleus—a process ...
... for signal transduction. Generally, the sequential process is initiated by the binding of an extracellular signal to a receptor culminating in one or more specific cellular responses In this way, a signal, for example, can be transferred from the outside of the cell en route to the nucleus—a process ...
Complement evasion strategies of pathogens
... C3 convertase (Blom et al., 2004). In addition C4BP contributes as a FI cofactor to the cleavage of C3b and may down-regulate the alternative pathway (Blom et al., 2003). C4BP is a large plasma protein consisting of seven identical ␣-chains and a unique -chain, which are covalently linked together ...
... C3 convertase (Blom et al., 2004). In addition C4BP contributes as a FI cofactor to the cleavage of C3b and may down-regulate the alternative pathway (Blom et al., 2003). C4BP is a large plasma protein consisting of seven identical ␣-chains and a unique -chain, which are covalently linked together ...