Protein Folding, Shape, and Function Activity Instructions
... A chart of the amino acid sidechains and their properties is available for reference. 5. In addition to using the principles above to fold your protein, also form a pocket or groove (representing an active site) around a small lego (representing the substrate). This active site and substrate should ...
... A chart of the amino acid sidechains and their properties is available for reference. 5. In addition to using the principles above to fold your protein, also form a pocket or groove (representing an active site) around a small lego (representing the substrate). This active site and substrate should ...
Chapter 6 - Dr. Jennifer Capers
... E. decrease beta-tubulin III (found in developing neurons, if found in other tissue it can be used as cancer marker) F. decrease thymidilate kinase (needed by tumor cell to repair DNA) ...
... E. decrease beta-tubulin III (found in developing neurons, if found in other tissue it can be used as cancer marker) F. decrease thymidilate kinase (needed by tumor cell to repair DNA) ...
Pipe Cleaner Protein Folding Activity My
... functions. In this activity, students will identify the four levels of protein structure. Students will understand how changes in the amino acids can affect the shape and overall function of a protein by developing a simple model using beads and pipe cleaner. ...
... functions. In this activity, students will identify the four levels of protein structure. Students will understand how changes in the amino acids can affect the shape and overall function of a protein by developing a simple model using beads and pipe cleaner. ...
Surface-activated Dynabeads
... of the ligand should also be taken into consideration. Hydrophobic beads facilitate interactions with hydrophobic parts of a protein, while hydrophilic beads are better suited when an interaction with hydrophilic parts of the protein is desired. →→ Manual or automated protocols: The 1 μm Dynabeads® ...
... of the ligand should also be taken into consideration. Hydrophobic beads facilitate interactions with hydrophobic parts of a protein, while hydrophilic beads are better suited when an interaction with hydrophilic parts of the protein is desired. →→ Manual or automated protocols: The 1 μm Dynabeads® ...
Tag-ChIP-IT® Enables ChIP Without Protein
... No ChIP-validated antibody for your protein of interest? No problem! Transcription factor ChIP is often challenging due to a lack of available antibodies that are capable of recognizing target-bound protein of interest post-fixation, or the inability of available antibodies to distinguish between pr ...
... No ChIP-validated antibody for your protein of interest? No problem! Transcription factor ChIP is often challenging due to a lack of available antibodies that are capable of recognizing target-bound protein of interest post-fixation, or the inability of available antibodies to distinguish between pr ...
mcnair 2003 poster template
... The beads, along with the attached antibodies and antigens, are pulled to the side of the tube. The rest of the solution is pipetted off. The isolated protein can then be eluted for use in experiments. For this experiment, the goal is to isolate the entire complex of proteins associated with dynein ...
... The beads, along with the attached antibodies and antigens, are pulled to the side of the tube. The rest of the solution is pipetted off. The isolated protein can then be eluted for use in experiments. For this experiment, the goal is to isolate the entire complex of proteins associated with dynein ...
ChIP Lysis Buffer High Salt: sc-45001 Material Safety Data Sheet
... Sonicate on ice at power output setting = 5–6, continuous mode, 4 times at 30 second intervals. ...
... Sonicate on ice at power output setting = 5–6, continuous mode, 4 times at 30 second intervals. ...
Puredown Protein A/G-Agarose Conjugate
... Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. I ...
... Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. I ...
Expediting Purification of Cellular Proteins
... molecules or cellular events to global functional analysis, feeding these results into new approaches for the prevention, diagnosis, and treatment of cancer. Methods that allow researchers to look across a broader angle at cellular processes such as mRNA expression levels or protein interaction patt ...
... molecules or cellular events to global functional analysis, feeding these results into new approaches for the prevention, diagnosis, and treatment of cancer. Methods that allow researchers to look across a broader angle at cellular processes such as mRNA expression levels or protein interaction patt ...
Discovering Macromolecular Interactions
... Immunoprecipitation is one of the most widely used methods for isolation of proteins and other biomolecules from cell or tissue lysates for the purpose of subsequent detection by western blotting and other assay techniques. Because it developed as an adaptation of column affinity chromatography, the ...
... Immunoprecipitation is one of the most widely used methods for isolation of proteins and other biomolecules from cell or tissue lysates for the purpose of subsequent detection by western blotting and other assay techniques. Because it developed as an adaptation of column affinity chromatography, the ...
Please visit Cell Signaling Technology at Both 18.
... conjugated with capture antibodies and incubated with lysates from cancer cell lines treated with agents that affect the phosphorylation state of target proteins. Captured phospho proteins were labeled with biotinconjugated phospho-sensitive detection antibodies and streptavidin-PE. The bead complex ...
... conjugated with capture antibodies and incubated with lysates from cancer cell lines treated with agents that affect the phosphorylation state of target proteins. Captured phospho proteins were labeled with biotinconjugated phospho-sensitive detection antibodies and streptavidin-PE. The bead complex ...
北京聚合美生物科技有限公司 Mei5 Biotechnology, Co., Ltd M5
... 9. Dilute the cell lysate to approximately 1 μg/μl total cell protein with PBS to reduce the concentration of the detergents in the buffer. A more concentrated cell lysate (i.e., 10 μg/μl) may be necessary to immunoprecipitate a protein, which is found in low levels in a cell model. 10. Add the reco ...
... 9. Dilute the cell lysate to approximately 1 μg/μl total cell protein with PBS to reduce the concentration of the detergents in the buffer. A more concentrated cell lysate (i.e., 10 μg/μl) may be necessary to immunoprecipitate a protein, which is found in low levels in a cell model. 10. Add the reco ...
Typical IP Protocol
... Antibodies Analytical Techniques Utilizing Antibodies: • flow cytometry • gel electrophoresis • immunoprecipitation (IP) • immunoblotting • microscopy • immunofluorescence (IFA) • electron microscopy • ELISA • antibodies bind proteins with high specificity and affinity • affinity chromatography • an ...
... Antibodies Analytical Techniques Utilizing Antibodies: • flow cytometry • gel electrophoresis • immunoprecipitation (IP) • immunoblotting • microscopy • immunofluorescence (IFA) • electron microscopy • ELISA • antibodies bind proteins with high specificity and affinity • affinity chromatography • an ...
Immobilization of Membrane Proteins on Beads
... biomolecules. Agarose, paramagnetic, and polystyrene beads enable attached proteins to be used as highly-pure targets within a number of assay formats, including chromatography, centrifugation, or magnetic separation. Due to their conformational requirements for an intact membrane, integral membrane ...
... biomolecules. Agarose, paramagnetic, and polystyrene beads enable attached proteins to be used as highly-pure targets within a number of assay formats, including chromatography, centrifugation, or magnetic separation. Due to their conformational requirements for an intact membrane, integral membrane ...
His-tag pull-down assay Possible interaction between PprI protein
... Possible interaction between PprI protein and N-terminal part of DdrO(N-DdrO, a.a. 1-108)were tested using His-tag pull-down assay. 20 µg of purified N-DdrO protein with N-terminal His-tag was incubated with Ni-NTA agarose beads in 1 ml of pull-down buffer (167mM NaCl, 20 mM Tris-HCl pH 8.0, 5% glyc ...
... Possible interaction between PprI protein and N-terminal part of DdrO(N-DdrO, a.a. 1-108)were tested using His-tag pull-down assay. 20 µg of purified N-DdrO protein with N-terminal His-tag was incubated with Ni-NTA agarose beads in 1 ml of pull-down buffer (167mM NaCl, 20 mM Tris-HCl pH 8.0, 5% glyc ...
Gold Biotechnology Enzyme and Antibody Immobilization
... • Glyoxal Agarose Beads: Supports with an aldehyde group that covalently reacts with the lysine groups in the biomolecules. • Aminoethyl Agarose Beads: Supports with an amino group that covalently reacts with acidic amino acids like aspartic acid or glutamic acid. Both types of resins give the biomo ...
... • Glyoxal Agarose Beads: Supports with an aldehyde group that covalently reacts with the lysine groups in the biomolecules. • Aminoethyl Agarose Beads: Supports with an amino group that covalently reacts with acidic amino acids like aspartic acid or glutamic acid. Both types of resins give the biomo ...
Conjugation antibody and Magnetic bead
... Transfer supernatant to a fresh tube. Measure the protein concentration. Aliqout min1000 µg protein (500 µg protein is also enough for some proteins, you should optimize for your protein) containing cell lysate to each tube. Take out 50ul from each sample for loading to gel as total lysate co ...
... Transfer supernatant to a fresh tube. Measure the protein concentration. Aliqout min1000 µg protein (500 µg protein is also enough for some proteins, you should optimize for your protein) containing cell lysate to each tube. Take out 50ul from each sample for loading to gel as total lysate co ...
Supplemental Materials and Methods
... tetracycline, IPTG, and X-gal per the manufacturer’s protocols. White colonies were selected from these plates, and bacmid DNA was generated by alkaline lysis plasmid preparation and verified by PCR amplification across the bacmid junctions. Proteins were expressed in either insect SF5 or HEK293 mam ...
... tetracycline, IPTG, and X-gal per the manufacturer’s protocols. White colonies were selected from these plates, and bacmid DNA was generated by alkaline lysis plasmid preparation and verified by PCR amplification across the bacmid junctions. Proteins were expressed in either insect SF5 or HEK293 mam ...