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Dynabeads® MyOne™ Carboxylic Acid
Dynabeads® MyOne™ Carboxylic Acid

... cross-linkers may be used to introduce other functional groups like thiol, amine, maleimide, etc., onto the surface of the beads. This may be useful when coupling ligands which do not have available primary amine groups. Activation and coupling may be performed in organic solvents such as DMF or ace ...
About Proteins
About Proteins

... body  The order of the AAs determines the function  If even one AA is out of order by mistake, the protein will not function (work)  This is because proteins fold in a specific way ...
BMT 242 Immunology
BMT 242 Immunology

... Enzyme digestion fragments ...
Affinity Chromatography
Affinity Chromatography

... Trp130 C-terminal domain ...
TRPA1 antibody - middle region (ARP35205_P050)
TRPA1 antibody - middle region (ARP35205_P050)

... This is a rabbit polyclonal antibody against TRPA1. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies ...
Fusion, Affinity and Epitope Tags Lecture Notes Handout
Fusion, Affinity and Epitope Tags Lecture Notes Handout

... Secreted into periplamsic space of E. coli where disulfide bonds can more favorably form ü This decreases potential yield but may help folding for some high disulfide containing proteins th 1/5 of MBP fusion proteins do not bind to affinity resin MBP can help expression by increasing solubility mor ...
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... Comparison of Drosophila embryos stained with anti-ETO Ab-1 and anti-Nvy (Fig. 2 in main text). Overnight collections of nvyPDFKG1/CyO, Kr>GFP or Oregon-R embryos were fixed and prepared for confocal microscopy as previously described (1). Rabbit anti-ETO was used at the same concentration as previo ...
TDS - BD Biosciences
TDS - BD Biosciences

... β-Arrestins were discovered due to their ability to modulate interactions between the phosphorylated β2-Adrenergic receptors and G proteins. This modulation results in diminished β2-Adrenergic receptor function, also known as desensitization. Because arrestins are found at the synaptic terminals, th ...
Western blot, fehérjék elektroforézise, fehérjechip
Western blot, fehérjék elektroforézise, fehérjechip

... Lane 1 - molecular weight marker Lane 2 - positive patient sample Lane 3 - positive patient sample Lane 4 - monoclonal antibodies for 39 and 41kD bands Lane 5 - monoclonal antibodies for ...
Product PDF for Catalog Number: 103-M452
Product PDF for Catalog Number: 103-M452

... TSG14 forms both pentameric and higher ordered oligomers. Similar to CRP and SAP, TSG14 binds to the complement cascade component C1q. However, TSG14 does not bind to phosphoethanolamine, phosphocholine, or high pyruvate agarose, which are known ligands for CRP and SAP. While CRP and SAP are primari ...
Anti-UBR1 Antibody
Anti-UBR1 Antibody

... Dilutions are for reference only. Applications not listed above are not necessarily precluded from working with this antibody. Investigators intending to use an application that has not been verified can request a complimentary sample. ...
Anti-Ribosomal Protein L26 (N-terminal) (R0655)
Anti-Ribosomal Protein L26 (N-terminal) (R0655)

... surface of the ribosome while the rRNA components make up the central core. rRNAs play a central part in the ribosome catalytic activities. The proteins’ main function is to hold the ribosomal RNA in place so that it could carry out its catalytic activity.1 However, being at the surface of the ribos ...
DN: Protein
DN: Protein

... sequence of the 20 different amino acids as illustrated on the left. In the feed lab, protein is distinguishable from carbohydrate and lipid due to its content of nitrogen (N) feed proteins typically contain about 16% N. This property makes it possible to estimate the protein content of a feedstuff ...
Protein Purification and Characterization Techniques
Protein Purification and Characterization Techniques

... • Extent of crosslinking & pore size (exclusion limit) • Convenient & MW estimate • Each gel has range of sizes that separate linearly with the log of the molecular weight ...
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... Protein depletion by genetic means, in a very general sense including the use of RNA interference [1, 2] or CRISPR/Cas9-based methods, represents a central paradigm of modern biology to study protein functions in vivo. However, acting upstream the proteic level is a limiting factor if the turnover o ...
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E. coli

... 1. Prepare E. coli cell extract (competitor, to remove background). 2. Mix 35S-radiolabelled test protein and E. coli cell extract. Incubate 15 min on ice. 3. Add GST or GST-protein bound to agarose beads to cell extract in an eppendorf tube, Mix. 4. Add 3. to 2. Incubate 1-2 hr at 40C. 5. Centrifug ...
TMPRSS4 antibody - middle region (ARP49463_P050)
TMPRSS4 antibody - middle region (ARP49463_P050)

... Biology. At Aviva Systems Biology we manufacture rabbit polyclonal antibodies on a large scale (200-1000 products/month) of high throughput manner. Our antibodies are peptide based and protein family oriented. We usually provide antibodies covering each member of a whole protein family of your inter ...
OAS1 antibody - middle region (ARP51359_P050) Data Sheet
OAS1 antibody - middle region (ARP51359_P050) Data Sheet

... replication.This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'oligoade ...
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... Cryogenic protein storage and assessment of protein purity Flash freezing of protein for long term storage. Mass spectrometry and SDS-PAGE for determination of purity and molecular weight. Preparation of buffers for experiments in following weeks. ...
Bio4751signaltransductionTechniques
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DAAM1 antibody - middle region (ARP55131_P050)
DAAM1 antibody - middle region (ARP55131_P050)

... implicated in cell polarity. Wnt/Fz signaling activates the small GTPase Rho, a key regulator of cytoskeleton architecture, to control cell polarity and movement during development. Activation requires Dvl-Rho complex formation, an assembly mediated by this gene product, which is thought to function ...
magnetic GFP-Trap -M for Immunoprecipitation of GFP
magnetic GFP-Trap -M for Immunoprecipitation of GFP

... Green fluorescent proteins (GFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these GFP fusion proteins and their interacting factors can be isolated fast and efficiently (one st ...
MYOZAP Antibody
MYOZAP Antibody

... Supplied in PBS containing 0.02% sodium azide. ...
Flow Cytometry Protein A and Protein G Antibody Binding Beads
Flow Cytometry Protein A and Protein G Antibody Binding Beads

... 3. Schwartz, A., G.E. Marti, R. Poon, J.W. Gratama, E. Fernandez Repollet. 1998. Standardizing flow cytometry: a classification system of ...
PAK1 PBD Agarose Beads
PAK1 PBD Agarose Beads

... Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways. Rac, a 21 kDa protein, belongs to the family of Rho GTPases regulating a variety of biological response pathways that include cell motility, cell division, gene tra ...
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Immunoprecipitation

Immunoprecipitation (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins. Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure.
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