Cube Biotech
... The rho1D4 epitope and antibody pair was characterized in the 1980ꞌs and used to purify bovine rhodopsin expressed in monkey kidney cells by coupling the antibody to Sepharose® beads.(1,2) Since then, the rho1D4 system (tag, antibody-coupled affinity matrix, eluent peptide) has been used to study a ...
... The rho1D4 epitope and antibody pair was characterized in the 1980ꞌs and used to purify bovine rhodopsin expressed in monkey kidney cells by coupling the antibody to Sepharose® beads.(1,2) Since then, the rho1D4 system (tag, antibody-coupled affinity matrix, eluent peptide) has been used to study a ...
The Human Cell Poster Introduction
... that really do the heavy lifting. While there are around 20,000 genes encoded in our DNA, the total number of proteins is estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, altern ...
... that really do the heavy lifting. While there are around 20,000 genes encoded in our DNA, the total number of proteins is estimated to be many times more—possibly as many as a million*. This is because a single gene might produce multiple variants of a particular protein through, for example, altern ...
AXL_Instruction
... Background Information The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. ...
... Background Information The protein encoded by this gene is a member of the Tyro3-Axl-Mer (TAM) receptor tyrosine kinase subfamily. The encoded protein possesses an extracellular domain which is composed of two immunoglobulin-like motifs at the N-terminal, followed by two fibronectin type-III motifs. ...
MPS1 Antibody / Metallopan-stimulin 1 (R32561)
... gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm ...
... gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S27E family of ribosomal proteins. It contains a C4-type zinc finger domain that can bind to zinc. The encoded protein has been shown to be able to bind to nucleic acid. It is located in the cytoplasm ...
CD10, Clone SJ5-1B4 mAb
... Instructions for use This antibody has been titred for application in flow cytometry. 5 µl is the maximum amount of reagent required for 1 x 106 cells. Lesser amounts of antibody may be sufficient and it is recommended that the customer determine the optimum amount of antibody for each application. ...
... Instructions for use This antibody has been titred for application in flow cytometry. 5 µl is the maximum amount of reagent required for 1 x 106 cells. Lesser amounts of antibody may be sufficient and it is recommended that the customer determine the optimum amount of antibody for each application. ...
PureCube Rho1D4 Agarose
... The rho1D4 epitope and antibody pair was characterized in the 1980ꞌs and used to purify bovine rhodopsin expressed in monkey kidney cells by coupling the antibody to Sepharose® beads.(1,2) Since then, the rho1D4 system (tag, antibody-coupled affinity matrix, eluent peptide) has been used to study a ...
... The rho1D4 epitope and antibody pair was characterized in the 1980ꞌs and used to purify bovine rhodopsin expressed in monkey kidney cells by coupling the antibody to Sepharose® beads.(1,2) Since then, the rho1D4 system (tag, antibody-coupled affinity matrix, eluent peptide) has been used to study a ...
New Platform Technology for the Development of Therapeutic
... for the development of therapeutic proteins and antibodies, which have been established in our group for the last several years. Those are 1) Novel protein scaffold development as an alternative to antibody and 2) Interfering transbody, which can penetrate into living cells and selectively degrade t ...
... for the development of therapeutic proteins and antibodies, which have been established in our group for the last several years. Those are 1) Novel protein scaffold development as an alternative to antibody and 2) Interfering transbody, which can penetrate into living cells and selectively degrade t ...
Hydrophobic Interaction Chromatography (HIC
... Hydrophobic* Interaction Chromatography (HIC) protocol for the protein purification station *Hydrophobic literally means “water fearing.” It refers to substances that do not mix well with water (i.e., Oil is hydrophobic). Note how it stays together in small beads and does not mix with water. Purpose ...
... Hydrophobic* Interaction Chromatography (HIC) protocol for the protein purification station *Hydrophobic literally means “water fearing.” It refers to substances that do not mix well with water (i.e., Oil is hydrophobic). Note how it stays together in small beads and does not mix with water. Purpose ...
Chapter19
... lenses, mirrors, and acousto/electro-optical devices that can be controlled via computer. ...
... lenses, mirrors, and acousto/electro-optical devices that can be controlled via computer. ...
Bacteria on target
... When ferric citrate binds, FecA activates periplasmic FecR, which then activates the sigma factor FecI, resulting in gene expression ...
... When ferric citrate binds, FecA activates periplasmic FecR, which then activates the sigma factor FecI, resulting in gene expression ...
Slide 1
... When ferric citrate binds, FecA activates periplasmic FecR, which then activates the sigma factor FecI, resulting in gene expression ...
... When ferric citrate binds, FecA activates periplasmic FecR, which then activates the sigma factor FecI, resulting in gene expression ...
Human Proteome advertising miniposter (PDF)
... part of the foreign target, the antigen. The unique properties of antibodies are used in a wide range of therapeutic and research applications. This poster describes some of the most common techniques. ...
... part of the foreign target, the antigen. The unique properties of antibodies are used in a wide range of therapeutic and research applications. This poster describes some of the most common techniques. ...
Analytical Sciences, Poster AS-101 Kinetics and identification of non
... is involved in processes such as proliferation, differentiation and programmed cell death. Stress stimulates the MAP kinase signaling pathway where MAPK8 is activated and in turn phosphorylates a number of transcription factors. The two other proteins are commonly applied as a protein expression tag ...
... is involved in processes such as proliferation, differentiation and programmed cell death. Stress stimulates the MAP kinase signaling pathway where MAPK8 is activated and in turn phosphorylates a number of transcription factors. The two other proteins are commonly applied as a protein expression tag ...
Immunoprecipitation protocol
... 3. Meanwhile prepare the Sepharose beads. If using a monoclonal antibody choose protein G-coupled Sepharose beads. If using a polyclonal antibody, protein Acoupled Sepharose beads are usually suitable (please refer to ‘Choosing the protein beads’ table below). If the beads come as a powder, incubate ...
... 3. Meanwhile prepare the Sepharose beads. If using a monoclonal antibody choose protein G-coupled Sepharose beads. If using a polyclonal antibody, protein Acoupled Sepharose beads are usually suitable (please refer to ‘Choosing the protein beads’ table below). If the beads come as a powder, incubate ...
Lecture_3. ppt - Department of Molecular & Cell Biology
... • Millions of B cell clones w/ specific cell-surface receptors • Activation of B cell clones by specific target antigen • Activated B cells secrete specific antibodies ...
... • Millions of B cell clones w/ specific cell-surface receptors • Activation of B cell clones by specific target antigen • Activated B cells secrete specific antibodies ...
1.16891 / 1.16886 Fractogel® EMD COO
... Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and chemical stability. Since the functional ion excha ...
... Ion Exchange chromatography using weak cation exchangers Fractogel® ion exchangers are cross-linked polymethacrylate resins with pore sizes of about 800 Å modified according to the tentacle technology. The Fractogel® beads have a high mechanical and chemical stability. Since the functional ion excha ...
Western Blotting
... Using the characteristic of high affinity and specificity of antibody, we can detect or quantitative the antigen. Keep in mind that the antibody is protein, can also be recognized as an antigen. The major principle to determine the antigen-antibody interaction is to separate the bound form of antige ...
... Using the characteristic of high affinity and specificity of antibody, we can detect or quantitative the antigen. Keep in mind that the antibody is protein, can also be recognized as an antigen. The major principle to determine the antigen-antibody interaction is to separate the bound form of antige ...
ProteinChipâ technology is one of the most exciting advancements
... ProteinChip technology is one of the most exciting advancements in protein analysis in the last 5 years. The Protein Biology SystemTM (PBS) combines the power of mass analysis with chromatography surfaces on an integrated platform. The PBS can easily be used by biologists, biochemists, and clinicia ...
... ProteinChip technology is one of the most exciting advancements in protein analysis in the last 5 years. The Protein Biology SystemTM (PBS) combines the power of mass analysis with chromatography surfaces on an integrated platform. The PBS can easily be used by biologists, biochemists, and clinicia ...
Nickel Affinity Chromatography Protocol/Guide
... Theory and Introduction: Ni-Affinity Chromatography uses the ability of His to bind nickel. Six histadine amino acids at the end of a protein (either N or C terminus) is known as a 6X His tag. Nickel is bound to an agarose bead by chelation using nitroloacetic acid (NTA) beads. Several companies pro ...
... Theory and Introduction: Ni-Affinity Chromatography uses the ability of His to bind nickel. Six histadine amino acids at the end of a protein (either N or C terminus) is known as a 6X His tag. Nickel is bound to an agarose bead by chelation using nitroloacetic acid (NTA) beads. Several companies pro ...
GRAMD2 antibody - middle region (ARP44634_P050)
... This is a rabbit polyclonal antibody against GRAMD2. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies ...
... This is a rabbit polyclonal antibody against GRAMD2. It was validated on Western Blot using a cell lysate as a positive control. Aviva Systems Biology strives to provide antibodies covering each member of a whole protein family of your interest. We also use our best efforts to provide you antibodies ...
Goat Anti-Rabbit IgG Magnetic Beads | NEB
... 1. The amount of antibody required for optimal coating of the beads will vary with the PAb affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis. ...
... 1. The amount of antibody required for optimal coating of the beads will vary with the PAb affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis. ...
Purification of Antibodies
... 3) Antibodies directed against the target antigen can be separated from contaminating antibodies by affinity purification. For most purposes, antisera need not be fractionated before use. If the antisera are to be radiolabeled or conjugated to enzymes such as horseradish peroxidase (HRP) or alkali ...
... 3) Antibodies directed against the target antigen can be separated from contaminating antibodies by affinity purification. For most purposes, antisera need not be fractionated before use. If the antisera are to be radiolabeled or conjugated to enzymes such as horseradish peroxidase (HRP) or alkali ...