Download ProteinChipâ technology is one of the most exciting advancements

ProteinChip® Technology: Applications and Perspectives
Alexander Karavanov
Ciphergen Biosystems, Inc., Fremont, CA, USA
ProteinChip technology is one of the most exciting advancements in protein analysis in the
last 5 years. The Protein Biology SystemTM (PBS) combines the power of mass analysis with
chromatography surfaces on an integrated platform. The PBS can easily be used by biologists,
biochemists, and clinicians. ProteinChip uses a wide variety of surface chemistries (normal
phase, reverse phase, ion exchange, metal ions, and more, including surfaces designed by the
user) to facilitate analysis of complex biological systems. This allows the use of retention
chromatography to capture, purify, characterize and identify proteins of interest.
ProteinChip allows the use of antibodies, DNA, proteins, lectins and many other biologically
defined surfaces for capture and analysis of proteins
from complex mixtures: serum, urine,
cell and nuclear extracts etc. Its applications include: biomarker discovery and characterization,
protein- protein interactions, protein modification analysis, protein identification and many
others.
Short review of existing and emerging protein array technologies along with the data obtained
by ProteinChip technology would be presented.
The latter includes protein profiling, protein-protein interactions, glycosylation analysis and
protein identification.