Understanding The Function And Regulation Of Eukaryotic Release
... Several studies have tried to address the unanswered question of how a protein (RF1 or RF2) recognizes a codon instead of the usual tRNA. Initial studies suggested that prokaryotic RFs recognize each stop codon through a tripeptide “anticodon” located in domain 2 of each RF. The PxT and SPF motifs w ...
... Several studies have tried to address the unanswered question of how a protein (RF1 or RF2) recognizes a codon instead of the usual tRNA. Initial studies suggested that prokaryotic RFs recognize each stop codon through a tripeptide “anticodon” located in domain 2 of each RF. The PxT and SPF motifs w ...
Sample pages 1 PDF
... The investigation of plant myosin had begun from attempting to elucidate the molecular mechanism of characean cytoplasmic streaming [82]. In 1970–1990, several proteins with ATPase activities had been isolated biochemically and reported as plant myosins (cited in [84]). However, none of these result ...
... The investigation of plant myosin had begun from attempting to elucidate the molecular mechanism of characean cytoplasmic streaming [82]. In 1970–1990, several proteins with ATPase activities had been isolated biochemically and reported as plant myosins (cited in [84]). However, none of these result ...
Actin as target for modification by bacterial protein toxins
... Fig. 2. Different structures of actin–ADP-ribosylating toxins ⁄ effectors, which all modify actin at Arg177. The family of binary toxins consists of Clostridium botulinum C2 toxin, Clostridium perfringens iota toxin, Clostridium difficile transferase (CDT), Bacillus cereus vegetative insecticidal to ...
... Fig. 2. Different structures of actin–ADP-ribosylating toxins ⁄ effectors, which all modify actin at Arg177. The family of binary toxins consists of Clostridium botulinum C2 toxin, Clostridium perfringens iota toxin, Clostridium difficile transferase (CDT), Bacillus cereus vegetative insecticidal to ...
Gene Section PML (promyelocytic leukemia) Atlas of Genetics and Cytogenetics
... The RING domain of PML is involved in the formation of the PML nuclear bodies (PML-NBs, see below) and in several others PML functions. Adjacent to the RING domain lay two cysteine-rich domains named B-boxes: these two domains have been proposed to work as second zinc-binding domain and they are inv ...
... The RING domain of PML is involved in the formation of the PML nuclear bodies (PML-NBs, see below) and in several others PML functions. Adjacent to the RING domain lay two cysteine-rich domains named B-boxes: these two domains have been proposed to work as second zinc-binding domain and they are inv ...
Functional analysis of parvin and different modes of IPP
... (CH) domains separated by a linker sequence, and a less-well conserved N-terminal region. In vivo structure–function analysis revealed that all the domains are essential for parvin function, whereas recruitment at integrin adhesion sites is mediated by two localization signals: one located within th ...
... (CH) domains separated by a linker sequence, and a less-well conserved N-terminal region. In vivo structure–function analysis revealed that all the domains are essential for parvin function, whereas recruitment at integrin adhesion sites is mediated by two localization signals: one located within th ...
Lipoprotein structure
... cholesterol-to-phospholipid ratio in LDL (M.C. Phillips, 1989). The surface monolayer of VLDL is even more fluid than that of HDL due to differences in the monolayer lipids. Apolipoproteins exert little or no effect on the average dynamic properties of the surface lipids especially in LDL and VLDL. ...
... cholesterol-to-phospholipid ratio in LDL (M.C. Phillips, 1989). The surface monolayer of VLDL is even more fluid than that of HDL due to differences in the monolayer lipids. Apolipoproteins exert little or no effect on the average dynamic properties of the surface lipids especially in LDL and VLDL. ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... non-native substrate. (A) Detection of complexes between Hsp70, BAG-1 and RCMLA by gel sieving chromatography. Reaction mixtures containing 14 μM GST-BAG-1 ⫹ 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (s), 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (u), or 2.8 μM [125I]RCMLA alone (e) were incubated and loaded on the S ...
... non-native substrate. (A) Detection of complexes between Hsp70, BAG-1 and RCMLA by gel sieving chromatography. Reaction mixtures containing 14 μM GST-BAG-1 ⫹ 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (s), 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (u), or 2.8 μM [125I]RCMLA alone (e) were incubated and loaded on the S ...
Annexin-Phospholipid Interactions. Functional Implications
... conserved core composed of four (eight in annexin A6) homologous domains of about 70 amino acids showing a similar three-dimensional structure highly conserved throughout annexin evolution [6]. Inside each domain, a sequence of 17 amino acid residues is found which presents an even higher degree of ...
... conserved core composed of four (eight in annexin A6) homologous domains of about 70 amino acids showing a similar three-dimensional structure highly conserved throughout annexin evolution [6]. Inside each domain, a sequence of 17 amino acid residues is found which presents an even higher degree of ...
phosphoinositides: tiny lipids with giant impact on cell regulation
... Although individual groups of PPI metabolizing enzymes will be featured in more details below, a few important general questions are worth highlighting here. The first is related to their substrate recognition. Most, if not all, of the PPI kinase and phosphatase enzymes are loosely membrane-associat ...
... Although individual groups of PPI metabolizing enzymes will be featured in more details below, a few important general questions are worth highlighting here. The first is related to their substrate recognition. Most, if not all, of the PPI kinase and phosphatase enzymes are loosely membrane-associat ...
Collagen Diversity, Synthesis and Assembly
... cross-links; recent studies have shown how flexibility within the non-collagenous domains allows these cross-links to form (Eyre et al., 2004). Strongest homology within the FACITs is in the C-terminal collagenous (COL1) and non-collagenous (NC1) domains, and all have a thrombospondin N-terminal-lik ...
... cross-links; recent studies have shown how flexibility within the non-collagenous domains allows these cross-links to form (Eyre et al., 2004). Strongest homology within the FACITs is in the C-terminal collagenous (COL1) and non-collagenous (NC1) domains, and all have a thrombospondin N-terminal-lik ...
Euglena gracilis ascorbate peroxidase forms an intramolecular
... catalytic domains. Computer-assisted comparison of the deduced amino acid sequences of APX-N and APX-C with sequences in the database revealed approx. 38–54 % sequence identity with APX proteins from various species. The phylogenetic relationships of various APX proteins, including Euglena APX, were ...
... catalytic domains. Computer-assisted comparison of the deduced amino acid sequences of APX-N and APX-C with sequences in the database revealed approx. 38–54 % sequence identity with APX proteins from various species. The phylogenetic relationships of various APX proteins, including Euglena APX, were ...
The Plant Cell - Department of Biology
... binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion protein is predicted to produce a polypeptide of 33 kD. The expressed proteins were isolated and a polypeptide o ...
... binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion protein is predicted to produce a polypeptide of 33 kD. The expressed proteins were isolated and a polypeptide o ...
Discrete Domains Mediate the Light-Responsive
... The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical role in the repression of photomorphogenesis during Arabidopsis seedling development. We investigated the control of COP1 partitioning between nucleus and cytoplasm, which has been implicated in the regulation of COP1 act ...
... The Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) protein plays a critical role in the repression of photomorphogenesis during Arabidopsis seedling development. We investigated the control of COP1 partitioning between nucleus and cytoplasm, which has been implicated in the regulation of COP1 act ...
FUNCTIONAL CONSEQUENCES OF THE DIRECT PHYSICAL CBP/p300
... of his lab. He is very enthusiastic about research and I have learned a great deal from him. David, I greatly appreciate your mentorship during the course of my PhD. ...
... of his lab. He is very enthusiastic about research and I have learned a great deal from him. David, I greatly appreciate your mentorship during the course of my PhD. ...
Interactions of Annexins with the mu Subunits of the Clathrin
... might therefore function to initiate calcium-regulated coated pit formation at the cell surface or on intracellular organelles. ...
... might therefore function to initiate calcium-regulated coated pit formation at the cell surface or on intracellular organelles. ...
Linear ubiquitin chains: enzymes, mechanisms and biology
... The HOIP subunit of LUBAC contains all the catalytic machinery required to synthesize linear ubiquitin chains with high specificity [2,3]. This activity is located in its C-terminal portion within the RBR domain plus a C-terminal extension referred to as the linear ubiquitin chain-determining domain ...
... The HOIP subunit of LUBAC contains all the catalytic machinery required to synthesize linear ubiquitin chains with high specificity [2,3]. This activity is located in its C-terminal portion within the RBR domain plus a C-terminal extension referred to as the linear ubiquitin chain-determining domain ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.