Role of hsp90 and the hsp90-binding immunophilins in signalling
... and cyclosporine A. All members of the immunophilin family have peptidylprolyl isomerase (PPIase) activity, and they are divided into two classes: the FKBPs bind FK506 and rapamycin, and the cyclophilins (CyPs) bind cyclosporine A. The immunosuppressant drugs occupy the PPIase site on the immunophil ...
... and cyclosporine A. All members of the immunophilin family have peptidylprolyl isomerase (PPIase) activity, and they are divided into two classes: the FKBPs bind FK506 and rapamycin, and the cyclophilins (CyPs) bind cyclosporine A. The immunosuppressant drugs occupy the PPIase site on the immunophil ...
AraC Protein, Regulation of the L-arabinose Operon in Escherichia
... generates an intricate network of hydrogen bonds between arabinose and residues lining the binding pocket (Soisson et al., 1997). Analysis of the structure allowed Tang et al. (2008) to identify those residues most critical to the stereospecific binding of L-arabinose vs. D-arabinose. Upon randomiza ...
... generates an intricate network of hydrogen bonds between arabinose and residues lining the binding pocket (Soisson et al., 1997). Analysis of the structure allowed Tang et al. (2008) to identify those residues most critical to the stereospecific binding of L-arabinose vs. D-arabinose. Upon randomiza ...
structure-function analysis of the drosophila stubble type ii
... The catalytic activity of TTSPs is dependent on the presence of three residues in the proteolytic domain, histidine, aspartate, and serine, which make up the catalytic triad. Enzymatic activity is further modulated by the structural organization of the substrate binding pocket which is responsible f ...
... The catalytic activity of TTSPs is dependent on the presence of three residues in the proteolytic domain, histidine, aspartate, and serine, which make up the catalytic triad. Enzymatic activity is further modulated by the structural organization of the substrate binding pocket which is responsible f ...
A C-terminus Mitochondrial-localization Region and BH3 Domain of
... Merwin, Liz A., "A C-terminus Mitochondrial-localization Region and BH3 Domain of Puma are Required for Apoptotic Function" (2013). Electronic Thesis and Dissertation Repository. Paper 1283. ...
... Merwin, Liz A., "A C-terminus Mitochondrial-localization Region and BH3 Domain of Puma are Required for Apoptotic Function" (2013). Electronic Thesis and Dissertation Repository. Paper 1283. ...
Structural studies on lysosomal proteins
... the protein structure in a way that cannot be compensated by other amino acids. Enzyme enhancement therapy with chemical chaperones is a novel treatment for LSDs and has shown potential also for Pompe disease. In this study, the stabilisation capacity of potential chemical chaperones for GAA were te ...
... the protein structure in a way that cannot be compensated by other amino acids. Enzyme enhancement therapy with chemical chaperones is a novel treatment for LSDs and has shown potential also for Pompe disease. In this study, the stabilisation capacity of potential chemical chaperones for GAA were te ...
Troubleshooting SDS-PAGE-0410
... There are no net negative charges on proteins, the protein will not move down the gel, ensure SDS has been added to the sample. The solution is acidic, add NaOH until the solution turns blue. There is too little bromophenol blue in the sample buffer. The buffers are too concentrated, dilute the buff ...
... There are no net negative charges on proteins, the protein will not move down the gel, ensure SDS has been added to the sample. The solution is acidic, add NaOH until the solution turns blue. There is too little bromophenol blue in the sample buffer. The buffers are too concentrated, dilute the buff ...
Calcium binding chaperones of the endoplasmic reticulum
... (Barnes and Smoak 1997). It has been found that the selective increase in Grp94 in response to Ca2+ levels protects cardiomyocytes in ischemia (Vitadello et al. 2003). There are several ER oxidoreducatases in the ER lumen that also have roles buffering ER Ca2+. PDI is a 58-kDa protein that binds Ca2 ...
... (Barnes and Smoak 1997). It has been found that the selective increase in Grp94 in response to Ca2+ levels protects cardiomyocytes in ischemia (Vitadello et al. 2003). There are several ER oxidoreducatases in the ER lumen that also have roles buffering ER Ca2+. PDI is a 58-kDa protein that binds Ca2 ...
Termination of translation: interplay of mRNA, rRNAs and release
... Secondly, the stop codon is in a close contact with rRNA within the ribosome (see Poole and Tate, 2000; Chavatte et al., 2001, 2002). Thirdly, two segments of prokaryotic 23S rRNA (helices 69 and 89) can be folded into a tRNAlike shape with anticodon-like loops complementary to stop codons (Ivanov e ...
... Secondly, the stop codon is in a close contact with rRNA within the ribosome (see Poole and Tate, 2000; Chavatte et al., 2001, 2002). Thirdly, two segments of prokaryotic 23S rRNA (helices 69 and 89) can be folded into a tRNAlike shape with anticodon-like loops complementary to stop codons (Ivanov e ...
Functions of the cytoplasmic domain of the βPS
... allow extensive cellular rearrangements to take place. Several adhesive components (reviewed in Hynes and Lander, 1992) are shared between vertebrates and invertebrates, among them the large family of integrin proteins (Albelda and Buck, 1990; Hemler, 1990; Hynes, 1992). These integral membrane prot ...
... allow extensive cellular rearrangements to take place. Several adhesive components (reviewed in Hynes and Lander, 1992) are shared between vertebrates and invertebrates, among them the large family of integrin proteins (Albelda and Buck, 1990; Hemler, 1990; Hynes, 1992). These integral membrane prot ...
Article - Andrej Sali
... between them (Figure 4C). Protein S-VIII may also interact with ES3S. In addition, some unmodeled density between the rods (see red sphere, Figure 4C) may correspond to ES1S, which forms a bulge, and some additional protein. In the large subunit, the L-XII protein density contains two long a helices ...
... between them (Figure 4C). Protein S-VIII may also interact with ES3S. In addition, some unmodeled density between the rods (see red sphere, Figure 4C) may correspond to ES1S, which forms a bulge, and some additional protein. In the large subunit, the L-XII protein density contains two long a helices ...
REVIEWS Structural insights into the function of the
... Deletion of even a few residues at the a sequential 13-strand, an a-helix and a the cell. carboxyl terminus would be predicted [3-strand (referred to as the 13al3 unit). Flavoproteins catalyse a broad specto disrupt the tight hydrophobic pack- This [3~[3 unit contains a consensus trum of redox proce ...
... Deletion of even a few residues at the a sequential 13-strand, an a-helix and a the cell. carboxyl terminus would be predicted [3-strand (referred to as the 13al3 unit). Flavoproteins catalyse a broad specto disrupt the tight hydrophobic pack- This [3~[3 unit contains a consensus trum of redox proce ...
enterocolitica Yersinia Type III Secretion System of Properties of the
... particularly marked in their C-terminal halves. Deletion mutagenesis (20) and limited proteolysis (4, 43) have shown that this domain participates in oligomerization and pore formation, while the N terminus is probably involved in systemspecific interactions (12, 49). A small nonconserved domain C t ...
... particularly marked in their C-terminal halves. Deletion mutagenesis (20) and limited proteolysis (4, 43) have shown that this domain participates in oligomerization and pore formation, while the N terminus is probably involved in systemspecific interactions (12, 49). A small nonconserved domain C t ...
Introduction - ART
... functions in eukaryotes through its covalent conjugation to other intracellular proteins. Substrates marked with a polymer of ubiquitins (a polyubiquitin chain) are selectively targeted to a multisubunit ATP-dependent protease known as the 26 proteasome (Hershko et al., 1998), whereas certain substr ...
... functions in eukaryotes through its covalent conjugation to other intracellular proteins. Substrates marked with a polymer of ubiquitins (a polyubiquitin chain) are selectively targeted to a multisubunit ATP-dependent protease known as the 26 proteasome (Hershko et al., 1998), whereas certain substr ...
Glucansucrases: mechanism of action and structure–function
... Glucansucrases are produced principally by Leuconostoc mesenteroides and oral Streptococcus species, but also by the lactic acid bacteria (Lactococci, Lactobacilli). They catalyse the synthesis of high molecular weight D-glucose polymers, named glucans, from sucrose. In the presence of efficient acc ...
... Glucansucrases are produced principally by Leuconostoc mesenteroides and oral Streptococcus species, but also by the lactic acid bacteria (Lactococci, Lactobacilli). They catalyse the synthesis of high molecular weight D-glucose polymers, named glucans, from sucrose. In the presence of efficient acc ...
Current Status and Future Prospectives of C1 Domain Ligands as
... ligands have been characterized are mainly based on the proposed role of PKC in the pathophysiology of cancer and AD. In cancer, the differential effects of PKC isoforms on cell proliferation and apoptosis form the basis for targeting PKC. In the central nervous system, PKC regulates processes linke ...
... ligands have been characterized are mainly based on the proposed role of PKC in the pathophysiology of cancer and AD. In cancer, the differential effects of PKC isoforms on cell proliferation and apoptosis form the basis for targeting PKC. In the central nervous system, PKC regulates processes linke ...
Facing extremes: archaeal surface-layer (glyco)proteins
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
... survival in the face of such environmental challenges. One aspect of such investigation asks how the archaeal cell envelope, directly exposed to the harsh physical conditions in which these micro-organisms exist, manages to maintain its structural integrity. In most cases, a surface (S)-layer, gener ...
Hedgehog signal transduction: recent findings Kent
... Shh-signaling pathway could play in Shh sequestration and/or reception in vertebrate tissues. Both Hip1 and GAS-1 appear to be involved in sequestering Shh and attenuating its signaling in Shh-responsive cells. This attenuation of Shh signaling is speculatively illustrated as Hip1 and GAS-1 binding ...
... Shh-signaling pathway could play in Shh sequestration and/or reception in vertebrate tissues. Both Hip1 and GAS-1 appear to be involved in sequestering Shh and attenuating its signaling in Shh-responsive cells. This attenuation of Shh signaling is speculatively illustrated as Hip1 and GAS-1 binding ...
Phosphorylation of apoproteins in VLDL, and LDL by protein
... for apo BlOO phosphorylated by CAMP-PKcompared with AMPPK (Figure lb). This indicates that these two kinases phosphorylate different sites. This has also been suggested by reversed phase HPLC separation of tryptic peptides from apo BlOO phosphorylated by either kinase and will be confmed when these ...
... for apo BlOO phosphorylated by CAMP-PKcompared with AMPPK (Figure lb). This indicates that these two kinases phosphorylate different sites. This has also been suggested by reversed phase HPLC separation of tryptic peptides from apo BlOO phosphorylated by either kinase and will be confmed when these ...
Protein domain
A protein domain is a conserved part of a given protein sequence and (tertiary) structure that can evolve, function, and exist independently of the rest of the protein chain. Each domain forms a compact three-dimensional structure and often can be independently stable and folded. Many proteins consist of several structural domains. One domain may appear in a variety of different proteins. Molecular evolution uses domains as building blocks and these may be recombined in different arrangements to create proteins with different functions. Domains vary in length from between about 25 amino acids up to 500 amino acids in length. The shortest domains such as zinc fingers are stabilized by metal ions or disulfide bridges. Domains often form functional units, such as the calcium-binding EF hand domain of calmodulin. Because they are independently stable, domains can be ""swapped"" by genetic engineering between one protein and another to make chimeric proteins.