PDF - Beilstein
... commonly associated with NRPSs rather than RiPPs, but the function of these small LanBs is not yet known [52,53]. In contrast to class I lanthipeptides, both dehydration and cyclisation reactions are catalysed by bifunctional lanthionine synthetases for classes II–IV [47,49,54]. Furthermore, dehydra ...
... commonly associated with NRPSs rather than RiPPs, but the function of these small LanBs is not yet known [52,53]. In contrast to class I lanthipeptides, both dehydration and cyclisation reactions are catalysed by bifunctional lanthionine synthetases for classes II–IV [47,49,54]. Furthermore, dehydra ...
Investigations of non-enzymatic glycation with new bioanalytical
... Desalting of proteins and peptides by solid phase extraction (SPE) Solid phase extraction (SPE) is an easily applicable method most frequently used for desalting, purification, preconcentration and fractionation of a complex mixture of peptides. Fractionation prior to analyses can have a significant ...
... Desalting of proteins and peptides by solid phase extraction (SPE) Solid phase extraction (SPE) is an easily applicable method most frequently used for desalting, purification, preconcentration and fractionation of a complex mixture of peptides. Fractionation prior to analyses can have a significant ...
Ph.D. Thesis_Erban_Tomas
... of stored products or parasites (Montealegre et al. 2002). Synanthropic acaridid mite species are usually classified into two artificial groups: (1) house dust mites (HDMs) and (2) stored product mites (SPMs). Both groups are of high medical and economic importance (Hughes 1976, Colloff 2009). Both ...
... of stored products or parasites (Montealegre et al. 2002). Synanthropic acaridid mite species are usually classified into two artificial groups: (1) house dust mites (HDMs) and (2) stored product mites (SPMs). Both groups are of high medical and economic importance (Hughes 1976, Colloff 2009). Both ...
The Copper Efflux Regulator CueR Is Subject to ATP
... To analyze the stability of different CueR variants, cells containing inducible expression plasmids encoding for corresponding CueR proteins were grown overnight in M9 minimal medium containing corresponding antibiotics for selection at 30◦ C. Fifteen milliliters of M9 minimal medium supplemented wi ...
... To analyze the stability of different CueR variants, cells containing inducible expression plasmids encoding for corresponding CueR proteins were grown overnight in M9 minimal medium containing corresponding antibiotics for selection at 30◦ C. Fifteen milliliters of M9 minimal medium supplemented wi ...
Keyhole Limpet Hemocyanin ( KLH )
... immunization applications, theses haptens are conjugated to large molecules (carriers) that aid to stimulate an immune response. The carrier-hapten conjugate carry many epitopes to which T lymphocytes can respond and subsequently provide the necessary help for antibody-producing B cells. Confering a ...
... immunization applications, theses haptens are conjugated to large molecules (carriers) that aid to stimulate an immune response. The carrier-hapten conjugate carry many epitopes to which T lymphocytes can respond and subsequently provide the necessary help for antibody-producing B cells. Confering a ...
Aeromonas chitinase degrades chironomid egg masses
... (1999). According to this assay, a specific intergenic region between the 16S rRNA and the 23S rRNA genes was replicated. Isolates that were found positive belonged to the Aeromonas genus. For species identification, the rpoD gene (RNA polymerase that encodes subunit D) was amplified and sequenced a ...
... (1999). According to this assay, a specific intergenic region between the 16S rRNA and the 23S rRNA genes was replicated. Isolates that were found positive belonged to the Aeromonas genus. For species identification, the rpoD gene (RNA polymerase that encodes subunit D) was amplified and sequenced a ...
Antimicrobial peptides in crustaceans
... identified and are accessible on databases (http://aps.unmc.edu/AP/main.php) or in journal publications (see for review Thomas et al., 2010). AMPs are classically described as small cationic, amphipathic, gene-encoded molecules (<10 kDa, 15-100 amino acids) that differ considerably in amino acid seq ...
... identified and are accessible on databases (http://aps.unmc.edu/AP/main.php) or in journal publications (see for review Thomas et al., 2010). AMPs are classically described as small cationic, amphipathic, gene-encoded molecules (<10 kDa, 15-100 amino acids) that differ considerably in amino acid seq ...
EC 3.4.22.32 - Bio
... The mechanism of the putative anti-inflammatory activity is not well understood. It may be accounted for, in part, by activation of plasmin production from plasminogen and reduction of kinin, via inhibition of the conversion of kininogen to kinin. Other possibilities, include proteolytic degradation ...
... The mechanism of the putative anti-inflammatory activity is not well understood. It may be accounted for, in part, by activation of plasmin production from plasminogen and reduction of kinin, via inhibition of the conversion of kininogen to kinin. Other possibilities, include proteolytic degradation ...
THE ESTIMATION OF ACTIVE NATIVE TRYPSIN IN THE
... denatured hemoglobin by trypsin is allowed to take place in a slightly alkaline solution buffered with phosphate. Precipitation of the denatured hemoglobin is prevented by urea. The undigested hemoglobin is precipitated with trichloracetic acid. The digested hemoglobin not precipitated by trichlorac ...
... denatured hemoglobin by trypsin is allowed to take place in a slightly alkaline solution buffered with phosphate. Precipitation of the denatured hemoglobin is prevented by urea. The undigested hemoglobin is precipitated with trichloracetic acid. The digested hemoglobin not precipitated by trichlorac ...
ldentification of Surface-Exposed Domains on the Reducing Side of
... and PsaF that are accessible to proteases and N-hydroxysuccinimidobiotin (NHS-biotin). Upon exposure of PSI complexes to a low concentration of endoproteinase glutamic acid (CIu)-C, PsaE was cleaved to 7.1- and 6.6-kD N-terminal fragments without significant cleavage of other subunits. C W and Clu6' ...
... and PsaF that are accessible to proteases and N-hydroxysuccinimidobiotin (NHS-biotin). Upon exposure of PSI complexes to a low concentration of endoproteinase glutamic acid (CIu)-C, PsaE was cleaved to 7.1- and 6.6-kD N-terminal fragments without significant cleavage of other subunits. C W and Clu6' ...
Outer Membrane Vesicle-Mediated Export of
... and PKD2) has not yet been fully investigated, although they have been suggested to be involved in protein-protein or protein-carbohydrate interaction [12]. Our previous studies provided a crystal structure model of the PKD1 domain from V. cholerae PrtV (residues 755– 838) and revealed a Ca++-bindin ...
... and PKD2) has not yet been fully investigated, although they have been suggested to be involved in protein-protein or protein-carbohydrate interaction [12]. Our previous studies provided a crystal structure model of the PKD1 domain from V. cholerae PrtV (residues 755– 838) and revealed a Ca++-bindin ...
- Gastroenterology
... investigated whether cathepsin L (CTSL) can also process trypsinogen to active trypsin and has a role in pancreatitis. METHODS: In CTSL-deficient (Ctsl⫺/⫺) mice, pancreatitis was induced by injection of cerulein or infusion of taurocholate into the pancreatic duct. Human tissue, pancreatic juice, mo ...
... investigated whether cathepsin L (CTSL) can also process trypsinogen to active trypsin and has a role in pancreatitis. METHODS: In CTSL-deficient (Ctsl⫺/⫺) mice, pancreatitis was induced by injection of cerulein or infusion of taurocholate into the pancreatic duct. Human tissue, pancreatic juice, mo ...
The Calpain-Calpastatin System and the Calcium Paradox in the
... and 1 sodium pyruvate. The pH of the oxygenated KH buffer was adjusted at 42°C to 7.35-7.40. All KH buffers were equilibrated with 95% O2-5% CO2. In the calcium-free medium calcium was omitted and EGTA was added at a final concentration of 10 µM to ensure removal of any contaminant calcium. In diffe ...
... and 1 sodium pyruvate. The pH of the oxygenated KH buffer was adjusted at 42°C to 7.35-7.40. All KH buffers were equilibrated with 95% O2-5% CO2. In the calcium-free medium calcium was omitted and EGTA was added at a final concentration of 10 µM to ensure removal of any contaminant calcium. In diffe ...
Gloria Jih Biophysics 205 Semester Proposal Title: Global analysis
... CAD cells grown in media containing serum will be transfected with plasmids containing HAkinesin-1 or HA-kinesin-2. After transfection, cells will be induced to differentiate in media without serum, lysed and incubated with anti-HA antibody and subsequently with Protein-A sepharose. RNA will be extr ...
... CAD cells grown in media containing serum will be transfected with plasmids containing HAkinesin-1 or HA-kinesin-2. After transfection, cells will be induced to differentiate in media without serum, lysed and incubated with anti-HA antibody and subsequently with Protein-A sepharose. RNA will be extr ...
Epitope mapping of gliadin – a trigger of celiac disease
... Especially a deamidated peptide, which corresponds to amino acids 58-73 from the central domain induce celiac disease. This peptide contains the motif PQPELPY, which has been suggested to be an immunodominant epitope of gliadin ...
... Especially a deamidated peptide, which corresponds to amino acids 58-73 from the central domain induce celiac disease. This peptide contains the motif PQPELPY, which has been suggested to be an immunodominant epitope of gliadin ...
FEBS Letters 541, 176-177
... Hes1 is bound to DNA is kh /(kh +[Hes1]h ), where k is a characteristic concentration for dissociation of Hes1 from the DNA, and h is the Hill coe⁄cient that takes into account the cooperative character of the binding process. Moreover, Eq. 1 says that mRNA undergoes degradation with characteristic ...
... Hes1 is bound to DNA is kh /(kh +[Hes1]h ), where k is a characteristic concentration for dissociation of Hes1 from the DNA, and h is the Hill coe⁄cient that takes into account the cooperative character of the binding process. Moreover, Eq. 1 says that mRNA undergoes degradation with characteristic ...
Gel-based proteomics • Electrophoresis • One dimensional
... study structure and functions of proteins • Electrophoresis, based on principle of migration of charged proteins in an electric field • Gold standard due to its ability to provide information on protein structure and properties • Powerful technique for finer protein separation and visualization o ...
... study structure and functions of proteins • Electrophoresis, based on principle of migration of charged proteins in an electric field • Gold standard due to its ability to provide information on protein structure and properties • Powerful technique for finer protein separation and visualization o ...
Bax cleavage is mediated by calpain during drug-induced
... vitro by cysteine protease inhibitors including E-64 which distinguishes it from all known caspases and granzyme B, both of which are involved in apoptosis. Protease activity is also blocked by inhibitors against the calciumactivated neutral cysteine endopeptidase calpain. Partial puri®cation of the ...
... vitro by cysteine protease inhibitors including E-64 which distinguishes it from all known caspases and granzyme B, both of which are involved in apoptosis. Protease activity is also blocked by inhibitors against the calciumactivated neutral cysteine endopeptidase calpain. Partial puri®cation of the ...
Molecular Intercommunication between the Complement and
... fibrinolysis proteases may act as natural C3 and C5 convertases, generating biologically active anaphylatoxins, linking both cascades via multiple direct interactions in terms of a complex serine protease system. The Journal of Immunology, 2010, ...
... fibrinolysis proteases may act as natural C3 and C5 convertases, generating biologically active anaphylatoxins, linking both cascades via multiple direct interactions in terms of a complex serine protease system. The Journal of Immunology, 2010, ...
Signal Peptidases
... co-workers discovered that the light chain of IgG from myeloma cells was synthesized in a higher molecular weight form and was converted to its mature form when endoplasmic reticulum vesicles (microsomes) were added to the translation system.2 Milstein proposed a model based on these results in whic ...
... co-workers discovered that the light chain of IgG from myeloma cells was synthesized in a higher molecular weight form and was converted to its mature form when endoplasmic reticulum vesicles (microsomes) were added to the translation system.2 Milstein proposed a model based on these results in whic ...
Development of antifertility vaccine using sperm specific proteins
... murine testes caused complete impairment of fertility in in vitro fertilization (IVF) assay. However, active immunization studies showed significant reduction in fertility in majority but not in all the animals immunized. Another intra-acrosomal sperm antigen identified using monoclonal antibodies ...
... murine testes caused complete impairment of fertility in in vitro fertilization (IVF) assay. However, active immunization studies showed significant reduction in fertility in majority but not in all the animals immunized. Another intra-acrosomal sperm antigen identified using monoclonal antibodies ...
Purification and Properties of the Dictyostelium Calpain
... haploid, and established techniques are available for the generation, selection, and analysis of mutants that would be powerful tools to genetically establish calpain function (20). In this report, we have identified and isolated a calpain-like protein (Cpl) in Dictyostelium discoideum. The domain o ...
... haploid, and established techniques are available for the generation, selection, and analysis of mutants that would be powerful tools to genetically establish calpain function (20). In this report, we have identified and isolated a calpain-like protein (Cpl) in Dictyostelium discoideum. The domain o ...
The Right Whey Protein
... Lactose and milk fat may also vary per serving. Engineered whey protein isn’t cheap and expect to pay even more for a supreme blend. Combining different extraction methods costs more to manufacture and the taste and texture of the end product is difficult to attend. Choose a NAME BRAND when selectin ...
... Lactose and milk fat may also vary per serving. Engineered whey protein isn’t cheap and expect to pay even more for a supreme blend. Combining different extraction methods costs more to manufacture and the taste and texture of the end product is difficult to attend. Choose a NAME BRAND when selectin ...
Enzymes for Cell Dissociation and Lysis - Sigma
... activities Sigma-Aldrich tests each product lot for caseinase,14,15 clostripain and tryptic activities to evaluate the proteolytic activities in our collagenase products. The caseinase assay is the most important of the three for measuring the proteolytic activity that assists the digestion of anima ...
... activities Sigma-Aldrich tests each product lot for caseinase,14,15 clostripain and tryptic activities to evaluate the proteolytic activities in our collagenase products. The caseinase assay is the most important of the three for measuring the proteolytic activity that assists the digestion of anima ...
cDNA Library - +* Tudalenau Bangor Pages
... For probes to hybridise to their targets in a colony hybridisation, the probes have to be labelled (radioactive or non-radioactive) Two main labelling techniques random priming and nick translation: ...
... For probes to hybridise to their targets in a colony hybridisation, the probes have to be labelled (radioactive or non-radioactive) Two main labelling techniques random priming and nick translation: ...