PDF - Beilstein
... divided into four distinct classes (I–IV) based on the differences between the biosynthetic enzymes that carry out dehydration and cyclisation [44]. Dehydration in class I lanthipeptide pathways is catalysed by a LanB dehydratase (NisB for nisin) and cyclisation is catalysed by a zinc-dependent LanC ...
... divided into four distinct classes (I–IV) based on the differences between the biosynthetic enzymes that carry out dehydration and cyclisation [44]. Dehydration in class I lanthipeptide pathways is catalysed by a LanB dehydratase (NisB for nisin) and cyclisation is catalysed by a zinc-dependent LanC ...
43803_C000 1..20
... Growth Hormone and Athletic Performance ...........................................................................28 Problems with Exogenous Growth Hormone ..........................................................................30 Effects of Growth Hormone on Body Composition and Athletic Perform ...
... Growth Hormone and Athletic Performance ...........................................................................28 Problems with Exogenous Growth Hormone ..........................................................................30 Effects of Growth Hormone on Body Composition and Athletic Perform ...
Investigations of non-enzymatic glycation with new bioanalytical
... strength and pH of the binding buffer as well as the most appropriate circumstances of elutions. The results clearly demonstrate that the affinity tips bind the highest amount of glucose when the pH of the binding buffer varies between 7.7 and 8.2. Further enhancement of the pH of the binding buffer ...
... strength and pH of the binding buffer as well as the most appropriate circumstances of elutions. The results clearly demonstrate that the affinity tips bind the highest amount of glucose when the pH of the binding buffer varies between 7.7 and 8.2. Further enhancement of the pH of the binding buffer ...
Technical Brief
... This simple sentence, published in a narrative book chapter, not in a formal scientific journal, with little information on the dietary intake measure, and which was not supported by quantitative data nor statistical analysis, would not meet present criteria to be considered as solid evidence. Yet, ...
... This simple sentence, published in a narrative book chapter, not in a formal scientific journal, with little information on the dietary intake measure, and which was not supported by quantitative data nor statistical analysis, would not meet present criteria to be considered as solid evidence. Yet, ...
ASD v2.0: updated content and novel features
... Intrinsically, the allosteric regulation of proteins is triggered by the binding of a modulator to their allosteric sites; thus, it is useful to understand the knowledge of known allosteric sites involved in the allosteric regulation as well as the interactions between allosteric sites and modulator ...
... Intrinsically, the allosteric regulation of proteins is triggered by the binding of a modulator to their allosteric sites; thus, it is useful to understand the knowledge of known allosteric sites involved in the allosteric regulation as well as the interactions between allosteric sites and modulator ...
phosphoinositides: tiny lipids with giant impact on cell regulation
... the membranes and controlling vesicular trafficking and organelle physiology. The attention of scientists who study ion channels also turned toward PPIs as it became obvious that many channels or transporters require PPIs for their activity or control. The discovery of phosphatidylinositol 3-kinases ...
... the membranes and controlling vesicular trafficking and organelle physiology. The attention of scientists who study ion channels also turned toward PPIs as it became obvious that many channels or transporters require PPIs for their activity or control. The discovery of phosphatidylinositol 3-kinases ...
Purification and Characterization of Chorismate
... The purification of chorismate synthase starting from 50 g (wet weight) of E. gracilis cells is summarized in Table I. Chorismate synthase activity was not readily detectable in crude extracts of E. gracilis due to the presence of low molecular weight inhibitory material. To determine chorismate syn ...
... The purification of chorismate synthase starting from 50 g (wet weight) of E. gracilis cells is summarized in Table I. Chorismate synthase activity was not readily detectable in crude extracts of E. gracilis due to the presence of low molecular weight inhibitory material. To determine chorismate syn ...
MDM2 inhibit p300-mediated p53 acetylation and activation by
... Whether the inhibition requires the association of MDM2 with p53 compare wild-type MDM2 with its N-terminally deleted form lacking amino acid 58-89 (p53 binding domain) ...
... Whether the inhibition requires the association of MDM2 with p53 compare wild-type MDM2 with its N-terminally deleted form lacking amino acid 58-89 (p53 binding domain) ...
post- translational modifications
... Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs considerably increase the diversity of protein str ...
... Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs considerably increase the diversity of protein str ...
Novel control of lactate dehydrogenase from the freeze
... The importance of anaerobic glycolysis to cell, organ and whole animal survival during freezing led us to question whether the enzyme lactate dehydrogenase (LDH) was modified or regulated in any way that would support its role in freezing survival. LDH (EC 1.1.1.27) has been the subject of many stud ...
... The importance of anaerobic glycolysis to cell, organ and whole animal survival during freezing led us to question whether the enzyme lactate dehydrogenase (LDH) was modified or regulated in any way that would support its role in freezing survival. LDH (EC 1.1.1.27) has been the subject of many stud ...
CHAPTER 1 INTRODUCTION
... With the rising costs of energy, and limited availability of land greater emphasis on crop agriculture seems inevitable. In the future, plant proteins must provide an even greater proportion of our food protein. Cereals , as they traditionally have, may supply most of this, however , soya bean and, ...
... With the rising costs of energy, and limited availability of land greater emphasis on crop agriculture seems inevitable. In the future, plant proteins must provide an even greater proportion of our food protein. Cereals , as they traditionally have, may supply most of this, however , soya bean and, ...
Data mining for important amino acid residues in multiple sequence
... multiple subunits. Such complexes of two or more subunits are called multimers. Specifically, a complex with only one subunit is called a monomer, one with two ...
... multiple subunits. Such complexes of two or more subunits are called multimers. Specifically, a complex with only one subunit is called a monomer, one with two ...
Flux analysis in central carbon metabolism in plants
... I would like to thank Drs. Julie A. Dickerson (Electrical and Computer Engineering), Surya K. Mallapragada (Chemical Engineering), Martin H. Spalding (Genetics, Development, and Cell Biology), and R. Dennis Vigil (Chemical Engineering) for serving on my Program of Study committee. I am very thankful ...
... I would like to thank Drs. Julie A. Dickerson (Electrical and Computer Engineering), Surya K. Mallapragada (Chemical Engineering), Martin H. Spalding (Genetics, Development, and Cell Biology), and R. Dennis Vigil (Chemical Engineering) for serving on my Program of Study committee. I am very thankful ...
Regulation of Phenylalanine and Tyrosine
... extract (15 mg protein ml-l). After stirring for 20 min, the precipitate was collected by centrifugation (20000g, 20min) and dissolved in buffer A. The resulting protein solution (35 mgml-l) was dialysed for 2 h against 1000 vol. buffer A and stored at - 18 "C, if not used immediately. Sephadex G-10 ...
... extract (15 mg protein ml-l). After stirring for 20 min, the precipitate was collected by centrifugation (20000g, 20min) and dissolved in buffer A. The resulting protein solution (35 mgml-l) was dialysed for 2 h against 1000 vol. buffer A and stored at - 18 "C, if not used immediately. Sephadex G-10 ...
Transcription factor Sp3 is silenced through - IMT
... this lysine lies within a motif (IKEE, amino acids 422±425 according to Kingsley and Winoto, 1992) that could act as a target for SUMO modi®cation (Melchior, 2000). SUMO-modi®ed proteins have an ~20 kDa higher apparent molecular weight compared with unmodi®ed proteins. However, previously we did not ...
... this lysine lies within a motif (IKEE, amino acids 422±425 according to Kingsley and Winoto, 1992) that could act as a target for SUMO modi®cation (Melchior, 2000). SUMO-modi®ed proteins have an ~20 kDa higher apparent molecular weight compared with unmodi®ed proteins. However, previously we did not ...
Advances in Chemical Protein Modification
... nonselective protein modification methods, new chemical tools to access homogeneous proteins for in vitro and in vivo applications have been developed for the modification of natural Cys, Lys, and tyrosine (Tyr) residues. Selected examples of the next generation of Lys modifications are depicted in Sch ...
... nonselective protein modification methods, new chemical tools to access homogeneous proteins for in vitro and in vivo applications have been developed for the modification of natural Cys, Lys, and tyrosine (Tyr) residues. Selected examples of the next generation of Lys modifications are depicted in Sch ...
Biochemical characterization of the initial steps of the Kennedy
... Mops (pH 7.8), 6 mM MgCl2 and 5 mM ATP, with either 2 mM ethanolamine and 0.2 μCi of [3 H]ethanolamine or 2 mM choline and 0.2 μCi of [3 H]choline at 37 ◦C for 20 min and quenched by boiling at 100 ◦C for 5 min. Substrates and products were separated by HPTLC (high-performance TLC) using silica 60 p ...
... Mops (pH 7.8), 6 mM MgCl2 and 5 mM ATP, with either 2 mM ethanolamine and 0.2 μCi of [3 H]ethanolamine or 2 mM choline and 0.2 μCi of [3 H]choline at 37 ◦C for 20 min and quenched by boiling at 100 ◦C for 5 min. Substrates and products were separated by HPTLC (high-performance TLC) using silica 60 p ...
Characterization of the Two-Component, FAD-Dependent Monooxygenase SgcC That Requires Carrier Protein-Tethered
... an Amicon Ultra-4 concentrator device (5K, GE Healthcare) prior to use in SgcC assays. Determination of the Stereochemistry of (S)-3-Cl-β-tyrosylS-SgcC2 Substrate for SgcC. After the HiTrap Q anion-exchange column, the purified (S)-3-Cl-β-tyrosyl-S-SgcC2 (7) was subjected to alkaline hydrolysis in 0 ...
... an Amicon Ultra-4 concentrator device (5K, GE Healthcare) prior to use in SgcC assays. Determination of the Stereochemistry of (S)-3-Cl-β-tyrosylS-SgcC2 Substrate for SgcC. After the HiTrap Q anion-exchange column, the purified (S)-3-Cl-β-tyrosyl-S-SgcC2 (7) was subjected to alkaline hydrolysis in 0 ...
Glycation by Ascorbic Acid Causes Loss of Activity of Ribulose
... we used ascorbic acid (AsA) as a glycating agent and purified cucumber (Cucumis sativus L.) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a model protein in chloroplast tissues, and examined effects of glycation on the activity and susceptibility of Rubisco to proteases. Glycation pro ...
... we used ascorbic acid (AsA) as a glycating agent and purified cucumber (Cucumis sativus L.) ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) as a model protein in chloroplast tissues, and examined effects of glycation on the activity and susceptibility of Rubisco to proteases. Glycation pro ...
Supplemental Table 3
... A1, 2,6,6-trimethyl-1-(9'-hydroxy-3',7'-dimethylnona1',3',5',7'-tetraenyl)cyclohex-1-ene, one of the three components that makes up vitamin A. Retinol is an intermediate in the vision cycle and it also plays a role in growth and differentiation [goid 19841] [evidence IEA]; Interacting selectively wi ...
... A1, 2,6,6-trimethyl-1-(9'-hydroxy-3',7'-dimethylnona1',3',5',7'-tetraenyl)cyclohex-1-ene, one of the three components that makes up vitamin A. Retinol is an intermediate in the vision cycle and it also plays a role in growth and differentiation [goid 19841] [evidence IEA]; Interacting selectively wi ...
Of earwax and migration
... machines. For this purpose, pigments were bound with various ingredients such as honey, egg-tempera, gum-arabic and…earwax. As mentioned, cerumen is secreted by cerumen glands in the inner ear by way of biological pumps: the multiple drug resistance proteins. These are organic anion transporters whi ...
... machines. For this purpose, pigments were bound with various ingredients such as honey, egg-tempera, gum-arabic and…earwax. As mentioned, cerumen is secreted by cerumen glands in the inner ear by way of biological pumps: the multiple drug resistance proteins. These are organic anion transporters whi ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.