Enzymes responsible for chlorate reduction by Pseudomonas sp
... and 91% intact, respectively. Enzyme activity was larger in the periplasm fraction than in the cytoplasm fraction for chlorate and perchlorate, independent of whether cells were originally grown on chlorate or perchlorate. Based on these results, the periplasm fraction was selected for further analy ...
... and 91% intact, respectively. Enzyme activity was larger in the periplasm fraction than in the cytoplasm fraction for chlorate and perchlorate, independent of whether cells were originally grown on chlorate or perchlorate. Based on these results, the periplasm fraction was selected for further analy ...
Aeromonas chitinase degrades chironomid egg masses
... 48h. Each test was carried out at least three times. A reaction was considered positive when the egg mass structure disintegrated and a large excess of free eggs was found separated from the egg mass, as compared with the LB medium control. EMD activity of egg masses hatching prevention was also tes ...
... 48h. Each test was carried out at least three times. A reaction was considered positive when the egg mass structure disintegrated and a large excess of free eggs was found separated from the egg mass, as compared with the LB medium control. EMD activity of egg masses hatching prevention was also tes ...
C H A P
... the corresponding cell extract in lysis buffer. Purification of His-EstV from the cell extract was carried out by affinity chromatography using NTA-Ni resin, and the purified proteins were recovered and analyzed by SDS-PAGE and zymogram analysis before removing its His-tail. However, no properly pur ...
... the corresponding cell extract in lysis buffer. Purification of His-EstV from the cell extract was carried out by affinity chromatography using NTA-Ni resin, and the purified proteins were recovered and analyzed by SDS-PAGE and zymogram analysis before removing its His-tail. However, no properly pur ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
... Allophycocyanin, Linker, Anchor Protein, Phycobilisome Core, Mastigocladus laminosus Allophycocyanin (AP) was isolated from extracts of the cyanobacterium Mastigocladus laminosus. A fraction enriched in AP-associated polypeptides with apparent molecular masses of 2 1 -2 3 kD a in SDS-PAGE, was isola ...
... Allophycocyanin, Linker, Anchor Protein, Phycobilisome Core, Mastigocladus laminosus Allophycocyanin (AP) was isolated from extracts of the cyanobacterium Mastigocladus laminosus. A fraction enriched in AP-associated polypeptides with apparent molecular masses of 2 1 -2 3 kD a in SDS-PAGE, was isola ...
Functional characterization of the Mycobacterium
... primers pMALpknJFd and pMALpknJRv (Table S2) was ligated into vector pMAL-c2X (New England Biolabs) for overproduction of its gene product. The resulting ligation product, pMAL-c2X-pknJ, was transformed into E. coli BL21 Star (DE3) (Invitrogen) and the resulting recombinant protein was used for all ...
... primers pMALpknJFd and pMALpknJRv (Table S2) was ligated into vector pMAL-c2X (New England Biolabs) for overproduction of its gene product. The resulting ligation product, pMAL-c2X-pknJ, was transformed into E. coli BL21 Star (DE3) (Invitrogen) and the resulting recombinant protein was used for all ...
Bacterial Protein Synthesis Inhibitors (Antimicrobials)
... • This inhibition can take place at any stage in protein synthesis. • However, some inhibitors have a specific action in that they inhibit protein synthesis in prokaryotic cells but not in eukaryotic cells, or vice versa. prof. aza ...
... • This inhibition can take place at any stage in protein synthesis. • However, some inhibitors have a specific action in that they inhibit protein synthesis in prokaryotic cells but not in eukaryotic cells, or vice versa. prof. aza ...
The Major Component of the Paraflagellar Rod of Trypanosoma
... PR YPANOSOMA brucei, the causative agent of human sleeping sickness and a complex of veterinary diseases in large parts of Africa, is a uniflagellate protozoon (of the Kinetoplastida order and Trypanosomatida family). Its single flagellum, which arises from within the cell body, emerges through a "f ...
... PR YPANOSOMA brucei, the causative agent of human sleeping sickness and a complex of veterinary diseases in large parts of Africa, is a uniflagellate protozoon (of the Kinetoplastida order and Trypanosomatida family). Its single flagellum, which arises from within the cell body, emerges through a "f ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... primary export and re-export processes; (2) Mtr10, directly or indirectly, is responsible for the constitutive retrograde import of cytoplasmic tRNA to the nucleus; (3) Msn5 functions solely in the re-export process. In this thesis I focus on the physiological role(s) of the tRNA nuclear retrograde ...
... primary export and re-export processes; (2) Mtr10, directly or indirectly, is responsible for the constitutive retrograde import of cytoplasmic tRNA to the nucleus; (3) Msn5 functions solely in the re-export process. In this thesis I focus on the physiological role(s) of the tRNA nuclear retrograde ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
... bound activin is unable to initiate signal transduction and consequently follistatin is a potent antagonist of physiological activin signals. Of the three follistatin domains present in all follistatin isoforms, (Shimasaki et al., 1988) the first two, but not the third, are necessary for activin A b ...
METABOLIC CUES AND REGULATORY PROTEINS
... Table 2.S1. Implementation of the Philadelphia strain annotation ..................................57 Table 3.1. Bacterial strains and plasmids ......................................................................85 Table 3.2. Compounds that trigger premature differentiation in L. pneumophila ..... ...
... Table 2.S1. Implementation of the Philadelphia strain annotation ..................................57 Table 3.1. Bacterial strains and plasmids ......................................................................85 Table 3.2. Compounds that trigger premature differentiation in L. pneumophila ..... ...
The Vitamin K-dependent Carboxylase*
... How does the carboxylase recognize proteins in the secretion pathway as substrates for -carboxylation? In the case of the VKD blood proteins, there is now overwhelming evidence that carboxylase binds to an ≈ 18 amino acid (62) “propeptide” found on the amino-terminus of these substrates (Fig. 6). T ...
... How does the carboxylase recognize proteins in the secretion pathway as substrates for -carboxylation? In the case of the VKD blood proteins, there is now overwhelming evidence that carboxylase binds to an ≈ 18 amino acid (62) “propeptide” found on the amino-terminus of these substrates (Fig. 6). T ...
Developement of strategies for the isotopic labeling of methyl
... permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using this protocol, assignment of the methyl groups signals is mandatory. Two strategies were then propo ...
... permits the suppression of spurious correlations, especially harmful for structural studies based on detection/analysis of nOes. To make an efficient use of the obtained high quality NMR spectra using this protocol, assignment of the methyl groups signals is mandatory. Two strategies were then propo ...
The introduction and use of PKU sphere™, a Glycomacropeptide
... In the 1950’s, the first protein substitute (PS), which was based on protein hydrolysate, was developed to treat the first patient with PKU managed with diet therapy1. For the last 30 years, the most commonly used PS have been Phe-free L-amino acid supplements (L-AA supplements). They were originall ...
... In the 1950’s, the first protein substitute (PS), which was based on protein hydrolysate, was developed to treat the first patient with PKU managed with diet therapy1. For the last 30 years, the most commonly used PS have been Phe-free L-amino acid supplements (L-AA supplements). They were originall ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... The ribosomal stalk in bacteria is composed of four or six copies of L12 proteins arranged in dimers that bind to the adjacent sites on protein L10, spanning 10 amino acids each from the L10 C-terminus. To study why multiple L12 dimers are required on the ribosome, we created a chromosomally enginee ...
... The ribosomal stalk in bacteria is composed of four or six copies of L12 proteins arranged in dimers that bind to the adjacent sites on protein L10, spanning 10 amino acids each from the L10 C-terminus. To study why multiple L12 dimers are required on the ribosome, we created a chromosomally enginee ...
Cyclic-‐di-‐AMP: another second messenger enters the fray
... can be found in the Pfam database (Table S1)22. The majority of these proteins are found in bacterial species but a small number are also present in archaea within the Euryarchaeota ...
... can be found in the Pfam database (Table S1)22. The majority of these proteins are found in bacterial species but a small number are also present in archaea within the Euryarchaeota ...
LS1a Fall 2014 Lab 6: Ribosomal Protein Translation (PyMOL lab #3)
... of a sequence of ribonucleotides in a strand of messenger RNA (“mRNA”) into the correct sequence of amino acids of a protein. The ribosome reads the mRNA transcript in the 5’ 3’ direction, and synthesizes an amino acid polymer (a “polypeptide”) in the amino-terminal (“Nterminal”) to carboxy-termin ...
... of a sequence of ribonucleotides in a strand of messenger RNA (“mRNA”) into the correct sequence of amino acids of a protein. The ribosome reads the mRNA transcript in the 5’ 3’ direction, and synthesizes an amino acid polymer (a “polypeptide”) in the amino-terminal (“Nterminal”) to carboxy-termin ...
Advanced Stochastic Protein Sequence Analysis
... In the last decade(s), bioinformatics has become an impressive success story. Compared to traditional research in molecular biology, i.e. explorations in the so-called wet labs, insilico investigations are mostly cheaper and faster by some orders of magnitude. Here, the term in-silico stands for exp ...
... In the last decade(s), bioinformatics has become an impressive success story. Compared to traditional research in molecular biology, i.e. explorations in the so-called wet labs, insilico investigations are mostly cheaper and faster by some orders of magnitude. Here, the term in-silico stands for exp ...
Life in the slow lane: molecular mechanisms of estivation
... and Storey, 1989; Brooks and Storey, 1997). Suppression of oxidative metabolism was also indicated by cytochrome c oxidase (CCO) activities in mitochondria from hepatopancreas of estivating snails, Cepaea nemoralis, that were reduced by 84% (although total mitochondrial protein was unaffected) (Stua ...
... and Storey, 1989; Brooks and Storey, 1997). Suppression of oxidative metabolism was also indicated by cytochrome c oxidase (CCO) activities in mitochondria from hepatopancreas of estivating snails, Cepaea nemoralis, that were reduced by 84% (although total mitochondrial protein was unaffected) (Stua ...
Interactions of TCA cycle enzymes and of the CcpA
... Interaktion zwischen Mdh und Icd, welche ausschließlich durch die Substrate von Icd, die Kombination von Isocitrat, NADP+ und Mg2+, stimuliert wurde. Dieser stimulatorische Effekt ist hochspezifisch, da keine weiteren Metabolite und Kofaktoren von Icd und Mdh einen Einfluß auf die Interaktion von Md ...
... Interaktion zwischen Mdh und Icd, welche ausschließlich durch die Substrate von Icd, die Kombination von Isocitrat, NADP+ und Mg2+, stimuliert wurde. Dieser stimulatorische Effekt ist hochspezifisch, da keine weiteren Metabolite und Kofaktoren von Icd und Mdh einen Einfluß auf die Interaktion von Md ...
Marine Biotecnology
... chromatography using a FPLC system (Pharmacia Biotech, Uppsala, Sweden). Small proteins were removed by loading the sample onto a NAP-10TM Sephadex G25 column (Amersham Biosciences, Uppsala, Sweden) and eluted with 1.5 ml PBS buffer. The processed extracts were loaded into a 124-ml gel filtration co ...
... chromatography using a FPLC system (Pharmacia Biotech, Uppsala, Sweden). Small proteins were removed by loading the sample onto a NAP-10TM Sephadex G25 column (Amersham Biosciences, Uppsala, Sweden) and eluted with 1.5 ml PBS buffer. The processed extracts were loaded into a 124-ml gel filtration co ...
Purification and Biochemical Characterization of Digestive Lipase in
... chromatography using a FPLC system (Pharmacia Biotech, Uppsala, Sweden). Small proteins were removed by loading the sample onto a NAP-10TM Sephadex G25 column (Amersham Biosciences, Uppsala, Sweden) and eluted with 1.5 ml PBS buffer. The processed extracts were loaded into a 124-ml gel filtration co ...
... chromatography using a FPLC system (Pharmacia Biotech, Uppsala, Sweden). Small proteins were removed by loading the sample onto a NAP-10TM Sephadex G25 column (Amersham Biosciences, Uppsala, Sweden) and eluted with 1.5 ml PBS buffer. The processed extracts were loaded into a 124-ml gel filtration co ...
(1) Giycophorin was incorporated into large
... as a function of the lipid composition. (2) In agreement with previous data (Van der Steen, A.T.M., De Kruijff, B. and De Gier, J. (1982) Biochim. Biophys. Acta 691, 13-23) it was found that glycophorin greatly increased the bilayer permeability of DOPC vesicles. This effect was observed for a large ...
... as a function of the lipid composition. (2) In agreement with previous data (Van der Steen, A.T.M., De Kruijff, B. and De Gier, J. (1982) Biochim. Biophys. Acta 691, 13-23) it was found that glycophorin greatly increased the bilayer permeability of DOPC vesicles. This effect was observed for a large ...
Forced Expression of Dystrophin Deletion Constructs Reveals
... A. Cox is The Jackson Laboratories, 600 Main Street, Bar Harbor, ME ...
... A. Cox is The Jackson Laboratories, 600 Main Street, Bar Harbor, ME ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.