reprint - Charles Brenner Laboratory
... That which is needed to make sugars, building blocks and macromolecules is directed into anapleurotic and biosynthetic processes. That which can be oxidized in the citric acid cycle is consumed to generate ATP. In animals, all three classes of macronutrients have a storage form that protects against ...
... That which is needed to make sugars, building blocks and macromolecules is directed into anapleurotic and biosynthetic processes. That which can be oxidized in the citric acid cycle is consumed to generate ATP. In animals, all three classes of macronutrients have a storage form that protects against ...
Lactobacillus plantarum - UEF Electronic Publications
... fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, making the fermented product safe for the consumer. However, a product that has been fermented ...
... fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, making the fermented product safe for the consumer. However, a product that has been fermented ...
DOCTORAL THESIS
... shape. Plant cells treated with cell-wall degrading enzymes, which effectively removed the cell wall, are invariably spherical in contrast to the different cell shapes observed when the wall is present. Therefore the rigidity that defines cell morphology and plant morphology and structure are inheri ...
... shape. Plant cells treated with cell-wall degrading enzymes, which effectively removed the cell wall, are invariably spherical in contrast to the different cell shapes observed when the wall is present. Therefore the rigidity that defines cell morphology and plant morphology and structure are inheri ...
Multiple Lines of Evidence Localize Signaling
... organelle fraction of Arabidopsis protoplasts was carried out. This involved first obtaining a crude organelle fraction (referred to as the HSP) and then enriching mitochondria from this fraction, initially by density gradient centrifugation and subsequently by free-flow electrophoresis (FFE). The a ...
... organelle fraction of Arabidopsis protoplasts was carried out. This involved first obtaining a crude organelle fraction (referred to as the HSP) and then enriching mitochondria from this fraction, initially by density gradient centrifugation and subsequently by free-flow electrophoresis (FFE). The a ...
Using Mascot to characterise protein modifications
... • Arg can have mono or di methyl groups • Propyl amide can be on the N-terminus or on unmodified Lys or Lys with mono-methyl group • Mascot is one of the few software programs available for searching data with multiple modifications! ASMS 2003 The digestion of the H3 protein with Glu-C cleaved the p ...
... • Arg can have mono or di methyl groups • Propyl amide can be on the N-terminus or on unmodified Lys or Lys with mono-methyl group • Mascot is one of the few software programs available for searching data with multiple modifications! ASMS 2003 The digestion of the H3 protein with Glu-C cleaved the p ...
Modulation of functional properties of bifunctional S- Adenosylmethionine decarboxylase/Ornithine decarboxylase of
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
... Trishydroxy (methyl-amino) methane / Hydrochloric acid ...
Penagarikano O, Mulle JG, and Warren ST: The Pathophysiology of Fragile X Syndrome; Annual Rev. Genomics Hum. Genet., 8:109-29 (2007).
... markers known to flank the fragile site hybrid break points and showed that this YAC contains a CpG island aberrantly methylated in fragile X patients. Furthermore, these abnormally methylated fragments were unstable in affected individuals and increased in size when transmitted through a pedigree ( ...
... markers known to flank the fragile site hybrid break points and showed that this YAC contains a CpG island aberrantly methylated in fragile X patients. Furthermore, these abnormally methylated fragments were unstable in affected individuals and increased in size when transmitted through a pedigree ( ...
Chapter 2
... or product inhibition, and the ability to withstand components of the expected feedstock other than substrate. A team of scientists, engineers and accountants must then consider the next steps. If any of these parameters is unsatisfactory, the screen must continue until improved enzymes are located. ...
... or product inhibition, and the ability to withstand components of the expected feedstock other than substrate. A team of scientists, engineers and accountants must then consider the next steps. If any of these parameters is unsatisfactory, the screen must continue until improved enzymes are located. ...
Insights into interactions between poly(ethylene glycol) and proteins
... interactions between the measured species. It has been found that the second virial coefficient (B22) for the PEG - lysozyme interaction is lower (i.e. more favorable) than would be expected if the interaction was purely steric41-42. Such studies do not, however, provide atomic level detail to deter ...
... interactions between the measured species. It has been found that the second virial coefficient (B22) for the PEG - lysozyme interaction is lower (i.e. more favorable) than would be expected if the interaction was purely steric41-42. Such studies do not, however, provide atomic level detail to deter ...
Supporting document 1 Safety assessment
... properties of AHAS expressed in CV127. These studies demonstrated that the AHAS protein expressed in CV127 is as expected in terms of its physicochemical and functional properties but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal pept ...
... properties of AHAS expressed in CV127. These studies demonstrated that the AHAS protein expressed in CV127 is as expected in terms of its physicochemical and functional properties but the mature form of protein is slightly larger (by 34 amino acids) than anticipated due to the N-terminal signal pept ...
Appendix 1: PBS listings of Nutritional Products
... The PBS funds nutritional products for patients with metabolic conditions, infants and children with intolerance or allergy to cows’ milk protein, infants and children with lactose intolerance and other medical conditions requiring strict dietary management. Unlike registered medical products, infan ...
... The PBS funds nutritional products for patients with metabolic conditions, infants and children with intolerance or allergy to cows’ milk protein, infants and children with lactose intolerance and other medical conditions requiring strict dietary management. Unlike registered medical products, infan ...
Cell Division: The Place and Time of Cytokinesis Dispatch
... proceeds [9,10]. In Drosophila too, cycle 16 cells in pav mutants have a reduced level of microtubule bundling and show no cortical furrowing [11]. The new evidence reported by Somers and Saint [4] shows that the interaction between RacGAP50C and Pav is conserved in Drosophila. The mammalian ortholo ...
... proceeds [9,10]. In Drosophila too, cycle 16 cells in pav mutants have a reduced level of microtubule bundling and show no cortical furrowing [11]. The new evidence reported by Somers and Saint [4] shows that the interaction between RacGAP50C and Pav is conserved in Drosophila. The mammalian ortholo ...
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
... positions. The remaining 25 vertebrate sequences were aligned, of which a sampling of the most divergent representatives is given in Fig. 1, together with 2 plant and 2 yeast sequences. The plant and yeast sequences were the most closely related nonvertebrate aldo-keto reductases in the original dat ...
... positions. The remaining 25 vertebrate sequences were aligned, of which a sampling of the most divergent representatives is given in Fig. 1, together with 2 plant and 2 yeast sequences. The plant and yeast sequences were the most closely related nonvertebrate aldo-keto reductases in the original dat ...
World Index of BioMolecular Visualization Resources
... Cross-indexing terms: antibody; antibodies; multiple sequence alignment; sequence variability; homology modelling; immunoglobulin; domains; color-coded multiple sequence alignment. This site contains extensive tutorials and lectures on antibody structure coupled with analyses of sequence variability ...
... Cross-indexing terms: antibody; antibodies; multiple sequence alignment; sequence variability; homology modelling; immunoglobulin; domains; color-coded multiple sequence alignment. This site contains extensive tutorials and lectures on antibody structure coupled with analyses of sequence variability ...
MPN+, a putative catalytic motif found in a subset of MPN domain
... activity and a CSN-associated Nedd8/Rub1-removing activity. ...
... activity and a CSN-associated Nedd8/Rub1-removing activity. ...
Src protein–tyrosine kinase structure and regulation
... are non-receptor enzymes. The protein kinase family is the second largest enzyme family, and the fifth largest gene family in humans. It follows C2H2 zinc finger proteins (3%), G-protein coupled receptors (2.8%), the major histocompatibility complex protein family (2.8%), and the protease enzyme famil ...
... are non-receptor enzymes. The protein kinase family is the second largest enzyme family, and the fifth largest gene family in humans. It follows C2H2 zinc finger proteins (3%), G-protein coupled receptors (2.8%), the major histocompatibility complex protein family (2.8%), and the protease enzyme famil ...
12551_2008_5_MOESM1_ESM - Springer Static Content Server
... Filshie BK, Fraser RDB, MacRae TP et al (1964) X-ray diffraction and electronmicroscope observations on soluble derivatives of feather keratin. Biochem J 92: 19 A cross- conformation was observed using X-ray data obtained from oriented films of solubilised feather keratin. Electron microscope obser ...
... Filshie BK, Fraser RDB, MacRae TP et al (1964) X-ray diffraction and electronmicroscope observations on soluble derivatives of feather keratin. Biochem J 92: 19 A cross- conformation was observed using X-ray data obtained from oriented films of solubilised feather keratin. Electron microscope obser ...
Reprint
... All eRF3 proteins described so far contain a variable N terminal domain [37]. Its primary function is unlikely to be to catalyze prion formation, since such a property has not been described for eRF3 in species other than S. cerevisiae. However, this domain is not essential for cell viability in yea ...
... All eRF3 proteins described so far contain a variable N terminal domain [37]. Its primary function is unlikely to be to catalyze prion formation, since such a property has not been described for eRF3 in species other than S. cerevisiae. However, this domain is not essential for cell viability in yea ...
PPTX - Bonham Chemistry
... mineral salts like calcium sulfate (gypsum) or calcium chloride can be added to the brewing water to lower mash pH. Also, food grade acids , such as lactic acid or phosphoric acid, can be added to brewing water to lower mash pH. • The enzyme beta glucanase is active during this rest. This enzyme bre ...
... mineral salts like calcium sulfate (gypsum) or calcium chloride can be added to the brewing water to lower mash pH. Also, food grade acids , such as lactic acid or phosphoric acid, can be added to brewing water to lower mash pH. • The enzyme beta glucanase is active during this rest. This enzyme bre ...
Lactococcus lactis as expression host for the biosynthetic
... protein chemistry [1,2]. Its emission maximum (λmax ) and the quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligan ...
... protein chemistry [1,2]. Its emission maximum (λmax ) and the quantum yield (Q) are highly sensitive parameters for monitoring changes in the structure and dynamics of a protein. In numerous studies, the intrinsic protein fluorescence was used to monitor processes like enzyme kinetics, protein–ligan ...
The phosphopantetheinyl transferases
... Sfp expresses well in E. coli and other heterologous hosts and shows highly permissive catalytic activity towards CPs using not only CoA but CoA-like substrates. These properties now afforded many labs with the ability to delve deeply into the biosynthesis of many natural products. Until recently, Sf ...
... Sfp expresses well in E. coli and other heterologous hosts and shows highly permissive catalytic activity towards CPs using not only CoA but CoA-like substrates. These properties now afforded many labs with the ability to delve deeply into the biosynthesis of many natural products. Until recently, Sf ...
108. Regulation of the L-arabinose Operon in Escherichia coli, in
... or at least one of its two arms would have to be removed from a DNA-binding domain. Hence the arm–DNA-binding domain interactions make it energetically more favourable for the protein to engage in DNA-looping interactions between the distally located half-sites rather than utilize the adjacent half- ...
... or at least one of its two arms would have to be removed from a DNA-binding domain. Hence the arm–DNA-binding domain interactions make it energetically more favourable for the protein to engage in DNA-looping interactions between the distally located half-sites rather than utilize the adjacent half- ...
Identification of a Chloroplast-encoded 9-kDa
... mM Mes (pH6.5). 0.5% SDS, and 14 nmol of norleucine as an internal standard. Aliquots withdrawn at different times were acidified (pH 2.2) to stop the enzymatic reaction and used directly for amino acid analysis. Acid-labile Sulfideand Zero-valence Sulfur Acid-labilesulfide and zero-valencesulfurwer ...
... mM Mes (pH6.5). 0.5% SDS, and 14 nmol of norleucine as an internal standard. Aliquots withdrawn at different times were acidified (pH 2.2) to stop the enzymatic reaction and used directly for amino acid analysis. Acid-labile Sulfideand Zero-valence Sulfur Acid-labilesulfide and zero-valencesulfurwer ...
Identification of a Cis-Acting Element of ART1, a
... probe (STAR1-3, 2368 to 2288) was performed. STAR1-3 was able to be bound with ART1 protein and super shifted in the presence of anti-ART1 antibody (Fig. 2B). To further narrow the target region of STAR1 promoter, we divided STAR1-3 region into three parts (Fig. 1A, probes 1–3). Gel-shift assay show ...
... probe (STAR1-3, 2368 to 2288) was performed. STAR1-3 was able to be bound with ART1 protein and super shifted in the presence of anti-ART1 antibody (Fig. 2B). To further narrow the target region of STAR1 promoter, we divided STAR1-3 region into three parts (Fig. 1A, probes 1–3). Gel-shift assay show ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.