Detergents and Surfactants
... In general, biological detergents are most commonly used to disrupt the bipolar lipid membrane of cells in order to first free and then solubilize membrane-bound proteins. Some detergents can also be used to solubilize recombinant protein, while others find their usage in the stabilization, crystall ...
... In general, biological detergents are most commonly used to disrupt the bipolar lipid membrane of cells in order to first free and then solubilize membrane-bound proteins. Some detergents can also be used to solubilize recombinant protein, while others find their usage in the stabilization, crystall ...
MD1-103 Protein Stability Combo Kit
... RUBIC Buffer and RUBIC Additive Screens have been designed and developed by Stephane Boivin and Rob Meijers at the EMBL Hamburg and is manufactured exclusively under license by Molecular Dimensions Limited. Limited Use and Restrictions: Products sold by Molecular Dimensions Ltd. or its affiliates or ...
... RUBIC Buffer and RUBIC Additive Screens have been designed and developed by Stephane Boivin and Rob Meijers at the EMBL Hamburg and is manufactured exclusively under license by Molecular Dimensions Limited. Limited Use and Restrictions: Products sold by Molecular Dimensions Ltd. or its affiliates or ...
Nitrate reductase activity in chicory roots
... Detopping plantlets abolishes the shoot-to-root relationships. In the absence of sucrose, nitrate reduction is rapidly decreased (Brouquissee/o/., 1992). This resembles senescence in leaves. The decrease of enzymatic activities related to nitrogen assimilation and the subsequent increases of protein ...
... Detopping plantlets abolishes the shoot-to-root relationships. In the absence of sucrose, nitrate reduction is rapidly decreased (Brouquissee/o/., 1992). This resembles senescence in leaves. The decrease of enzymatic activities related to nitrogen assimilation and the subsequent increases of protein ...
Vanadium-Binding Proteins (Vanabins)
... vanadium(IV)(VO2+) and vanadium(V)([VO4]3-) ions. We used vanadium(IV) ions coordinated by iminodiacetic acid (IDA), because free vanadium(IV) ions precipitate at neutral pH. IDA was chosen because it does not bind too strongly to exclude the coordination of proteins [15]. Since both vanadium(V) and ...
... vanadium(IV)(VO2+) and vanadium(V)([VO4]3-) ions. We used vanadium(IV) ions coordinated by iminodiacetic acid (IDA), because free vanadium(IV) ions precipitate at neutral pH. IDA was chosen because it does not bind too strongly to exclude the coordination of proteins [15]. Since both vanadium(V) and ...
PCNA Protein Expression during Spermatogenesis of the
... volume of 0.25 M sucrose including 10 µg/ml leupeptin and 100 mM phenylmethanesulfonyl fluoride. After centrifugation at 9000 g for 10 min, the supernatant was incubated with 10 volumes of immunoprecipitation buffer (10 mM Tris-HCl, 0.1 M NaCl, 1 mM EDTA, 1% Nonidet P-40, pH 7.5) containing 50 µl of ...
... volume of 0.25 M sucrose including 10 µg/ml leupeptin and 100 mM phenylmethanesulfonyl fluoride. After centrifugation at 9000 g for 10 min, the supernatant was incubated with 10 volumes of immunoprecipitation buffer (10 mM Tris-HCl, 0.1 M NaCl, 1 mM EDTA, 1% Nonidet P-40, pH 7.5) containing 50 µl of ...
Chemistry 433 BIOCHEMISTRY LABORATORY MANUAL
... considering the content of each section. The expected content for each section of a scientific paper is discussed below. (Remember that you will probably not write the paper in this order.) Title Page: This should include the title of your report, the author’s name (i.e. your name), your lab partner ...
... considering the content of each section. The expected content for each section of a scientific paper is discussed below. (Remember that you will probably not write the paper in this order.) Title Page: This should include the title of your report, the author’s name (i.e. your name), your lab partner ...
Phylogenetic and Conservational Analyses of Dengue Non
... East Asia and China. DENV 1 and 3 were phylogenetically closer among the four serotypes. They further showed more than 80% conservation between each other. DENV 4 showed a higher deviation from other three serotypes. Strains from DENV3 showed high level of sequence conservation, while it was highly ...
... East Asia and China. DENV 1 and 3 were phylogenetically closer among the four serotypes. They further showed more than 80% conservation between each other. DENV 4 showed a higher deviation from other three serotypes. Strains from DENV3 showed high level of sequence conservation, while it was highly ...
ASMS 2004 de Novo
... higher background in MS/MS spectra and undergo preferential cleavages. Selective fragmentation reactions limit the amount of de Novo peptide sequence information that can be obtained in these experiments. In this presentation, we report our investigation to overcome this obstacle by modifying peptid ...
... higher background in MS/MS spectra and undergo preferential cleavages. Selective fragmentation reactions limit the amount of de Novo peptide sequence information that can be obtained in these experiments. In this presentation, we report our investigation to overcome this obstacle by modifying peptid ...
Report of attending the international CILIA 2016 in Amsterdam
... that Drosophila sensory cilia lacking MKS proteins exhibit striking defects during development but only subtle defects in adults. Interestingly, their results converge to our work in the conclusion that MKS proteins may play a role during tissue development, independantly of ciliogenesis. ...
... that Drosophila sensory cilia lacking MKS proteins exhibit striking defects during development but only subtle defects in adults. Interestingly, their results converge to our work in the conclusion that MKS proteins may play a role during tissue development, independantly of ciliogenesis. ...
Effects of macromolecular crowding on protein folding and
... an average molecular mass of ~70 kDa. In addition to these compounds, it was decided to use BSA and chicken albumin (ovalbumin) as crowding agents, in order to monitor any effects due to more specific interactions of these proteins with refolding lysozyme. The results are summarized in Figure 2A. It ...
... an average molecular mass of ~70 kDa. In addition to these compounds, it was decided to use BSA and chicken albumin (ovalbumin) as crowding agents, in order to monitor any effects due to more specific interactions of these proteins with refolding lysozyme. The results are summarized in Figure 2A. It ...
FtsZ - Cytoskeleton, Inc.
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
Metabolism of sucrose and its five isomers by
... filled with anaerobic gas and, at intervals, 1 ml samples were withdrawn by insertion of a gas-flushed tuberculin syringe through the butyl rubber cap. The cells were removed by filtration through Millex-GS filter units (0n22 µm pore size ; Millipore) and filtrates were collected. Samples were heate ...
... filled with anaerobic gas and, at intervals, 1 ml samples were withdrawn by insertion of a gas-flushed tuberculin syringe through the butyl rubber cap. The cells were removed by filtration through Millex-GS filter units (0n22 µm pore size ; Millipore) and filtrates were collected. Samples were heate ...
The use of deuteration for the structural study of larger proteins
... • Not enough restraints to calculate accurate global folds (RMSD > 8Å). Particularly poor if large content of α-helices. ...
... • Not enough restraints to calculate accurate global folds (RMSD > 8Å). Particularly poor if large content of α-helices. ...
THE PLANT LECTINS
... “THE PLANT LECTINS” Definition of a Lectin “A protein (other than an anti-carbohydrate antibody) that specifically recognizes and binds to glycans without catalyzing a modification of the glycan.” The first lectins identified were derived from plants, specifically leguminous seeds. Until recently, ...
... “THE PLANT LECTINS” Definition of a Lectin “A protein (other than an anti-carbohydrate antibody) that specifically recognizes and binds to glycans without catalyzing a modification of the glycan.” The first lectins identified were derived from plants, specifically leguminous seeds. Until recently, ...
Formate Dehydrogenase, an Enzyme of Anaerobic Metabolism, Is
... Acid Sequence Analysis Chemical or enzymatic digestion was used to determine the internal sequence of the proteins. Chemical digestion with CNBr was according to the method of Gross (1967) with the following modifications. Isolated protein spots from 50 2D PAGE gels were pooled by electroblotting on ...
... Acid Sequence Analysis Chemical or enzymatic digestion was used to determine the internal sequence of the proteins. Chemical digestion with CNBr was according to the method of Gross (1967) with the following modifications. Isolated protein spots from 50 2D PAGE gels were pooled by electroblotting on ...
Purification and Characterization of Two Thermostable Proteases
... out at 4oC. The following buffers were used: buffer A, 50 mM Tris-HCl (pH 8.0); buffer B, buffer A containing 50% saturated ammonium sulfate. (1) Fractional ammonium sulfate precipitation: solid ammonium sulfate was added to the culture filtrate to 90% saturation. After 12 h, the resulting precipita ...
... out at 4oC. The following buffers were used: buffer A, 50 mM Tris-HCl (pH 8.0); buffer B, buffer A containing 50% saturated ammonium sulfate. (1) Fractional ammonium sulfate precipitation: solid ammonium sulfate was added to the culture filtrate to 90% saturation. After 12 h, the resulting precipita ...
Kinetic Role of Helix Caps in Protein Folding Is Context
... tetrameric native state (28). A range of kinetic effects has also been seen in single amino acid mutations at both helix ends in several other proteins (3, 5, 6). The monomeric λ repressor protein (λ6-85) is one of the fastest folding proteins known (29). It is small and helical, with short loops, h ...
... tetrameric native state (28). A range of kinetic effects has also been seen in single amino acid mutations at both helix ends in several other proteins (3, 5, 6). The monomeric λ repressor protein (λ6-85) is one of the fastest folding proteins known (29). It is small and helical, with short loops, h ...
A fluorophore ligase for site-specific protein labeling inside living cells
... gical pH, making it membrane-impermeant. We protected either the carboxylic acid alone or both the acid and the 7-hydroxyl group with acetoxymethyl (AM) groups (coumarin-AM2 ; structure shown in Fig. 3A), which are known to be cleaved inside mammalian cells by endogenous esterases (21). Over time, e ...
... gical pH, making it membrane-impermeant. We protected either the carboxylic acid alone or both the acid and the 7-hydroxyl group with acetoxymethyl (AM) groups (coumarin-AM2 ; structure shown in Fig. 3A), which are known to be cleaved inside mammalian cells by endogenous esterases (21). Over time, e ...
Proteomics of
... it is of interest that glycosylation not only contributes to the structural diversity of the protein family, but also modulates the ligand-binding capabilities of the glycosylated spermadhesins, that is, it abolishes their zona pellucida-binding activity without impairing the heparin-binding ability ...
... it is of interest that glycosylation not only contributes to the structural diversity of the protein family, but also modulates the ligand-binding capabilities of the glycosylated spermadhesins, that is, it abolishes their zona pellucida-binding activity without impairing the heparin-binding ability ...
The ATP-Cone: An Evolutionarily Mobile, ATP
... Allosteric regulation is a common mechanism by which the activity of enzymes is modulated by the concentrations of their products, substrates and other small regulatory molecules. Structural studies have suggested that these functions frequently reside in compact globular domains that are distinct f ...
... Allosteric regulation is a common mechanism by which the activity of enzymes is modulated by the concentrations of their products, substrates and other small regulatory molecules. Structural studies have suggested that these functions frequently reside in compact globular domains that are distinct f ...
Novel Riboswitch Ligand Analogs as Selective Inhibitors of Guanine
... Pyrimidine-based molecules that could fit into the guanine riboswitch aptamer binding site were selected based on molecular modeling of crystal structures [26,27] (Figure 2A). Using this approach, we identified two pyrimidine compounds 2,5,6triaminopyrimidin-4-one (PC1) and 2,6-diaminopyrimidin-4-on ...
... Pyrimidine-based molecules that could fit into the guanine riboswitch aptamer binding site were selected based on molecular modeling of crystal structures [26,27] (Figure 2A). Using this approach, we identified two pyrimidine compounds 2,5,6triaminopyrimidin-4-one (PC1) and 2,6-diaminopyrimidin-4-on ...
Turnover-based in vitro selection and evolution of biocatalysts from
... the molecules are trapped after turnover. Covalently trapped molecules remain bound after washing with 6 M guanidinium chloride (GdmCl). Of six scFv fragments initially analyzed, two (TT1 and BB3) exhibited slow, progressive binding to the anchored DFMPP substrate upon prolonged exposure (Fig. 2), s ...
... the molecules are trapped after turnover. Covalently trapped molecules remain bound after washing with 6 M guanidinium chloride (GdmCl). Of six scFv fragments initially analyzed, two (TT1 and BB3) exhibited slow, progressive binding to the anchored DFMPP substrate upon prolonged exposure (Fig. 2), s ...
Modulation of the Oligomerization State of the Bovine F1
... phenylmethylsulfonyl fluoride. One tablet of a protease inhibitor mixture (Roche Molecular Biochemicals, GmbH, Germany) was added at this step. Protein purifications were carried out at 4 °C. Bacterial cells containing the various proteins were broken by being passed twice through a French pressure ...
... phenylmethylsulfonyl fluoride. One tablet of a protease inhibitor mixture (Roche Molecular Biochemicals, GmbH, Germany) was added at this step. Protein purifications were carried out at 4 °C. Bacterial cells containing the various proteins were broken by being passed twice through a French pressure ...
Gene Section HDAC3 (histone deacetylase 3) Atlas of Genetics and Cytogenetics
... specific promoters. Thus, HDAC3 is able to regulate osteoblast differentiation and bone formation via its association with the osteoblast master protein, Runx2, and the inhibition of the trans-activity of Runx2. Likewise, in hematopoietic stem cells, HDAC3, but not other class I HDACs, directly asso ...
... specific promoters. Thus, HDAC3 is able to regulate osteoblast differentiation and bone formation via its association with the osteoblast master protein, Runx2, and the inhibition of the trans-activity of Runx2. Likewise, in hematopoietic stem cells, HDAC3, but not other class I HDACs, directly asso ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.