IndelFR: a database of indels in protein structures and their flanking
... evolutionary history (9,10). However, there are few studies of the sequence–structure–function relationships of indels. Indels usually occur as reverse turns or coils within loops in a domain, most often on the surface of proteins (11–13). Compared to deletions, a succession of insertions and rapid ...
... evolutionary history (9,10). However, there are few studies of the sequence–structure–function relationships of indels. Indels usually occur as reverse turns or coils within loops in a domain, most often on the surface of proteins (11–13). Compared to deletions, a succession of insertions and rapid ...
Regulation of the heat stress response in Arabidopsis by
... to heat stress (Fig. 1). Detached leaves were incubated in pre-heated (37 ◦ C) liquid medium and then placed at 37 ◦ C for 1 h. As shown by in-gel kinase assays, several MBP-phosphorylating protein kinases in the range between 40–72 kDa become activated by heat stress (Fig. 1A). The strongest activi ...
... to heat stress (Fig. 1). Detached leaves were incubated in pre-heated (37 ◦ C) liquid medium and then placed at 37 ◦ C for 1 h. As shown by in-gel kinase assays, several MBP-phosphorylating protein kinases in the range between 40–72 kDa become activated by heat stress (Fig. 1A). The strongest activi ...
AlgaVia® Whole Algae Ingredients
... This vegan protein option contains all essential amino acids and has a high digestibility of 88%. Natural encapsulation allows increased protein fortification, whilst remaining stable in a low pH environment and no impact on viscosity. This makes it suitable for a wide range of applications includin ...
... This vegan protein option contains all essential amino acids and has a high digestibility of 88%. Natural encapsulation allows increased protein fortification, whilst remaining stable in a low pH environment and no impact on viscosity. This makes it suitable for a wide range of applications includin ...
Investigating semantic similarity measures across the
... It is also unclear how we should address the different link types. Except where stated explicitly (see Section 4.3), we consider the links equally. We took this approach because in GO there is a bias in link type usage between the different sub-ontologies (molecular function, 6207 is-a’s to 35 parto ...
... It is also unclear how we should address the different link types. Except where stated explicitly (see Section 4.3), we consider the links equally. We took this approach because in GO there is a bias in link type usage between the different sub-ontologies (molecular function, 6207 is-a’s to 35 parto ...
Angiotensin I-converting enzyme-inhibitory peptide fractions
... 1.4 29 cm, id Sephadex G-200 column (Pharmacia, Uppsala, Sweden). The column was previously equilibrated with buffer A. Onemillilitre fractions, 0.4 ml/min flow rate, were collected at room temperature and read at 280 nm. A low range molecular weight marker (Sigma–Aldrich, St. Louis, Mo., USA) was ...
... 1.4 29 cm, id Sephadex G-200 column (Pharmacia, Uppsala, Sweden). The column was previously equilibrated with buffer A. Onemillilitre fractions, 0.4 ml/min flow rate, were collected at room temperature and read at 280 nm. A low range molecular weight marker (Sigma–Aldrich, St. Louis, Mo., USA) was ...
Homology Modeling a Fast Tool for Drug Discovery
... the tough requirement of force field and enormous conformation searching, because it deals with the calculation of a force field and replaces it in large part, with the counting of sequence identities [7] . The method is based on the fact that structural conformation of a protein is more highly cons ...
... the tough requirement of force field and enormous conformation searching, because it deals with the calculation of a force field and replaces it in large part, with the counting of sequence identities [7] . The method is based on the fact that structural conformation of a protein is more highly cons ...
Effects of rare codon clusters on high-level expression
... cumulative effects o f severe physiological stress. In the latter case, the objectives are to isolate enough heterologous protein either to do some structural and biological studies or to meet some commercial targets. These systems generally use fermentation vessels, with induction o f the desired p ...
... cumulative effects o f severe physiological stress. In the latter case, the objectives are to isolate enough heterologous protein either to do some structural and biological studies or to meet some commercial targets. These systems generally use fermentation vessels, with induction o f the desired p ...
3. Orm proteins
... • Provides a starting point for studying how protein and lipid synthesis is coordinated during membrane biogenesis. • Disregulation of sphingolipid metabolism may contribute to the development of childhood asthma. • New Approach, which allow studies in proteins with know function. ...
... • Provides a starting point for studying how protein and lipid synthesis is coordinated during membrane biogenesis. • Disregulation of sphingolipid metabolism may contribute to the development of childhood asthma. • New Approach, which allow studies in proteins with know function. ...
New cell-based HTRF® assays for the exploration of Wnt signaling
... range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation. Hence its inactivation has pleiotropic pathophysiological effects (ref 1). Drug discovery and experiments - Extensive research in stabilizing or destabilizing β-catenin has enabled the discovery ...
... range of cellular processes, ranging from glycogen metabolism to cell cycle regulation and proliferation. Hence its inactivation has pleiotropic pathophysiological effects (ref 1). Drug discovery and experiments - Extensive research in stabilizing or destabilizing β-catenin has enabled the discovery ...
The Anaerobic (Class III) Ribonucleotide Reductase from Lactococcus lactis
... Expression and Purification of NrdD—E. coli IG016 was grown microaerophilically in 1.6-liter batches at 37 °C in LB medium containing 2% glucose, 150 g/ml kanamycin, and 34 g/ml chloramphenicol with continuous flow-through of 4% CO2 and 96% N2. When the culture had reached mid-log phase (A550 ⫽ 0. ...
... Expression and Purification of NrdD—E. coli IG016 was grown microaerophilically in 1.6-liter batches at 37 °C in LB medium containing 2% glucose, 150 g/ml kanamycin, and 34 g/ml chloramphenicol with continuous flow-through of 4% CO2 and 96% N2. When the culture had reached mid-log phase (A550 ⫽ 0. ...
Preparing and Analyzing Expressed Sequence Tags (ESTs) Library
... intake of newborn lambs in the first days of their lives plays essential role in their healthy growth and reaching to ideal market weight [9]. The raisers are desired to breed very productive animal, so colostrums secretion is a crucially important stage for breeding. Because the compounds of colost ...
... intake of newborn lambs in the first days of their lives plays essential role in their healthy growth and reaching to ideal market weight [9]. The raisers are desired to breed very productive animal, so colostrums secretion is a crucially important stage for breeding. Because the compounds of colost ...
Responses of cows to abomasal infusion of lysine and methionine at
... Effective ruminal degradabilities o f the different feed fractions were calculated according to 0 r s k o v and McDonald (1979) using a fractional passage rate o f 6.5%/h. Plasma urea N concentration was determined according to Coulomb and Faverau (1963) on pooled 8 h samples. Whole milk samples (0. ...
... Effective ruminal degradabilities o f the different feed fractions were calculated according to 0 r s k o v and McDonald (1979) using a fractional passage rate o f 6.5%/h. Plasma urea N concentration was determined according to Coulomb and Faverau (1963) on pooled 8 h samples. Whole milk samples (0. ...
OUR PRODUCTS
... America. It has been cultivated in South American Andes since at least 3,000 B.C. and has been a staple food of millions of native inhabitants. The ancient Incas called quinoa the "mother grain" and revered it as sacred. Each year at planting time it was traditional for the Inca leader to plant the ...
... America. It has been cultivated in South American Andes since at least 3,000 B.C. and has been a staple food of millions of native inhabitants. The ancient Incas called quinoa the "mother grain" and revered it as sacred. Each year at planting time it was traditional for the Inca leader to plant the ...
View PDF - Faculty Web Sites at the University of Virginia
... Mutations of nod, another Drosophila protein, yield phenotypes that are nearly identical to those of ncd mutants, but are restricted to non-exchange chromosomes [7*]. Although mutants for either nod or ncd are recessive, double heterozygotes have a phenotype similar to that of homozygous nod mutants ...
... Mutations of nod, another Drosophila protein, yield phenotypes that are nearly identical to those of ncd mutants, but are restricted to non-exchange chromosomes [7*]. Although mutants for either nod or ncd are recessive, double heterozygotes have a phenotype similar to that of homozygous nod mutants ...
Crystallization and X Ray Diffraction Data Analyses of the Enzyme
... electrophile coenzyme, essential for its correct folding and catalysis.21-23 Currently, there are only three UH structures determined crystallographically [Protein Data Bank, PDB, codes 1uwk, 2fkn and 1x8724], however, it is noteworthy that the highest identity [to the one from Pseudomonas putida21] ...
... electrophile coenzyme, essential for its correct folding and catalysis.21-23 Currently, there are only three UH structures determined crystallographically [Protein Data Bank, PDB, codes 1uwk, 2fkn and 1x8724], however, it is noteworthy that the highest identity [to the one from Pseudomonas putida21] ...
Evolution of Function in Protein Superfamilies, from a Structural
... a source of structural domains and pairwise alignments. Conservation of these functional descriptions decreases in this order, and all of them are less conserved than protein structure. Zhang et al. (1999) found that for about 10 % of Escherichia coli proteins with signi®cant sequence identity to a ...
... a source of structural domains and pairwise alignments. Conservation of these functional descriptions decreases in this order, and all of them are less conserved than protein structure. Zhang et al. (1999) found that for about 10 % of Escherichia coli proteins with signi®cant sequence identity to a ...
Protein structural class prediction using predicted secondary
... which determines its function. Structure prediction is an important area of research since it helps one to understand or discover the function of unknown proteins. Details of the 3D structures of proteins are very complicated, irregular, and expensive to determine. Researchers try to find out overa ...
... which determines its function. Structure prediction is an important area of research since it helps one to understand or discover the function of unknown proteins. Details of the 3D structures of proteins are very complicated, irregular, and expensive to determine. Researchers try to find out overa ...
Overview of Urea and Creatinine
... rate (GFR.) Creatinine is produced endogenously within the body and is freely filtered by the glomerulus. These characteristics make creatinine a useful endogenous marker for creatinine clearance. If the GFR is decreased, as is in renal disease, creatinine clearance via the renal system is compromis ...
... rate (GFR.) Creatinine is produced endogenously within the body and is freely filtered by the glomerulus. These characteristics make creatinine a useful endogenous marker for creatinine clearance. If the GFR is decreased, as is in renal disease, creatinine clearance via the renal system is compromis ...
Gene Section BOK (Bcl2-related ovarian killer) Atlas of Genetics and Cytogenetics
... Alternative splicing results in expression of two mRNA variants. The full-length (Bok-L) mRNA comprises 2.6 kb with the 639 bp open reading frame. The truncated form (Bok-S) results from skipping of exon three and a deletion of 43 bp in the Bok-L coding region. It has been shown that transcription a ...
... Alternative splicing results in expression of two mRNA variants. The full-length (Bok-L) mRNA comprises 2.6 kb with the 639 bp open reading frame. The truncated form (Bok-S) results from skipping of exon three and a deletion of 43 bp in the Bok-L coding region. It has been shown that transcription a ...
Green Fluorescent Protein
... upwards, peaked at 250 µM, and then again decreased. The fluorescence was also highest after 12 h. The following formula (based on the 12 h measurements) was derived using multiple regressions, and it can be used in order to estimate the Pb2+ concentration in an unknown water sample with 95% accurac ...
... upwards, peaked at 250 µM, and then again decreased. The fluorescence was also highest after 12 h. The following formula (based on the 12 h measurements) was derived using multiple regressions, and it can be used in order to estimate the Pb2+ concentration in an unknown water sample with 95% accurac ...
sequence-structure relationship - HAL
... Knowledge of protein three-dimensional (3D) structures of contributes to understand their biological functions (Baker and Sali, 2001). Predicting protein structures from amino acid sequences constitutes a major scientific challenge when both X-ray crystallography and nuclear magnetic resonance analy ...
... Knowledge of protein three-dimensional (3D) structures of contributes to understand their biological functions (Baker and Sali, 2001). Predicting protein structures from amino acid sequences constitutes a major scientific challenge when both X-ray crystallography and nuclear magnetic resonance analy ...
Blamires SJ, Wu CL, Tso IM (2012)
... greater in orb web spiders that produce MaSp2 predominant silks. Alternatively, (2) silk expression responses to protein intake may be explained by phylogeny [44,45]. In this case, we would expect that the glutamine and proline compositions of A. aetherea and C. moluccensis to exhibit similar shifts ...
... greater in orb web spiders that produce MaSp2 predominant silks. Alternatively, (2) silk expression responses to protein intake may be explained by phylogeny [44,45]. In this case, we would expect that the glutamine and proline compositions of A. aetherea and C. moluccensis to exhibit similar shifts ...
In Silico Prediction of the Peroxisomal Proteome in Fungi, Plants
... decision tree to sort proteins among several different compartments. In PSORT, the PTS1 motif [AS][HKR]-L is used as a marker for peroxisomal location along with amino acid composition over the entire protein. The performance on peroxisomal proteins is modest, in the sense that many peroxisomal prot ...
... decision tree to sort proteins among several different compartments. In PSORT, the PTS1 motif [AS][HKR]-L is used as a marker for peroxisomal location along with amino acid composition over the entire protein. The performance on peroxisomal proteins is modest, in the sense that many peroxisomal prot ...
Genetic and biochemical identification of the
... after washing with buffer. The BL21(DE3)/pET21-ncgl2098 cellular lysate was then loaded. DS2098 had no His-tag, so it was not able to bind with resin and would be easily washed out with buffer. However, if CM0819 interacted with DS2098 and the two formed a complex, this DS2098 would remain within th ...
... after washing with buffer. The BL21(DE3)/pET21-ncgl2098 cellular lysate was then loaded. DS2098 had no His-tag, so it was not able to bind with resin and would be easily washed out with buffer. However, if CM0819 interacted with DS2098 and the two formed a complex, this DS2098 would remain within th ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.