High-Molecular-Weight Forms of Tyrosinase and the
... When the dopachrome tauto merase acti vity ofTRP-2 was purified as described previousl y [17 ,18] from detergent extracts of C loudman melanoma cells, tyrosin ase activity copurified wi th it through multiple steps (Table I). We have shown previo usly th at upon gelfiltration HPLC, dopachrome tautom ...
... When the dopachrome tauto merase acti vity ofTRP-2 was purified as described previousl y [17 ,18] from detergent extracts of C loudman melanoma cells, tyrosin ase activity copurified wi th it through multiple steps (Table I). We have shown previo usly th at upon gelfiltration HPLC, dopachrome tautom ...
Protein Structure Prediction Based on Neural Networks
... and knowledge of a protein’s 3D structure improves the analysis of protein functions, particularly for drug design [2]. Being aware of the 3D structure of a protein enables pharmacologists to select a binding protein to moderate its functions. Pharmacologists may choose a drug which is easy to synt ...
... and knowledge of a protein’s 3D structure improves the analysis of protein functions, particularly for drug design [2]. Being aware of the 3D structure of a protein enables pharmacologists to select a binding protein to moderate its functions. Pharmacologists may choose a drug which is easy to synt ...
Multiple Sequence Motifs in the Rubisco Small
... targeting, we constructed mutants, E1aT1[TTT/ AAA], E1aT1[FPL/AAA], and E1aT1[K/A], that contained Ala substitution of the three Thrs (TTT), the three hydrophobic amino acids (FPL), and the single Lys (K), respectively (Fig. 2C). These mutants were fused to GFP and then we examined their ability to ...
... targeting, we constructed mutants, E1aT1[TTT/ AAA], E1aT1[FPL/AAA], and E1aT1[K/A], that contained Ala substitution of the three Thrs (TTT), the three hydrophobic amino acids (FPL), and the single Lys (K), respectively (Fig. 2C). These mutants were fused to GFP and then we examined their ability to ...
SUPPLEMENTARY INFORMATION
... Supplementary Fig. 5. Effects of various salts on the thermostability of purified vcINDY ...
... Supplementary Fig. 5. Effects of various salts on the thermostability of purified vcINDY ...
introduction figures
... T7 promoter. The PE24 gene in this plasmid contains an Omp A signal sequence that is cleaved upon secretion of PE24 into the periplasm. The plasmid also contains repeat of the trinucleotide, CAT, that codes for a poly His sequence at the C-terminus of the PE24 protein. The incorporation of the desir ...
... T7 promoter. The PE24 gene in this plasmid contains an Omp A signal sequence that is cleaved upon secretion of PE24 into the periplasm. The plasmid also contains repeat of the trinucleotide, CAT, that codes for a poly His sequence at the C-terminus of the PE24 protein. The incorporation of the desir ...
Functional and structural roles of parasite-specific inserts in the bifunctional S-adenosylmethionine decarboxylase/ornithine
... 4.2.5.2) Dot-blot Westem analyses of SE-FPLC fractions. The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hou ...
... 4.2.5.2) Dot-blot Westem analyses of SE-FPLC fractions. The collected size-exclusion chromatography fractions were transferred to nitrocellulose membranes using a BioDot apparatus (Bio-Rad) and analysed by dot blot Western. The membranes were blocked in 3% w/v low fat milk powder in lxPBS for 16 hou ...
A1071 GM Canola MON88302 AppR SD1
... is lowest in the pollen (approximately 9 µg/g dry weight) and highest in leaves at the stage where stem elongation (bolting) begins (approximately 230 µg/g dry weight). The level in the mature seed is approximately 27 µg/g dry weight. The identity of MON88302-derived CP4 EPSPS was confirmed by a num ...
... is lowest in the pollen (approximately 9 µg/g dry weight) and highest in leaves at the stage where stem elongation (bolting) begins (approximately 230 µg/g dry weight). The level in the mature seed is approximately 27 µg/g dry weight. The identity of MON88302-derived CP4 EPSPS was confirmed by a num ...
Greenmarket`s Grains Guide
... are not a living seed. Currently at Greenmarket, whole “live oats” and “Scottish oats” (rough chopped) are available. These oats have not been steamed to preserve them so while they will have a limited shelf life, they contain the full nutritional value and flavor of the whole oat. Once the bran is c ...
... are not a living seed. Currently at Greenmarket, whole “live oats” and “Scottish oats” (rough chopped) are available. These oats have not been steamed to preserve them so while they will have a limited shelf life, they contain the full nutritional value and flavor of the whole oat. Once the bran is c ...
Strain in Protein Structures as Viewed Through Nonrotameric Side
... of the rotameric states. Schrauber et al.1 used a much larger protein structure set and observed that a significant fraction (up to 30%) of particular side-chain types could not be assigned to a rotameric state based on the criterion that rotameric side chains can deviate in each of the 1 and 2 an ...
... of the rotameric states. Schrauber et al.1 used a much larger protein structure set and observed that a significant fraction (up to 30%) of particular side-chain types could not be assigned to a rotameric state based on the criterion that rotameric side chains can deviate in each of the 1 and 2 an ...
148 - Blue Ridge Institute for Medical Research
... Protein kinases play a predominant regulatory role in nearly every aspect of cell biology and they can modify the function of a protein in almost every conceivable way. Protein phosphorylation can increase or decrease enzyme activity and it can alter other biological activities such as transcription ...
... Protein kinases play a predominant regulatory role in nearly every aspect of cell biology and they can modify the function of a protein in almost every conceivable way. Protein phosphorylation can increase or decrease enzyme activity and it can alter other biological activities such as transcription ...
Protein Interaction Technical Handbook
... co-immunoprecipitation, pull-down or far-Western methods. Transient interactions are expected to control the majority of cellular processes. As the name implies, transient interactions are on/off or temporary in nature and typically require a specific set of conditions that promote the interaction. ...
... co-immunoprecipitation, pull-down or far-Western methods. Transient interactions are expected to control the majority of cellular processes. As the name implies, transient interactions are on/off or temporary in nature and typically require a specific set of conditions that promote the interaction. ...
Application of Silicon Nanowire in Biosensor
... transistors (FET), optoelectronics, solar cell and especially biosensor • Using SiNW to detect protein, DNA and single molecules. ...
... transistors (FET), optoelectronics, solar cell and especially biosensor • Using SiNW to detect protein, DNA and single molecules. ...
Purified Hexameric Epstein-Barr Virus-Encoded BARF1 Protein for Measuring Anti-BARF1 Antibody
... twice with phosphate-buffered saline (PBS), and serum-free medium was added for 24 h, after which the medium was harvested. Medium (300 ml) was diluted 1:1 in binding buffer (20 mM Tris, pH 7.4, 0.5 M NaCl) and incubated for 1 h with 10 ml concanavalin A bead slurry (Sigma-Aldrich). After being wash ...
... twice with phosphate-buffered saline (PBS), and serum-free medium was added for 24 h, after which the medium was harvested. Medium (300 ml) was diluted 1:1 in binding buffer (20 mM Tris, pH 7.4, 0.5 M NaCl) and incubated for 1 h with 10 ml concanavalin A bead slurry (Sigma-Aldrich). After being wash ...
Sequence and Tissue Distribution of a Second Protein of Hepatic
... Rabbits were immunized with Img of peptide in complete Freund's adjuvant (mixed 2:1 with aqueous peptide solution) intradermally and intramuscularly. The animals were boosted 2 mo later with 0.5 nag peptide in complete Freund's adjuvant and bled 1 wk after boost. For purposes of affinity purificatio ...
... Rabbits were immunized with Img of peptide in complete Freund's adjuvant (mixed 2:1 with aqueous peptide solution) intradermally and intramuscularly. The animals were boosted 2 mo later with 0.5 nag peptide in complete Freund's adjuvant and bled 1 wk after boost. For purposes of affinity purificatio ...
Ribosomes of Mouse Liver following
... while membranous material is trapped at the interface of the sucrose layers. It has been shown (31) and confirmed in this work (Table 4) that following the 1st centrifugation for sedimentation of free ribosomes some free monomers remain in the overlying 2.0 M sucrose cushion. Therefore, this cushion ...
... while membranous material is trapped at the interface of the sucrose layers. It has been shown (31) and confirmed in this work (Table 4) that following the 1st centrifugation for sedimentation of free ribosomes some free monomers remain in the overlying 2.0 M sucrose cushion. Therefore, this cushion ...
Molecular characterization of carnitine palmitoyltransferase 1C Esther Gratacòs i Batlle
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
... WARNING. On having consulted this thesis you’re accepting the following use conditions: Spreading this thesis by the TDX (www.tesisenxarxa.net) service has been authorized by the titular of the intellectual property rights only for private uses placed in investigation and teaching activities. Reprod ...
EVOLUTIONARILY RELATED INSERTION PATHWAYS OF
... the inner membrane or thylakoid membrane, respectively, often contain bipartite presequences. The first part of these presequences target the protein to the lumen of the organelle, whereas the second part mediates membrane integration. How might topogenic sequences operate? Over two decades ago Blob ...
... the inner membrane or thylakoid membrane, respectively, often contain bipartite presequences. The first part of these presequences target the protein to the lumen of the organelle, whereas the second part mediates membrane integration. How might topogenic sequences operate? Over two decades ago Blob ...
Protein relative abundance patterns associated with sucrose
... The lesions of enamel caries can be considered as the outcome of dysbiotic changes in the biofilm community of supragingival dental plaque [1, 2]. Demineralization occurs as the cumulative outcome of repeated shifts towards a less diverse microbiota that produces and tolerates a low pH environment i ...
... The lesions of enamel caries can be considered as the outcome of dysbiotic changes in the biofilm community of supragingival dental plaque [1, 2]. Demineralization occurs as the cumulative outcome of repeated shifts towards a less diverse microbiota that produces and tolerates a low pH environment i ...
Comparison of the activities of protein disulphide
... originally by Haber & Anfinsen (1962) and was based on a previous procedure (Hillson et al., 1984). RNAase was reduced by incubation at 25 mg/ml (1.8 mM) in 50 mM-Tris/HCl buffer, pH 8.6, containing 130 mMdithiothreitol and 10 M-urea. The flask was flushed gently with N29 sealed, and left in the dar ...
... originally by Haber & Anfinsen (1962) and was based on a previous procedure (Hillson et al., 1984). RNAase was reduced by incubation at 25 mg/ml (1.8 mM) in 50 mM-Tris/HCl buffer, pH 8.6, containing 130 mMdithiothreitol and 10 M-urea. The flask was flushed gently with N29 sealed, and left in the dar ...
Zaenab Aljassim thesis-6_1
... Tuberculosis is a bacterial infection that affects one-third of the global population. The pathogen responsible for the vast majority of these cases is Mycobacterium tuberculosis and the current vaccines are insufficiently effective. The current vaccine against Tuberculosis is the live bacille Calme ...
... Tuberculosis is a bacterial infection that affects one-third of the global population. The pathogen responsible for the vast majority of these cases is Mycobacterium tuberculosis and the current vaccines are insufficiently effective. The current vaccine against Tuberculosis is the live bacille Calme ...
draft safety assessment report - Food Standards Australia New
... FSANZ’s role is to protect the health and safety of people in Australia and New Zealand through the maintenance of a safe food supply. FSANZ is a partnership between ten Governments: the Commonwealth; Australian States and Territories; and New Zealand. It is a statutory authority under Commonwealth ...
... FSANZ’s role is to protect the health and safety of people in Australia and New Zealand through the maintenance of a safe food supply. FSANZ is a partnership between ten Governments: the Commonwealth; Australian States and Territories; and New Zealand. It is a statutory authority under Commonwealth ...
Utilisation of Whey
... IgA, IgG, IgE and IgM, with molecular weights of 150,000 to 450,000 or greater. Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory form of IgA which is produced in the mammary gland in response to infecti ...
... IgA, IgG, IgE and IgM, with molecular weights of 150,000 to 450,000 or greater. Some of them represent the normal antibody components of the cow and are the same as those found in the bloodstream; however, there is a secretory form of IgA which is produced in the mammary gland in response to infecti ...
Protein purification
Protein purification is a series of processes intended to isolate one or a few proteins from a complex mixture, usually cells, tissues or whole organisms. Protein purification is vital for the characterization of the function, structure and interactions of the protein of interest. The purification process may separate the protein and non-protein parts of the mixture, and finally separate the desired protein from all other proteins. Separation of one protein from all others is typically the most laborious aspect of protein purification. Separation steps usually exploit differences in protein size, physico-chemical properties, binding affinity and biological activity. The pure result may be termed protein isolate.The methods used in protein purification can roughly be divided into analytical and preparative methods. The distinction is not exact, but the deciding factor is the amount of protein that can practically be purified with that method. Analytical methods aim to detect and identify a protein in a mixture, whereas preparative methods aim to produce large quantities of the protein for other purposes, such as structural biology or industrial use. In general, the preparative methods can be used in analytical applications, but not the other way around.