Axonal of Kinesin in the Chain Isoforms
... maintenance of axonal arbors. There is little if any protein synthesis in the axon, so polypeptides needed in axons or terminals must be transported from the cell body to their site of utilization. Because axons in humans can span a meter or more and axons in larger animals may extend even further, ...
... maintenance of axonal arbors. There is little if any protein synthesis in the axon, so polypeptides needed in axons or terminals must be transported from the cell body to their site of utilization. Because axons in humans can span a meter or more and axons in larger animals may extend even further, ...
Copyright 2010 Scott R
... Na(H3BNMe2BH3), in tetrahydrofuran produces the new complex Th(H3BNMe2BH3)4. The thorium center forms bonds with fifteen hydrogen atoms; accordingly, this is the first example of a fifteen-coordinate atom of any kind. As determined by both single crystal X-ray and single crystal neutron diffraction ...
... Na(H3BNMe2BH3), in tetrahydrofuran produces the new complex Th(H3BNMe2BH3)4. The thorium center forms bonds with fifteen hydrogen atoms; accordingly, this is the first example of a fifteen-coordinate atom of any kind. As determined by both single crystal X-ray and single crystal neutron diffraction ...
{PDOC00000} {BEGIN
... [ 2] Pugh E.L., Wakil S.J. J. Biol. Chem. 240:4727-4733(1965). [ 3] Witkowski A., Rangan V.S., Randhawa Z.I., Amy C.M., Smith Eur. J. Biochem. 198:571-579(1991). [ 4] Bibb M.J., Biro S., Motamedi H., Collins J.F., Hutchinson EMBO J. 8:2727-2736(1989). [ 5] Sherman D.H., Malpartida F., Bibb M.J., Kie ...
... [ 2] Pugh E.L., Wakil S.J. J. Biol. Chem. 240:4727-4733(1965). [ 3] Witkowski A., Rangan V.S., Randhawa Z.I., Amy C.M., Smith Eur. J. Biochem. 198:571-579(1991). [ 4] Bibb M.J., Biro S., Motamedi H., Collins J.F., Hutchinson EMBO J. 8:2727-2736(1989). [ 5] Sherman D.H., Malpartida F., Bibb M.J., Kie ...
Lipoprotein structure
... cholesterol-to-phospholipid ratio in LDL (M.C. Phillips, 1989). The surface monolayer of VLDL is even more fluid than that of HDL due to differences in the monolayer lipids. Apolipoproteins exert little or no effect on the average dynamic properties of the surface lipids especially in LDL and VLDL. ...
... cholesterol-to-phospholipid ratio in LDL (M.C. Phillips, 1989). The surface monolayer of VLDL is even more fluid than that of HDL due to differences in the monolayer lipids. Apolipoproteins exert little or no effect on the average dynamic properties of the surface lipids especially in LDL and VLDL. ...
Structure-Function Analysis of SH3 Domains: SH3
... tyrosine-phosphorylated proteins from SRD 3T3 cells. Figure 3 shows an antiphosphotyrosine immunoblot of proteins affinity purified from SRD 3T3 cells by using glutathione-agarose bound with equal amounts of either GST (lane 1) or GST-SH3 fusion proteins (lanes 2 to 6). Consistent with previous find ...
... tyrosine-phosphorylated proteins from SRD 3T3 cells. Figure 3 shows an antiphosphotyrosine immunoblot of proteins affinity purified from SRD 3T3 cells by using glutathione-agarose bound with equal amounts of either GST (lane 1) or GST-SH3 fusion proteins (lanes 2 to 6). Consistent with previous find ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... Fig. 1. Analysis of complexes formed between Hsp70, BAG-1 and non-native substrate. (A) Detection of complexes between Hsp70, BAG-1 and RCMLA by gel sieving chromatography. Reaction mixtures containing 14 μM GST-BAG-1 ⫹ 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (s), 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (u), or 2 ...
... Fig. 1. Analysis of complexes formed between Hsp70, BAG-1 and non-native substrate. (A) Detection of complexes between Hsp70, BAG-1 and RCMLA by gel sieving chromatography. Reaction mixtures containing 14 μM GST-BAG-1 ⫹ 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (s), 14 μM Hsp70 ⫹ 2.8 μM [125I]RCMLA (u), or 2 ...
The cell biology of major histocompatibility complex class
... These studies suggest that ERp57 fulfils a structural role within the PLC; indeed in cellular lysates only the tapasin–ERp57 heterodimer, and not free tapasin, interacts with MHC I molecules and facilitates peptide loading (30). Interaction of ERp57 with both tapasin and calreticulin may strengthen ...
... These studies suggest that ERp57 fulfils a structural role within the PLC; indeed in cellular lysates only the tapasin–ERp57 heterodimer, and not free tapasin, interacts with MHC I molecules and facilitates peptide loading (30). Interaction of ERp57 with both tapasin and calreticulin may strengthen ...
Investigations of non-enzymatic glycation with new bioanalytical
... Desalting of proteins and peptides by solid phase extraction (SPE) Solid phase extraction (SPE) is an easily applicable method most frequently used for desalting, purification, preconcentration and fractionation of a complex mixture of peptides. Fractionation prior to analyses can have a significant ...
... Desalting of proteins and peptides by solid phase extraction (SPE) Solid phase extraction (SPE) is an easily applicable method most frequently used for desalting, purification, preconcentration and fractionation of a complex mixture of peptides. Fractionation prior to analyses can have a significant ...
New Generation Collagens
... resistant gel, makes it possible to emulsify with many other biologically active substances that are beneficial to our skin. Collagen is a word that is synonymous with many restorative and rejuvenating products. That is why in the beauty and pharmaceutical world it is frequently misused. The name co ...
... resistant gel, makes it possible to emulsify with many other biologically active substances that are beneficial to our skin. Collagen is a word that is synonymous with many restorative and rejuvenating products. That is why in the beauty and pharmaceutical world it is frequently misused. The name co ...
PDF File
... and its neighbor to either side (Freier et al., 1986; Mathews et al., 1999). In 1983, concomitant with publication of a possible secondary structure for the Tetrahymena group I intron from phylogenetic comparisons (Michel and Dujon, 1983; Waring et al., 1983), Cech et al. published a secondary struc ...
... and its neighbor to either side (Freier et al., 1986; Mathews et al., 1999). In 1983, concomitant with publication of a possible secondary structure for the Tetrahymena group I intron from phylogenetic comparisons (Michel and Dujon, 1983; Waring et al., 1983), Cech et al. published a secondary struc ...
Organization of Physical Interactomes as
... required the development of novel computational techniques (see Methods for more details). Second, in the first large-scale analysis of this type, we apply our procedure to the S. cerevisiae protein-protein interactome. In total, ...
... required the development of novel computational techniques (see Methods for more details). Second, in the first large-scale analysis of this type, we apply our procedure to the S. cerevisiae protein-protein interactome. In total, ...
FUNCTIONAL CONSEQUENCES OF THE DIRECT PHYSICAL CBP/p300
... Clues to the molecular mechanisms of leukemogenesis have been provided by the discovery of non-random chromosomal abnormalities in the leukemic cells. More than half of all leukemias have detectable chromosomal abnormalities by karyotypic analysis (2). Some of these abnormalities are consistently as ...
... Clues to the molecular mechanisms of leukemogenesis have been provided by the discovery of non-random chromosomal abnormalities in the leukemic cells. More than half of all leukemias have detectable chromosomal abnormalities by karyotypic analysis (2). Some of these abnormalities are consistently as ...
Forced Expression of Dystrophin Deletion Constructs Reveals
... muscle has not yet been fully elucidated, although it is thought to provide a crucial link between the intracellular actin cytoskeleton and the extracellular matrix (15). The mdx mouse contains a nonsense mutation in the dystrophin gene that leads to a complete absence of dystrophin in muscle, which ...
... muscle has not yet been fully elucidated, although it is thought to provide a crucial link between the intracellular actin cytoskeleton and the extracellular matrix (15). The mdx mouse contains a nonsense mutation in the dystrophin gene that leads to a complete absence of dystrophin in muscle, which ...
Phylogenetic Classification of Protozoa Based on the
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
Phylogenetic Classification of Protozoa Based on the Structure of
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
Lipopolysaccharide-bound structure of the antimicrobial peptide
... 180 rpm. Following incubation, samples were diluted, and 50-μL aliquots of samples were plated on TSB agar plates. Surviving bacterial rates were determined relative to the surviving colonies of untreated control cultures after 12–14 h of incubation at 30°C. The MBC was determined by the lowest conc ...
... 180 rpm. Following incubation, samples were diluted, and 50-μL aliquots of samples were plated on TSB agar plates. Surviving bacterial rates were determined relative to the surviving colonies of untreated control cultures after 12–14 h of incubation at 30°C. The MBC was determined by the lowest conc ...
The Plant Cell - Department of Biology
... KIC with Its Single EF-Hand Motif Binds Ca 2 Because KIC has a predicted EF-hand motif, we tested its Ca 2 binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion pro ...
... KIC with Its Single EF-Hand Motif Binds Ca 2 Because KIC has a predicted EF-hand motif, we tested its Ca 2 binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion pro ...
Schubert, C. M., R. Lin, C. J. de Vries, R. H. A.
... among other cell types. Mutations in any of several par genes, or in a gene called mex-1, result in misexpression of SKN-1 in anterior blastomeres; such mutants have abnormally large numbers of muscles (see Bowerman and Shelton, 1999). We performed a genetic screen for maternal effect lethal mutants ...
... among other cell types. Mutations in any of several par genes, or in a gene called mex-1, result in misexpression of SKN-1 in anterior blastomeres; such mutants have abnormally large numbers of muscles (see Bowerman and Shelton, 1999). We performed a genetic screen for maternal effect lethal mutants ...
Expression, purification and characterization of azurin derived
... therapeutic proteins, antibodies and small organic molecules. Most of these peptides are designed based on truncate fragments of therapeutic proteins and antibodies. Nowadays, these peptides are used in many pathologies like diabetes, microbial infections and oncology. (Lu et al., 2006; Vlieghe et a ...
... therapeutic proteins, antibodies and small organic molecules. Most of these peptides are designed based on truncate fragments of therapeutic proteins and antibodies. Nowadays, these peptides are used in many pathologies like diabetes, microbial infections and oncology. (Lu et al., 2006; Vlieghe et a ...
Formation of a highly peptide-receptive state of class II MHC.
... detergent-solubilized class II molecules from B cell lines. These studies found that antigenic peptides bind to purified class II molecules to form stable complexes, but that this binding is very slow (kon ≈ 1–100 M21 s21) (Buus et al., 1986; Roche and Cresswell, 1990; Roof et al., 1990). It was lat ...
... detergent-solubilized class II molecules from B cell lines. These studies found that antigenic peptides bind to purified class II molecules to form stable complexes, but that this binding is very slow (kon ≈ 1–100 M21 s21) (Buus et al., 1986; Roche and Cresswell, 1990; Roof et al., 1990). It was lat ...
The Platform Protein Is Essential for Type IV Pilus
... both the motor and alignment subcomplexes in the T4P and T2S systems have been suggested to transduce the conformational changes of the ATPases (26, 27), making it challenging to propose a unifying model of fiber assembly and function. In the P. aeruginosa T4aP system, the platform protein PilC was ...
... both the motor and alignment subcomplexes in the T4P and T2S systems have been suggested to transduce the conformational changes of the ATPases (26, 27), making it challenging to propose a unifying model of fiber assembly and function. In the P. aeruginosa T4aP system, the platform protein PilC was ...
Kinetic modelling of the Maillard reaction between proteins and sugars
... multiresponse modelling. Thebasic idea istotake intoaccount asmanyresponses aspossible at once, as opposed to only one response. By applying multiresponse modelling, more realistic models and more accurate parameter estimates (namely of rate constants and activation energies) will be obtained and th ...
... multiresponse modelling. Thebasic idea istotake intoaccount asmanyresponses aspossible at once, as opposed to only one response. By applying multiresponse modelling, more realistic models and more accurate parameter estimates (namely of rate constants and activation energies) will be obtained and th ...
Crosslinking Technical Handbook
... maleimides because they will compete for coupling sites. For example, if DTT were used to reduce disulfides in a protein to make sulfhydryl groups available for conjugation, the DTT would have to be thoroughly removed using a desalting column before initiating the maleimide reaction. Interestingly, ...
... maleimides because they will compete for coupling sites. For example, if DTT were used to reduce disulfides in a protein to make sulfhydryl groups available for conjugation, the DTT would have to be thoroughly removed using a desalting column before initiating the maleimide reaction. Interestingly, ...
pdf file - John Innes Centre
... GlnK was found in eluates from wild type or glnK⫹⌬amtB cells (Fig. 1B, lanes 2 and 3) indicating that GlnK itself has no ability to interact with the beads. This result was also confirmed using purified GlnK (data not shown). Co-purification of AmtB6H and GlnK from GT1000(pJT6) extracts was also dem ...
... GlnK was found in eluates from wild type or glnK⫹⌬amtB cells (Fig. 1B, lanes 2 and 3) indicating that GlnK itself has no ability to interact with the beads. This result was also confirmed using purified GlnK (data not shown). Co-purification of AmtB6H and GlnK from GT1000(pJT6) extracts was also dem ...
Cyclol
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.