Structure and properties of the outer membranes of Brucella abortus
... (362 vs. 308 amino acids), this higher hydrophobicity would be in keeping with the lipid composition and overall OM properties (see Hydrophobicity). Group 3 Two proteins of 25 and 31 kDa with only 34% of identity are included in group 3 [50] and they are coded for by the omp25 and omp31 genes [5]. B ...
... (362 vs. 308 amino acids), this higher hydrophobicity would be in keeping with the lipid composition and overall OM properties (see Hydrophobicity). Group 3 Two proteins of 25 and 31 kDa with only 34% of identity are included in group 3 [50] and they are coded for by the omp25 and omp31 genes [5]. B ...
Universitat Autònoma de Barcelona
... Castillo V, Espargaró A, Gordo V, Vendrell J, Ventura S Proteomics, Dec 2010;10(23:4172-4185 5. Studies on bacterial inclusion bodies De Groot NS, Espargaró A, Morell M, Ventura S Future Microbiology, Aug 2008;3(4):423-435 6. Inclusion bodies: specificity in their aggregation process and amyloid-lik ...
... Castillo V, Espargaró A, Gordo V, Vendrell J, Ventura S Proteomics, Dec 2010;10(23:4172-4185 5. Studies on bacterial inclusion bodies De Groot NS, Espargaró A, Morell M, Ventura S Future Microbiology, Aug 2008;3(4):423-435 6. Inclusion bodies: specificity in their aggregation process and amyloid-lik ...
Teaching with SCIGRESS - Photochemical Dynamics Group
... Complete Pre-lab Assignment: Molecular Modeling before beginning the experiment. ...
... Complete Pre-lab Assignment: Molecular Modeling before beginning the experiment. ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
... quence (E steban, 1993). E steban (1993) has recently also identified a 23 k D a polypeptide as a C -term inal fragm ent o f Lcm startin g at am ino acid position 923. The simi lar size and sequence overlaps show th a t o u r poly peptides are closely related to th e latter. Since they are N -term ...
... quence (E steban, 1993). E steban (1993) has recently also identified a 23 k D a polypeptide as a C -term inal fragm ent o f Lcm startin g at am ino acid position 923. The simi lar size and sequence overlaps show th a t o u r poly peptides are closely related to th e latter. Since they are N -term ...
Functional Characterisation of Plant
... outer membrane proteome between plants and other groups of organisms. Whilst the mitochondrial outer membrane proteome has been studied in model organisms such as Saccharomyces cerevisiae or Neurospora crassa, the components present in Arabidopsis thaliana (Arabidopsis) have been mostly deduced from ...
... outer membrane proteome between plants and other groups of organisms. Whilst the mitochondrial outer membrane proteome has been studied in model organisms such as Saccharomyces cerevisiae or Neurospora crassa, the components present in Arabidopsis thaliana (Arabidopsis) have been mostly deduced from ...
The DELLA protein family and gibberellin signal transduction
... system. However, the DELLA proteins are also post-translationally modified which affects their activity. It is believed the DELLAs are modified both with O-linked N-Acetyl glucosamine for stability and phosphate groups to mark them for F-box recognition. However, the precise nature, and role of thes ...
... system. However, the DELLA proteins are also post-translationally modified which affects their activity. It is believed the DELLAs are modified both with O-linked N-Acetyl glucosamine for stability and phosphate groups to mark them for F-box recognition. However, the precise nature, and role of thes ...
Rubisco Synthesis, Assembly, Mechanism, and Regulation
... the enzyme requires an equivalent number of S-subunits to be fully active. Although the quaternary structures of the two forms are apparently very distinct, they are nevertheless related by the domain structure of the larger subunit and the nature of the subunit interactions. The first structural mo ...
... the enzyme requires an equivalent number of S-subunits to be fully active. Although the quaternary structures of the two forms are apparently very distinct, they are nevertheless related by the domain structure of the larger subunit and the nature of the subunit interactions. The first structural mo ...
... Polar transport–dependent local accumulation of auxin provides positional cues for multiple plant patterning processes. This directional auxin flow depends on the polar subcellular localization of the PIN auxin efflux regulators. Overexpression of the PINOID protein kinase induces a basal-toapical s ...
A B23-interacting sequence as a tool to visualize protein interactions
... Fig. 2. NoLS interacts directly with B23; this interaction involves the acidic stretches of B23. (A) GST and GST-NoLS pull-down assays performed using whole HeLa cell extracts. A sample (1%) of the extract used for each pull-down assay (Input), 1% of the supernatant (S) and 1.5% of the proteins elut ...
... Fig. 2. NoLS interacts directly with B23; this interaction involves the acidic stretches of B23. (A) GST and GST-NoLS pull-down assays performed using whole HeLa cell extracts. A sample (1%) of the extract used for each pull-down assay (Input), 1% of the supernatant (S) and 1.5% of the proteins elut ...
PDF
... directed against the human SmB/B⬘ (SNRPB – Human Genome Nomenclature Database) proteins. Only one protein band of the expected molecular mass was detected on a western blot using this antibody, demonstrating its specificity for SmB (Fig. 2C). Immunostained egg chambers showed an accumulation of endo ...
... directed against the human SmB/B⬘ (SNRPB – Human Genome Nomenclature Database) proteins. Only one protein band of the expected molecular mass was detected on a western blot using this antibody, demonstrating its specificity for SmB (Fig. 2C). Immunostained egg chambers showed an accumulation of endo ...
Designing logical codon reassignment
... This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. ...
... This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. ...
S C T
... resulting in their random distribution (Singer and Nicolson, 1972). However, to account for lateral heterogeneities in membranes, which is a result of proteinprotein, protein–lipid and lipid-lipid interactions, this model has recently been refined (Vereb et al., 2003). Membrane lipids are divided in ...
... resulting in their random distribution (Singer and Nicolson, 1972). However, to account for lateral heterogeneities in membranes, which is a result of proteinprotein, protein–lipid and lipid-lipid interactions, this model has recently been refined (Vereb et al., 2003). Membrane lipids are divided in ...
NF-kappaB : mini revisão The Rel/NF
... to clarify which Rel/NF-kB target genes are activated in a given response. As described above, the structures of several Rel/NF-kB dimers on DNA or bound to IkB are known. In all cases, these structures have been derived from molecules that contain almost exclusively residues from the RH domain. As ...
... to clarify which Rel/NF-kB target genes are activated in a given response. As described above, the structures of several Rel/NF-kB dimers on DNA or bound to IkB are known. In all cases, these structures have been derived from molecules that contain almost exclusively residues from the RH domain. As ...
Meiotic and Mitotic Recombination in Meiosis
... Another shortcoming of the two-pathway paradigm is that the proteins used to define these pathways have very different functions: Mus81–Mms4 is a Holliday junction resolvase whose activity presumably directly produces crossover products (Boddy et al. 2001) (i.e., it is a procrossover resolvase). Msh4 ...
... Another shortcoming of the two-pathway paradigm is that the proteins used to define these pathways have very different functions: Mus81–Mms4 is a Holliday junction resolvase whose activity presumably directly produces crossover products (Boddy et al. 2001) (i.e., it is a procrossover resolvase). Msh4 ...
Identification of Saccharomyces cerevisiae proteins that bind to
... NCP and acidic patch. A figure adapted from a paper by 9 Kalashnikova et al. (2013) showing the intrinsically unstructured domains protruding from the NCP core opposed to the globular domain around which the DNA is wrapped. The patch of acidic residues in the H2A-H2B dimer has been shown to play an ...
... NCP and acidic patch. A figure adapted from a paper by 9 Kalashnikova et al. (2013) showing the intrinsically unstructured domains protruding from the NCP core opposed to the globular domain around which the DNA is wrapped. The patch of acidic residues in the H2A-H2B dimer has been shown to play an ...
The green fluorescent protein: discovery
... threading α-helix. The N-terminal residue and the C-terminal residues 230-238, approximately corresponding to the maximal numbers of residues that can be removed from the N(2 residues) and C-terminal (6 residues) respectively of GFP at retained fluorescence, are disordered and therefore unresolved i ...
... threading α-helix. The N-terminal residue and the C-terminal residues 230-238, approximately corresponding to the maximal numbers of residues that can be removed from the N(2 residues) and C-terminal (6 residues) respectively of GFP at retained fluorescence, are disordered and therefore unresolved i ...
Detergents and Surfactants
... agent, although in essence, no neutralisation takes place in the manufacturing process. In addition to being good cleansing agents, www.healthoracle.org ...
... agent, although in essence, no neutralisation takes place in the manufacturing process. In addition to being good cleansing agents, www.healthoracle.org ...
FtsZ - Cytoskeleton, Inc.
... proteins involved in bacterial cell division. Besides serving as a scaffold for the other cell division proteins, FtsZ itself may exert cytokinetic forces that lead to cell division (1-6). FtsZ is considered a prokaryotic homolog to the eukaryotic protein tubulin (5-11). Both tubulin and FtsZ contai ...
... proteins involved in bacterial cell division. Besides serving as a scaffold for the other cell division proteins, FtsZ itself may exert cytokinetic forces that lead to cell division (1-6). FtsZ is considered a prokaryotic homolog to the eukaryotic protein tubulin (5-11). Both tubulin and FtsZ contai ...
The Fusion Core Complex of the Peste des Petits Ruminants Virus Is
... function mediated by HN or H protein (1-13). The F proteinmediated fusion process has been extensively investigated in other paramyxoviruses (14). In general, it is synthesized as a precursor (F0) which is cleaved by the host machinery into two fragments, F1 and F2, which are linked by a disulfide b ...
... function mediated by HN or H protein (1-13). The F proteinmediated fusion process has been extensively investigated in other paramyxoviruses (14). In general, it is synthesized as a precursor (F0) which is cleaved by the host machinery into two fragments, F1 and F2, which are linked by a disulfide b ...
How does photosystem 2 split water?
... dimeric PS2 particles in the membrane underneath. This finding suggests that the PBS-PS2 supercomplexes are composed of two PS2 complexes and one PBS7; an arrangement that is also confirmed by biochemical analyses and energy transfer studies 29. Also, the two basal PBS core cylinders that are respon ...
... dimeric PS2 particles in the membrane underneath. This finding suggests that the PBS-PS2 supercomplexes are composed of two PS2 complexes and one PBS7; an arrangement that is also confirmed by biochemical analyses and energy transfer studies 29. Also, the two basal PBS core cylinders that are respon ...
chemistry (9189)
... Experimental skills and investigations. This paper is designed to test appropriate aspects of objectives C1 to C6. The practical paper may also involve some calculations based on experimental results. The Subject Core requires some assessment of planning/design skills – a relatively structured examp ...
... Experimental skills and investigations. This paper is designed to test appropriate aspects of objectives C1 to C6. The practical paper may also involve some calculations based on experimental results. The Subject Core requires some assessment of planning/design skills – a relatively structured examp ...
biomolecules (introduction, structure
... Here, we observe that the amino group on the asymmetric carbon atom of alanine can be stereochemically related to the substituent hydroxyl group on the asymmetric carbon atom of glyceraldehyde, the carboxyl group of alanine can be related to the aldehyde group of glyceraldehyde, and the R group of a ...
... Here, we observe that the amino group on the asymmetric carbon atom of alanine can be stereochemically related to the substituent hydroxyl group on the asymmetric carbon atom of glyceraldehyde, the carboxyl group of alanine can be related to the aldehyde group of glyceraldehyde, and the R group of a ...
Functional and structural studies of a C
... Fig. 1.1 The ribosomal protein synthesis cycle. Initiation occurs by two ribosomal subunits being assembled at the start codon of an mRNA molecule together with a loaded tRNA molecule and three initiation factors (IF), and the first peptide bond is formed. The sequence of events that results in the ...
... Fig. 1.1 The ribosomal protein synthesis cycle. Initiation occurs by two ribosomal subunits being assembled at the start codon of an mRNA molecule together with a loaded tRNA molecule and three initiation factors (IF), and the first peptide bond is formed. The sequence of events that results in the ...
Cyclol
The cyclol hypothesis is the first structural model of a folded, globular protein. It was developed by Dorothy Wrinch in the late 1930s, and was based on three assumptions. Firstly, the hypothesis assumes that two peptide groups can be crosslinked by a cyclol reaction (Figure 1); these crosslinks are covalent analogs of non-covalent hydrogen bonds between peptide groups. These reactions have been observed in the ergopeptides and other compounds. Secondly, it assumes that, under some conditions, amino acids will naturally make the maximum possible number of cyclol crosslinks, resulting in cyclol molecules (Figure 2) and cyclol fabrics (Figure 3). These cyclol molecules and fabrics have never been observed. Finally, the hypothesis assumes that globular proteins have a tertiary structure corresponding to Platonic solids and semiregular polyhedra formed of cyclol fabrics with no free edges. Such ""closed cyclol"" molecules have not been observed either.Although later data demonstrated that this original model for the structure of globular proteins needed to be amended, several elements of the cyclol model were verified, such as the cyclol reaction itself and the hypothesis that hydrophobic interactions are chiefly responsible for protein folding. The cyclol hypothesis stimulated many scientists to research questions in protein structure and chemistry, and was a precursor of the more accurate models hypothesized for the DNA double helix and protein secondary structure. The proposal and testing of the cyclol model also provides an excellent illustration of empirical falsifiability acting as part of the scientific method.