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A (1,1) - Math.Cinvestav
A (1,1) - Math.Cinvestav

... conformations of a protein macromolecule. A conformation is understood as the spatial arrangement of all “elementary parts” of a macromolecule. Atoms, units of a polymer chain, or even larger molecular fragments of a chain can be considered as its “elementary parts”. Particular representation depend ...
procite - UWI St. Augustine
procite - UWI St. Augustine

... [G is the N-myristoylation site] -Note: We deliberately include as potential myristoylated glycine residues, those which are internal to a sequence. It could well be that the sequence under study represents a viral polyprotein precursor and that ...
Mechanism of Posttranslational Regulation of Phenol
Mechanism of Posttranslational Regulation of Phenol

... over 95% or higher. Extra high purity PST was used for the extraction of nucleotide to prevent any possible contamination. Purified PST was further passed through a HiTrap column with desalting buffer (100 mM bis-tris propane at pH 7.0, 1 mM EDTA, 10% glycerol (v/v), and 125 mM sucrose) to remove an ...
Investigations of non-enzymatic glycation with new bioanalytical
Investigations of non-enzymatic glycation with new bioanalytical

... Desalting of proteins and peptides by solid phase extraction (SPE) Solid phase extraction (SPE) is an easily applicable method most frequently used for desalting, purification, preconcentration and fractionation of a complex mixture of peptides. Fractionation prior to analyses can have a significant ...
Systematic Name Gene Name Motif ID Expert Confidence Dubious
Systematic Name Gene Name Motif ID Expert Confidence Dubious

... None of the motifs agree with each other. The literature motif characterization was indirect; hence low confidence that this is the true motif. The ChIP-chip motifs score higher on ChIP data but that's circular. PBM and ChIP-chip motifs agree; select ChIP-chip as it scores higher on ChIP-chip althou ...
ATP– and ADP–DnaA protein, a molecular switch in gene regulation
ATP– and ADP–DnaA protein, a molecular switch in gene regulation

... In order to define whether this lack of repression was due to the ADP–DnaA/ATP–DnaA switch, we analyzed a DNA fragment containing ATP–DnaA boxes a, b, c and DnaA boxes 1 and 2 in the BIAcore. Binding of ATP– and ADP–DnaA protein to a wild-type and a dnaA820 DNA fragment was tested. In addition, we t ...
Identification and first characterization of pairing
Identification and first characterization of pairing

... Cross-mating between adults of different schistosome species indicated that there are different stimuli for female development and size (Basch & Basch, 1984; Armstrong, 1965). Initial pairing was hypothesized to be a result of random encounters followed by tactile stimulation rather than a hormonal ...
Identification and Cloning of a Cryptococcal Deacetylase That
Identification and Cloning of a Cryptococcal Deacetylase That

C/EBPα triggers proteasome‐dependent degradation of cdk4 during
C/EBPα triggers proteasome‐dependent degradation of cdk4 during

43803_C000 1..20
43803_C000 1..20

... Growth Hormone and Athletic Performance ...........................................................................28 Problems with Exogenous Growth Hormone ..........................................................................30 Effects of Growth Hormone on Body Composition and Athletic Perform ...
STUDIES ON THE CHLORAMPHENICOL HALOGENASE CmlS
STUDIES ON THE CHLORAMPHENICOL HALOGENASE CmlS

... have grown more during these past 2 years under your supervision that I would have thought possible. Thank you for helping me get through what seemed, at times, impossible. I would also like to thank Dr. Derek Pratt for answering any and every question I had whether it was geared towards organic che ...
{PDOC00000} {BEGIN
{PDOC00000} {BEGIN

... dehydrogenase (complex I). - Rhizobium nodulation protein nodF, which probably acts as an ACP in the synthesis of the nodulation Nod factor fatty acyl chain. The sequence around the phosphopantetheine attachment site is conserved in all these proteins and can be used as a signature pattern. -Consens ...
PDF - Beilstein
PDF - Beilstein

... peptide NisA showed that dehydration involves the glutamylation of Ser and Thr side chains prior to elimination of glutamate [50]. This mechanistic proposal was established due to the observation that three NisB mutants (R786A, R826A and H961A) were able to transfer multiple glutamates to NisA witho ...
Systems Biotechnology of Pseudomonas putida for the enhanced
Systems Biotechnology of Pseudomonas putida for the enhanced

... Oil-based plastic production processes have given rise to several environmental problems and energy availability. Over the past 30 years, polyhydroxyalkanoates (PHA) have become one of the main sustainable alternatives to replace petroleum-base commodities. These biopolymers have superior features t ...
Serine Residues 286, 288, and 293 within the CIITA: A
Serine Residues 286, 288, and 293 within the CIITA: A

PKC regulates the hypertrophic growth of cardiomyocytes through
PKC regulates the hypertrophic growth of cardiomyocytes through

New Generation Collagens
New Generation Collagens

... resistant gel, makes it possible to emulsify with many other biologically active substances that are beneficial to our skin. Collagen is a word that is synonymous with many restorative and rejuvenating products. That is why in the beauty and pharmaceutical world it is frequently misused. The name co ...
On the role and formation of covalently bound flavin cofactors Heuts
On the role and formation of covalently bound flavin cofactors Heuts

Sequence and Structure Classification of Kinases
Sequence and Structure Classification of Kinases

... implications. We predicted a number of hypothetical proteins in the database that may possess kinase activity. Furthermore, we performed a large-scale structural prediction for kinases with unknown structures. Such classification of protein families can be quite helpful in understanding relationship ...
Lipoprotein structure
Lipoprotein structure

... of the neutral lipids, including small amounts of dissolved unesterified cholesterol (about 1/3 of the total) and other lipophilic molecules. In HDL and VLDL, the core lipids do not appear to be organized because of the small volume available in HDL, and because of the high content of fluid triacylg ...
Technical Brief
Technical Brief

... The term kwashiorkor was introduced in the medical literature by Cicely Williams, a Jamaican physician working in what is now Ghana in an article published in the Lancet in 1935 1 (republished in 20032). It is derived from a word of the Krobo language from Ghana and refers to a child displaced from ...
Identification of a Nuclear Export Signal in the Catalytic Subunit of
Identification of a Nuclear Export Signal in the Catalytic Subunit of

The Copper Efflux Regulator CueR Is Subject to ATP
The Copper Efflux Regulator CueR Is Subject to ATP

... transformations in the DNA conformation (Changela et al., 2003; Chen et al., 2003; Stoyanov and Brown, 2003; Philips et al., 2015). By kinks and undertwisting, the DNA switches from a B-form into an A-form-like conformation that allows access of the RNA polymerase. The metal-free CueR dimer (apo-Cue ...
please refer to anzfa`s guide to applications and proposals for a
please refer to anzfa`s guide to applications and proposals for a

... FSANZ invites public comment on this Report based on regulation impact principles for the purpose of preparing an amendment to the Code for approval by the FSANZ Board. Written submissions are invited from interested individuals and organisations to assist FSANZ in further considering this Applicati ...
New insights into regulation of p53 protein degradation
New insights into regulation of p53 protein degradation

... Modifications of p53 regulates p53 degradation through ubiquitination-dependent proteasome pathway Covalent modifications of p53 occur on more than 40 different amino acid residues and lead to different p53 activation. These modifications include phosphorylation, acetylation, methylation, ubiquitina ...
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Nuclear magnetic resonance spectroscopy of proteins



Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.
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