Affinity Precipitation of a Monoclonal Antibody ELP-Z Stimuli Responsive Biopolymer
... purification of target proteins by fusing them to ELPs via a self-splicing intein domain which eliminated the need for a proteolytic cleavage step. However, this approach can result in relatively low titers making it less amenable for large scale mAb production and purification. Kim et al. (2005) have ...
... purification of target proteins by fusing them to ELPs via a self-splicing intein domain which eliminated the need for a proteolytic cleavage step. However, this approach can result in relatively low titers making it less amenable for large scale mAb production and purification. Kim et al. (2005) have ...
ldentification of Surface-Exposed Domains on the Reducing Side of
... initially identified by western blotting (data not shown), we determined amino acid sequences of their N termini for accurate identification and mapping (Table H). These studies revealed that all sequenced cleavage products were derived from PsaD, PsaE, and PsaF. In contrast, PsaL and PsaK did not d ...
... initially identified by western blotting (data not shown), we determined amino acid sequences of their N termini for accurate identification and mapping (Table H). These studies revealed that all sequenced cleavage products were derived from PsaD, PsaE, and PsaF. In contrast, PsaL and PsaK did not d ...
evaluation of l-methionine bioavailability in nursery pigs
... utilized by animals for protein synthesis, L-Met could, theoretically, be more available. Four experiments were conducted to evaluate L-Met bioavailability in nursery pigs with 21-day growth trials. A total of 105,105,112 and 84 crossbred pigs were used in Exp. 1, 2, 3 and 4, respectively. Each expe ...
... utilized by animals for protein synthesis, L-Met could, theoretically, be more available. Four experiments were conducted to evaluate L-Met bioavailability in nursery pigs with 21-day growth trials. A total of 105,105,112 and 84 crossbred pigs were used in Exp. 1, 2, 3 and 4, respectively. Each expe ...
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... lamins. Thus the CeLam-1 gene of the nematode Caenorhabditis elegans encodes a protein with a nuclear localization signal and a CaaX box. However, this lamin is atypical since it lacks the consensus cdc2 phosphorylation site in the head domain (Riemer et al., 1993). An extensive biochemical and immu ...
... lamins. Thus the CeLam-1 gene of the nematode Caenorhabditis elegans encodes a protein with a nuclear localization signal and a CaaX box. However, this lamin is atypical since it lacks the consensus cdc2 phosphorylation site in the head domain (Riemer et al., 1993). An extensive biochemical and immu ...
Nuclear export signal located within the DNAbinding domain of the
... 369±436 contains a functional NES, we evaluated whether this region could promote nuclear exclusion of a heterologous protein, GFP. The small size of GFP, ~27 kDa, and the lack of an endogenous NLS or NES allows the protein to distribute between the nuclear and cytoplasmic compartments (Figure 4A). ...
... 369±436 contains a functional NES, we evaluated whether this region could promote nuclear exclusion of a heterologous protein, GFP. The small size of GFP, ~27 kDa, and the lack of an endogenous NLS or NES allows the protein to distribute between the nuclear and cytoplasmic compartments (Figure 4A). ...
Ser/Thr
... Addition of GlcNac b2 to O-Man Glycosyltransferase like protein Glycosyltransferase like Golgi protein ...
... Addition of GlcNac b2 to O-Man Glycosyltransferase like protein Glycosyltransferase like Golgi protein ...
History of the Glycosaminoglycan Symposia in the Scientific Context
... – Alternately, autolysis or a salt extraction can be performed. ...
... – Alternately, autolysis or a salt extraction can be performed. ...
allyl cysteine sulphoxide
... callus have been maintained for up to 15 days on a phytogel/MS medium, with and without sulphate, containing a range of potential precursors to the synthesis of Alliin (at different concentrations). This method of substrate feeding will only give a positive result if: the substrate gets into the ...
... callus have been maintained for up to 15 days on a phytogel/MS medium, with and without sulphate, containing a range of potential precursors to the synthesis of Alliin (at different concentrations). This method of substrate feeding will only give a positive result if: the substrate gets into the ...
The G-protein regulator LGN modulates the activity of the NO
... Indicated amounts of purified LGN and sGC proteins were incubated for 10 min at room temperature with 20 μl of control buffer (2.5 mg/ml albumin, 50 mM TEA and 100 μM EGTA) or 50 μg of COS-7 cell lysate. Following the incubation, the sample was mixed with 40 μl of reaction buffer [125 mM TEA, 250 μM ...
... Indicated amounts of purified LGN and sGC proteins were incubated for 10 min at room temperature with 20 μl of control buffer (2.5 mg/ml albumin, 50 mM TEA and 100 μM EGTA) or 50 μg of COS-7 cell lysate. Following the incubation, the sample was mixed with 40 μl of reaction buffer [125 mM TEA, 250 μM ...
Identification and characterization of novel interaction
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
... In Gram-positiven Bakterien mit niedrigem GC-Gehalt, wie Bacillus subtilis, wird KohlenstoffKatabolitenregulation überwiegend vom Katabolit Kontroll Protein A (CcpA), einem globalen Transkriptionsregulator, vermittelt. CcpA reprimiert oder aktiviert die Expression von hunderten von ...
Propionate stimulates pyruvate oxidation in the - AJP
... addition to the dominant glutamate resonances, the spectra also show resonances of malate, citrate, and aspartate, with malate and citrate being higher when propionate is supplied (Fig. 1B). The insets in Fig. 1 show the glutamate C2, C3, and C4 resonances. Isotopomers of glutamate that have three o ...
... addition to the dominant glutamate resonances, the spectra also show resonances of malate, citrate, and aspartate, with malate and citrate being higher when propionate is supplied (Fig. 1B). The insets in Fig. 1 show the glutamate C2, C3, and C4 resonances. Isotopomers of glutamate that have three o ...
Schubert, C. M., R. Lin, C. J. de Vries, R. H. A.
... and PAR-2 localize to the posterior cortex of the embryo (Guo and Kemphues, 1995; Boyd et al., 1996) and PAR-3, PKC-3, and PAR-6 localize to the anterior cortex (Etemad-Moghadam et al., 1995; Tabuse et al., 1998; Hung and Kemphues, 1999). We refer here to these proteins collectively as PAR proteins. ...
... and PAR-2 localize to the posterior cortex of the embryo (Guo and Kemphues, 1995; Boyd et al., 1996) and PAR-3, PKC-3, and PAR-6 localize to the anterior cortex (Etemad-Moghadam et al., 1995; Tabuse et al., 1998; Hung and Kemphues, 1999). We refer here to these proteins collectively as PAR proteins. ...
Phylogenetic Classification of Protozoa Based on the Structure of
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
... DHFR-TS proteins, notably from Apicomplexan protozoa, include a long linker between the DHFR and TS domains. The linker polypeptide, even within the Apicomplexans, varies significantly in length; the entire linker in C. hominis is 58 amino acids, the linker in Toxoplasma gondii is 72 amino acids, an ...
Phylogenetic Classification of Protozoa Based on the
... structure factors with Truncate (13). A random set of reflections (10%) was set aside for the calculation of Rfree. Structure Determination—The structure was determined by molecular replacement using a model of thymidylate synthase from P. carinii (Protein Data Bank 1F28) (14), from which all ligand ...
... structure factors with Truncate (13). A random set of reflections (10%) was set aside for the calculation of Rfree. Structure Determination—The structure was determined by molecular replacement using a model of thymidylate synthase from P. carinii (Protein Data Bank 1F28) (14), from which all ligand ...
CHAPTER 1 INTRODUCTION
... (Reichert, 1991). Soya bean also contains about 20 % oil, which is very desirable because it contains a large proportion of unsaturated fatty acids (Ologhobo , 1989). Increased yields of soya, coupled with advances in processing proteins from the soya bean, have improved the opportunity for the furt ...
... (Reichert, 1991). Soya bean also contains about 20 % oil, which is very desirable because it contains a large proportion of unsaturated fatty acids (Ologhobo , 1989). Increased yields of soya, coupled with advances in processing proteins from the soya bean, have improved the opportunity for the furt ...
2.2. Garrido-Franco, M. Structure E. coli
... closed state. All related PNP synthases are predicted to fold into a similar TIM barrel pattern and have comparable active site architecture. Thus, a common mechanism can be anticipated. ...
... closed state. All related PNP synthases are predicted to fold into a similar TIM barrel pattern and have comparable active site architecture. Thus, a common mechanism can be anticipated. ...
Organization of Physical Interactomes as
... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
papain, a plant enzyme of biological importance
... terminal portion. As this occurs throughout the peptide chains of the protein, the protein breaks apart. The mechanism by which it breaks peptide bonds involves deprotonation of Cys-25 by His-159. Asparagine-175 helps to orient the imidazole ring of His-159 to allow this deprotonation to take place. ...
... terminal portion. As this occurs throughout the peptide chains of the protein, the protein breaks apart. The mechanism by which it breaks peptide bonds involves deprotonation of Cys-25 by His-159. Asparagine-175 helps to orient the imidazole ring of His-159 to allow this deprotonation to take place. ...
Barley Aleurone Cells Contain Two Types of
... aleurone cells and are present at grain maturity. They contain numerous inclusions of phytin and protein/carbohydrate (Figures 1A and 1E; Jacobsen et al., 1971) and are delineated by a tonoplast that has oleosomes embedded between the inner and outer leaflets of the membrane (Fernandez and Staehelin ...
... aleurone cells and are present at grain maturity. They contain numerous inclusions of phytin and protein/carbohydrate (Figures 1A and 1E; Jacobsen et al., 1971) and are delineated by a tonoplast that has oleosomes embedded between the inner and outer leaflets of the membrane (Fernandez and Staehelin ...
Diacylglycerol kinase zeta in hypothalamus interacts with long form leptin receptor. Relation to dietary fat and body weight regulation
... development of sensory neurons and regions undergoing apoptosis (13). Furthermore, DGK may also participate as a key enzyme in the biosynthesis of complex lipids. This is suggested by the fact that DGK is widely and abundantly expressed throughout the body (14) and that it can promiscuously use va ...
... development of sensory neurons and regions undergoing apoptosis (13). Furthermore, DGK may also participate as a key enzyme in the biosynthesis of complex lipids. This is suggested by the fact that DGK is widely and abundantly expressed throughout the body (14) and that it can promiscuously use va ...
Raines, ChemRev 1998
... for which a correct amino acid sequence was determined,52,53 and the third enzyme and fourth protein (after myoglobin,54,55 lysozyme,56 and carboxypeptidase A57) whose three-dimensional structure was determined by X-ray diffraction analysis.58 A general method for using fast atom bombardment mass sp ...
... for which a correct amino acid sequence was determined,52,53 and the third enzyme and fourth protein (after myoglobin,54,55 lysozyme,56 and carboxypeptidase A57) whose three-dimensional structure was determined by X-ray diffraction analysis.58 A general method for using fast atom bombardment mass sp ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.