![HEMOSTASIS](http://s1.studyres.com/store/data/008289919_1-bc40ce2d3d986adb9e3b48ece64a00c7-300x300.png)
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain
... Lys content. Since RTA expression was known to be toxic to plant cells (Frigerio et al., 1998), and since this toxicity may somehow affect degradation kinetics, all these changes were introduced in the background of an RTA active site mutant in which E177 was changed to Asp. This mutant (RTA*) is ch ...
... Lys content. Since RTA expression was known to be toxic to plant cells (Frigerio et al., 1998), and since this toxicity may somehow affect degradation kinetics, all these changes were introduced in the background of an RTA active site mutant in which E177 was changed to Asp. This mutant (RTA*) is ch ...
original version
... binding and hydrolysis (see sections 2 and 3). Yet, DnaA(L366K) can initiate replication only when a limited amount of wild-type DnaA is present [69]. In agreement, nucleoprotein complexes (see section 2) containing only DnaA(L366K) protein were found inefficient at unwinding DNA duplex at oriC, and ...
... binding and hydrolysis (see sections 2 and 3). Yet, DnaA(L366K) can initiate replication only when a limited amount of wild-type DnaA is present [69]. In agreement, nucleoprotein complexes (see section 2) containing only DnaA(L366K) protein were found inefficient at unwinding DNA duplex at oriC, and ...
Full-Text PDF
... binding and hydrolysis (see sections 2 and 3). Yet, DnaA(L366K) can initiate replication only when a limited amount of wild-type DnaA is present [69]. In agreement, nucleoprotein complexes (see section 2) containing only DnaA(L366K) protein were found inefficient at unwinding DNA duplex at oriC, and ...
... binding and hydrolysis (see sections 2 and 3). Yet, DnaA(L366K) can initiate replication only when a limited amount of wild-type DnaA is present [69]. In agreement, nucleoprotein complexes (see section 2) containing only DnaA(L366K) protein were found inefficient at unwinding DNA duplex at oriC, and ...
Investigation of Factors Affecting Opalescence and Phase
... separation (formation of protein-rich and protein-poor phase) is related to reduced solubility of the proteins.12 From a pharmaceutical point of view, liquid-liquid phase separation (LLPS) is a concern for formulation development since various protein solutions are stored at refrigerated condition ...
... separation (formation of protein-rich and protein-poor phase) is related to reduced solubility of the proteins.12 From a pharmaceutical point of view, liquid-liquid phase separation (LLPS) is a concern for formulation development since various protein solutions are stored at refrigerated condition ...
Identification of the Factors Responsible for the Interaction of
... Abstract: Hsp90 is a stress protein that acts as a molecular chaperone and is known to assist in the maturation, folding and stabilization of various cellular proteins known as ‘client proteins’. However, the factors that drive the interaction between Hsp90 and its client proteins are not well und ...
... Abstract: Hsp90 is a stress protein that acts as a molecular chaperone and is known to assist in the maturation, folding and stabilization of various cellular proteins known as ‘client proteins’. However, the factors that drive the interaction between Hsp90 and its client proteins are not well und ...
Document
... •Hypotheses can never be proved; they can only be disproved. •A hypothesis that fails one or more tests is considered disproved and it is ...
... •Hypotheses can never be proved; they can only be disproved. •A hypothesis that fails one or more tests is considered disproved and it is ...
Recoding the Genetic Code with Selenocysteine
... produce therapeutic agents that specifically target mismodiand ICP-MS analysis independently confirmed 98.5 % Sec fied proteins, there is an urgent need to synthesize proteins incorporation in response to UGA and 95.1 % Sec insertion containing several different, distinct ncAAs. Progress in the for ...
... produce therapeutic agents that specifically target mismodiand ICP-MS analysis independently confirmed 98.5 % Sec fied proteins, there is an urgent need to synthesize proteins incorporation in response to UGA and 95.1 % Sec insertion containing several different, distinct ncAAs. Progress in the for ...
Ph.D. Thesis Azar Shahpiri
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
13C-fluxomics
... Link between metabolites turn-over & labelling incorporation Labelled substrate = 13C glucose • Isotopic Steady state of glycolytic intermediates: range of seconds – minutes • Isotopic Steady state of TCA intermediates: more than 30min • Isotopic Steady state of protein-bound AA: several hours ...
... Link between metabolites turn-over & labelling incorporation Labelled substrate = 13C glucose • Isotopic Steady state of glycolytic intermediates: range of seconds – minutes • Isotopic Steady state of TCA intermediates: more than 30min • Isotopic Steady state of protein-bound AA: several hours ...
DOCTORAL THESIS
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
... transglycosylase activity catalyzing cleavage and reconection of xyloglucan molecules, which is proposed to be involved in secondary cell wall morphogenesis. The creation of a family 16 carbohydrate active enzyme β-glucanase/XET hybrids were attempted in order to design a chimeric enzyme with one or ...
Cardiac mitochondrial matrix and respiratory complex - AJP
... (12, 13), and a mismatch of just a few percent between the production and utilization rates can result in major disruptions Address for reprint requests and other correspondence: R. S. Balaban, Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, 9000 ...
... (12, 13), and a mismatch of just a few percent between the production and utilization rates can result in major disruptions Address for reprint requests and other correspondence: R. S. Balaban, Laboratory of Cardiac Energetics, National Heart Lung and Blood Institute, 9000 ...
Basic Science for Clinicians
... Cells have evolved elaborate mechanisms to protect themselves against changes in their environment. In the heart, one of the most important forms of cellular stress is energy depletion from ischemia. ATP is the primary energy source for contractile proteins, ion pumps, and many enzymes. Normally, en ...
... Cells have evolved elaborate mechanisms to protect themselves against changes in their environment. In the heart, one of the most important forms of cellular stress is energy depletion from ischemia. ATP is the primary energy source for contractile proteins, ion pumps, and many enzymes. Normally, en ...
Eukaryote-Like Serine/Threonine Kinases and Phosphatases in
... FIG. 1. Structure of the Ser/Thr kinase catalytic domain. (A) Crystal structure of the mouse PKA catalytic domain in complex with an ATP molecule and an inhibitor peptide (Protein Data Bank [PDB] accession number 1ATP). The PKA N-terminal lobe is shown in gray, and the C-terminal lobe is shown in bl ...
... FIG. 1. Structure of the Ser/Thr kinase catalytic domain. (A) Crystal structure of the mouse PKA catalytic domain in complex with an ATP molecule and an inhibitor peptide (Protein Data Bank [PDB] accession number 1ATP). The PKA N-terminal lobe is shown in gray, and the C-terminal lobe is shown in bl ...
poultry meal vs poultry byproduct meal
... poultry ingredients are of equal quality. There are substantial differences between different grades within each of these ingredients. These differences may be due to differences in processing or to differences in the quality of the parts used to make the ingredient. The process used to produce both ...
... poultry ingredients are of equal quality. There are substantial differences between different grades within each of these ingredients. These differences may be due to differences in processing or to differences in the quality of the parts used to make the ingredient. The process used to produce both ...
Review Process - The EMBO Journal
... apparent opposite ways, causing an increase in axonal length (which was not explored further) and a loss of dendrite complexity. Furthermore, knockdown of TMEM106B induces a loss of synaptic proteins and alterations in spine morphology. Interestingly, the dendritic phenotype is phenocopied by overex ...
... apparent opposite ways, causing an increase in axonal length (which was not explored further) and a loss of dendrite complexity. Furthermore, knockdown of TMEM106B induces a loss of synaptic proteins and alterations in spine morphology. Interestingly, the dendritic phenotype is phenocopied by overex ...
A processed form of the Spätzle protein defines dorsal
... Perivitelline extracts from Toll− embryos did not provide enough material for more extensive biochemical characterization of the polarizing activity. Due to female sterility, Toll− flies cannot be grown as a homozygous population, and therefore must be generated by crosses. In addition, Toll has an ...
... Perivitelline extracts from Toll− embryos did not provide enough material for more extensive biochemical characterization of the polarizing activity. Due to female sterility, Toll− flies cannot be grown as a homozygous population, and therefore must be generated by crosses. In addition, Toll has an ...
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of
... bodies, we developed a novel pH-sensitive fluorescent tag consisting of a tandem fusion of the red, acid-insensitive mCherry and the acid-sensitive green fluorescent proteins. This approach revealed that p62- and LC3-positive bodies are degraded in autolysosomes. Strikingly, even rather large p62-po ...
... bodies, we developed a novel pH-sensitive fluorescent tag consisting of a tandem fusion of the red, acid-insensitive mCherry and the acid-sensitive green fluorescent proteins. This approach revealed that p62- and LC3-positive bodies are degraded in autolysosomes. Strikingly, even rather large p62-po ...
Full-Text PDF
... destroys the IS; and (3) in order to acquire sufficient neutralizing capacity, HIV-1 Abs need a much longer affinity maturation process than do Abs elicited by other viruses, with the result that protective Abs usually appear only after about two years [28]. It is not possible during such a long Ab ...
... destroys the IS; and (3) in order to acquire sufficient neutralizing capacity, HIV-1 Abs need a much longer affinity maturation process than do Abs elicited by other viruses, with the result that protective Abs usually appear only after about two years [28]. It is not possible during such a long Ab ...
Lactobacillus plantarum - UEF Electronic Publications
... environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, makin ...
... environmental niches due to their large adaptation capacity. They are responsible for the fermentation of many fermented food and feed products because they can transform sugars into organic acids and decrease the pH of the medium. This acidification prevents the growth of pathogenic bacteria, makin ...
Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as
... competent for cargo collection once they come to a halt on an ERES upon transient detachment from actin. An alternative model envisages that individual Golgi bodies move to continually collect vesicles budding from the ER (vacuum cleaner model; Boevink et al., 1998). This would suggest that the enti ...
... competent for cargo collection once they come to a halt on an ERES upon transient detachment from actin. An alternative model envisages that individual Golgi bodies move to continually collect vesicles budding from the ER (vacuum cleaner model; Boevink et al., 1998). This would suggest that the enti ...
Isolation of Casein, Lactose, and Albumin from Milk
... Additional lipids (fats and oils) in milk include small amounts of cholesterol, phospholipids, and lecithins (phospholipids conjugated with choline). The structures of phospholipids and lecithins are shown. The phospholipids help to stabilize the whole milk emulsion; the phosphate groups help to ach ...
... Additional lipids (fats and oils) in milk include small amounts of cholesterol, phospholipids, and lecithins (phospholipids conjugated with choline). The structures of phospholipids and lecithins are shown. The phospholipids help to stabilize the whole milk emulsion; the phosphate groups help to ach ...
The DELLA protein family and gibberellin signal transduction
... system. However, the DELLA proteins are also post-translationally modified which affects their activity. It is believed the DELLAs are modified both with O-linked N-Acetyl glucosamine for stability and phosphate groups to mark them for F-box recognition. However, the precise nature, and role of thes ...
... system. However, the DELLA proteins are also post-translationally modified which affects their activity. It is believed the DELLAs are modified both with O-linked N-Acetyl glucosamine for stability and phosphate groups to mark them for F-box recognition. However, the precise nature, and role of thes ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.