Structure of a Pheromone Receptor-Associated MHC Molecule with
... [36]. M10.5 omit electron-density maps in which the a1 and a2 helices were removed from structure factor calculations return clear density for the omitted region (Figure 1B), indicating that the maps are of sufficient quality to detect bound molecules of the size of an 8–10 residue peptide. However, ...
... [36]. M10.5 omit electron-density maps in which the a1 and a2 helices were removed from structure factor calculations return clear density for the omitted region (Figure 1B), indicating that the maps are of sufficient quality to detect bound molecules of the size of an 8–10 residue peptide. However, ...
Virulence Factor Ontology
... Compare novel strains to what? • Very few manual annotations available for prokaryotes, especially in public databases like NCBI and UniProt Table 1. Percentage of genes in UniProt with functional assignments to Gene Ontology terms based on experimental evidence in the primary literature. ...
... Compare novel strains to what? • Very few manual annotations available for prokaryotes, especially in public databases like NCBI and UniProt Table 1. Percentage of genes in UniProt with functional assignments to Gene Ontology terms based on experimental evidence in the primary literature. ...
Of earwax and migration
... choreoathetosis – which caused her extremities to make uncontrollable twisting movements. This woman had wet earwax, as did six other members of her family, which is an unusual occurrence in Japan. A number of scientists also suggest a possible association of earwax type with armpit odour and even b ...
... choreoathetosis – which caused her extremities to make uncontrollable twisting movements. This woman had wet earwax, as did six other members of her family, which is an unusual occurrence in Japan. A number of scientists also suggest a possible association of earwax type with armpit odour and even b ...
post- translational modifications
... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
... for the synthesis of PTM peptides and proteins. Post-translational modifications (PTMs) enable the cell to change the properties and activities of proteins after expression. Most of the PTMs are catalyzed by specialized enzymes. Practically all proteinogenic amino acids can be modified, thus PTMs co ...
Toxoplasma gondii Chitinase Induces Macrophage Activation
... from T. gondii, and also revealed a region with possible homology with a putative CRD from B. subtilis [28]. This suggests that Tg_chitinase and paracoccin have similar features. Nonetheless, additional studies are required to elucidate the domains of Tg_chitinase. Indeed, NAGase activity was previo ...
... from T. gondii, and also revealed a region with possible homology with a putative CRD from B. subtilis [28]. This suggests that Tg_chitinase and paracoccin have similar features. Nonetheless, additional studies are required to elucidate the domains of Tg_chitinase. Indeed, NAGase activity was previo ...
Ribosomal Stalk Protein L12: Structure, Function and
... Each ribosomal subunit has a unique structure with some ‘landmark’ features on them. The 30S subunit has a relatively simple structure. The upper part of 30S is called the ‘head’, which is connected through a ‘neck’ to the lower part or ‘body’ carrying a shoulder (Figure 1). The 50S subunit presents ...
... Each ribosomal subunit has a unique structure with some ‘landmark’ features on them. The 30S subunit has a relatively simple structure. The upper part of 30S is called the ‘head’, which is connected through a ‘neck’ to the lower part or ‘body’ carrying a shoulder (Figure 1). The 50S subunit presents ...
PDF Full-text
... the microelectrode when the adsorbent consisted of PS microbeads with a SPG-modified surface. A different protein immobilization method involves using a protein implanted with a ligand that acts as a tag [9]. The tag consisted of a continuous sequence of seven to eight histidine residues and could b ...
... the microelectrode when the adsorbent consisted of PS microbeads with a SPG-modified surface. A different protein immobilization method involves using a protein implanted with a ligand that acts as a tag [9]. The tag consisted of a continuous sequence of seven to eight histidine residues and could b ...
Supplemental Table 3
... two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions [goid 8237] [evidence IEA]; Interacting selectively with zinc (Zn) ions [goid 8270] [evidence IEA]; Catalysis of a biochemical reaction at physiological temperatures. In biologically ca ...
... two metal ions hold the water molecule in place, and charged amino acid side chains are ligands for the metal ions [goid 8237] [evidence IEA]; Interacting selectively with zinc (Zn) ions [goid 8270] [evidence IEA]; Catalysis of a biochemical reaction at physiological temperatures. In biologically ca ...
THE MULTIFARIOUS AND DYNAMIC REGULATION OF THE LIVING CELL Karen van Eunen
... 1.2.1 Mechanisms of flux regulation The flux through a pathway or an enzyme can be changed by many different regulatory mechanisms. Speaking in the terms introduced above, many buttons are available in the cell. In this section I will describe the various levels of regulation briefly. The regulatory ...
... 1.2.1 Mechanisms of flux regulation The flux through a pathway or an enzyme can be changed by many different regulatory mechanisms. Speaking in the terms introduced above, many buttons are available in the cell. In this section I will describe the various levels of regulation briefly. The regulatory ...
Gene Section FST (follistatin) Atlas of Genetics and Cytogenetics
... et al., 1988) the first two, but not the third, are necessary for activin A binding (Keutmann et al., 2004; Harrington et al., 2006). Aside from activins, follistatin also binds several bone morphogenetic proteins (BMP) including BMP2, BMP4, BMP6 and BMP7 (Iemura et al., 1998; Glister et al., 2004). ...
... et al., 1988) the first two, but not the third, are necessary for activin A binding (Keutmann et al., 2004; Harrington et al., 2006). Aside from activins, follistatin also binds several bone morphogenetic proteins (BMP) including BMP2, BMP4, BMP6 and BMP7 (Iemura et al., 1998; Glister et al., 2004). ...
A motif and amino acid bias bioinformatics
... Downloaded from on June 17, 2017 - Published by www.plantphysiol.org Copyright © 2017 American Society of Plant Biologists. All rights reserved. ...
... Downloaded from on June 17, 2017 - Published by www.plantphysiol.org Copyright © 2017 American Society of Plant Biologists. All rights reserved. ...
Discrete Domains Mediate the Light-Responsive
... may have contributed to their nuclear exclusion. As the next step, we attempted to separate the core CLS from the domain causing insolubility. A fragment overlapping parts of the RING finger and helical domains (residues 67 to 177; Figures 7M to 7O) prevented the nuclear accumulation of NIa, while m ...
... may have contributed to their nuclear exclusion. As the next step, we attempted to separate the core CLS from the domain causing insolubility. A fragment overlapping parts of the RING finger and helical domains (residues 67 to 177; Figures 7M to 7O) prevented the nuclear accumulation of NIa, while m ...
Disruption of somitogenesis by a novel dominant allele of Lfng
... Fig. 2. Lfng expression and Notch activation are perturbed in LfngRLFNG/+ embryos. (A) Lfng expression in 10.5 dpc wild-type embryos oscillates (a-c; n=4/13 phase 1, 5/13 phase 2, 4/13 phase 3), but is reduced in the posterior PSM of LfngRLFNG/+ embryos (d; n=10). At 8.5 dpc cyclic Lfng expression i ...
... Fig. 2. Lfng expression and Notch activation are perturbed in LfngRLFNG/+ embryos. (A) Lfng expression in 10.5 dpc wild-type embryos oscillates (a-c; n=4/13 phase 1, 5/13 phase 2, 4/13 phase 3), but is reduced in the posterior PSM of LfngRLFNG/+ embryos (d; n=10). At 8.5 dpc cyclic Lfng expression i ...
Functional characterization of the Mycobacterium
... standard deviation was defined as the standard deviation of the 10 % of the lowest signals (about 397 spots). Only signals higher than 36(background standard deviation) were listed as detectable in the ‘simple detectable’ sheet. Spot CV was defined as the CV within a spot after its intensity and sta ...
... standard deviation was defined as the standard deviation of the 10 % of the lowest signals (about 397 spots). Only signals higher than 36(background standard deviation) were listed as detectable in the ‘simple detectable’ sheet. Spot CV was defined as the CV within a spot after its intensity and sta ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
... Lcm as a stru ctu ral protein o f the core, as the a n ch o r protein to the thylakoid m em brane, as a link er m odifying the spectral properties o f the asso ciated biliproteins, an d as the presum ed term inal em itter o f the phycobilisom e (R edlinger and G a n tt, 1982; R usckow ski an d Z ...
... Lcm as a stru ctu ral protein o f the core, as the a n ch o r protein to the thylakoid m em brane, as a link er m odifying the spectral properties o f the asso ciated biliproteins, an d as the presum ed term inal em itter o f the phycobilisom e (R edlinger and G a n tt, 1982; R usckow ski an d Z ...
Bacterial ribosome requires multiple L12 dimers for efficient initiation
... L12 proteins arranged as dimers on the protein L10 (1,2). The L10 protein binds to ribosomal RNA (rRNA) (nucleotides 1030–1124 in Escherichia coli) next to the protein L11 at the base of the stalk (3,4). L12 is the only multicopy protein on the ribosome (5), its number varying from species to specie ...
... L12 proteins arranged as dimers on the protein L10 (1,2). The L10 protein binds to ribosomal RNA (rRNA) (nucleotides 1030–1124 in Escherichia coli) next to the protein L11 at the base of the stalk (3,4). L12 is the only multicopy protein on the ribosome (5), its number varying from species to specie ...
CHRYSAORA QUINQUECIRRHA) Research Article
... Sadowska et al. [47] also observed that the share of collagen in total protein was considerably determined on the basis of hydroxyproline in samples. In this study, collagen obtained from the jellyfish could be possessed properties typical of type I collagen. The amino acid analyses of our collagena ...
... Sadowska et al. [47] also observed that the share of collagen in total protein was considerably determined on the basis of hydroxyproline in samples. In this study, collagen obtained from the jellyfish could be possessed properties typical of type I collagen. The amino acid analyses of our collagena ...
Linkage Novel Type of Antigen with an Isopeptide Peptide Splicing
... catalyzed by the proteasomal active sites. Transpeptidation reactions in the proteasome follow explicit rules and occur particularly efficiently when the C-terminal ligation partner contains a lysine or arginine residue at the site of ligation. Lysine contains two amino groups that theoretically may ...
... catalyzed by the proteasomal active sites. Transpeptidation reactions in the proteasome follow explicit rules and occur particularly efficiently when the C-terminal ligation partner contains a lysine or arginine residue at the site of ligation. Lysine contains two amino groups that theoretically may ...
A low-molecular-mass protein from Methylococcus
... Methylococcus capsulatus (Bath) and has been shown t o alter the substrate specificity and kinetics of NAD+-linked formaldehyde dehydrogenase (FDH) isolated from the same organism. Purification methods for both the modifin and FDH are presented which reliably produced pure protein for further analys ...
... Methylococcus capsulatus (Bath) and has been shown t o alter the substrate specificity and kinetics of NAD+-linked formaldehyde dehydrogenase (FDH) isolated from the same organism. Purification methods for both the modifin and FDH are presented which reliably produced pure protein for further analys ...
Genome-wide Investigation of Cellular Functions for tRNA Nucleus
... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
... biosynthesis, and leucine biosynthesis pathways, are primary targets of the tRNA trafficking processes. I confirmed the microarray data by both Northern and Western blot analyses. Levels of all tested target proteins involved in such amino acid biogenesis pathways are down-regulated when the tRNA n ...
LCAT activation by apoA-I in HDL3 exposed to HOCl or MPO
... MS/MS spectrum (unique peptides) Analysis Quantification Dual Statistical Criteria G-test and t-test ...
... MS/MS spectrum (unique peptides) Analysis Quantification Dual Statistical Criteria G-test and t-test ...
Nuclear magnetic resonance spectroscopy of proteins
Nuclear magnetic resonance spectroscopy of proteins (usually abbreviated protein NMR) is a field of structural biology in which NMR spectroscopy is used to obtain information about the structure and dynamics of proteins, and also nucleic acids, and their complexes. The field was pioneered by Richard R. Ernst and Kurt Wüthrich at the ETH, and by Ad Bax, Marius Clore and Angela Gronenborn at the NIH, among others. Structure determination by NMR spectroscopy usually consists of several phases, each using a separate set of highly specialized techniques. The sample is prepared, measurements are made, interpretive approaches are applied, and a structure is calculated and validated.NMR involves the quantum mechanical properties of the central core (""nucleus"") of the atom. These properties depend on the local molecular environment, and their measurement provides a map of how the atoms are linked chemically, how close they are in space, and how rapidly they move with respect to each other. These properties are fundamentally the same as those used in the more familiar Magnetic Resonance Imaging (MRI), but the molecular applications use a somewhat different approach, appropriate to the change of scale from millimeters (of interest to radiologists) to nano-meters (bonded atoms are typically a fraction of a nano-meter apart), a factor of a million. This change of scale requires much higher sensitivity of detection and stability for long term measurement. In contrast to MRI, structural biology studies do not directly generate an image, but rely on complex computer calculations to generate three-dimensional molecular models.Currently most samples are examined in a solution in water, but methods are being developed to also work with solid samples. Data collection relies on placing the sample inside a powerful magnet, sending radio frequency signals through the sample, and measuring the absorption of those signals. Depending on the environment of atoms within the protein, the nuclei of individual atoms will absorb different frequencies of radio signals. Furthermore the absorption signals of different nuclei may be perturbed by adjacent nuclei. This information can be used to determine the distance between nuclei. These distances in turn can be used to determine the overall structure of the protein.A typical study might involve how two proteins interact with each other, possibly with a view to developing small molecules that can be used to probe the normal biology of the interaction (""chemical biology"") or to provide possible leads for pharmaceutical use (drug development). Frequently, the interacting pair of proteins may have been identified by studies of human genetics, indicating the interaction can be disrupted by unfavorable mutations, or they may play a key role in the normal biology of a ""model"" organism like the fruit fly, yeast, the worm C. elegans, or mice. To prepare a sample, methods of molecular biology are typically used to make quantities by bacterial fermentation. This also permits changing the isotopic composition of the molecule, which is desirable because the isotopes behave differently and provide methods for identifying overlapping NMR signals.