Investigations of non-enzymatic glycation with new bioanalytical
... therefore, a value of approximately 8.2 was selected for this study. When the binding capacity of the tips was plotted against the ionic strength of the binding buffer, 150 mM ammonium chloride provided the best results. The effect of the pH of the eluent depicted in the function of the eluted gluco ...
... therefore, a value of approximately 8.2 was selected for this study. When the binding capacity of the tips was plotted against the ionic strength of the binding buffer, 150 mM ammonium chloride provided the best results. The effect of the pH of the eluent depicted in the function of the eluted gluco ...
Axonal of Kinesin in the Chain Isoforms
... 1985b; Vale et al., 1985) provided a candidate motor molecule for movement of membrane bounded organelles in FC, but questions remained about motors for SCa and SCb. One way of obtaining insights into the role of specific motor proteins in different axonal transport rate components is to characteriz ...
... 1985b; Vale et al., 1985) provided a candidate motor molecule for movement of membrane bounded organelles in FC, but questions remained about motors for SCa and SCb. One way of obtaining insights into the role of specific motor proteins in different axonal transport rate components is to characteriz ...
PDF - Beilstein
... are catalysed by distinct domains within NisB [52]. Protein homology analysis indicated that LanB-like proteins are widespread in bacteria [52], so this unusual use of an aminoacyltRNA may actually be common across nature. Interestingly, a subset of these proteins lack the elimination domain and are ...
... are catalysed by distinct domains within NisB [52]. Protein homology analysis indicated that LanB-like proteins are widespread in bacteria [52], so this unusual use of an aminoacyltRNA may actually be common across nature. Interestingly, a subset of these proteins lack the elimination domain and are ...
two types of titin interactions lead to an asymmetrical sorting of actinin
... some fragments is slightly higher. Lane 1, α-actinin 2A (mol. wt 104 kDa); lane 2, R1–C (74 kDa); lane 3, R1–R4 (57 kDa); lane 4, R2–R3 (30 kDa); lane 5, titin zr4–Z4 (44 kDa); lane 6, Zq–Z4 (33 kDa); lane 7, zr2–zr3 (11 kDa). M: marker lane, sizes in kDa. ...
... some fragments is slightly higher. Lane 1, α-actinin 2A (mol. wt 104 kDa); lane 2, R1–C (74 kDa); lane 3, R1–R4 (57 kDa); lane 4, R2–R3 (30 kDa); lane 5, titin zr4–Z4 (44 kDa); lane 6, Zq–Z4 (33 kDa); lane 7, zr2–zr3 (11 kDa). M: marker lane, sizes in kDa. ...
History of the Glycosaminoglycan Symposia in the Scientific Context
... Molecular biology of GAGs with reference to gene transcription US FDA approval of the generic version of enoxaparin Oral anti-Xa and anti-IIa drugs Chemoenzymatic design of heparins Proteoglycans and GAGs in health and disease In depth topics on GAGs as anticancer and anti-inflammatory agents • Adv ...
... Molecular biology of GAGs with reference to gene transcription US FDA approval of the generic version of enoxaparin Oral anti-Xa and anti-IIa drugs Chemoenzymatic design of heparins Proteoglycans and GAGs in health and disease In depth topics on GAGs as anticancer and anti-inflammatory agents • Adv ...
Phylogenetic Classification of Protozoa Based on the
... the linker in P. falciparum is 89 amino acids. The linker in ChDHFR-TS plays an important role in the structure of the enzyme. Compared with the structure of DHFR-TS from PfDHFR-TS (8) in which major portions of the linker domain are not built into the model, the entire linker domain of ChDHFR-TS is ...
... the linker in P. falciparum is 89 amino acids. The linker in ChDHFR-TS plays an important role in the structure of the enzyme. Compared with the structure of DHFR-TS from PfDHFR-TS (8) in which major portions of the linker domain are not built into the model, the entire linker domain of ChDHFR-TS is ...
The Role of the N-Terminal Domains of Bacterial Initiator DnaA in
... among organisms, with greater differences occurring among more unrelated taxonomic groups [3,4]. It is assumed that the molecular mechanism of replication initiation and its control are simplest in bacteria and most complex in Eukaryota. Indeed, the composition of the initiation complex in bacteria ...
... among organisms, with greater differences occurring among more unrelated taxonomic groups [3,4]. It is assumed that the molecular mechanism of replication initiation and its control are simplest in bacteria and most complex in Eukaryota. Indeed, the composition of the initiation complex in bacteria ...
Phylogenetic Classification of Protozoa Based on the Structure of
... the linker in P. falciparum is 89 amino acids. The linker in ChDHFR-TS plays an important role in the structure of the enzyme. Compared with the structure of DHFR-TS from PfDHFR-TS (8) in which major portions of the linker domain are not built into the model, the entire linker domain of ChDHFR-TS is ...
... the linker in P. falciparum is 89 amino acids. The linker in ChDHFR-TS plays an important role in the structure of the enzyme. Compared with the structure of DHFR-TS from PfDHFR-TS (8) in which major portions of the linker domain are not built into the model, the entire linker domain of ChDHFR-TS is ...
BAG1, a negative regulator of Hsp70 chaperone activity, uncouples
... was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addition of ATP (Figure 2, lanes 4–9). In striking contra ...
... was incubated with either Hsp70, or Hsp70 and BAG-1 in the presence or absence of ATP. In the absence of ATP, Hsp70 forms a stable complex with [125I]RCMLA, as detected in the native gel assay (Figure 2, lane 2), that rapidly dissociates upon addition of ATP (Figure 2, lanes 4–9). In striking contra ...
Lipoprotein structure
... phosphatidylcholines and unesterified cholesterol to form lyso PC and the cholesteryl ester products. In this reaction, LCAT uses preferentially PC species with 1 8 : 2 or 18 : 1 fatty acids in the sn-2 position, thus enriching cholesteryl esters in these fatty acids, In contrast, PC containing 1 8 ...
... phosphatidylcholines and unesterified cholesterol to form lyso PC and the cholesteryl ester products. In this reaction, LCAT uses preferentially PC species with 1 8 : 2 or 18 : 1 fatty acids in the sn-2 position, thus enriching cholesteryl esters in these fatty acids, In contrast, PC containing 1 8 ...
ldentification of Surface-Exposed Domains on the Reducing Side of
... complexes with 20 ng Glu-C/mg Chl (Fig. IB). To determine their relative association with the PSI core, the proteasetreated PSI complexes were filtered using the Centricon-100, and retentates were analyzed by Tricine-urea-SDS-PAGE. The El and EII cleavage products of PsaE were more tightly associate ...
... complexes with 20 ng Glu-C/mg Chl (Fig. IB). To determine their relative association with the PSI core, the proteasetreated PSI complexes were filtered using the Centricon-100, and retentates were analyzed by Tricine-urea-SDS-PAGE. The El and EII cleavage products of PsaE were more tightly associate ...
Structure-Function Analysis of SH3 Domains: SH3
... Src SH3 and that the T98D substitution in Abl SH3 can modify the binding specificity of Abl SH3. Tyrosine phosphorylation of SH3-binding proteins. We previously showed that Src SH3 specifically interacted with several tyrosine-phosphorylated proteins from SRD 3T3 cells. Figure 3 shows an antiphospho ...
... Src SH3 and that the T98D substitution in Abl SH3 can modify the binding specificity of Abl SH3. Tyrosine phosphorylation of SH3-binding proteins. We previously showed that Src SH3 specifically interacted with several tyrosine-phosphorylated proteins from SRD 3T3 cells. Figure 3 shows an antiphospho ...
Basic principles of multidimensional NMR in solution General
... Protein structures NMR-spectra of proteins Sequence specific assignment Protein structure determination Ligand-screening Basic principles of multidimensional NMR spectrocopy ...
... Protein structures NMR-spectra of proteins Sequence specific assignment Protein structure determination Ligand-screening Basic principles of multidimensional NMR spectrocopy ...
Acyl-CoA oxidase is imported as a heteropentameric, cofactor
... not assembly in the cytosol, of the Aox complex is abolished by the pex5KO mutation. (A) Equal portions (0.1% of the total volume) of the indicated subcellular fractions isolated from YPBOgrown wild-type (E122), pex5KO, and pex20KO cells were resolved by SDSPAGE and analyzed by immunoblotting with a ...
... not assembly in the cytosol, of the Aox complex is abolished by the pex5KO mutation. (A) Equal portions (0.1% of the total volume) of the indicated subcellular fractions isolated from YPBOgrown wild-type (E122), pex5KO, and pex20KO cells were resolved by SDSPAGE and analyzed by immunoblotting with a ...
Control of Pre-mRNA Splicing by the General Splicing Factors
... [11,12]. Our current understanding of the determinants of exon identity and intron splicing is limited. The well-defined consensus sequences at the 59 and 39 splice sites do not contain sufficient information to accurately identify bona fide splice sites [13], suggesting that additional sequence fea ...
... [11,12]. Our current understanding of the determinants of exon identity and intron splicing is limited. The well-defined consensus sequences at the 59 and 39 splice sites do not contain sufficient information to accurately identify bona fide splice sites [13], suggesting that additional sequence fea ...
The mechanism of Stx2 enrichment in outer membrane vesicles of
... OMVs are dynamic structures. OMVs produced by bacteria vary in size, density, protein and lipid content, and production rate. Since the production of OMVs is a way of interacting with the extracellular milieu, this variability allows a bacterium to respond to changes in its environment. In Neisseria ...
... OMVs are dynamic structures. OMVs produced by bacteria vary in size, density, protein and lipid content, and production rate. Since the production of OMVs is a way of interacting with the extracellular milieu, this variability allows a bacterium to respond to changes in its environment. In Neisseria ...
Ribosomal Stalk Protein L12: Structure, Function and
... factors during various steps of translation. The P-site is located next to the A-site on the ribosome. During initiation of protein synthesis, initiator tRNA (fMet-tRNAfMet) binds directly to the P-site on the 30S subunit. Other than that, peptidyl tRNAs occupy the P-site after translocation until t ...
... factors during various steps of translation. The P-site is located next to the A-site on the ribosome. During initiation of protein synthesis, initiator tRNA (fMet-tRNAfMet) binds directly to the P-site on the 30S subunit. Other than that, peptidyl tRNAs occupy the P-site after translocation until t ...
Roles of Arabidopsis PARC6 in Coordination of
... Membrane Occupation and Recognition Nexus Domain of ARC3 ...
... Membrane Occupation and Recognition Nexus Domain of ARC3 ...
FUNCTIONAL CONSEQUENCES OF THE DIRECT PHYSICAL CBP/p300
... immortal cells from primary bone marrow cultures (13,14). ...
... immortal cells from primary bone marrow cultures (13,14). ...
Antimicrobial peptides in crustaceans
... single AMP family. In terms of structure, crustacean AMPs can be primarily defined as small, cationic, amphipathic molecules that are encoded by a single gene, as commonly described in other organisms. However, more recently, this definition was expanded to include less common AMPs, such as anionic ...
... single AMP family. In terms of structure, crustacean AMPs can be primarily defined as small, cationic, amphipathic molecules that are encoded by a single gene, as commonly described in other organisms. However, more recently, this definition was expanded to include less common AMPs, such as anionic ...
Forced Expression of Dystrophin Deletion Constructs Reveals
... (Fig. 3). These mosaic-expressing mice, however, were able to be analyzed for proper localization of the members of the D A P complex. To establish that formation of the dystroglycan complex is independent of uniform dystrophin expression, we analyzed the pattern of [3-dystroglycan localization in a ...
... (Fig. 3). These mosaic-expressing mice, however, were able to be analyzed for proper localization of the members of the D A P complex. To establish that formation of the dystroglycan complex is independent of uniform dystrophin expression, we analyzed the pattern of [3-dystroglycan localization in a ...
Intragenic Revertants of Yeast Invertase Variants with Secretion-Defective Leader Sequences.
... the secretory pathway (C. A. Kaiser, Ph.D. thesis, Massachusetts Institute of Technology, Cambridge, 1987). Thus, these amino-terminal residues constitute a signal sequence for secretion. They are necessary for invertase secretion, since the constitutive form of invertase remains in the cytoplasm, a ...
... the secretory pathway (C. A. Kaiser, Ph.D. thesis, Massachusetts Institute of Technology, Cambridge, 1987). Thus, these amino-terminal residues constitute a signal sequence for secretion. They are necessary for invertase secretion, since the constitutive form of invertase remains in the cytoplasm, a ...
Organization of Physical Interactomes as
... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
... complex schemas are organized in terms of their lower-order constituents. The uncovered schemas span a wide range of cellular activities, with many signaling and transport related higher-order schemas. We establish the functional importance of the schemas by showing that they correspond to functiona ...
LCAT activation by apoA-I in HDL3 exposed to HOCl or MPO
... MS/MS spectrum (unique peptides) Analysis Quantification Dual Statistical Criteria G-test and t-test ...
... MS/MS spectrum (unique peptides) Analysis Quantification Dual Statistical Criteria G-test and t-test ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.