The Platform Protein Is Essential for Type IV Pilus
... both the motor and alignment subcomplexes in the T4P and T2S systems have been suggested to transduce the conformational changes of the ATPases (26, 27), making it challenging to propose a unifying model of fiber assembly and function. In the P. aeruginosa T4aP system, the platform protein PilC was ...
... both the motor and alignment subcomplexes in the T4P and T2S systems have been suggested to transduce the conformational changes of the ATPases (26, 27), making it challenging to propose a unifying model of fiber assembly and function. In the P. aeruginosa T4aP system, the platform protein PilC was ...
Partial Purifiaction of β-1,3-glucan synthases and
... and anti-HIV activities. Due to these specific immune-potentiating activities, paramylon is novel in terms of both structure as well as functional activity. In terms of biotechnological application, paramylon is greatly favoured as it is synthesized as an insoluble membrane bound granule in the cyto ...
... and anti-HIV activities. Due to these specific immune-potentiating activities, paramylon is novel in terms of both structure as well as functional activity. In terms of biotechnological application, paramylon is greatly favoured as it is synthesized as an insoluble membrane bound granule in the cyto ...
Repeat motifs of tau bind to the insides of microtubules in the
... three tubulin dimers (~3.3 mM 3R-tau to 10 mM tubulin dimers) (Figure 2C). At higher ratios, the gradient of the curve is less steep, indicating weaker binding (~10±6 M). Strong binding of 4R-tau persisted as far as one tau molecule per two to three tubulin dimers (~4 mM 4R-tau to 10 mM tubulin dime ...
... three tubulin dimers (~3.3 mM 3R-tau to 10 mM tubulin dimers) (Figure 2C). At higher ratios, the gradient of the curve is less steep, indicating weaker binding (~10±6 M). Strong binding of 4R-tau persisted as far as one tau molecule per two to three tubulin dimers (~4 mM 4R-tau to 10 mM tubulin dime ...
Schubert, C. M., R. Lin, C. J. de Vries, R. H. A.
... sperm entry appears to define the posterior pole of the C. elegans embryo. After fertilization, the proteins PAR-1 and PAR-2 localize to the posterior cortex of the embryo (Guo and Kemphues, 1995; Boyd et al., 1996) and PAR-3, PKC-3, and PAR-6 localize to the anterior cortex (Etemad-Moghadam et al., ...
... sperm entry appears to define the posterior pole of the C. elegans embryo. After fertilization, the proteins PAR-1 and PAR-2 localize to the posterior cortex of the embryo (Guo and Kemphues, 1995; Boyd et al., 1996) and PAR-3, PKC-3, and PAR-6 localize to the anterior cortex (Etemad-Moghadam et al., ...
Golgins and GTPases, giving identity and structure to the Golgi
... but autoantibodies to them may well arise as a result of the breakdown of the Golgi during apoptosis and necrosis leading to the generation of multiple antigenic fragments from the repetitive coiled-coil domains [13]. More recently, several additional examples of coiled-coil proteins localizing to t ...
... but autoantibodies to them may well arise as a result of the breakdown of the Golgi during apoptosis and necrosis leading to the generation of multiple antigenic fragments from the repetitive coiled-coil domains [13]. More recently, several additional examples of coiled-coil proteins localizing to t ...
Salmonella pathogenicity island 1 (SPI-1) type III
... the bacterial cell (Kim et al., 2007). However, in silico comparison of the N-terminal signal regions of known effectors has not led to the identification of any conserved sequences, implying that features such as amphipathicity or secondary structure serve as recognition motifs (Lloyd et al., 2001, ...
... the bacterial cell (Kim et al., 2007). However, in silico comparison of the N-terminal signal regions of known effectors has not led to the identification of any conserved sequences, implying that features such as amphipathicity or secondary structure serve as recognition motifs (Lloyd et al., 2001, ...
Identification of a C-terminal Poly(A)-binding Protein (PABP)
... tract mRNA family (23), which includes mRNAs encoding components of the protein synthesis machinery. Translation of these mRNAs is linked to the growth status of the cell, and this regulation strictly requires a short sequence of polypyrimidines at their 5⬘ end (reviewed in Ref. 24). Control of PABP ...
... tract mRNA family (23), which includes mRNAs encoding components of the protein synthesis machinery. Translation of these mRNAs is linked to the growth status of the cell, and this regulation strictly requires a short sequence of polypyrimidines at their 5⬘ end (reviewed in Ref. 24). Control of PABP ...
In Vitro Assays Demonstrate That Pollen Tube Organelles Use
... characterized in the pollen tube. A pollen kinesin homolog (Tiezzi et al., 1992) and two dynein-related polypeptides (Moscatelli et al., 1995) have been identified in the vegetative cytoplasm of pollen tubes and localized in association with unclassified membrane structures (Cai et al., 1993; Moscat ...
... characterized in the pollen tube. A pollen kinesin homolog (Tiezzi et al., 1992) and two dynein-related polypeptides (Moscatelli et al., 1995) have been identified in the vegetative cytoplasm of pollen tubes and localized in association with unclassified membrane structures (Cai et al., 1993; Moscat ...
Identification of proteins localized to the contractile vacuole of
... belong to families of small and membrane-associated proteins that are distributed on the cytoplasmic surfaces of all membranes of the secretory pathway and are involved in membrane trafficking (Mayer et al. 1996; Weber et al. 1998). The membrane trafficking of transport vesicles delivers proteins, h ...
... belong to families of small and membrane-associated proteins that are distributed on the cytoplasmic surfaces of all membranes of the secretory pathway and are involved in membrane trafficking (Mayer et al. 1996; Weber et al. 1998). The membrane trafficking of transport vesicles delivers proteins, h ...
Capping protein: new insights into mechanism
... stability and global structure of the CP truncation and point mutants appeared unchanged [22,23], suggesting that the amino acids deleted and substituted are indeed functionally important for the capping activity of CP. In addition, the C-terminal 28 or 34 amino acids of the chicken CP b1 subunit (F ...
... stability and global structure of the CP truncation and point mutants appeared unchanged [22,23], suggesting that the amino acids deleted and substituted are indeed functionally important for the capping activity of CP. In addition, the C-terminal 28 or 34 amino acids of the chicken CP b1 subunit (F ...
The Plant Cell - Department of Biology
... binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion protein is predicted to produce a polypeptide of 33 kD. The expressed proteins were isolated and a polypeptide o ...
... binding properties using a bacterially expressed protein. The full-length KIC cDNA sequence was inserted into pET32a vector, which expresses the KIC protein as an S-tag fusion. The fusion protein is predicted to produce a polypeptide of 33 kD. The expressed proteins were isolated and a polypeptide o ...
Endoplasmic Reticulum-Associated Degradation of Ricin A Chain
... Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom (A.D.C., L.F., J.M.L., L.M.R.); and Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy (A.C.) ...
... Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, United Kingdom (A.D.C., L.F., J.M.L., L.M.R.); and Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, 20133 Milan, Italy (A.C.) ...
Article (Published version)
... the CNS homeodomain code, the latter being required for specification of different neuronal subtypes (Briscoe et al., 2000). However, the variable results make a unifying interpretation difficult. Similarly, their function in primary neurogenesis is not completely clear. Pan-Gli dominant-negative as ...
... the CNS homeodomain code, the latter being required for specification of different neuronal subtypes (Briscoe et al., 2000). However, the variable results make a unifying interpretation difficult. Similarly, their function in primary neurogenesis is not completely clear. Pan-Gli dominant-negative as ...
The contribution of the Trp/Met/Phe residues to physical interactions
... transcriptional machinery. The interacting proteins include the TATA box binding protein and the associated factor TFIID [22]. Even though there is no structural information about the contribution of Phe19 and Trp23, their involvement can be inferred from their critical roles in p53 transactivation ...
... transcriptional machinery. The interacting proteins include the TATA box binding protein and the associated factor TFIID [22]. Even though there is no structural information about the contribution of Phe19 and Trp23, their involvement can be inferred from their critical roles in p53 transactivation ...
curriculum vitae- michael hippler
... efficiency of cyclic electron transfer (CEF) under iron-deprivation is diminished. Furthermore, pgrl1-kd cells exhibit iron-deficiency symptoms at higher Fe concentrations than does the wild-type, although the cells are not more depleted in cellular iron relative to the wild-type as measured by mas ...
... efficiency of cyclic electron transfer (CEF) under iron-deprivation is diminished. Furthermore, pgrl1-kd cells exhibit iron-deficiency symptoms at higher Fe concentrations than does the wild-type, although the cells are not more depleted in cellular iron relative to the wild-type as measured by mas ...
The Foamy Virus Gag Proteins: What Makes Them Different?
... Several studies show that the N terminus of FV Gag is essential for the interaction of Gag with Env [18,24-26]. Thereby, the structural integrity of a predicted coiled-coil motif (CC1) at aa 4–19 seems to be important for the interaction [18]. Other retroviral Gag proteins, like HIV-1 Gag, contain s ...
... Several studies show that the N terminus of FV Gag is essential for the interaction of Gag with Env [18,24-26]. Thereby, the structural integrity of a predicted coiled-coil motif (CC1) at aa 4–19 seems to be important for the interaction [18]. Other retroviral Gag proteins, like HIV-1 Gag, contain s ...
Ph.D. Thesis Azar Shahpiri
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
... acid (GA) signal by synthesizing hydrolytic enzymes that are released to the endosperm, before undergoing cell death. The barley (Hordeum vulgare) aleurone layer can be separated from the other seed tissues and maintained in culture, allowing the study of GA, abscisic acid (ABA) and other signals in ...
pdf file - John Innes Centre
... ability to interact with the beads. This result was also confirmed using purified GlnK (data not shown). Co-purification of AmtB6H and GlnK from GT1000(pJT6) extracts was also demonstrated using Ni2⫹ affinity chromatography (data not shown). These results confirm that AmtB interacts with GlnK in viv ...
... ability to interact with the beads. This result was also confirmed using purified GlnK (data not shown). Co-purification of AmtB6H and GlnK from GT1000(pJT6) extracts was also demonstrated using Ni2⫹ affinity chromatography (data not shown). These results confirm that AmtB interacts with GlnK in viv ...
Actin Filaments Play a Critical Role in Vacuolar
... transformed with wild-type actin2 showed numerous actin filaments in a staining pattern that was nearly identical to that of protoplasts expressing GFP:talin alone (data not shown). Thus, overexpression of wild-type actin2 does not affect morphology of actin filaments. By contrast, overexpression of ...
... transformed with wild-type actin2 showed numerous actin filaments in a staining pattern that was nearly identical to that of protoplasts expressing GFP:talin alone (data not shown). Thus, overexpression of wild-type actin2 does not affect morphology of actin filaments. By contrast, overexpression of ...
Arabidopsis RTM1 and RTM2 Genes Function in Phloem to Restrict
... Similar results were obtained using anti-RTM1 produced in each of four chickens (data not shown). The lack of detection of RTM1 in various tissues, ecotypes, and rtm1 mutant lines may be due to several factors. For instance, RTM1 may be present but in concentrations below the level of detection usin ...
... Similar results were obtained using anti-RTM1 produced in each of four chickens (data not shown). The lack of detection of RTM1 in various tissues, ecotypes, and rtm1 mutant lines may be due to several factors. For instance, RTM1 may be present but in concentrations below the level of detection usin ...
S C T
... sphingolipids and cholesterol, where glycerophospholipids are the main lipid constituent of most biomembranes. Lipids are amphiphilic molecules, i.e. they contain both hydrophobic and hydrophilic regions. In glycerophospholipids and sphingolipids, the hydrophobic part is composed of the fatty acid ( ...
... sphingolipids and cholesterol, where glycerophospholipids are the main lipid constituent of most biomembranes. Lipids are amphiphilic molecules, i.e. they contain both hydrophobic and hydrophilic regions. In glycerophospholipids and sphingolipids, the hydrophobic part is composed of the fatty acid ( ...
Post-transcriptional regulation of auxin transport proteins: cellular
... Post-transcriptional regulation of auxin transport proteins | 1095 mutational analysis indicates that PIN3 functions in tropic responses, activation of its transport activity near sites of illumination could be expected to accelerate auxin movement out of tissues on the illuminated (non-bending sid ...
... Post-transcriptional regulation of auxin transport proteins | 1095 mutational analysis indicates that PIN3 functions in tropic responses, activation of its transport activity near sites of illumination could be expected to accelerate auxin movement out of tissues on the illuminated (non-bending sid ...
Pericentriolar material structure and dynamics
... but rather a dynamic collection of protein complexes and nucleic acids that constitute the organelle’s interior and determine its boundary. How is the complex biochemical machinery necessary for the myriad centrosome functions concentrated and maintained in the PCM? Recent advances in proteomics and ...
... but rather a dynamic collection of protein complexes and nucleic acids that constitute the organelle’s interior and determine its boundary. How is the complex biochemical machinery necessary for the myriad centrosome functions concentrated and maintained in the PCM? Recent advances in proteomics and ...
Reconstitution of an Allophycocyanin Trimer Complex Containing
... gy tran sfer from the rods th ro u g h the core to the photosystem s. Specific interactio n s w ith the bilip roteins are necessary to explain the functions o f Lcm as a stru ctu ral protein o f the core, as the a n ch o r protein to the thylakoid m em brane, as a link er m odifying the spectral ...
... gy tran sfer from the rods th ro u g h the core to the photosystem s. Specific interactio n s w ith the bilip roteins are necessary to explain the functions o f Lcm as a stru ctu ral protein o f the core, as the a n ch o r protein to the thylakoid m em brane, as a link er m odifying the spectral ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.