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... full-length CD34, having a relatively short intracellular region of 73 amino acids. An alternatively spliced form with a shorter intracellular tail is expressed at lower abundance. Full-length CD34 is highly phosphorylated by protein kinase C (PKC).5 CD34 itself does not have intrinsic kinase activi ...
... full-length CD34, having a relatively short intracellular region of 73 amino acids. An alternatively spliced form with a shorter intracellular tail is expressed at lower abundance. Full-length CD34 is highly phosphorylated by protein kinase C (PKC).5 CD34 itself does not have intrinsic kinase activi ...
Keyhole Limpet Hemocyanin ( KLH )
... precipitation. A slightly acidic pH (~pH 6.5) and the concentration of MgSO4 (≥10 mM MgSO4 is recommended for most procedures) are essential for facilitating and maintaining solubility. While aggregation may not affect KLH immunogenicity by itself, it affects the real available protein quantity. Hen ...
... precipitation. A slightly acidic pH (~pH 6.5) and the concentration of MgSO4 (≥10 mM MgSO4 is recommended for most procedures) are essential for facilitating and maintaining solubility. While aggregation may not affect KLH immunogenicity by itself, it affects the real available protein quantity. Hen ...
Mitochondrial translation factors of Trypanosoma brucei: elongation
... mentation experiments shows that this subdomain is essential for EF-Tu function, but that it can be replaced by a similar sequence found in eukaryotic EF-1a, the cytosolic counterpart of EF-Tu. A recent cryo-electron microscopy study revealed that trypanosomatid mitochondrial ribosomes have a unique ...
... mentation experiments shows that this subdomain is essential for EF-Tu function, but that it can be replaced by a similar sequence found in eukaryotic EF-1a, the cytosolic counterpart of EF-Tu. A recent cryo-electron microscopy study revealed that trypanosomatid mitochondrial ribosomes have a unique ...
... Polar transport–dependent local accumulation of auxin provides positional cues for multiple plant patterning processes. This directional auxin flow depends on the polar subcellular localization of the PIN auxin efflux regulators. Overexpression of the PINOID protein kinase induces a basal-toapical s ...
Divergent Functional Properties of the Ribosome
... several Hsp70s have been solved (Flaherty et al., 1990; Harrison et al., 1997; Sriram et al., 1997). These structures resemble that of actin and hexokinase, consisting of two lobes with a deep cleft between them in which nucleotide binds. More recently, the structure of the 18-kDa peptide-binding do ...
... several Hsp70s have been solved (Flaherty et al., 1990; Harrison et al., 1997; Sriram et al., 1997). These structures resemble that of actin and hexokinase, consisting of two lobes with a deep cleft between them in which nucleotide binds. More recently, the structure of the 18-kDa peptide-binding do ...
Rubisco Synthesis, Assembly, Mechanism, and Regulation
... dimeric form of the enzyme have survived the increased levels of oxygen in the atmosphere because they are able to switch to a more efficient form I enzyme, particularly under conditions in which low CC>2 prevails. Form I Rubisco is composed of two different-sized subunits. In addition to eight L-su ...
... dimeric form of the enzyme have survived the increased levels of oxygen in the atmosphere because they are able to switch to a more efficient form I enzyme, particularly under conditions in which low CC>2 prevails. Form I Rubisco is composed of two different-sized subunits. In addition to eight L-su ...
Designing logical codon reassignment
... backbone, may act as reporters of change in chemical environment occurring at the amino acid residue level. Examples of such spectroscopically-active amino acid side chains include uorophores for uorescent spectroscopy, spin labels for NMR and EPR, heavy atoms for X-ray crystallography or amino ac ...
... backbone, may act as reporters of change in chemical environment occurring at the amino acid residue level. Examples of such spectroscopically-active amino acid side chains include uorophores for uorescent spectroscopy, spin labels for NMR and EPR, heavy atoms for X-ray crystallography or amino ac ...
The AP-3 adaptor complex is required for vacuolar function
... BFA compartments that consist of aggregated TGN/EEs and recycling endosomes with Golgi apparatus (GA) at their periphery [17, 24-26]. A 1 h treatment with 50 µM BFA, combined with PM labeling by 4 µM endocytic tracer FM4-64 [27] led to a rapid internalization of PMlocalized PIN1-GFP and its colocali ...
... BFA compartments that consist of aggregated TGN/EEs and recycling endosomes with Golgi apparatus (GA) at their periphery [17, 24-26]. A 1 h treatment with 50 µM BFA, combined with PM labeling by 4 µM endocytic tracer FM4-64 [27] led to a rapid internalization of PMlocalized PIN1-GFP and its colocali ...
Gene Section HDAC3 (histone deacetylase 3) Atlas of Genetics and Cytogenetics
... specific promoters. Thus, HDAC3 is able to regulate osteoblast differentiation and bone formation via its association with the osteoblast master protein, Runx2, and the inhibition of the trans-activity of Runx2. Likewise, in hematopoietic stem cells, HDAC3, but not other class I HDACs, directly asso ...
... specific promoters. Thus, HDAC3 is able to regulate osteoblast differentiation and bone formation via its association with the osteoblast master protein, Runx2, and the inhibition of the trans-activity of Runx2. Likewise, in hematopoietic stem cells, HDAC3, but not other class I HDACs, directly asso ...
Structure and Function Relationships between ATPase Family, AAA
... 1.1 Translesion DNA Synthesis Faithful replication of a cell’s genetic material during replication is essential in order to pass genetic material from one generation to the next; however, DNA-damaging agents constantly injure the genome. High fidelity replication of the cell’s genetic material is no ...
... 1.1 Translesion DNA Synthesis Faithful replication of a cell’s genetic material during replication is essential in order to pass genetic material from one generation to the next; however, DNA-damaging agents constantly injure the genome. High fidelity replication of the cell’s genetic material is no ...
Identification of Saccharomyces cerevisiae proteins that bind to
... The following individuals and institutions are hereby acknowledged and thanked for their contribution to this project. Dr Gabre Kemp, thank you for your support and sacrifice in the completion of this project. Your willingness to help and guide me along the way is greatly appreciated. Thank you for ...
... The following individuals and institutions are hereby acknowledged and thanked for their contribution to this project. Dr Gabre Kemp, thank you for your support and sacrifice in the completion of this project. Your willingness to help and guide me along the way is greatly appreciated. Thank you for ...
Dissociation of a ll0-kD Peripheral Membrane Protein from the Golgi
... are sorted en route to their final destination inside or outside of the cell. The striking morphology of this organdie, consisting of ordered stacks of cisternae, reflects a spatially differentiated structure that is believed to correlate with distinct Golgi functions (lhdade, 1975; Farquhar, 1985; ...
... are sorted en route to their final destination inside or outside of the cell. The striking morphology of this organdie, consisting of ordered stacks of cisternae, reflects a spatially differentiated structure that is believed to correlate with distinct Golgi functions (lhdade, 1975; Farquhar, 1985; ...
HIBERNATING BEARS, ANTIBIOTICS AND THE EVOLVING RIBOSOME
... When more suitable synchrotron facilities became available, and several crystal forms were grown (Figure 4), the radiation sensitivity of the ribosomal crystals caused extremely fast crystal decay. Hence, pioneering data collection at cryo temperature became crucial (Hope et al., 1989), and once est ...
... When more suitable synchrotron facilities became available, and several crystal forms were grown (Figure 4), the radiation sensitivity of the ribosomal crystals caused extremely fast crystal decay. Hence, pioneering data collection at cryo temperature became crucial (Hope et al., 1989), and once est ...
The green fluorescent protein: discovery
... By expressing full-length GFP-tagged proteins from their endogenous chromosomal locations at natural levels, it has become feasible to monitor the intracellular location and concentration spectrum of the whole proteasome of different organisms (Ghaemmaghami et al., 2003). Another window for detailed ...
... By expressing full-length GFP-tagged proteins from their endogenous chromosomal locations at natural levels, it has become feasible to monitor the intracellular location and concentration spectrum of the whole proteasome of different organisms (Ghaemmaghami et al., 2003). Another window for detailed ...
Organic_2_6.1ed_2012_10th_module_biochem_and_polymers
... With respect to carbohydrates, know: The approximate empirical formula characteristic of members of the class (CH2O). The functional groups present in carbohydrate molecules: alcohols (usually several are present); aldehydes and ketones; hemiacetals and acetals Carbohydrates belonging to these class ...
... With respect to carbohydrates, know: The approximate empirical formula characteristic of members of the class (CH2O). The functional groups present in carbohydrate molecules: alcohols (usually several are present); aldehydes and ketones; hemiacetals and acetals Carbohydrates belonging to these class ...
Incorporating key position and amino acid residue features to
... prediction of ubiquitylation sites is a potentially useful alternative strategy for whole proteome annotation. Because of various limitations, current ubiquitylation site prediction tools were not well designed to comprehensively assess proteomes. Results: We present a novel tool known as UbiProber, ...
... prediction of ubiquitylation sites is a potentially useful alternative strategy for whole proteome annotation. Because of various limitations, current ubiquitylation site prediction tools were not well designed to comprehensively assess proteomes. Results: We present a novel tool known as UbiProber, ...
Functional Characterisation of Plant
... contain a conserved PRAT domain and sterile alpha motif (SAM) domain. The latter of which is known to be involved in protein-protein interaction and RNA binding. Utilising T-DNA insertional knockout lines, the function of At5g24650 was investigated and found to have a potential role in the import of ...
... contain a conserved PRAT domain and sterile alpha motif (SAM) domain. The latter of which is known to be involved in protein-protein interaction and RNA binding. Utilising T-DNA insertional knockout lines, the function of At5g24650 was investigated and found to have a potential role in the import of ...
PDF - Oxford Academic - Oxford University Press
... SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of these methods, called DIVERGE (17) takes into account the di ...
... SDPpred does not require information about the 3D protein structure, which instead can be used at the post-processing step to validate the predictions. There are also other approaches for comparative analysis of protein subfamilies. One of these methods, called DIVERGE (17) takes into account the di ...
PDF
... Sm proteins constitute the common core of spliceosomal small nuclear ribonucleoproteins. Although Sm proteins are known to be methylated at specific arginine residues within the C-terminal arginine-glycine dipeptide (RG) repeats, the biological relevance of these modifications remains unknown. In th ...
... Sm proteins constitute the common core of spliceosomal small nuclear ribonucleoproteins. Although Sm proteins are known to be methylated at specific arginine residues within the C-terminal arginine-glycine dipeptide (RG) repeats, the biological relevance of these modifications remains unknown. In th ...
Using Mascot to characterise protein modifications
... This slide shows three peptides with the same parent mass, but they differ in the PTM site(s). There are 4 Lysines that can be modified, there is the amino terminus and a Ser and an Arg residue. The possible combination of PTM sites is large. The bottom spectrum demonstrates how four different PTMs ...
... This slide shows three peptides with the same parent mass, but they differ in the PTM site(s). There are 4 Lysines that can be modified, there is the amino terminus and a Ser and an Arg residue. The possible combination of PTM sites is large. The bottom spectrum demonstrates how four different PTMs ...
Lens Major Intrinsic Protein (MIP)
... efficiency. In five experiments using the probe dilution assay, no change in the NBD emission was observed, although the turbidity assay (see below) revealed that aggregates were forming. Thus, the interaction between junctional proteins and PS membranes is adhesive only and does not destabilize mem ...
... efficiency. In five experiments using the probe dilution assay, no change in the NBD emission was observed, although the turbidity assay (see below) revealed that aggregates were forming. Thus, the interaction between junctional proteins and PS membranes is adhesive only and does not destabilize mem ...
The LIR motif – crucial for selective autophagy
... Fig. 3. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A) Surface representation of LC3B bound to the p62-LIR peptide (top left), yeast Atg8 bound to the Atg19-LIR peptide (top right), GABARAP-L1 bound to the NBR1-LIR peptide (bottom left) and LC3C bound to the NDP52-LIR ...
... Fig. 3. LIR motif consensus and structural determinants of LIR–ATG8 interactions. (A) Surface representation of LC3B bound to the p62-LIR peptide (top left), yeast Atg8 bound to the Atg19-LIR peptide (top right), GABARAP-L1 bound to the NBR1-LIR peptide (bottom left) and LC3C bound to the NDP52-LIR ...
Structural Analyses of Zinc Finger Domains for Specific Interactions
... abilities to target nucleic acids as a part of nucleases. Most zinc finger proteins have more than one domain, and many biophysical reports proved that one zinc finger domain is not enough to obtain specific bindings [60, 76], although one GAGA binding domain from Drosophila has a significantly high ...
... abilities to target nucleic acids as a part of nucleases. Most zinc finger proteins have more than one domain, and many biophysical reports proved that one zinc finger domain is not enough to obtain specific bindings [60, 76], although one GAGA binding domain from Drosophila has a significantly high ...
A Comprehensive Mutational Analysis of the
... reveal that two arginine (R)– or lysine (K)–enriched short motifs (i.e., R/K-R/K-x-R/K) make up the likely core EHM-targeting signals, which, together with the N-terminal transmembrane domain, define a minimal sequence of 60 amino acids that is necessary and sufficient for EHM localization. In additio ...
... reveal that two arginine (R)– or lysine (K)–enriched short motifs (i.e., R/K-R/K-x-R/K) make up the likely core EHM-targeting signals, which, together with the N-terminal transmembrane domain, define a minimal sequence of 60 amino acids that is necessary and sufficient for EHM localization. In additio ...
as PDF
... receptor and TNF-α (11). Interestingly, gAcrp30 and TNF-α, which exert opposing effects on the development of atherosclerosis, have unrelated amino-acid sequences, but display highly similar homo-trimeric three-dimensional structures (figure 2) (13). The signaling receptors used by gAcrp30 and adipo ...
... receptor and TNF-α (11). Interestingly, gAcrp30 and TNF-α, which exert opposing effects on the development of atherosclerosis, have unrelated amino-acid sequences, but display highly similar homo-trimeric three-dimensional structures (figure 2) (13). The signaling receptors used by gAcrp30 and adipo ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.