Intraflagellar transport and the generation of dynamic, structurally
... homeodomain transcription factor, TTX-1, is largely sufficient for specifying the conversion of a rod-type cilium into a highly differentiated AFD-like thermosensory cilium in C. elegans [21,50]. To what extent such factors contribute specifically to ciliary fate determination, as opposed to the gen ...
... homeodomain transcription factor, TTX-1, is largely sufficient for specifying the conversion of a rod-type cilium into a highly differentiated AFD-like thermosensory cilium in C. elegans [21,50]. To what extent such factors contribute specifically to ciliary fate determination, as opposed to the gen ...
BMP binding domains in the extracellular space
... In the case of fibrillar procollagens I, II, III and V, which are the major components of the ECM of skin, bones and cartilage (Scriver et al., 1995), the CR domain is present in the NH2propeptide. It has recently been shown that BMP2 and TGFβ1 can bind to the procollagen IIA CR domain (Zhu et al., ...
... In the case of fibrillar procollagens I, II, III and V, which are the major components of the ECM of skin, bones and cartilage (Scriver et al., 1995), the CR domain is present in the NH2propeptide. It has recently been shown that BMP2 and TGFβ1 can bind to the procollagen IIA CR domain (Zhu et al., ...
Recoding the Genetic Code with Selenocysteine
... fied proteins, there is an urgent need to synthesize proteins incorporation in response to UGA and 95.1 % Sec insertion containing several different, distinct ncAAs. Progress in the for UAC in TrxR (Table S3). last decade allowed site-specific incorporation of one or two It remains unclear why certa ...
... fied proteins, there is an urgent need to synthesize proteins incorporation in response to UGA and 95.1 % Sec insertion containing several different, distinct ncAAs. Progress in the for UAC in TrxR (Table S3). last decade allowed site-specific incorporation of one or two It remains unclear why certa ...
TAFs and the Mediator
... 2. interaction with core-promoter elements supported by genome-wide analysis in yeast ...
... 2. interaction with core-promoter elements supported by genome-wide analysis in yeast ...
Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell
... participate in wall construction as hydrolases or transglycosidases. The second contains nonenzymatic agglutinins, flocculins, or b1,3-glucan cross-connectors (Klis et al. 2006, 2010; Dranginis et al. 2007; Goossens and Willaert ...
... participate in wall construction as hydrolases or transglycosidases. The second contains nonenzymatic agglutinins, flocculins, or b1,3-glucan cross-connectors (Klis et al. 2006, 2010; Dranginis et al. 2007; Goossens and Willaert ...
Effects of macromolecular crowding on protein folding and
... thiols may involve other factors (Cuozzo and Kaiser, 1999). The results confirm that reduced lysozyme is prone to aggregation during its refolding; at an optimal lysozyme concentration of 5 µM, the refolding yields are only ~20– 25%. Allowing for different experimental conditions such as pH and temp ...
... thiols may involve other factors (Cuozzo and Kaiser, 1999). The results confirm that reduced lysozyme is prone to aggregation during its refolding; at an optimal lysozyme concentration of 5 µM, the refolding yields are only ~20– 25%. Allowing for different experimental conditions such as pH and temp ...
Bacterial protein toxins targeting Rho GTPases
... GTPases (e.g. Cdc42 and Rac) [10]. C3 exoenzymes ADPribosylate Rho at Asn41 [11] thereby blocking Rho-mediated signalling. Although the target amino acid residue is located near the e¡ector region of Rho, ADP-ribosylation does not block the Rho/e¡ector interaction [12] nor are major e¡ects on guanin ...
... GTPases (e.g. Cdc42 and Rac) [10]. C3 exoenzymes ADPribosylate Rho at Asn41 [11] thereby blocking Rho-mediated signalling. Although the target amino acid residue is located near the e¡ector region of Rho, ADP-ribosylation does not block the Rho/e¡ector interaction [12] nor are major e¡ects on guanin ...
TSE diagnostics 4
... Atypical scrapie Atypical scrapie was first recorded in Norway (Benestad and others 2003) and reports of other `atypical' TSE phenotypes, including those that are similar to or the same as Nor98 have now been published, from France (Buschmann and others 2004b), Germany (Buschmann and others 2004a ...
... Atypical scrapie Atypical scrapie was first recorded in Norway (Benestad and others 2003) and reports of other `atypical' TSE phenotypes, including those that are similar to or the same as Nor98 have now been published, from France (Buschmann and others 2004b), Germany (Buschmann and others 2004a ...
Identification and analysis of new phloem proteins from
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
... The organelles that can be found in mature SE (mitochondria, plastids, endoplasmic reticulum) appear to be anchored to a membrane system that probably also protects some of the SE proteins from the bulk flow (Ehlers et al., 2000). The ER in SE is modified and is proposed to function as a rail system ...
A Raman spectroscopic study of the interaction between nucleotides
... Raman spectra of the bacteriophage T4 denaturing protein gp32, its complex with the polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA), and (dA),, were recorded and interpreted. According to an analysis of the gp32 spectra with the reference inte ...
... Raman spectra of the bacteriophage T4 denaturing protein gp32, its complex with the polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA), and (dA),, were recorded and interpreted. According to an analysis of the gp32 spectra with the reference inte ...
Supplementary Table 1
... Genes of strain BWG1-7A significantly up- or down-regulated during the four hours time-course of growth in the presence of 100 mM L-serine are listed in table IA and table IB respectively. Genes listed are those whose expression was significantly altered by at least two-fold (Welch’s t-test analysis ...
... Genes of strain BWG1-7A significantly up- or down-regulated during the four hours time-course of growth in the presence of 100 mM L-serine are listed in table IA and table IB respectively. Genes listed are those whose expression was significantly altered by at least two-fold (Welch’s t-test analysis ...
Protein Interaction Technical Handbook
... The natural affinity of binding partners for each other is at the core of in vitro methods widely adopted for both interaction discovery and confirmation. In vitro methods span a broad range of techniques. At one end of the range are those methods that can be performed at the bench with basic labora ...
... The natural affinity of binding partners for each other is at the core of in vitro methods widely adopted for both interaction discovery and confirmation. In vitro methods span a broad range of techniques. At one end of the range are those methods that can be performed at the bench with basic labora ...
The TN5 Transposon - Biochemistry | UW-Madison
... give yet more examples of how gene expression can be modulated. The transposase is a protein that performs multiple complexfunctions. Protein structure/function studies would seek answers to the questions about the organization of the peptide domains that perform these functions and how they interac ...
... give yet more examples of how gene expression can be modulated. The transposase is a protein that performs multiple complexfunctions. Protein structure/function studies would seek answers to the questions about the organization of the peptide domains that perform these functions and how they interac ...
REVIEWS - Unisciel
... ~160 and ~190 kDa that remain tightly bound during purification of titin6. Negative staining also shows the bead-like sub-structure with a periodicity of about 4 nm; this correlates with the multi-domain structure that is inferred from primary sequence. ...
... ~160 and ~190 kDa that remain tightly bound during purification of titin6. Negative staining also shows the bead-like sub-structure with a periodicity of about 4 nm; this correlates with the multi-domain structure that is inferred from primary sequence. ...
DOMAIN-BASED APPROACHES TO
... identified with the topology of the binding core and pore-loop of the eukaryotic iGluR’s. Group 1 has a potassium-like selectivity filter and Group 2 is most closely related to eukaryotic iGluR’s. The relationship among them was investigated in this research. Then, the domain complexity of proteins ...
... identified with the topology of the binding core and pore-loop of the eukaryotic iGluR’s. Group 1 has a potassium-like selectivity filter and Group 2 is most closely related to eukaryotic iGluR’s. The relationship among them was investigated in this research. Then, the domain complexity of proteins ...
Detergents and Surfactants
... When selecting a detergent, the first consideration is usually the ionic form of the hydrophilic group, which is anionic, cationic, zwitterionic, or non-ionic. Anionic and cationic detergents typically modify protein structure to a greater extent than the other two classes. The degree of modificatio ...
... When selecting a detergent, the first consideration is usually the ionic form of the hydrophilic group, which is anionic, cationic, zwitterionic, or non-ionic. Anionic and cationic detergents typically modify protein structure to a greater extent than the other two classes. The degree of modificatio ...
p62/SQSTM1 Binds Directly to Atg8/LC3 to Facilitate Degradation of
... Recently, the term ALIS (aggresome-like induced structures) was used to describe ubiquitin-containing bodies induced in response to various stressors, including amino acid starvation, oxidative stress, and puromycin (17). ALIS refers to DALIS (dendritic cell aggresome-like induced structures) origin ...
... Recently, the term ALIS (aggresome-like induced structures) was used to describe ubiquitin-containing bodies induced in response to various stressors, including amino acid starvation, oxidative stress, and puromycin (17). ALIS refers to DALIS (dendritic cell aggresome-like induced structures) origin ...
Free Full Text ( Final Version , 5mb ) - EUR RePub
... at, 1987). However, ubiquitin has also been found coupled to stable proteins and ubiquitin-conjugating enzymes have been implicated in other basic cellular functions such as DNA repair and cell cycle control (for review see Jentsch et at, 1991 ). Therefore, it is now understood that the marking of p ...
... at, 1987). However, ubiquitin has also been found coupled to stable proteins and ubiquitin-conjugating enzymes have been implicated in other basic cellular functions such as DNA repair and cell cycle control (for review see Jentsch et at, 1991 ). Therefore, it is now understood that the marking of p ...
TAFs and the Mediator
... Basal trx.activity requires: core RNAPII + GTFs Activator-responsive trx. activity requires : core RNAPII + GTFs + Coactivator ...
... Basal trx.activity requires: core RNAPII + GTFs Activator-responsive trx. activity requires : core RNAPII + GTFs + Coactivator ...
Translocation of Structural P Proteins in the Phloem
... of Cucumis sativus scions, whereas Cucurbita maxima PP2 was detected in both sieve elements and companion cells. ...
... of Cucumis sativus scions, whereas Cucurbita maxima PP2 was detected in both sieve elements and companion cells. ...
EMBL-EBI Powerpoint Presentation
... • Homology can be reliably inferred from statistically significant similarity Homology = common 3D structure Homology - NOT common function ...
... • Homology can be reliably inferred from statistically significant similarity Homology = common 3D structure Homology - NOT common function ...
Structure and properties of the outer membranes of Brucella abortus
... properties (see Hydrophobicity). Group 3 Two proteins of 25 and 31 kDa with only 34% of identity are included in group 3 [50] and they are coded for by the omp25 and omp31 genes [5]. Based on algorithmic analyses of the predicted amino acid sequences, none of these two proteins would show the hydrop ...
... properties (see Hydrophobicity). Group 3 Two proteins of 25 and 31 kDa with only 34% of identity are included in group 3 [50] and they are coded for by the omp25 and omp31 genes [5]. Based on algorithmic analyses of the predicted amino acid sequences, none of these two proteins would show the hydrop ...
Glycoxidation of biological macromolecules: A critical
... In diabetes, hyperglycemia induces modification of plasma and tissue proteins by nonenzymatic glycation, a gradual process that culminates with the formation of irreversible AGE. AGE accumulates particularly at sites of atherosclerotic lesions, but the mechanisms whereby AGE contributes to diabetes-i ...
... In diabetes, hyperglycemia induces modification of plasma and tissue proteins by nonenzymatic glycation, a gradual process that culminates with the formation of irreversible AGE. AGE accumulates particularly at sites of atherosclerotic lesions, but the mechanisms whereby AGE contributes to diabetes-i ...
HCM - Cardiology
... • autosomal dominant • usually missense mutations in genes that encode sarcomere proteins • HCM mutant proteins are incorporated into cardiac myofilaments ...
... • autosomal dominant • usually missense mutations in genes that encode sarcomere proteins • HCM mutant proteins are incorporated into cardiac myofilaments ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.