Characterization of Phosphorylation Sites from the Activation Loop
... Reversible protein phosphorylation catalyzed by protein kinases is universally employed by eukaryotes to regulate enzyme activity, protein-protein interactions, subcelluar localization and protein turnover. The catalytic domains of most eukaryotic protein kinases are conserved in their primary seque ...
... Reversible protein phosphorylation catalyzed by protein kinases is universally employed by eukaryotes to regulate enzyme activity, protein-protein interactions, subcelluar localization and protein turnover. The catalytic domains of most eukaryotic protein kinases are conserved in their primary seque ...
Identification of the Factors Responsible for the Interaction of
... Hsp90 and co chaperones like p23, Aha1, Cdc37, Transcription factor like p53 and kinases like Akt, Ask1, Cdk2, Cdk4, Cdk6, Cdk11, DAPK2, ErbB2, Raf-1, B-Raf was investigated. We showed that client proteins and cochaperones contain hydrophobic patches and hence, they are prone to bind with Hsp90 th ...
... Hsp90 and co chaperones like p23, Aha1, Cdc37, Transcription factor like p53 and kinases like Akt, Ask1, Cdk2, Cdk4, Cdk6, Cdk11, DAPK2, ErbB2, Raf-1, B-Raf was investigated. We showed that client proteins and cochaperones contain hydrophobic patches and hence, they are prone to bind with Hsp90 th ...
Characterization of a 30S Ribsomal Subunit Intermediate Found in
... was the next compound of choice. Not surprisingly, within a year of FDA approval daptomycin resistant strains emerged (Mangili and others 2005). A resolution to this problem now focuses on identifying new targets that inhibit essential bacterial functions (Chu and others 1996). However, this has no ...
... was the next compound of choice. Not surprisingly, within a year of FDA approval daptomycin resistant strains emerged (Mangili and others 2005). A resolution to this problem now focuses on identifying new targets that inhibit essential bacterial functions (Chu and others 1996). However, this has no ...
Accumulation of xylem transported protein at pit membranes and
... pathway of least resistance during sap transport from one vessel to another. Nevertheless, they may account for around 50% of overall xylem resistance to flow (see review by Choat et al., 2008). Proteomic investigations using gel separations and, more recently, tandem mass spectrometry have identifi ...
... pathway of least resistance during sap transport from one vessel to another. Nevertheless, they may account for around 50% of overall xylem resistance to flow (see review by Choat et al., 2008). Proteomic investigations using gel separations and, more recently, tandem mass spectrometry have identifi ...
FtsZ - Cytoskeleton, Inc.
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
... cell division. FtsZ inactivation inhibits cell division, making them attractive targets for novel anti-microbial drugs. Although FtsZ proteins exhibit a degree of homology, inhibitors of the proteins show differential affinities and efficacies. Thus, improved targeting can be achieved by screening s ...
THE DYNAMIN SUPERFAMILY: UNIVERSAL MEMBRANE
... as: PLECKSTRIN-HOMOLOGY (PH) DOMAINS; proline-rich domains (PRDs) that bind to SRC-HOMOLOGY-3 (SH3) DOMAINS; or by sequences that target dynamins to specific organelles, such as mitochondria and chloroplasts. It should also be noted that alternative targeting can arise as a result of alternative spl ...
... as: PLECKSTRIN-HOMOLOGY (PH) DOMAINS; proline-rich domains (PRDs) that bind to SRC-HOMOLOGY-3 (SH3) DOMAINS; or by sequences that target dynamins to specific organelles, such as mitochondria and chloroplasts. It should also be noted that alternative targeting can arise as a result of alternative spl ...
Reprint
... All eRF3 proteins described so far contain a variable N terminal domain [37]. Its primary function is unlikely to be to catalyze prion formation, since such a property has not been described for eRF3 in species other than S. cerevisiae. However, this domain is not essential for cell viability in yea ...
... All eRF3 proteins described so far contain a variable N terminal domain [37]. Its primary function is unlikely to be to catalyze prion formation, since such a property has not been described for eRF3 in species other than S. cerevisiae. However, this domain is not essential for cell viability in yea ...
Isolation and Characterization of a Novel Ribosome
... second GSP of 29 bp was designed, corresponding to the DNA sequence located approximately 200 bp downstream of the 3⬘-RACE GSPs. 5⬘-RACE was performed and amplification products were cloned and sequenced as described for the 3⬘-RACE procedures. After DNA sequencing of the multiple clones for each am ...
... second GSP of 29 bp was designed, corresponding to the DNA sequence located approximately 200 bp downstream of the 3⬘-RACE GSPs. 5⬘-RACE was performed and amplification products were cloned and sequenced as described for the 3⬘-RACE procedures. After DNA sequencing of the multiple clones for each am ...
108. Regulation of the L-arabinose Operon in Escherichia coli, in
... Schleif, 1998). This indicated that the AraC protein itself is rigid in the absence of arabinose and cannot adapt to the reoriented O2 half-site. In vivo, AraC was seen to shift from forming a DNA loop to binding to adjacent halfsites when arabinose was added (Martin et al., 1986). In vitro, the con ...
... Schleif, 1998). This indicated that the AraC protein itself is rigid in the absence of arabinose and cannot adapt to the reoriented O2 half-site. In vivo, AraC was seen to shift from forming a DNA loop to binding to adjacent halfsites when arabinose was added (Martin et al., 1986). In vitro, the con ...
The 2009 Nobel Prize in Chemistry and KEK`s Photon Factory
... Yonath, who was simply incapable of sitting around doing nothing with ribosomal crystal in her hands, “those were very long ‘short breaks’.” ...
... Yonath, who was simply incapable of sitting around doing nothing with ribosomal crystal in her hands, “those were very long ‘short breaks’.” ...
12 Insights into the mechanisms underlying CFTR channel activity
... protein kinase) [37–39]. As a result of elegant biochemical, structural and computational studies, we now understand that the R domain is a flexible disordered region of the protein which undergoes dynamic interactions with other CFTR domains [40,41]. Although this domain is disordered, it possesses ...
... protein kinase) [37–39]. As a result of elegant biochemical, structural and computational studies, we now understand that the R domain is a flexible disordered region of the protein which undergoes dynamic interactions with other CFTR domains [40,41]. Although this domain is disordered, it possesses ...
Ciliary Microtubule Capping Structures Contain A
... central pair cap is separated from the stained caps at the ends of the A-microtubules (Fig. 5 B). The majority of the axoneme (small arrow) is stained at a background level as are the cap-free axonemes (open arrows), and cilia stained only with the secondary antibody (Fig. 5 I). To insure that the l ...
... central pair cap is separated from the stained caps at the ends of the A-microtubules (Fig. 5 B). The majority of the axoneme (small arrow) is stained at a background level as are the cap-free axonemes (open arrows), and cilia stained only with the secondary antibody (Fig. 5 I). To insure that the l ...
Functional and structural studies of a C
... synthesize proteins on a continuous basis at an incredible speed of > 15 peptide bonds formed per second. Despite their size difference (prokaryotic: 70S, ~ 2.5 MDa; eukaryotic: 80S, ~ 4 MDa), ribosomes in all kingdoms of life are functionally conserved, with the highest level of sequence conservati ...
... synthesize proteins on a continuous basis at an incredible speed of > 15 peptide bonds formed per second. Despite their size difference (prokaryotic: 70S, ~ 2.5 MDa; eukaryotic: 80S, ~ 4 MDa), ribosomes in all kingdoms of life are functionally conserved, with the highest level of sequence conservati ...
Protein targeting, translocation and Escherichia coli Proteomic analysis of substrate-pathway relationships
... Single spanning α-helical membrane proteins have of one TM domain, while multispanning α-helical membrane proteins have of two or more TM domains connected together by hydrophilic loop regions. The loop regions can be short linkers consisting of just a few amino acids or they can consist of a long ...
... Single spanning α-helical membrane proteins have of one TM domain, while multispanning α-helical membrane proteins have of two or more TM domains connected together by hydrophilic loop regions. The loop regions can be short linkers consisting of just a few amino acids or they can consist of a long ...
Intraflagellar transport molecules in ciliary and nonciliary cells of the
... raised to murine IFT proteins (Pazour et al., 2002; Baker et al., 2003; Follit et al., 2006). These experiments verified the expression of all five IFT proteins in the murine retina (Fig. S1, A and B). The antibodies to four IFT proteins each recognized a single band of expected size in retinal prot ...
... raised to murine IFT proteins (Pazour et al., 2002; Baker et al., 2003; Follit et al., 2006). These experiments verified the expression of all five IFT proteins in the murine retina (Fig. S1, A and B). The antibodies to four IFT proteins each recognized a single band of expected size in retinal prot ...
assembly of integral membrane proteins from the periplasm into the
... Schulz, 2002). Some examples for OMPs are given in Table 1, which also lists the molecular weight, the pI, the number of "-strands, the number of amino acid residues, the oligomeric state, and the function of the OMP. Structures of several OMPs are shown in Color Plate 2. "-Barrel membrane proteins ...
... Schulz, 2002). Some examples for OMPs are given in Table 1, which also lists the molecular weight, the pI, the number of "-strands, the number of amino acid residues, the oligomeric state, and the function of the OMP. Structures of several OMPs are shown in Color Plate 2. "-Barrel membrane proteins ...
Allosteric Interactions after 50Years
... Buc’s illuminating account, including Monod’s resistance to equivalent monomers, however hypothetical they might be, because they did not respect the principle of quaternary constraint. 30 Conceptual aspects of these distinctions are further detailed elsewhere in this issue. 20 Numerous pertinent eq ...
... Buc’s illuminating account, including Monod’s resistance to equivalent monomers, however hypothetical they might be, because they did not respect the principle of quaternary constraint. 30 Conceptual aspects of these distinctions are further detailed elsewhere in this issue. 20 Numerous pertinent eq ...
Chapter 5 Photosynthesis
... a reaction cycle performed by cytochrome b6 f that couples electron transfer to proton transfer. Several models for the reaction sequences exist (Cramer & Knaff, 1991). A similar Qcycle exists in the mitochondrial electron-transfer chain (Brandt & Trumpower, 1994; Brandt, 1996). The function of cyto ...
... a reaction cycle performed by cytochrome b6 f that couples electron transfer to proton transfer. Several models for the reaction sequences exist (Cramer & Knaff, 1991). A similar Qcycle exists in the mitochondrial electron-transfer chain (Brandt & Trumpower, 1994; Brandt, 1996). The function of cyto ...
NF-kappaB : mini revisão The Rel/NF
... As described above, the structures of several Rel/NF-kB dimers on DNA or bound to IkB are known. In all cases, these structures have been derived from molecules that contain almost exclusively residues from the RH domain. As such, these studies provide rather static glimpses of these factors at work ...
... As described above, the structures of several Rel/NF-kB dimers on DNA or bound to IkB are known. In all cases, these structures have been derived from molecules that contain almost exclusively residues from the RH domain. As such, these studies provide rather static glimpses of these factors at work ...
Differential actin binding along the PEVK domain of skeletal muscle
... Titin has been shown previously to bind actin (Kellermayer and Granzier, 1996; Maruyama et al., 1987; Soteriou et al., 1993). Several different segments or elements of the molecule were demonstrated to possess independent actin-binding properties. Near the Z-line, titin is tightly associated with a ...
... Titin has been shown previously to bind actin (Kellermayer and Granzier, 1996; Maruyama et al., 1987; Soteriou et al., 1993). Several different segments or elements of the molecule were demonstrated to possess independent actin-binding properties. Near the Z-line, titin is tightly associated with a ...
enterocolitica Yersinia Type III Secretion System of Properties of the
... enzymes, but also forms a barrier to the secretion of proteins and uptake of nutrients. The passage of small hydrophilic molecules across the outer membrane is facilitated by the presence of a large number of proteinaceous channels, formed by general and substrate-specific porins and TonB-dependent ...
... enzymes, but also forms a barrier to the secretion of proteins and uptake of nutrients. The passage of small hydrophilic molecules across the outer membrane is facilitated by the presence of a large number of proteinaceous channels, formed by general and substrate-specific porins and TonB-dependent ...
LS1a Fall 2014 Lab 6: Ribosomal Protein Translation (PyMOL lab #3)
... Close your PyMOL window. Open the file “2-ElongationFactors.pse” We have just examined a structure of the ribosome in the process of elongating a polypeptide strand, but we left out two of the most important players: EF-Tu and EF-G. Section 2a: EF-Tu EF-Tu and EF-G are both GTPases that intrinsi ...
... Close your PyMOL window. Open the file “2-ElongationFactors.pse” We have just examined a structure of the ribosome in the process of elongating a polypeptide strand, but we left out two of the most important players: EF-Tu and EF-G. Section 2a: EF-Tu EF-Tu and EF-G are both GTPases that intrinsi ...
pdf file - John Innes Centre
... formation between GlnK and AmtB was obtained by purification of the intact complex from the membrane fraction of E. coli cells that had been subjected to a prior ammonium shock (15). A series of subsequent studies in other bacteria supported the proposition that this interaction is conserved among p ...
... formation between GlnK and AmtB was obtained by purification of the intact complex from the membrane fraction of E. coli cells that had been subjected to a prior ammonium shock (15). A series of subsequent studies in other bacteria supported the proposition that this interaction is conserved among p ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.