Solubility of recombinant Src homology 2 domains expressed in E
... of beta-aggregation stretches in proteins [13], TANGO (http://tango.crg.es). TANGO analysis of the human TSAd SH2 domain sequence (S90-R188) revealed that the βC strand of the human TSAd SH2 domain (Figure 1) harbours a nine aa sequence (SAVTFVLTY) with near 100% propensity for intermolecular beta-s ...
... of beta-aggregation stretches in proteins [13], TANGO (http://tango.crg.es). TANGO analysis of the human TSAd SH2 domain sequence (S90-R188) revealed that the βC strand of the human TSAd SH2 domain (Figure 1) harbours a nine aa sequence (SAVTFVLTY) with near 100% propensity for intermolecular beta-s ...
A Personal Account of Chaperonin History
... these observations were made immediately after Anfinsen’s seminal work (1) that provided the dominating intellectual framework in the 1970s and 1980s in all matters related to protein folding. By then the prevailing dogma was that protein folding was a spontaneous event and suggesting that the foldi ...
... these observations were made immediately after Anfinsen’s seminal work (1) that provided the dominating intellectual framework in the 1970s and 1980s in all matters related to protein folding. By then the prevailing dogma was that protein folding was a spontaneous event and suggesting that the foldi ...
Calcium binding chaperones of the endoplasmic reticulum
... Grp94 is highly expressed in the early stages of embryonic hearts and suggests that it may have a role in the process of myocardial cell differentiation and heart development (Barnes and Smoak 1997). It has been found that the selective increase in Grp94 in response to Ca2+ levels protects cardiomyo ...
... Grp94 is highly expressed in the early stages of embryonic hearts and suggests that it may have a role in the process of myocardial cell differentiation and heart development (Barnes and Smoak 1997). It has been found that the selective increase in Grp94 in response to Ca2+ levels protects cardiomyo ...
The Sad1-UNC-84 homology domain in Mps3 interacts with Mps2 to
... Cdc31, Kar1, Mps3, and Sfi1. Kar1 and Mps3 are integral membrane proteins that localize to the cytoplasmic and nuclear sides of the half-bridge, respectively (Spang et al., 1995; Jaspersen et al., 2002; Nishikawa et al., 2003), whereas Cdc31 and Sfi1 are soluble half-bridge components (Spang et al., ...
... Cdc31, Kar1, Mps3, and Sfi1. Kar1 and Mps3 are integral membrane proteins that localize to the cytoplasmic and nuclear sides of the half-bridge, respectively (Spang et al., 1995; Jaspersen et al., 2002; Nishikawa et al., 2003), whereas Cdc31 and Sfi1 are soluble half-bridge components (Spang et al., ...
The Structure of Human Prions: From Biology to Structural Models
... Low-resolution biophysical and biochemical techniques have been used by many research groups to gain insights into the conformations of both PrPC and PrPSc, however these approaches have limitations as will be discussed below. PrPC and PrPSc differ regarding their solubility, fibril formation tenden ...
... Low-resolution biophysical and biochemical techniques have been used by many research groups to gain insights into the conformations of both PrPC and PrPSc, however these approaches have limitations as will be discussed below. PrPC and PrPSc differ regarding their solubility, fibril formation tenden ...
The Pseudomonas syringae HrpJ protein controls the secretion of
... translocation of effectors (Iriarte et al., 1998; Day et al., 2003). Salmonella enterica mutants lacking InvE or SsaL, YopN-TyeA/InvE/SepL family members of the two T3SSs of S. enterica, do not secrete type III translocator proteins (Kubori and Galán, 2002; Coombes et al., 2004). SepL from enteropat ...
... translocation of effectors (Iriarte et al., 1998; Day et al., 2003). Salmonella enterica mutants lacking InvE or SsaL, YopN-TyeA/InvE/SepL family members of the two T3SSs of S. enterica, do not secrete type III translocator proteins (Kubori and Galán, 2002; Coombes et al., 2004). SepL from enteropat ...
A pollen-specific calmodulin-binding protein, NPG1, interacts with
... gradient at the tube tip is observed in growing pollen tubes by ratiometric Ca21 imaging and pollen tube growth can be inhibited when the apical Ca21 gradient is disturbed by blocking calcium uptake13,16–19. In plant species where pollen tube growth displays clear oscillations, it has been shown tha ...
... gradient at the tube tip is observed in growing pollen tubes by ratiometric Ca21 imaging and pollen tube growth can be inhibited when the apical Ca21 gradient is disturbed by blocking calcium uptake13,16–19. In plant species where pollen tube growth displays clear oscillations, it has been shown tha ...
Anal. Chem. (2011) - American Chemical Society
... approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative cleavage of peptides at the C-terminus of tyrosine residues was performed at potentials between 0.5 and 1.5 V (vs a quasi palladium (Pd) reference electrode).27 A recent detailed stu ...
... approach was performed on peptides27 and proteins28 online with ESI-MS detection. Efficient electrochemical oxidative cleavage of peptides at the C-terminus of tyrosine residues was performed at potentials between 0.5 and 1.5 V (vs a quasi palladium (Pd) reference electrode).27 A recent detailed stu ...
Removal of SDS from biological protein digests for proteomic
... studies. SDS is widely used and considered to be very beneficial due to complete cell lysis, disaggregation and efficient solubilization of the global proteome primarily hydrophobic membrane-bound components. SDS interacted with proteins by ionic and hydrophobic bonds and dissolves proteins by chang ...
... studies. SDS is widely used and considered to be very beneficial due to complete cell lysis, disaggregation and efficient solubilization of the global proteome primarily hydrophobic membrane-bound components. SDS interacted with proteins by ionic and hydrophobic bonds and dissolves proteins by chang ...
Structural studies on lysosomal proteins
... and when the hydrolysis takes place. Thus, degradation can be used both for recycling and regulation purposes. The lysosomal lumen is now known to contain more than 60 soluble lysosomal hydrolases. They are a versatile group of mostly unrelated proteins, of which some are quite well characterised, s ...
... and when the hydrolysis takes place. Thus, degradation can be used both for recycling and regulation purposes. The lysosomal lumen is now known to contain more than 60 soluble lysosomal hydrolases. They are a versatile group of mostly unrelated proteins, of which some are quite well characterised, s ...
Intestinal absorption of amino acids and peptides
... Competition for intestinal transport and transport groups T h e results of animal experiments, in particular experiments on competition for intestinal transport (usually in rat or hamster), supported by observations on intestinal transport defects in man, have suggested the existence of several ‘tra ...
... Competition for intestinal transport and transport groups T h e results of animal experiments, in particular experiments on competition for intestinal transport (usually in rat or hamster), supported by observations on intestinal transport defects in man, have suggested the existence of several ‘tra ...
ENS’06 FUSION PHAGE AS A BIOSELECTIVE NANOMATERIAL: EVOLUTION OF THE CONCEPT
... enters the cytoplasm [12]. The protein is synthesized in infected cell as a water-soluble precursor, which contains a leader sequence of 23 residues at its N-terminus. When this protein is inserted into the membrane, the leader sequence is cleaved off by a leader peptidase. Later, during the phage a ...
... enters the cytoplasm [12]. The protein is synthesized in infected cell as a water-soluble precursor, which contains a leader sequence of 23 residues at its N-terminus. When this protein is inserted into the membrane, the leader sequence is cleaved off by a leader peptidase. Later, during the phage a ...
Structure and mechanism of ATP-dependent phospholipid transporters
... Major conclusions: Both protein families hydrolyze ATP, although they employ different mechanisms to use it, and have a comparable size with twelve transmembrane segments in the functional unit. Further, despite differences in overall architecture, both appear to operate by an alternating access mec ...
... Major conclusions: Both protein families hydrolyze ATP, although they employ different mechanisms to use it, and have a comparable size with twelve transmembrane segments in the functional unit. Further, despite differences in overall architecture, both appear to operate by an alternating access mec ...
Merlin and Expanded interact - Development
... Although transmembrane partners for Merlin have not been identified yet, the hyaluronin receptor CD44, the adhesion molecule ICAM-2, the cystic fibrosis transmembrane conductance regulator and the B-adrenergic receptor have been shown to bind ERM proteins directly or indirectly (Tsukita et al., 1994 ...
... Although transmembrane partners for Merlin have not been identified yet, the hyaluronin receptor CD44, the adhesion molecule ICAM-2, the cystic fibrosis transmembrane conductance regulator and the B-adrenergic receptor have been shown to bind ERM proteins directly or indirectly (Tsukita et al., 1994 ...
PDF
... Transplantation of single CaPs and MiPs revealed that their subtypes are initially labile and responsive to environmental signals, but become committed shortly before axogenesis (Eisen, 1991), around the time that the alternating pattern of islet2 and islet1 expression is established (Appel et al., ...
... Transplantation of single CaPs and MiPs revealed that their subtypes are initially labile and responsive to environmental signals, but become committed shortly before axogenesis (Eisen, 1991), around the time that the alternating pattern of islet2 and islet1 expression is established (Appel et al., ...
Molecular architecture of the glomerular slit
... provided support for the hypothesis that the outermost N-terminal Ig-like domains of nephrin from aadjacent podocytes interact homotypically [11,12], linking the foot processes dynamically together. This interaction most likely provides the structural but also functional scaffold for the slit diaphr ...
... provided support for the hypothesis that the outermost N-terminal Ig-like domains of nephrin from aadjacent podocytes interact homotypically [11,12], linking the foot processes dynamically together. This interaction most likely provides the structural but also functional scaffold for the slit diaphr ...
Escherichia coli Karl Skoog
... cytoplasm, whereas the second step, polymerization of peptidoglycan precursors to form the peptidoglycan layer, occurs in the periplasm 35. The peptidoglycan precursor is called lipid II. It is a disaccharide pentapeptide linked to an undecaprenylphosphate lipid and it is formed by a number of event ...
... cytoplasm, whereas the second step, polymerization of peptidoglycan precursors to form the peptidoglycan layer, occurs in the periplasm 35. The peptidoglycan precursor is called lipid II. It is a disaccharide pentapeptide linked to an undecaprenylphosphate lipid and it is formed by a number of event ...
Copyright Information of the Article Published Online
... The origin of mitochondria: The origin of mitochondria is more likely to be endosymbiotic. The endosymbiotic theory indicates that mitochondria were initially free-living prokaryotes that entered eukaryotic cells and became organelles. However, this theory has not always been accepted by the scienti ...
... The origin of mitochondria: The origin of mitochondria is more likely to be endosymbiotic. The endosymbiotic theory indicates that mitochondria were initially free-living prokaryotes that entered eukaryotic cells and became organelles. However, this theory has not always been accepted by the scienti ...
Specific Interaction of the PDZ Domain Protein PICK1 with the
... following the glutamine at position 669 in PKCa. This mutant, GAL4(DB)-PKC7(D670 – 672), failed to interact with PICK1 in the yeast two-hybrid assay (Fig. 2B). We then mutated the four COOH-terminal amino acids in PKCa to alanines. This mutant, GAL4(DB)-PKC7(QSAV669 – 672AAAA), also failed to intera ...
... following the glutamine at position 669 in PKCa. This mutant, GAL4(DB)-PKC7(D670 – 672), failed to interact with PICK1 in the yeast two-hybrid assay (Fig. 2B). We then mutated the four COOH-terminal amino acids in PKCa to alanines. This mutant, GAL4(DB)-PKC7(QSAV669 – 672AAAA), also failed to intera ...
Calretinin
... connecting the two helices. • In this loop, there are amino acid residues with negatively charged oxygen atoms, which attract the positively charged calcium ion • When Ca2+ enters the cell Calretinin binds about 99% of it. ...
... connecting the two helices. • In this loop, there are amino acid residues with negatively charged oxygen atoms, which attract the positively charged calcium ion • When Ca2+ enters the cell Calretinin binds about 99% of it. ...
Membrane Proteins
... Models of Membrane Structure: An Experimental Approach • The development of electron microscopy in the 1950s was important for understanding membrane structure ...
... Models of Membrane Structure: An Experimental Approach • The development of electron microscopy in the 1950s was important for understanding membrane structure ...
Post-Mortem Chemical Changes in Muscle
... rigor also occurs in muscles from stressed animals - muscles in which there is little or no accumulation of lactic acid. This observation led to a thorough reappraisal of the phenomenon of rigor mortis, and, arising from the developing knowledge of the contractile process, it was soon evident that t ...
... rigor also occurs in muscles from stressed animals - muscles in which there is little or no accumulation of lactic acid. This observation led to a thorough reappraisal of the phenomenon of rigor mortis, and, arising from the developing knowledge of the contractile process, it was soon evident that t ...
Selected Reaction Monitoring (SRM) to determine protein
... no transcript, while 42% resulted in reduced transcript abundance and 14% showed no change or an increase in transcript abundance (Wang, 2008). Of these studies, only 136 reported information on abundance of the protein encoded by the gene of interest and of these 80% showed no protein expression wh ...
... no transcript, while 42% resulted in reduced transcript abundance and 14% showed no change or an increase in transcript abundance (Wang, 2008). Of these studies, only 136 reported information on abundance of the protein encoded by the gene of interest and of these 80% showed no protein expression wh ...
WORD
... A lot of studies have been done on one specific BCAA, Leucine. Leucine is one of the amino acids that’s predominantly responsible for muscle protein synthesis. In most BCAA supplements, Leucine will be the heaviest dose, perhaps in a typical ratio of 2:1:1. In a doubleblind, randomized trial study, ...
... A lot of studies have been done on one specific BCAA, Leucine. Leucine is one of the amino acids that’s predominantly responsible for muscle protein synthesis. In most BCAA supplements, Leucine will be the heaviest dose, perhaps in a typical ratio of 2:1:1. In a doubleblind, randomized trial study, ...
Introduction - ART
... Ubiquitin ligases that carry a RING finger domain exist as multisubunit complexes, or as monomers with substrate binding information and E3 activity built into the same molecule (Joazeiro et al., 2000). Genome sequencing suggests that RING finger E3s may far out number their HECT domain counterparts ...
... Ubiquitin ligases that carry a RING finger domain exist as multisubunit complexes, or as monomers with substrate binding information and E3 activity built into the same molecule (Joazeiro et al., 2000). Genome sequencing suggests that RING finger E3s may far out number their HECT domain counterparts ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.