Modular evolution of phosphorylation
... ubiquitylation and transcription [27]. SH2 domains therefore provide a common mechanism for proteins with entirely different biochemical properties to couple to upstream pTyr signals. The transmission of information in signalling pathways is further enhanced by many different classes of interaction ...
... ubiquitylation and transcription [27]. SH2 domains therefore provide a common mechanism for proteins with entirely different biochemical properties to couple to upstream pTyr signals. The transmission of information in signalling pathways is further enhanced by many different classes of interaction ...
fulltekst
... From gene to protein All complex organisms, like an animal or a plant, are built up of small specified cells forming different tissues that together create the organism. Within its cell nucleus, each cell contains identical genomic material that describes the construction of different proteins perfo ...
... From gene to protein All complex organisms, like an animal or a plant, are built up of small specified cells forming different tissues that together create the organism. Within its cell nucleus, each cell contains identical genomic material that describes the construction of different proteins perfo ...
Conserved mechanisms of Ras regulation of evolutionary
... Figure 3), whereas the individual activation domains RI and RIII alone had very low activity [less than twothreefold, compare G-RI and G-RIII with GaI4 (1 ± 147) and see (Schneikert et al., 1992)]. The regulatory domain RII was inactive on its own (G-RII), whereas combining it with RI or RIII led to ...
... Figure 3), whereas the individual activation domains RI and RIII alone had very low activity [less than twothreefold, compare G-RI and G-RIII with GaI4 (1 ± 147) and see (Schneikert et al., 1992)]. The regulatory domain RII was inactive on its own (G-RII), whereas combining it with RI or RIII led to ...
Team Publications
... and precedes ADP release. Increasing magnesium concentration to within the physiological range would thus slow both the ATPase activity and the velocity of movement of this motor. ...
... and precedes ADP release. Increasing magnesium concentration to within the physiological range would thus slow both the ATPase activity and the velocity of movement of this motor. ...
Protein degradation in mitochondria
... mitochondrial proteins have been observed in yeast mitochondria lacking PIM1 protease.22 It is conceivable that the ATPase domain of Lon proteases exerts chaperone-like activity, promotes substrate unfolding and ensures the specificity of proteolysis, as such a role has been demonstrated for structu ...
... mitochondrial proteins have been observed in yeast mitochondria lacking PIM1 protease.22 It is conceivable that the ATPase domain of Lon proteases exerts chaperone-like activity, promotes substrate unfolding and ensures the specificity of proteolysis, as such a role has been demonstrated for structu ...
Biomolecules Review
... • Enzymes speed up reactions by lowering the activation energy. • Proteins ...
... • Enzymes speed up reactions by lowering the activation energy. • Proteins ...
Definition of Protein Superfamily
... Margaret O. Dayhoff introduced the term protein superfamily in 1974 [1,2,3]. Since that time, the sequences in the PIR-International Protein Sequence Database have been classified into protein superfamilies. Prior to about 1990, the superfamily classification permitted a sequence to be assigned to a ...
... Margaret O. Dayhoff introduced the term protein superfamily in 1974 [1,2,3]. Since that time, the sequences in the PIR-International Protein Sequence Database have been classified into protein superfamilies. Prior to about 1990, the superfamily classification permitted a sequence to be assigned to a ...
SILK-BASED DELIVERY OF BIOACTIVE MOLECULES
... polyalanine sequence, controlled self assembly of beta-sheet structures in silk. Modified spider silk, which was 15mer of [SGRGGLGGQGAGAAAAAGGAGQGGYGGLGSQGT] derived from the spidroin was bioengineered to include arginyl-glycyl-aspartic acid(RGD) cell-binding domains to enhance cell adhesion. Al ...
... polyalanine sequence, controlled self assembly of beta-sheet structures in silk. Modified spider silk, which was 15mer of [SGRGGLGGQGAGAAAAAGGAGQGGYGGLGSQGT] derived from the spidroin was bioengineered to include arginyl-glycyl-aspartic acid(RGD) cell-binding domains to enhance cell adhesion. Al ...
Lecture Notes for Methods in Cell Biology
... conclusions of scientific papers. Students anticipating careers involving biological or medical research at any level will benefit from this course. The course consists of three sections: 1) general biochemical and biophysical methods, 2) analysis and isolation of proteins and immunological procedur ...
... conclusions of scientific papers. Students anticipating careers involving biological or medical research at any level will benefit from this course. The course consists of three sections: 1) general biochemical and biophysical methods, 2) analysis and isolation of proteins and immunological procedur ...
Subcellular Trafficking of Mammalian Lysosomal Proteins: An
... transport machineries that transfer cargoes between cellular compartments (reviewed in [10–15]). However, an increasing number of observations indicate that some lysosomal proteins can reach lysosomes by “non-conventional” transport mechanisms. While some of them have been known from quite some time ...
... transport machineries that transfer cargoes between cellular compartments (reviewed in [10–15]). However, an increasing number of observations indicate that some lysosomal proteins can reach lysosomes by “non-conventional” transport mechanisms. While some of them have been known from quite some time ...
Studies on legume receptors for Nod and Myc symbiotic signals
... composition of chimeric receptors, was investigated by combining together the extracellular domain of LYR3 protein with the kinases of Arabidopsis thaliana immune receptors, AtCERK1 and EFR. The results suggest that LYR3 could be used for constructing biologically active chimeric proteins whose mode ...
... composition of chimeric receptors, was investigated by combining together the extracellular domain of LYR3 protein with the kinases of Arabidopsis thaliana immune receptors, AtCERK1 and EFR. The results suggest that LYR3 could be used for constructing biologically active chimeric proteins whose mode ...
Similarities and Differences in the Glycosylation Mechanisms in
... [14]. Interestingly, these workers found that PglK has a relaxed substrate specificity exemplified by its ability to complement a Wzx deficiency in O-antigen biosynthesis in E. coli. Notably, all bacterial N-glycans identified to date have seven or fewer sugar residues, with many archaeal structures ...
... [14]. Interestingly, these workers found that PglK has a relaxed substrate specificity exemplified by its ability to complement a Wzx deficiency in O-antigen biosynthesis in E. coli. Notably, all bacterial N-glycans identified to date have seven or fewer sugar residues, with many archaeal structures ...
Troubleshooting SDS-PAGE-0410
... Glass plates might be chipped. The glass plate might not be aligned well. Use Vaseline at the spacers. There is an excess of heat generation, use cooled reagents. There is insufficient glycerol in the sample buffer. Comb is removed before the stacking gel has properly been polymerized. Let the gel p ...
... Glass plates might be chipped. The glass plate might not be aligned well. Use Vaseline at the spacers. There is an excess of heat generation, use cooled reagents. There is insufficient glycerol in the sample buffer. Comb is removed before the stacking gel has properly been polymerized. Let the gel p ...
Weak acids and bases an isoelectric points of proteins
... scale is a log scale. To fit all of the data of columns A and B of Figure 1 onto the graph shown in Figure 2A, the value of the y-axis had to increase in increments of 2 108. Thus all of the increases in the above ratio (up to pH = 12) appear flat on the graph shown in Figure 2A. Consequently, ou ...
... scale is a log scale. To fit all of the data of columns A and B of Figure 1 onto the graph shown in Figure 2A, the value of the y-axis had to increase in increments of 2 108. Thus all of the increases in the above ratio (up to pH = 12) appear flat on the graph shown in Figure 2A. Consequently, ou ...
Dual-topology membrane proteins Escherichia coli Susanna Seppälä
... Cellular identity relies on the existence of the cellular membrane, a semipermeable barrier that encloses any cell and defines its boundary. In many cells, the interior is further divided into membrane enclosed compartments with specialized functions (organelles), and multicellular organisms are, si ...
... Cellular identity relies on the existence of the cellular membrane, a semipermeable barrier that encloses any cell and defines its boundary. In many cells, the interior is further divided into membrane enclosed compartments with specialized functions (organelles), and multicellular organisms are, si ...
scf and cullin/ring h2-based
... Skowyra et al 1997). Over the past few years, SCF pathway components have been identified and linked to diverse cellular processes in many eukaryotes. In this review, I describe the multiple lines of research that led to the discovery of SCF ubiquitin ligases and review what is known about the subun ...
... Skowyra et al 1997). Over the past few years, SCF pathway components have been identified and linked to diverse cellular processes in many eukaryotes. In this review, I describe the multiple lines of research that led to the discovery of SCF ubiquitin ligases and review what is known about the subun ...
Early and late endosomal compartments of Entamoeba histolytica
... importance of the E. histolytica endo-lysosomal and secretory systems in nutrition and invasion of the organism, relatively little is known about the molecular factors governing these systems including the associated proteins which may regulate endocytic or secretory function. In other eukaryotes, t ...
... importance of the E. histolytica endo-lysosomal and secretory systems in nutrition and invasion of the organism, relatively little is known about the molecular factors governing these systems including the associated proteins which may regulate endocytic or secretory function. In other eukaryotes, t ...
Biogenesis of proteins of the mitochondrial intermembrane space
... classical presequences that are followed by a hydrophobic stretch which eventually arrest the translocation of these proteins at the level of the TIM23 complex. This results in lateral insertion into the inner membrane (Gärtner, Bomer et al. 1995; Rojo, Guiard et al. 1998). Interestingly, in some in ...
... classical presequences that are followed by a hydrophobic stretch which eventually arrest the translocation of these proteins at the level of the TIM23 complex. This results in lateral insertion into the inner membrane (Gärtner, Bomer et al. 1995; Rojo, Guiard et al. 1998). Interestingly, in some in ...
Williams, Brandon: A Review on Common Applications Designed to Optimize the SEQUEST Search Algorithm With Respect to Limiting False Positive Identification
... New proteomic technologies have emerged that deal with understanding complex systems and diseases at a global level, thus allowing an expanded experimental view. Before the advent of mass spectrometry, proteomic approaches were initially explored via the use of two-dimensional gel electrophoresis (1 ...
... New proteomic technologies have emerged that deal with understanding complex systems and diseases at a global level, thus allowing an expanded experimental view. Before the advent of mass spectrometry, proteomic approaches were initially explored via the use of two-dimensional gel electrophoresis (1 ...
prions lecture notes
... induction of these prion phenotypes was directly shown to be caused by excess of the proteins, not an excess of URE2 and SUP35 DNA or mRNA the induction effect could also be obtained when only a portion of the proteins (prion domains) was overexpressed, these domains were also important for prion pr ...
... induction of these prion phenotypes was directly shown to be caused by excess of the proteins, not an excess of URE2 and SUP35 DNA or mRNA the induction effect could also be obtained when only a portion of the proteins (prion domains) was overexpressed, these domains were also important for prion pr ...
here - Dr. Alan Dombkowski
... Disulfide by Design is an application for the rational design of disulfide bonds in proteins. For a given protein structural model, all residue pairs are rapidly assessed for proximity and geometry consistent with disulfide formation, assuming the residues were mutated to cysteines. The output displ ...
... Disulfide by Design is an application for the rational design of disulfide bonds in proteins. For a given protein structural model, all residue pairs are rapidly assessed for proximity and geometry consistent with disulfide formation, assuming the residues were mutated to cysteines. The output displ ...
Site-selective incorporation and ligation of
... product were obtained when using RS under more basic conditions ( pH 7.5–8.5 as opposed to pH 6.5).32 Genetic incorporation of a formylglycine tag Alongside the chemical methods described earlier for installing aldehydes into proteins, enzymatic methods, such as the formylglycine tag, have also been ...
... product were obtained when using RS under more basic conditions ( pH 7.5–8.5 as opposed to pH 6.5).32 Genetic incorporation of a formylglycine tag Alongside the chemical methods described earlier for installing aldehydes into proteins, enzymatic methods, such as the formylglycine tag, have also been ...
Molecular mechanisms of complement evasion: learning from
... convertase of the alternative pathway. In the scheme, the putative binding of C3 to C3bBb is indicated (here, C3 is in grey, with the C3a domain in red; C3b is shown in light blue); the factor B protease fragment Bb (dark blue and green) is associated with the carboxyl terminus of the C3 substrate. ...
... convertase of the alternative pathway. In the scheme, the putative binding of C3 to C3bBb is indicated (here, C3 is in grey, with the C3a domain in red; C3b is shown in light blue); the factor B protease fragment Bb (dark blue and green) is associated with the carboxyl terminus of the C3 substrate. ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.