Traffic between the plant endoplasmic reticulum and Golgi
... compartments [30], or precursors of the prevacuolar compartment. Microscopy supports the existence of a TGNlike structure at the plant Golgi [31,32]. Earlier studies have also pointed to the existence of subdomains of the TGN [33]. The golgin AtGRIP is thought to localize at the transGolgi, although ...
... compartments [30], or precursors of the prevacuolar compartment. Microscopy supports the existence of a TGNlike structure at the plant Golgi [31,32]. Earlier studies have also pointed to the existence of subdomains of the TGN [33]. The golgin AtGRIP is thought to localize at the transGolgi, although ...
The Cutting Edge of Affinity Electrophoresis Technology
... Figure 3. Saccharide affinity electrophoresis. (A) Reversible bonding between a boron compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cell ...
... Figure 3. Saccharide affinity electrophoresis. (A) Reversible bonding between a boron compound and a polysaccharide; (B) the affinity probe MPBA. 5. Supported Molecular Matrix Electrophoresis and Its Application to Affinity Electrophoresis Mucins are viscous glycoproteins produced by epithelial cell ...
Phosphorylation of apoproteins in VLDL, and LDL by protein
... for apo BlOO phosphorylated by CAMP-PKcompared with AMPPK (Figure lb). This indicates that these two kinases phosphorylate different sites. This has also been suggested by reversed phase HPLC separation of tryptic peptides from apo BlOO phosphorylated by either kinase and will be confmed when these ...
... for apo BlOO phosphorylated by CAMP-PKcompared with AMPPK (Figure lb). This indicates that these two kinases phosphorylate different sites. This has also been suggested by reversed phase HPLC separation of tryptic peptides from apo BlOO phosphorylated by either kinase and will be confmed when these ...
Chapter 4 Calsequestrin - Department of Molecular Physiology and
... able to draw Ca 2+ away from the sarcoplasmic Ca 2+ -binding proteins which have Kd values for Ca2+ binding of about 1-5 µM (Greaser et al., 1972b; Kretsinger. 1976). The contest for Ca2+ is, therefore, unequal and Ca 2+ finds itself in the lumen of the sarcoplasmic reticulum There it encounters ano ...
... able to draw Ca 2+ away from the sarcoplasmic Ca 2+ -binding proteins which have Kd values for Ca2+ binding of about 1-5 µM (Greaser et al., 1972b; Kretsinger. 1976). The contest for Ca2+ is, therefore, unequal and Ca 2+ finds itself in the lumen of the sarcoplasmic reticulum There it encounters ano ...
Write on zinc fingers
... Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not, instead binding other metals such as iron or no metal at all. For example, some family m ...
... Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not, instead binding other metals such as iron or no metal at all. For example, some family m ...
Genetic Dissection of Chloroplast Biogenesis and
... latter it regulates phytochrome-mediated transcription, as it is required for the regulation of PIF1 and PIF3 protein accumulation. Loss of this protein results in an albino phenotype that can only be rescued when both the chloroplast and the nuclear-targeting signals are accessible (Chen et al., 20 ...
... latter it regulates phytochrome-mediated transcription, as it is required for the regulation of PIF1 and PIF3 protein accumulation. Loss of this protein results in an albino phenotype that can only be rescued when both the chloroplast and the nuclear-targeting signals are accessible (Chen et al., 20 ...
Complete atomic model of the bacterial flagellar filament by electron
... The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagell ...
... The bacterial flagellar filament is a helical propeller for bacterial locomotion. It is a helical assembly of a single protein, flagellin, and its tubular structure is formed by 11 protofilaments in two distinct conformations, L- and R-type, for supercoiling. The X-ray crystal structure of a flagell ...
M6PRs are found in a subset of PC12 cell ISGs
... Fig. 1. Subcellular fractionation reveals that both the CI- and the CDM6PR are found in fractions which contain ISGs but not MSGs. (A) Fractions from a velocity sucrose gradient of a post-nuclear supernatant obtained from PC12 cells were immunoblotted with antibodies to the CI- and CD-M6PRs. The loc ...
... Fig. 1. Subcellular fractionation reveals that both the CI- and the CDM6PR are found in fractions which contain ISGs but not MSGs. (A) Fractions from a velocity sucrose gradient of a post-nuclear supernatant obtained from PC12 cells were immunoblotted with antibodies to the CI- and CD-M6PRs. The loc ...
Transcriptional control by the TGF/Smad signaling system
... that activates Smads, and the Smads assemble multisubunit complexes that regulate transcription (Figure 1) (reviewed in MassagueÂ, 1998). Two general steps thus suf®ce to carry the hormonal stimulus to target genes. How can such a simple system mediate a variety of cellspeci®c responses? An incoming ...
... that activates Smads, and the Smads assemble multisubunit complexes that regulate transcription (Figure 1) (reviewed in MassagueÂ, 1998). Two general steps thus suf®ce to carry the hormonal stimulus to target genes. How can such a simple system mediate a variety of cellspeci®c responses? An incoming ...
Diversity of Amyloid Motifs in NLR Signaling in Fungi
... activity in contrast to TIR and death-fold family domains which function as adaptor domains. NB-ARC and NACHT domains can be found in almost all combinations with the various types of N-terminal effector domains and WD, ANK or TPR domains, suggesting that the different domain architectures arise fro ...
... activity in contrast to TIR and death-fold family domains which function as adaptor domains. NB-ARC and NACHT domains can be found in almost all combinations with the various types of N-terminal effector domains and WD, ANK or TPR domains, suggesting that the different domain architectures arise fro ...
Leukaemia Section t(4;17)(q12;q21) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... amino-terminal amino acids of FIP1L, including the FIP homology domain and 403 carboxyl-terminal amino acids of RARA, including the DNA and ligand binding domains, with replacement of FIP1L1 amino acid 429 (Valine) and RARA amino acid 60 (Threonine) into an Alanine. Oncogenesis All known chimeric RA ...
... amino-terminal amino acids of FIP1L, including the FIP homology domain and 403 carboxyl-terminal amino acids of RARA, including the DNA and ligand binding domains, with replacement of FIP1L1 amino acid 429 (Valine) and RARA amino acid 60 (Threonine) into an Alanine. Oncogenesis All known chimeric RA ...
Mechanisms of amino acid sensing in mTOR signaling pathway
... must be recognized at the plasma membrane or within an associated vacuole. Different group, however, reported that only after over 1 hour treatment of the Leu8-MAP could phosphorylate S6 in some degree and moreover, in rat adipocytes, S6K1 and 4EBP1 phosphorylation was unresponsive to Leu8-MAP or IV ...
... must be recognized at the plasma membrane or within an associated vacuole. Different group, however, reported that only after over 1 hour treatment of the Leu8-MAP could phosphorylate S6 in some degree and moreover, in rat adipocytes, S6K1 and 4EBP1 phosphorylation was unresponsive to Leu8-MAP or IV ...
Two Microtubule-Associated Proteins of the
... a unique role of AtMAP65-3/PLEIADE as several mutant alleles of the gene cause failure in cytokinesis by altering microtubule organization in the phragmoplast (Muller et al., 2004). To date, it has been concluded that MAP65 bundles microtubules by forming cross-bridges along neighboring microtubules ...
... a unique role of AtMAP65-3/PLEIADE as several mutant alleles of the gene cause failure in cytokinesis by altering microtubule organization in the phragmoplast (Muller et al., 2004). To date, it has been concluded that MAP65 bundles microtubules by forming cross-bridges along neighboring microtubules ...
as PDF
... methods enable the separation of complex protein mixtures on a single gel. Proteins are separated in two dimensions: in the first dimension, they are fractionated according to their isoelectric point using a pH gradient gel, which is subsequently placed on a polyacrylamide gel slab for further separ ...
... methods enable the separation of complex protein mixtures on a single gel. Proteins are separated in two dimensions: in the first dimension, they are fractionated according to their isoelectric point using a pH gradient gel, which is subsequently placed on a polyacrylamide gel slab for further separ ...
Filip Jagodzinski - WWU Computer Science Faculty Web Pages
... Department Colloquium, Computer Science, UMass Boston, February 2012 Filip Jagodzinski, Grid Rainfall Disaggregation Toward a Patch-Based Ensemble Kalman Filter for Soil Moisture Data Assimilation. White-paper, Goddard Earth Science and Technology Center (GSSP), ...
... Department Colloquium, Computer Science, UMass Boston, February 2012 Filip Jagodzinski, Grid Rainfall Disaggregation Toward a Patch-Based Ensemble Kalman Filter for Soil Moisture Data Assimilation. White-paper, Goddard Earth Science and Technology Center (GSSP), ...
A specific domain in α-catenin mediates binding to β
... with 20 mM Hepes-NaOH, pH 7.4, and ultracentrifuged for 30 minutes at 4°C at 45,000 rpm in an AH650 swing-out rotor. The supernatant was applied to 2 ml amylose resin (New England Biolabs) and incubated for 15 minutes at 4°C on a rotatory shaker. To achieve complete binding the flow-through was reap ...
... with 20 mM Hepes-NaOH, pH 7.4, and ultracentrifuged for 30 minutes at 4°C at 45,000 rpm in an AH650 swing-out rotor. The supernatant was applied to 2 ml amylose resin (New England Biolabs) and incubated for 15 minutes at 4°C on a rotatory shaker. To achieve complete binding the flow-through was reap ...
The SR Protein SRp38 Represses Splicing in M Phase Cells
... of which approximately a dozen have been described in mammalian systems, are modular, containing one or two N-terminal RNP-type RNA binding domains (RBD) and a C-terminal region consisting largely of multiple Arg-Ser dipeptide repeats (RS domain). They play multiple roles in splicing and have the un ...
... of which approximately a dozen have been described in mammalian systems, are modular, containing one or two N-terminal RNP-type RNA binding domains (RBD) and a C-terminal region consisting largely of multiple Arg-Ser dipeptide repeats (RS domain). They play multiple roles in splicing and have the un ...
Hemoglobin Receptor in Leishmania Is a Hexokinase Located in the
... essential for targeting the protein-bound Hb to the late compartment (9), probably to generate intracellular heme. Moreover, a few receptor systems have been identified on trypanosomatid parasites such as Trypanosoma and Leishmania that mediate the uptake of low density lipoprotein and transferrin, ...
... essential for targeting the protein-bound Hb to the late compartment (9), probably to generate intracellular heme. Moreover, a few receptor systems have been identified on trypanosomatid parasites such as Trypanosoma and Leishmania that mediate the uptake of low density lipoprotein and transferrin, ...
Sample pages 1 PDF
... A current list of root-knot and cyst nematode genes with known putative functions in parasitism, mostly based on similarities to characterized proteins in other organisms, is presented in a recent review (8) and shall not be repeated here. In addition to the parasitism proteins with similarity to ch ...
... A current list of root-knot and cyst nematode genes with known putative functions in parasitism, mostly based on similarities to characterized proteins in other organisms, is presented in a recent review (8) and shall not be repeated here. In addition to the parasitism proteins with similarity to ch ...
![Basic region of residues 228-231 of protein kinase CK1[alpha] is](http://s1.studyres.com/store/data/015745345_1-81da768c37e720623b4b1898472e2f2e-300x300.png)
Basic region of residues 228-231 of protein kinase CK1[alpha] is
... Fish et al., 1995]. Some isoforms, such as CK1a, have several splicing variants that have different biochemical and cellular properties [Burzio et al., 2002]. In this article, results are presented which demonstrate that a basic lysine-rich region which is localized in residues 228–231 of CK1a is ne ...
... Fish et al., 1995]. Some isoforms, such as CK1a, have several splicing variants that have different biochemical and cellular properties [Burzio et al., 2002]. In this article, results are presented which demonstrate that a basic lysine-rich region which is localized in residues 228–231 of CK1a is ne ...
Warfarin - Tryptophan.net
... These include blood coagulation factors II (prothrombin), VII, IX, ...
... These include blood coagulation factors II (prothrombin), VII, IX, ...
Field Guide to Protein Folds
... The cyclin-box is an approximately 100-residue domain found in all cyclin and cyclin-like domains that acts as a generalized adaptor motif to recognize diverse proteins and DNAs that are involved in cell cycle and transcriptional regulation. It consists of five helices with a central helix (helix 3) ...
... The cyclin-box is an approximately 100-residue domain found in all cyclin and cyclin-like domains that acts as a generalized adaptor motif to recognize diverse proteins and DNAs that are involved in cell cycle and transcriptional regulation. It consists of five helices with a central helix (helix 3) ...
Combining docking and molecular dynamic simulations in drug design
... docking techniques. While the manifold of conformational structures of small molecules may be relatively easy to predict, the lowest energy conformation obtained may not correspond to that of the bound ligand. The structures of proteins present a bigger challenge. Although experimental techniques in ...
... docking techniques. While the manifold of conformational structures of small molecules may be relatively easy to predict, the lowest energy conformation obtained may not correspond to that of the bound ligand. The structures of proteins present a bigger challenge. Although experimental techniques in ...
Marginally hydrophobic transmembrane helices shaping membrane protein folding
... groups become the amide and keto groups of the peptide backbone, which are hydrophilic but substantially less so than the original charged groups. The aminoacid side chains may be polar, charged or hydrophobic. In addition, the folding and geometry of the protein can make the various groups inaccess ...
... groups become the amide and keto groups of the peptide backbone, which are hydrophilic but substantially less so than the original charged groups. The aminoacid side chains may be polar, charged or hydrophobic. In addition, the folding and geometry of the protein can make the various groups inaccess ...
Immunoblot Detection of Proteins That Contain Cysteine
... pathological conditions such as inflammation and ischemiareperfusion. The generation of ROS also appears to be required for many normal cellular functions, including transduction of cell surface receptor signaling. Thiols of cysteine residues in proteins are the most common targets of ROS in cells. ...
... pathological conditions such as inflammation and ischemiareperfusion. The generation of ROS also appears to be required for many normal cellular functions, including transduction of cell surface receptor signaling. Thiols of cysteine residues in proteins are the most common targets of ROS in cells. ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.