Structural Mechanisms for Regulation of Membrane
... in domain I remains unclear; however, it might in principle serve as an intermediate docking site during prenyl group transfer (6) or perhaps as an alternative pocket for a subset of Rab GTPases. The structural observations and interaction analyses suggest a sequential allosteric model for extractio ...
... in domain I remains unclear; however, it might in principle serve as an intermediate docking site during prenyl group transfer (6) or perhaps as an alternative pocket for a subset of Rab GTPases. The structural observations and interaction analyses suggest a sequential allosteric model for extractio ...
Document
... Tau exhibits disordered features over its entire protein length (441 amino acid residues, 45 kDa) [38] and in contrast to other IDPs, has only weak propensities for transient secondary structures (turn conformations and bstrands) [39,40]. The biological function of Tau is to bind and stabilize micr ...
... Tau exhibits disordered features over its entire protein length (441 amino acid residues, 45 kDa) [38] and in contrast to other IDPs, has only weak propensities for transient secondary structures (turn conformations and bstrands) [39,40]. The biological function of Tau is to bind and stabilize micr ...
Holding it all together? Candidate proteins for the plant Golgi matrix
... that have sequence similarity to a subset of the mammalian or yeast golgins (Table 1). Because the coiled-coil domains show very low levels of sequence conservation in this class of proteins, most of the possible plant homologues were identified on the basis of the presence of other conserved domain ...
... that have sequence similarity to a subset of the mammalian or yeast golgins (Table 1). Because the coiled-coil domains show very low levels of sequence conservation in this class of proteins, most of the possible plant homologues were identified on the basis of the presence of other conserved domain ...
Peptides
... -Peptides are distinguished from proteins on the basis of size, typically containing fewer than 50 monomer (AA) units. -The shortest peptides are dipeptides, consisting of two amino acids joined by a single peptide bond. There are also tripeptides, tetrpeptides, etc. -Amino acids which have been inc ...
... -Peptides are distinguished from proteins on the basis of size, typically containing fewer than 50 monomer (AA) units. -The shortest peptides are dipeptides, consisting of two amino acids joined by a single peptide bond. There are also tripeptides, tetrpeptides, etc. -Amino acids which have been inc ...
Read about actions of formaldehyde on antigens
... can be detected only in unfixed cryosections; most are more easily detected after fixation. Paraffin embedding is usually preferable to either plastic embedding or cutting unembedded specimens with a vibrating microtome or in a cryostat. There are, however, plenty of exceptions to this generalizatio ...
... can be detected only in unfixed cryosections; most are more easily detected after fixation. Paraffin embedding is usually preferable to either plastic embedding or cutting unembedded specimens with a vibrating microtome or in a cryostat. There are, however, plenty of exceptions to this generalizatio ...
Alfred G. Gilman - Nobel Lecture
... implying that distinct receptors could interact with a common pool of adenylyl cyclase (4). The issue was resolved definitively in the 1970’s with the advent of ligand binding assays for receptors. Receptors could finally be examined by methods that did not rely on detection of a functional response ...
... implying that distinct receptors could interact with a common pool of adenylyl cyclase (4). The issue was resolved definitively in the 1970’s with the advent of ligand binding assays for receptors. Receptors could finally be examined by methods that did not rely on detection of a functional response ...
REVIEWS Structural insights into the function of the
... The relative tissue abundance of different isoforms is therefore an important factor in dictating which population of Rabs is associated with a particular GDI isoform. However, at least one isoform, the mouse GDI-2 (the ~/-isoform) is specifically enriched in endosomal compartments ]~ suggesting a m ...
... The relative tissue abundance of different isoforms is therefore an important factor in dictating which population of Rabs is associated with a particular GDI isoform. However, at least one isoform, the mouse GDI-2 (the ~/-isoform) is specifically enriched in endosomal compartments ]~ suggesting a m ...
ZO-3, a Novel Member of the MAGUK Protein Family Found at the
... boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. The molecular configuration of the tight junction has generated considerable interest in the last decade. Actin filaments (17, 29) and the peripheral membrane proteins ZO ...
... boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. The molecular configuration of the tight junction has generated considerable interest in the last decade. Actin filaments (17, 29) and the peripheral membrane proteins ZO ...
Effects of Macromolecular Crowding on Protein Folding
... Protein folding is the process whereby an extended and unstructured polypeptide is converted into a compact folded structure that typically constitutes its functional form. The process has been characterized extensively in-vitro in dilute buffer solutions over the last few decades. However, invivo, ...
... Protein folding is the process whereby an extended and unstructured polypeptide is converted into a compact folded structure that typically constitutes its functional form. The process has been characterized extensively in-vitro in dilute buffer solutions over the last few decades. However, invivo, ...
Apolipophorins and insects immune response
... 3’) located at one end of the α-helix bundle are present. They adapt an orientation nearly perpendicular to the long axis of the bundle (Wang et al., 1997; Narayanaswami et al., 1999; Fan et al., 2003; Sun et al., 2012). Because they are exposed to the solvent, it has been suggested that these short ...
... 3’) located at one end of the α-helix bundle are present. They adapt an orientation nearly perpendicular to the long axis of the bundle (Wang et al., 1997; Narayanaswami et al., 1999; Fan et al., 2003; Sun et al., 2012). Because they are exposed to the solvent, it has been suggested that these short ...
Characterization of the Enzymatic Component of the ADP
... Certain strains of Clostridium difficile produce the ADP-ribosyltransferase CDT, which is a binary actin ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component ...
... Certain strains of Clostridium difficile produce the ADP-ribosyltransferase CDT, which is a binary actin ADP-ribosylating toxin. The toxin consists of the binding component CDTb, which mediates receptor binding and cellular uptake, and the enzyme component CDTa. Here we studied the enzyme component ...
Transcription Factor Veracity: 1s GBF3 Responsible for ABA
... The G-box at position -214 is indicated by the solid-line box. The half only 5' truncated clones were recovered during the interacG-box at position -190 is indicated by the dotted-line box. The position screen, whereas full-length clones were recovered by tions of the nucleotides in the G-box are in ...
... The G-box at position -214 is indicated by the solid-line box. The half only 5' truncated clones were recovered during the interacG-box at position -190 is indicated by the dotted-line box. The position screen, whereas full-length clones were recovered by tions of the nucleotides in the G-box are in ...
Structural requirements of KTS-disintegrins for inhibition of α1β1
... disintegrin display concerted motions in the 100–300 ps timescale [29]. The shape and size of its short integrin-binding loop along with its composition, flexibility and the distinct orientation of the KTS tripeptide may underlie the structural basis of the unique selectivity and specificity of obtu ...
... disintegrin display concerted motions in the 100–300 ps timescale [29]. The shape and size of its short integrin-binding loop along with its composition, flexibility and the distinct orientation of the KTS tripeptide may underlie the structural basis of the unique selectivity and specificity of obtu ...
GLYCOLIPID ANCHORING OF CELL SURFACE PROTEINS Mark C
... variants.39,40 The CRD itself is associated with a complex oligosaccharide that was attached to the C-terminus of the VSG polypeptide.39,40,41,42 The linkage between the oligosaccharide and the C-terminal amino acid was shown to involve ethanolamine in amide linkage to the α-carboxyl group.43 In ord ...
... variants.39,40 The CRD itself is associated with a complex oligosaccharide that was attached to the C-terminus of the VSG polypeptide.39,40,41,42 The linkage between the oligosaccharide and the C-terminal amino acid was shown to involve ethanolamine in amide linkage to the α-carboxyl group.43 In ord ...
Product_Cheluminate_100915
... The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, ...
... The principle of the bicinchoninic acid (BCA) assay is similar to the Lowry procedure, in that both rely on the formation of a Cu2+-protein complex under alkaline condition followed by reduction of the Cu2+ to Cu1+. The amount of reduction is proportional to the protein present. In the second step, ...
thebacterialflagellum
... The dutch scientist Antonie van Leeuwenhoek was the first to describe bacteria in the late 17th century [1]. He manufactured microscopes and discovered small animals in his well water. He described them as “little eels, or worms, lying all huddled up together and wriggling” [2]. The bacteria he was ...
... The dutch scientist Antonie van Leeuwenhoek was the first to describe bacteria in the late 17th century [1]. He manufactured microscopes and discovered small animals in his well water. He described them as “little eels, or worms, lying all huddled up together and wriggling” [2]. The bacteria he was ...
The African Swine Fever Virus Proteins p54 and p30 Are
... specifically to these cells, and their binding was equally inhibited by neutralizing antibodies. Binding of 35S-labeled recombinant p54 and p30 proteins to macrophages was specifically competed by an excess of unlabeled p54 and p30, respectively. However, cross-binding inhibition was not observed, s ...
... specifically to these cells, and their binding was equally inhibited by neutralizing antibodies. Binding of 35S-labeled recombinant p54 and p30 proteins to macrophages was specifically competed by an excess of unlabeled p54 and p30, respectively. However, cross-binding inhibition was not observed, s ...
Membrane Topology of Cytochrome P450 2B4 in
... as well as to physiologically occurring compounds such as retinoids, steroids, and prostaglandins, has made this class of heme–monoxygenases the target of a wide range of structure–function investigations. Unfortunately, the vast majority of cytochromes P450 are membrane proteins that are not easily ...
... as well as to physiologically occurring compounds such as retinoids, steroids, and prostaglandins, has made this class of heme–monoxygenases the target of a wide range of structure–function investigations. Unfortunately, the vast majority of cytochromes P450 are membrane proteins that are not easily ...
Characterisation of Milk Proteins in Confectionery Products
... The proteins in milk, although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery a ...
... The proteins in milk, although representing only a small percentage (3-4 %) of its total composition are considered to possess unique structural and functional properties which will influence these final quality parameters as well as affecting processing. Of particular impor tance in confectionery a ...
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... synthesis at various times after the initiation of maturation. The addition of cycloheximide to extrafollicular oocytes during the first 4h after initiation invariably blocks nuclear breakdown (percentage of metaphase oocytes <5 % in all groups from 0-4 h). On the other hand over 50 % of oocytes und ...
... synthesis at various times after the initiation of maturation. The addition of cycloheximide to extrafollicular oocytes during the first 4h after initiation invariably blocks nuclear breakdown (percentage of metaphase oocytes <5 % in all groups from 0-4 h). On the other hand over 50 % of oocytes und ...
GTPase domains ofras p21 oncogene protein and elongation factor
... GTPase domains are functional and structural units employed as molecular switches in a variety of important cellular functions, such as growth control, protein biosynthesis, and membrane traffic. Amino acid sequences of more than 100 members of different subfamilies are known, but crystal structures ...
... GTPase domains are functional and structural units employed as molecular switches in a variety of important cellular functions, such as growth control, protein biosynthesis, and membrane traffic. Amino acid sequences of more than 100 members of different subfamilies are known, but crystal structures ...
A Point Mutation in the Ethylene-Inducing Xylanase Elicitor Inhibits
... Induction of Ethylene Biosynthesis by Different Xylanases The enzymatic activity (b-1-4-endoxylanase) of EIX was compared with the enzymatic activity of XynI and XynII isolated from Trichoderma reesei (Torronen et al., 1992). EIX and XynII had similar enzymatic activity, while XynI was less active ( ...
... Induction of Ethylene Biosynthesis by Different Xylanases The enzymatic activity (b-1-4-endoxylanase) of EIX was compared with the enzymatic activity of XynI and XynII isolated from Trichoderma reesei (Torronen et al., 1992). EIX and XynII had similar enzymatic activity, while XynI was less active ( ...
PAI-1 - Trace: Tennessee Research and Creative Exchange
... insertion of the N-terminal portion of the RCL into beta-sheet A during latency transition. Spectral isolation of the individual Trp sites should allow us to gain perspective on the metal binding conformational changes reported by each location. Therefore, studies were conducted on four Trp mutants, ...
... insertion of the N-terminal portion of the RCL into beta-sheet A during latency transition. Spectral isolation of the individual Trp sites should allow us to gain perspective on the metal binding conformational changes reported by each location. Therefore, studies were conducted on four Trp mutants, ...
The proposed channel-enzyme transient receptor potential
... The solubility and relative stability of mitochondrial NUDT9 and three of our chimeric constructs (Chi2, 3S, 4S) allowed purification of milligram amounts of these recombinant proteins to reasonable homogeneity (Figure 3—figure supplement 1) and assessment of their enzymatic activities. Furthermore, ...
... The solubility and relative stability of mitochondrial NUDT9 and three of our chimeric constructs (Chi2, 3S, 4S) allowed purification of milligram amounts of these recombinant proteins to reasonable homogeneity (Figure 3—figure supplement 1) and assessment of their enzymatic activities. Furthermore, ...
University of Groningen Cross-linking of dimeric CitS and GltS
... and includes the Ion Transporter (IT) superfamily ...
... and includes the Ion Transporter (IT) superfamily ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.