Bacterial tail anchors can target to the mitochondrial outer
... bioRxiv preprint first posted online Feb. 25, 2017; doi: http://dx.doi.org/10.1101/111716. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
... bioRxiv preprint first posted online Feb. 25, 2017; doi: http://dx.doi.org/10.1101/111716. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. All rights reserved. No reuse allowed without permission. ...
How do potentials derived from structural databases relate to true
... After all, the native proteins may not have been maximally optimized for stability through evolution because itis known that sometimes mutations introduced in the native proteins can yield more stable proteins (Lim et al., 1994). The unique stable fold of a protein is apparently an essential feature ...
... After all, the native proteins may not have been maximally optimized for stability through evolution because itis known that sometimes mutations introduced in the native proteins can yield more stable proteins (Lim et al., 1994). The unique stable fold of a protein is apparently an essential feature ...
Articulins and epiplasmins - Journal of Cell Science
... sequences of epiplasmins show that they are rich in valine and proline (Coffe et al., 1996), they do not display the characteristics of VPV-motifs, i.e. the alternation of valine and proline residues and the charge distribution typical of articulins. We have analysed the occurrence of articulins and ...
... sequences of epiplasmins show that they are rich in valine and proline (Coffe et al., 1996), they do not display the characteristics of VPV-motifs, i.e. the alternation of valine and proline residues and the charge distribution typical of articulins. We have analysed the occurrence of articulins and ...
Classification Scheme of Eukaryotic Transcription Factors
... as dimers (rnultimers), and the composition of these complexes mayaiso influence the DNA-binding speci ficity. For these reasons, the classification scheme outlined in Table I is mainly based on the properties of the DNA-binding domain, wherever appropriate in conjunction with the dimerization domai ...
... as dimers (rnultimers), and the composition of these complexes mayaiso influence the DNA-binding speci ficity. For these reasons, the classification scheme outlined in Table I is mainly based on the properties of the DNA-binding domain, wherever appropriate in conjunction with the dimerization domai ...
Zinc finger proteins
... wrap smoothly around the major groove like those of Zif268 In contrast, fingers 4–5–6 form an open, extended structure running along one side of the DNA. Of these, only finger 5 makes contacts with bases in the major groove. The flanking fingers, 4 and 6, appear to serve primarily as spacer elemen ...
... wrap smoothly around the major groove like those of Zif268 In contrast, fingers 4–5–6 form an open, extended structure running along one side of the DNA. Of these, only finger 5 makes contacts with bases in the major groove. The flanking fingers, 4 and 6, appear to serve primarily as spacer elemen ...
Ribosylation of bovine serum albumin induces ROS accumulation
... misfold and form amyloid fibrils in a stepwise process from prefibrils to fibrils (Necula and Kuret 2004). Glycation alters the biological activity of proteins and degradation mechanisms. Protein cross-linking by glycation results in the formation of detergent-insoluble and protease-resistant aggreg ...
... misfold and form amyloid fibrils in a stepwise process from prefibrils to fibrils (Necula and Kuret 2004). Glycation alters the biological activity of proteins and degradation mechanisms. Protein cross-linking by glycation results in the formation of detergent-insoluble and protease-resistant aggreg ...
Novel Ubiquitin Fusion Proteins: Ribosomal Protein
... present in B. natans polyubiquitin genes and to the ubiquitin moieties of the S27a and L40 fusion proteins. In contrast, the ubiquitin extension encoded by actin-3 was more divergent at the protein level than is typical of ubiquitin (the cDNA encoding this gene was slightly truncated at its 50 end). ...
... present in B. natans polyubiquitin genes and to the ubiquitin moieties of the S27a and L40 fusion proteins. In contrast, the ubiquitin extension encoded by actin-3 was more divergent at the protein level than is typical of ubiquitin (the cDNA encoding this gene was slightly truncated at its 50 end). ...
Protein Quality Control as a Strategy for Cellular Regulation
... conditions that demand its levels to be altered. Examples include the regulated degradation of p53,4 temporally programmed destruction of cyclins and other cell cycle regulators,5 and the selective degradation of glucose-synthesizing enzymes after feeding.6 In all cases, unique features of the targe ...
... conditions that demand its levels to be altered. Examples include the regulated degradation of p53,4 temporally programmed destruction of cyclins and other cell cycle regulators,5 and the selective degradation of glucose-synthesizing enzymes after feeding.6 In all cases, unique features of the targe ...
SEDS proteins are a widespread family of bacterial
... system modelled after those used to purify G-protein-coupled receptors, in which a SUMO–Flag–RodA fusion and the SUMO protease Ulp1 were co-expressed. SUMO cleavage generates an aminoterminal aspartic acid in the Flag tag that is recognized by the M1 anti-Flag monoclonal antibody, allowing for r ...
... system modelled after those used to purify G-protein-coupled receptors, in which a SUMO–Flag–RodA fusion and the SUMO protease Ulp1 were co-expressed. SUMO cleavage generates an aminoterminal aspartic acid in the Flag tag that is recognized by the M1 anti-Flag monoclonal antibody, allowing for r ...
PyMOL Modelling Workshop Outline of PyMOL usage
... atomic coordinates) as well as the coordinates for the atoms. When assessing a structure, one needs to take account of the resolution and R-factor, error estimates and missing residues. There is information about the sequence that was used to determine the structure with a sequence database referenc ...
... atomic coordinates) as well as the coordinates for the atoms. When assessing a structure, one needs to take account of the resolution and R-factor, error estimates and missing residues. There is information about the sequence that was used to determine the structure with a sequence database referenc ...
Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in
... Figure 2. MOM72 is responsible for specific binding of the ADP/ATP carrier to yeast mitocbondria. (.4 and B) Antibodies against yeast MOM72 inhibit specific binding of AAC. Isolated yeast mitochondria (10 #g of protein) were pre-incubated with IgGs prepared from preimmune serum or from antisera dire ...
... Figure 2. MOM72 is responsible for specific binding of the ADP/ATP carrier to yeast mitocbondria. (.4 and B) Antibodies against yeast MOM72 inhibit specific binding of AAC. Isolated yeast mitochondria (10 #g of protein) were pre-incubated with IgGs prepared from preimmune serum or from antisera dire ...
Identification of a Second Collagen-Like
... desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB. as demonstrated by monoclonal antibody labeling. The peptide backbo ...
... desorption/ionization time-of-flight mass spectrometry (MS) and the carbohydrate components by gas chromatography-mass spectrometry and tandem mass spectrometry. Spore-specific sugars were also demonstrated to be components of BclB. as demonstrated by monoclonal antibody labeling. The peptide backbo ...
G PROTEIN βγ SUBUNITS
... other proteins that make up the WD-repeat superfamily. Members of this family do not have an immediately obvious common function; they are involved in diverse cellular pathways such as signal transduction, pre-mRNA splicing, transcriptional regulation, assembly of the cytoskeleton, and vesicular tra ...
... other proteins that make up the WD-repeat superfamily. Members of this family do not have an immediately obvious common function; they are involved in diverse cellular pathways such as signal transduction, pre-mRNA splicing, transcriptional regulation, assembly of the cytoskeleton, and vesicular tra ...
- Scholarworks @ CSU San Marcos
... predict biomineralization proteins in Emiliania huxleyi. The K-NN predicted 86 proteins, the RBF-SVM predicted 3,441 proteins, and the L-SVM predict 11,671 proteins as possible biomineralization proteins For the E. huxleyi dataset, with 59 shared among all three algorithms, which suggests K-NN might ...
... predict biomineralization proteins in Emiliania huxleyi. The K-NN predicted 86 proteins, the RBF-SVM predicted 3,441 proteins, and the L-SVM predict 11,671 proteins as possible biomineralization proteins For the E. huxleyi dataset, with 59 shared among all three algorithms, which suggests K-NN might ...
F-actin Sequesters Elongation Factor from Interaction with
... let from F-actin to aa-tRNA as pH increases. The ability of EF-let to bind either F-actin or aa-tRNA in competition binding experiments is also consistent with the observation that EF-let's binding to F-actin and aatRNA is mutually exclusive. Two pH-sensitive actinbinding sequences in EF-let are ide ...
... let from F-actin to aa-tRNA as pH increases. The ability of EF-let to bind either F-actin or aa-tRNA in competition binding experiments is also consistent with the observation that EF-let's binding to F-actin and aatRNA is mutually exclusive. Two pH-sensitive actinbinding sequences in EF-let are ide ...
GFP (Green fluorescent protein)
... • Someone might be saying – why?who cares about this little green protein from a jellyfish? But it turns out with amazingly useful in scientific research, because it allows us to look directly into the inner workings of cells. It is easy to find out where GFP is at any given time: you just have to s ...
... • Someone might be saying – why?who cares about this little green protein from a jellyfish? But it turns out with amazingly useful in scientific research, because it allows us to look directly into the inner workings of cells. It is easy to find out where GFP is at any given time: you just have to s ...
Systematic Structure-Function Analysis of the Small GTPase Arf1 in Yeast.
... Harter, 1999). For Arf proteins, the coupling of protein binding to membrane association is thought to occur via extrusion of the unique myristoylated N terminus upon GTP binding (Antonny et al., 1997). In this study we aimed to determine the regions of the yeast protein Arf1 that are important for ...
... Harter, 1999). For Arf proteins, the coupling of protein binding to membrane association is thought to occur via extrusion of the unique myristoylated N terminus upon GTP binding (Antonny et al., 1997). In this study we aimed to determine the regions of the yeast protein Arf1 that are important for ...
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... It is produced from whole raw corn, and not from corn processing by-products. Zein has traditionally been isolated and manufactured from corn gluten meal, a coproduct of the corn wet milling industry which has been exposed to steeping chemicals such as sulfur dioxide and lactic acid, among others, a ...
... It is produced from whole raw corn, and not from corn processing by-products. Zein has traditionally been isolated and manufactured from corn gluten meal, a coproduct of the corn wet milling industry which has been exposed to steeping chemicals such as sulfur dioxide and lactic acid, among others, a ...
A Positive Selection Function for miRNA
... The combinations are then translated For example, miRNA let-7 (6) in the 5′ to 3′ has one open reading frame and three open reading frames in its antiparallel complement in the 5′ to 3′ direction. The antiparallel complement would correspond to coding sequences which would appear in mRNA which are ...
... The combinations are then translated For example, miRNA let-7 (6) in the 5′ to 3′ has one open reading frame and three open reading frames in its antiparallel complement in the 5′ to 3′ direction. The antiparallel complement would correspond to coding sequences which would appear in mRNA which are ...
Protein S-nitrosylation in photosynthetic organisms: A
... that have no unpaired electrons, but it tends to combine with other radicals and transition metals [34]. NO is also a small and lipophilic molecule that can easily cross lipid membranes and, thanks to its limited reactivity, is considered relatively long living in vivo [35]. With a half life of up t ...
... that have no unpaired electrons, but it tends to combine with other radicals and transition metals [34]. NO is also a small and lipophilic molecule that can easily cross lipid membranes and, thanks to its limited reactivity, is considered relatively long living in vivo [35]. With a half life of up t ...
Diversity of heterotrimeric G-protein γ subunits in plants | SpringerLink
... different plant taxa were translated into protein sequences and used as queries in additional BLAST searches. In total, over 300 non-redundant sequences representing plant Gγ subunits from land plants were obtained with more than one subunit found in most species. After discarding short and incomple ...
... different plant taxa were translated into protein sequences and used as queries in additional BLAST searches. In total, over 300 non-redundant sequences representing plant Gγ subunits from land plants were obtained with more than one subunit found in most species. After discarding short and incomple ...
Slides
... family (Structural classification of Proteins). 2) Prediction of an Enzyme class given other enzymes that have a similar function on other substrates. ...
... family (Structural classification of Proteins). 2) Prediction of an Enzyme class given other enzymes that have a similar function on other substrates. ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.