2-D Quant Kit - GE Healthcare Life Sciences
... The kit contains reagents sufficient for 500 data points. Warning For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals. ...
... The kit contains reagents sufficient for 500 data points. Warning For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals. ...
Universal strategies in research and drug discovery based on protein
... and Renilla luciferases has been demonstrated41,45. It is known that PCAs that are based on GFP and variants are irreversible, which can be useful (for example, for trapping and visualizing rare or transient complexes), but interpretation of the kinetics of complex turnover or localization of intera ...
... and Renilla luciferases has been demonstrated41,45. It is known that PCAs that are based on GFP and variants are irreversible, which can be useful (for example, for trapping and visualizing rare or transient complexes), but interpretation of the kinetics of complex turnover or localization of intera ...
Microtubules
... as well as other proteins, are either present in centrioles or required for their formation. ...
... as well as other proteins, are either present in centrioles or required for their formation. ...
Autophagy in Plasmodium, a multifunctional pathway?
... The genome sequence of P. falciparum was published in 2002 and is stored along with various data associated with each gene in the Plasmodium Genome database (PlasmoDB) [47,48]. The amino acid coding sequence of Saccharomyces cerevisiae and Homo sapiens ATG genes were queried for putative homologues ...
... The genome sequence of P. falciparum was published in 2002 and is stored along with various data associated with each gene in the Plasmodium Genome database (PlasmoDB) [47,48]. The amino acid coding sequence of Saccharomyces cerevisiae and Homo sapiens ATG genes were queried for putative homologues ...
embor2011116-sup-0001
... It uses the Dubay et al. algorithm, but considers only the intrinsic factors (charge, hydrophobicity and PATs) to calculate the amyloid aggregation propensities for all the naturally occurring amino acid residues. While the algorithm scans a polypeptide sequence, it assigns to each residue i the ave ...
... It uses the Dubay et al. algorithm, but considers only the intrinsic factors (charge, hydrophobicity and PATs) to calculate the amyloid aggregation propensities for all the naturally occurring amino acid residues. While the algorithm scans a polypeptide sequence, it assigns to each residue i the ave ...
Gene Section NOL3 (nucleolar protein 3 (apoptosis repressor with CARD domain))
... pathway by interfering with DISC (Death Inducing Signaling Complex) formation. This is accomplished by the direct interaction of the ARC CARD with the death domains (DD) of Fas and FADD, and with the death effector domain (DED) of procaspase-8. These death-fold interactions are novel in that they ar ...
... pathway by interfering with DISC (Death Inducing Signaling Complex) formation. This is accomplished by the direct interaction of the ARC CARD with the death domains (DD) of Fas and FADD, and with the death effector domain (DED) of procaspase-8. These death-fold interactions are novel in that they ar ...
TPN-III-No acute stress
... Her malnutrition occurred over several months and repletion may take equally as long. ...
... Her malnutrition occurred over several months and repletion may take equally as long. ...
Protein expression by a Beijing strain differs from that of another
... Quantification of ICD in culture supernatants showed no significant difference in the extent of autolysis in the three strains (data not shown). The protein expression profiles were highly similar between the strains, enabling visual comparison. Spot differences consistent between quadruplicate gels ...
... Quantification of ICD in culture supernatants showed no significant difference in the extent of autolysis in the three strains (data not shown). The protein expression profiles were highly similar between the strains, enabling visual comparison. Spot differences consistent between quadruplicate gels ...
Tubulin folding is altered by mutations in a putative GTP binding motif
... 1992; Fontalba et al., 1993, 1995; Campo et al., 1994; Fig. 1). Soon after tubulin is synthesized it is bound to a TCP-1-containing chaperone particle (C900). Pulse-chase experiments have shown that, in the presence of nucleotides tubulin dimers and release factors, the disappearance of C900 complex ...
... 1992; Fontalba et al., 1993, 1995; Campo et al., 1994; Fig. 1). Soon after tubulin is synthesized it is bound to a TCP-1-containing chaperone particle (C900). Pulse-chase experiments have shown that, in the presence of nucleotides tubulin dimers and release factors, the disappearance of C900 complex ...
of Pseudomonas aeruginosa - BMC Microbiology
... Background: Community and nosocomial infections by Pseudomonas aeruginosa still create a major therapeutic challenge. The resistance of this opportunist pathogen to β-lactam antibiotics is determined mainly by production of the inactivating enzyme AmpC, a class C cephalosporinase with a regulation s ...
... Background: Community and nosocomial infections by Pseudomonas aeruginosa still create a major therapeutic challenge. The resistance of this opportunist pathogen to β-lactam antibiotics is determined mainly by production of the inactivating enzyme AmpC, a class C cephalosporinase with a regulation s ...
Prediction of protease substrates using sequence
... 2009), and only good quality models [those predicted to have >80% of their C-α atoms within 3.5 Å of the native state, as assessed by the model evaluation algorithm TSVMod (Eramian et al., 2008)] were considered (Fig. 1, step 2b). It has been previously shown that secondary structure features comput ...
... 2009), and only good quality models [those predicted to have >80% of their C-α atoms within 3.5 Å of the native state, as assessed by the model evaluation algorithm TSVMod (Eramian et al., 2008)] were considered (Fig. 1, step 2b). It has been previously shown that secondary structure features comput ...
INVESTIGATION INTO THE ALLOSTERIC REGULATION OF MITOTIC KINESIN EG5 Introduction Results
... involved in allosteric communication between the L5 loop and the other two sites (ATP-, & MT-binding sites) may also be conserved across other kinesin family members. Conserved site-to-site communication is therapeutically important. Allostery is a key consideration in drug design. We suspect effect ...
... involved in allosteric communication between the L5 loop and the other two sites (ATP-, & MT-binding sites) may also be conserved across other kinesin family members. Conserved site-to-site communication is therapeutically important. Allostery is a key consideration in drug design. We suspect effect ...
THE RDOA-DEPENDENT PHOSPHOPROTEOME PROFILE OF Salmonella enterica
... mutants suggested that RdoA kinase activity affects a wide range of cell functions, which could be the result of both direct and indirect phosphorylation of targets. In a search for RdoA’s target(s), the phosphoproteome profile of wild-type and rdoA null S. enterica was examined through phosphoprote ...
... mutants suggested that RdoA kinase activity affects a wide range of cell functions, which could be the result of both direct and indirect phosphorylation of targets. In a search for RdoA’s target(s), the phosphoproteome profile of wild-type and rdoA null S. enterica was examined through phosphoprote ...
spectroscopic studies of mosquito iridescent virus, its capsid
... acid inside the virion (RNA or DNA), and the concentration of virions in the environment or in the tissues of an infected animal. In particular, the fluorescence may be a novel tool to detect viruses and to monitor a viral infection of cells and may be part of a biodefense application [2]. Different ...
... acid inside the virion (RNA or DNA), and the concentration of virions in the environment or in the tissues of an infected animal. In particular, the fluorescence may be a novel tool to detect viruses and to monitor a viral infection of cells and may be part of a biodefense application [2]. Different ...
PRIONS THE INFECTIOUS PROTEINS
... Inhibition of ubiquitin-proteasome system(UPS). Deposition of aggresomes of PrPSc in nerve cells. Induction of Apoptosis with activation of Caspase 3 and Caspase 8. ...
... Inhibition of ubiquitin-proteasome system(UPS). Deposition of aggresomes of PrPSc in nerve cells. Induction of Apoptosis with activation of Caspase 3 and Caspase 8. ...
Interactions of liposomes and lipid-based carrier systems with blood
... PB values have turned out to be very useful in assessing the relation between the surface adsorption properties and the clearance behavior of liposomes. Our studies to date indicate that there is an inverse relationship between PB values and the circulation half-lives of liposomes. In particular, li ...
... PB values have turned out to be very useful in assessing the relation between the surface adsorption properties and the clearance behavior of liposomes. Our studies to date indicate that there is an inverse relationship between PB values and the circulation half-lives of liposomes. In particular, li ...
PDF + SI - GenScript
... complex exists, we embarked on independent purification methods. We prepared 0- to 12-h embryo extracts from Drosophila expressing a functional, FLAG-tagged version of Mcm6 (32). A single-step affinity purification of FLAG-Mcm6 from these flies captures mainly FLAG-Mcm6 in complex with Mcm2, Mcm4, a ...
... complex exists, we embarked on independent purification methods. We prepared 0- to 12-h embryo extracts from Drosophila expressing a functional, FLAG-tagged version of Mcm6 (32). A single-step affinity purification of FLAG-Mcm6 from these flies captures mainly FLAG-Mcm6 in complex with Mcm2, Mcm4, a ...
Proteases and proteolytic cleavage of storage
... A third group of constraints which have most certainly played a role in the evolution of these proteins, as well as the proteases that degrade them, are those imposed by storage protein reactivation. The storage proteins must be made susceptible to proteolytic attack by appropriate structural change ...
... A third group of constraints which have most certainly played a role in the evolution of these proteins, as well as the proteases that degrade them, are those imposed by storage protein reactivation. The storage proteins must be made susceptible to proteolytic attack by appropriate structural change ...
Introduction to Database Searching using MASCOT
... One of the strengths of PMF is that it is an easy experiment that can be performed using just about any mass spectrometer. The whole process is readily automated and MALDI instruments, in particular, can churn out high accuracy PMF data at a very high rate. In principal, it is a sensitive technique ...
... One of the strengths of PMF is that it is an easy experiment that can be performed using just about any mass spectrometer. The whole process is readily automated and MALDI instruments, in particular, can churn out high accuracy PMF data at a very high rate. In principal, it is a sensitive technique ...
MEMBRANE PROTEINS SYNTHESIZED BY
... the isolated enzyme forms stable interactions with high affinity for a limited number of sites on the cytoplasmic face of the membrane (21). Rabbit reticulocytes contain, with possibly one or two exceptions (22), the same proteins in their membranes as do rabbit erythrocytes. We recently showed that ...
... the isolated enzyme forms stable interactions with high affinity for a limited number of sites on the cytoplasmic face of the membrane (21). Rabbit reticulocytes contain, with possibly one or two exceptions (22), the same proteins in their membranes as do rabbit erythrocytes. We recently showed that ...
TD12 Characterization of DnaJ substrate specificity Reference
... G/E domain- contributes to stimulation of ATP hydrolysis (of DnaK) Zn binding site, amino acid ~144 C-terminal domain- amino acids 144-376, binds protein substrates Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers subs ...
... G/E domain- contributes to stimulation of ATP hydrolysis (of DnaK) Zn binding site, amino acid ~144 C-terminal domain- amino acids 144-376, binds protein substrates Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers subs ...
Functional analysis of the human CDC5L complex
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
... antibody and the protein revealed using ECL (Amersham). Lanes 1 and 2 are duplicates containing supernatants of mock immunodepletions using pre-immune IgG. Lane 3 contains the supernatant from antiCDC5L antibody immunodepleted nuclear extract and lane 4 is similar to lane 3 except that the antibody ...
Translocation of proteins across archaeal cytoplasmic membranes
... signal recognition particle (SRP), which plays an integral role in the targeting of co-translationally translocated proteins, are present in organisms of all domains of life, and have been well-characterized in bacteria and eukaryotes [12,13]. In addition to the universally conserved Sec components, ...
... signal recognition particle (SRP), which plays an integral role in the targeting of co-translationally translocated proteins, are present in organisms of all domains of life, and have been well-characterized in bacteria and eukaryotes [12,13]. In addition to the universally conserved Sec components, ...
CONFERENCE REPORT FROM A NOBEL MINISYMPOSIUM/JIM
... their native structures into amyloid fibrils, and in line with those observations almost all proteins apparently contain segments with the potential to form amyloid-like fibrils. However, only a tiny fraction of all proteins really forms amyloid - so far only 30 amyloid diseases have been described, ...
... their native structures into amyloid fibrils, and in line with those observations almost all proteins apparently contain segments with the potential to form amyloid-like fibrils. However, only a tiny fraction of all proteins really forms amyloid - so far only 30 amyloid diseases have been described, ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.