Protein quality control and elimination of protein waste: The role of
... functions are manifold: signaling, movement, transport, membrane fusion, cell protection, regulation or catalysis are only some of them [1]. The three-dimensional structure that proteins acquire after ribosomal synthesis of their amino acid chain is crucial to their function. This function has to be ...
... functions are manifold: signaling, movement, transport, membrane fusion, cell protection, regulation or catalysis are only some of them [1]. The three-dimensional structure that proteins acquire after ribosomal synthesis of their amino acid chain is crucial to their function. This function has to be ...
Not Every Disulfide Lasts Forever: Disulfide Bond
... dual regulation modes: on the transcriptional level, the Hsp33 gene (hslO) is under heat shock control (55), and on the posttranslational level, the Hsp33 protein is under oxidative stress control (30). Functional studies revealed that Hsp33 is a very potent molecular chaperone when oxidized. In thi ...
... dual regulation modes: on the transcriptional level, the Hsp33 gene (hslO) is under heat shock control (55), and on the posttranslational level, the Hsp33 protein is under oxidative stress control (30). Functional studies revealed that Hsp33 is a very potent molecular chaperone when oxidized. In thi ...
Protein Arginine Methylation in Candida albicans: Role
... Whereas the roles of arginine methylation are diverse, many roles are linked to modulation of protein-protein interactions ...
... Whereas the roles of arginine methylation are diverse, many roles are linked to modulation of protein-protein interactions ...
Heterotrimeric G Protein–Coupled Signaling in Plants
... However, what we have learned and taught is but one version of G signaling, a version influenced by the enormous anthropocentric focus on human health and disease. Can plants tell us something new about G signaling? In the past 10 years, research on plant G proteins has revealed a fundamental differe ...
... However, what we have learned and taught is but one version of G signaling, a version influenced by the enormous anthropocentric focus on human health and disease. Can plants tell us something new about G signaling? In the past 10 years, research on plant G proteins has revealed a fundamental differe ...
Proteomics of Plasma Membranes from Poplar Trees Reveals
... Published, MCP Papers in Press, November 28, 2009, DOI 10.1074/mcp.M900289-MCP200 ...
... Published, MCP Papers in Press, November 28, 2009, DOI 10.1074/mcp.M900289-MCP200 ...
Role of N-linked oligosaccharide chains in the processing and
... different carbohydrate structure from those of the other three glycosylation sites and this varied glycosylation was responsible for the appearance of two forms of the H protein. The functional glycosylation sites were ...
... different carbohydrate structure from those of the other three glycosylation sites and this varied glycosylation was responsible for the appearance of two forms of the H protein. The functional glycosylation sites were ...
Kinesin superfamily proteins and their various functions and dynamics
... determined by in vitro motility assays, is mostly approximately 0.1–1.5 Am s 1, which seems to closely correlate with the velocity observed in vivo for each KIF. Although the motor domain is highly conserved among different KIFs, regions other than the motor domain are quite divergent and these regi ...
... determined by in vitro motility assays, is mostly approximately 0.1–1.5 Am s 1, which seems to closely correlate with the velocity observed in vivo for each KIF. Although the motor domain is highly conserved among different KIFs, regions other than the motor domain are quite divergent and these regi ...
Lysosomes and lysosomal disorders
... Lysosomal membrane contains more than 100 proteins, majority of which have unknown function. Proteins with known function include receptors, molecules participating in vesicular transport, transporters of small molecules, vacuolar ATPase etc. Oligosaccharide chains at the inner face of lysosomal me ...
... Lysosomal membrane contains more than 100 proteins, majority of which have unknown function. Proteins with known function include receptors, molecules participating in vesicular transport, transporters of small molecules, vacuolar ATPase etc. Oligosaccharide chains at the inner face of lysosomal me ...
生物核磁共振波谱学NMR in Biological Science 闫永彬博士Yong
... - W. R. Croasmun, W. M. K. Carlson, ed. "Two-Dimensional NMR Spectroscopy; Applications for Chemists and Biochemists", VCH 1994, ISBN 1-56081-664-3 (QD/96/N8/T87/1987). (mixture of introductory and application chapters, on heteronuclear spectroscopy etc., ...
... - W. R. Croasmun, W. M. K. Carlson, ed. "Two-Dimensional NMR Spectroscopy; Applications for Chemists and Biochemists", VCH 1994, ISBN 1-56081-664-3 (QD/96/N8/T87/1987). (mixture of introductory and application chapters, on heteronuclear spectroscopy etc., ...
Antioxidative Activities of Hydrolysates from Duck Egg White Using
... tenderizing meat and other proteins by cleaving peptide bonds of basic amino acids. It can degrade large molecules of proteins into small fragments. Lee and Chen (2002) hydrolyzed chicken egg white using papain and obtained several functional properties such as solubility, foaming capacity and cake ...
... tenderizing meat and other proteins by cleaving peptide bonds of basic amino acids. It can degrade large molecules of proteins into small fragments. Lee and Chen (2002) hydrolyzed chicken egg white using papain and obtained several functional properties such as solubility, foaming capacity and cake ...
Facing extremes: archaeal surface-layer (glyco)proteins
... glycosylation, suggesting that motifs apart from the consensus Asn-Xaa-Ser/Thr sequence are recognized by the haloarchaeal glycosylation machinery (Zeitler et al., 1998). Finally, several studies addressing S-layer glycoprotein glycosylation suggest that in archaea, protein glycosylation occurs on t ...
... glycosylation, suggesting that motifs apart from the consensus Asn-Xaa-Ser/Thr sequence are recognized by the haloarchaeal glycosylation machinery (Zeitler et al., 1998). Finally, several studies addressing S-layer glycoprotein glycosylation suggest that in archaea, protein glycosylation occurs on t ...
Searching algorithm for type IV secretion system effectors 1.0: a tool
... alternative to machine learning approach for less-studied pathogenic bacteria. In this section, we describe the algorithm used by S4TE, how the parameters of this software were estimated from the literature and how S4TE performs on different genomes. The essential features of the S4TE program, as de ...
... alternative to machine learning approach for less-studied pathogenic bacteria. In this section, we describe the algorithm used by S4TE, how the parameters of this software were estimated from the literature and how S4TE performs on different genomes. The essential features of the S4TE program, as de ...
Branched-Chain Amino Acid Supplementation: A Critical Analysis of
... structure of their residual side chain. BCAAs play a crucial role in many biochemical processes essential to human life. They represent three of nine amino acids that cannot be synthesized in the human body. For this reason BCAAs are categorized as essential amino acids because their requirements mu ...
... structure of their residual side chain. BCAAs play a crucial role in many biochemical processes essential to human life. They represent three of nine amino acids that cannot be synthesized in the human body. For this reason BCAAs are categorized as essential amino acids because their requirements mu ...
Reivew, Hemoglobin
... HEMOGLOBIN As deoxygenated hemoglobin becomes oxygenated, significant structural changes take place the proximal hisitidine and its helix shift one heterodimer rotates and slides relative to the other existing noncovalent bonds are broken and replaced by new ...
... HEMOGLOBIN As deoxygenated hemoglobin becomes oxygenated, significant structural changes take place the proximal hisitidine and its helix shift one heterodimer rotates and slides relative to the other existing noncovalent bonds are broken and replaced by new ...
The twin arginine protein transport pathway exports multiple
... TATFIND 1.4 and TatP have been shown to have bona fide Tat-targeting signals (Widdick et al., 2006). This therefore gives confidence that the subset of proteins predicted to be Tat substrates by both programmes are highly likely to represent real substrates. For S. scabies, 82 proteins are predicted ...
... TATFIND 1.4 and TatP have been shown to have bona fide Tat-targeting signals (Widdick et al., 2006). This therefore gives confidence that the subset of proteins predicted to be Tat substrates by both programmes are highly likely to represent real substrates. For S. scabies, 82 proteins are predicted ...
The Evolution of SMC Proteins: Phylogenetic Analysis and Structural
... activity appears to be required for the full function of SMC-containing complexes, as shown by mutagenesis of the ATP-binding domain (Chuang, Albertson, and Meyer 1994; Verkade et al. 1999; Fousteri and Lehmann 2000; Hirano et al. 2001) or the use of nonhydrolysable ATP analogs (Kimura and Hirano 19 ...
... activity appears to be required for the full function of SMC-containing complexes, as shown by mutagenesis of the ATP-binding domain (Chuang, Albertson, and Meyer 1994; Verkade et al. 1999; Fousteri and Lehmann 2000; Hirano et al. 2001) or the use of nonhydrolysable ATP analogs (Kimura and Hirano 19 ...
28. biosynthesis of proteins
... mRNA are complementary. Transcription may also be defined as DNA-dependent RNA synthesis. The third step is translation, in which the genetic message coded in mRNA is translated, on the ribosomes, into a protein with a specific sequence of amino acids. In other words, reproduction of a primary polyp ...
... mRNA are complementary. Transcription may also be defined as DNA-dependent RNA synthesis. The third step is translation, in which the genetic message coded in mRNA is translated, on the ribosomes, into a protein with a specific sequence of amino acids. In other words, reproduction of a primary polyp ...
Cell-Free (In Vitro) Protein Expression
... Western blotting detection using primary antibody to the expressed protein ...
... Western blotting detection using primary antibody to the expressed protein ...
Hormone actions
... • Interaction of hormone with its receptor – Generation of intracellular signals – Different among different hormones • Production of chemicals within the cell – Secondary messenger ...
... • Interaction of hormone with its receptor – Generation of intracellular signals – Different among different hormones • Production of chemicals within the cell – Secondary messenger ...
Mitochondrial Proton Leak and the Uncoupling Proteins
... potential may indeed result from an augmentation of the leak pathway (or ATP turnover). An important concern with the yeast model used to measure uncoupling of respiration is the presence, in the line of yeast (laboratory strain W303) used in many of the above studies, of an ATP-inducible proton con ...
... potential may indeed result from an augmentation of the leak pathway (or ATP turnover). An important concern with the yeast model used to measure uncoupling of respiration is the presence, in the line of yeast (laboratory strain W303) used in many of the above studies, of an ATP-inducible proton con ...
Contribution of Fermentation Yeast to Final Amino Acid Profile in
... DDGS proteins come from corn and yeast. Yet, the effect of fermentation yeast on DDGS protein quantity and quality (AA profile) has not been well documented. Based on literature review, there are at least 4 methods that have been described to estimate yeast contribution in distillers grains products ...
... DDGS proteins come from corn and yeast. Yet, the effect of fermentation yeast on DDGS protein quantity and quality (AA profile) has not been well documented. Based on literature review, there are at least 4 methods that have been described to estimate yeast contribution in distillers grains products ...
ESCRT requirements for EIAV budding | SpringerLink
... Vps60p/CHMP5 [43,44] and Vps4p/VPS4A/B, and stimulates enzyme assembly and ATPase activity [45-48]; and the ESCRT-III adaptor protein, Bro1p/ALIX, which binds and stabilizes the Snf7p/CHMP4A/B/C filaments, and recruits the deubiquitinating enzyme, Doa4p/UBPY [23,49]. Although the core yeast ESCRT co ...
... Vps60p/CHMP5 [43,44] and Vps4p/VPS4A/B, and stimulates enzyme assembly and ATPase activity [45-48]; and the ESCRT-III adaptor protein, Bro1p/ALIX, which binds and stabilizes the Snf7p/CHMP4A/B/C filaments, and recruits the deubiquitinating enzyme, Doa4p/UBPY [23,49]. Although the core yeast ESCRT co ...
Sensory control of longevity by the ODR-3 and GPA
... The genome of nematode Caenorhabditis elegans encodes 21 Gα, 2 Gβ and 2 Gγ subunits3,4. Each of the mammalian classes of Gα subunits is represented by one member in C. elegans: gsa-1 for Gs, goa-1 for Gi/o, egl-30 for Gq and gpa-12 for G12. The conserved Gα subunits, the two Gβ subunits and one Gγ, ...
... The genome of nematode Caenorhabditis elegans encodes 21 Gα, 2 Gβ and 2 Gγ subunits3,4. Each of the mammalian classes of Gα subunits is represented by one member in C. elegans: gsa-1 for Gs, goa-1 for Gi/o, egl-30 for Gq and gpa-12 for G12. The conserved Gα subunits, the two Gβ subunits and one Gγ, ...
NHS ester - BroadPharm
... the most important thing. Avoid using buffers containing amines (Tris can sometimes be used but not recommended). When doing large-scale labeling (hundreds of milligrams of NHS ester), note that the mixture tends to acidify with time because of hydrolysis of NHS ester. Monitor pH, or use more concen ...
... the most important thing. Avoid using buffers containing amines (Tris can sometimes be used but not recommended). When doing large-scale labeling (hundreds of milligrams of NHS ester), note that the mixture tends to acidify with time because of hydrolysis of NHS ester. Monitor pH, or use more concen ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.