Ternary complex structure of Aristaless and Clawless bound to DNA
... on the degree of conservation of the amino acid sequence of each protein. Al and its homologues share high sequence similarity in two regions: the homeodomain (85 - 144 of Al) (Figure 1A) and the OAR domain (376 - 391 of Al). The OAR domain is thought to negatively regulate the DNA-binding affinity ...
... on the degree of conservation of the amino acid sequence of each protein. Al and its homologues share high sequence similarity in two regions: the homeodomain (85 - 144 of Al) (Figure 1A) and the OAR domain (376 - 391 of Al). The OAR domain is thought to negatively regulate the DNA-binding affinity ...
Mercury, Cadmium, and Arsenite Enhance Heat Shock Protein
... BACKGROUND: Cells respond to adverse environmental stimuli by enhancing the expression of specific genes, the products of which include a suite of proteins known as heat shock proteins (hsps), a response often attributed to cellular protection. METHODS: In this study, we characterized alterations in ...
... BACKGROUND: Cells respond to adverse environmental stimuli by enhancing the expression of specific genes, the products of which include a suite of proteins known as heat shock proteins (hsps), a response often attributed to cellular protection. METHODS: In this study, we characterized alterations in ...
PROTEIN METABOLISM
... generate any polypeptide in this experiment because it spontaneously forms tetraplexes (see Fig. 8–22) that cannot be bound by ribosomes. The synthetic polynucleotides used in such experiments were prepared with polynucleotide phosphorylase (p. 1020), which catalyzes the formation of RNA polymers st ...
... generate any polypeptide in this experiment because it spontaneously forms tetraplexes (see Fig. 8–22) that cannot be bound by ribosomes. The synthetic polynucleotides used in such experiments were prepared with polynucleotide phosphorylase (p. 1020), which catalyzes the formation of RNA polymers st ...
Amino Acids, Peptides and Proteins in Organic Chemistry: Analysis
... In an MS experiment, the intensity of the signal as the peptide elutes for fragmentation in every run8. Therefore, a critical requirement is from the chromatographic column can be plotted over time (Fig. 1). the ability to find and quantify the peptide in different runs, even if The area under this ...
... In an MS experiment, the intensity of the signal as the peptide elutes for fragmentation in every run8. Therefore, a critical requirement is from the chromatographic column can be plotted over time (Fig. 1). the ability to find and quantify the peptide in different runs, even if The area under this ...
Evidence for Amino Acid Snorkeling from a High
... ABSTRACT Proteins localized to mitochondria by a carboxyl-terminal tail anchor (TA) play roles in apoptosis, mitochondrial dynamics, and mitochondrial protein import. To reveal characteristics of TAs that may be important for mitochondrial targeting, we focused our attention upon the TA of the Sacch ...
... ABSTRACT Proteins localized to mitochondria by a carboxyl-terminal tail anchor (TA) play roles in apoptosis, mitochondrial dynamics, and mitochondrial protein import. To reveal characteristics of TAs that may be important for mitochondrial targeting, we focused our attention upon the TA of the Sacch ...
ProRule: a new database containing functional
... physico-chemical properties associated with them, the proper reaction cannot take place and the function or structure will be altered. The fact that profiles generally cover domains or proteins over their entire length makes the identification of such biologically relevant residues less obvious than ...
... physico-chemical properties associated with them, the proper reaction cannot take place and the function or structure will be altered. The fact that profiles generally cover domains or proteins over their entire length makes the identification of such biologically relevant residues less obvious than ...
Bioinformatic Software in Web
... clones which are not seen with conventional techniques. Monitoring disease processes and protein expression. Discovering new disease markers and/or patterns in body fluids, cells, or tissues. ...
... clones which are not seen with conventional techniques. Monitoring disease processes and protein expression. Discovering new disease markers and/or patterns in body fluids, cells, or tissues. ...
The nutrient requirements of calves
... meet the requirements of the body. These metabolic functions include such things as synthesis of enzymes and hormones, cell division and cell repair, and so require a continuous supply of different types of protein and energy. The building blocks of all proteins are amino acids. There are more than ...
... meet the requirements of the body. These metabolic functions include such things as synthesis of enzymes and hormones, cell division and cell repair, and so require a continuous supply of different types of protein and energy. The building blocks of all proteins are amino acids. There are more than ...
The Serum Proteins of the Rat During Development
... An asymmetry was found in the trailing region of the gamma-globulin fraction during all stages of development, including the adult. An asymmetry very similar in appearance and position was also found by Gurvich & Karsaevskaya (1956), but only in sera from perinatal animals. These workers have design ...
... An asymmetry was found in the trailing region of the gamma-globulin fraction during all stages of development, including the adult. An asymmetry very similar in appearance and position was also found by Gurvich & Karsaevskaya (1956), but only in sera from perinatal animals. These workers have design ...
ppt - Chair of Computational Biology
... a, A backbone ribbon representation of the Itk cis SH2 domain with the Itk polyproline peptide (KPLPPTP shown in white) superimposed on the structure. The polyproline peptide residues are labeled using the one letter amino acid code and are numbered consecutively. In previously determined peptide–SH ...
... a, A backbone ribbon representation of the Itk cis SH2 domain with the Itk polyproline peptide (KPLPPTP shown in white) superimposed on the structure. The polyproline peptide residues are labeled using the one letter amino acid code and are numbered consecutively. In previously determined peptide–SH ...
Delivery of a Secreted Soluble Protein to the Vacuole via a
... not to have plant vacuolar targeting information. When yeast invertase is equipped with a plant signal peptide, catalytically active protein is secreted in the apoplast (von Schaewen et al., 1990; Dickinson et al., 1991). We chose the transmembrane domain of yeast calnexin because we assumed that th ...
... not to have plant vacuolar targeting information. When yeast invertase is equipped with a plant signal peptide, catalytically active protein is secreted in the apoplast (von Schaewen et al., 1990; Dickinson et al., 1991). We chose the transmembrane domain of yeast calnexin because we assumed that th ...
Specific Isotopic Labeling of Methyl Groups has Extended the
... sensitive, and were acquired using a room temperature probe at 800 MHz in 59, 58, and 21 hours26, respectively, with a 0.9 mM protein sample. The use of these experiments enabled the 1H methyl (1Hme) and 13C methyl (13Cme) resonances of nearly 78% of the ILV methyl groups in a 82 kDa protein (MSG) t ...
... sensitive, and were acquired using a room temperature probe at 800 MHz in 59, 58, and 21 hours26, respectively, with a 0.9 mM protein sample. The use of these experiments enabled the 1H methyl (1Hme) and 13C methyl (13Cme) resonances of nearly 78% of the ILV methyl groups in a 82 kDa protein (MSG) t ...
Evolutionary Gain of Function for the ER Membrane
... by either protein staining (A) or Western blotting plus immunodetection with anti-penta-histidine antibodies (B). Twenty-five percent of the input of the two Sec62 derivatives were run on the stained gel for comparison (A, lane 10). (C) GST-Sec63C hybrid was immobilized. Buffer, Sec62N (1 M), or Se ...
... by either protein staining (A) or Western blotting plus immunodetection with anti-penta-histidine antibodies (B). Twenty-five percent of the input of the two Sec62 derivatives were run on the stained gel for comparison (A, lane 10). (C) GST-Sec63C hybrid was immobilized. Buffer, Sec62N (1 M), or Se ...
Communication - Department of Molecular Physiology and Biophysics
... which lack dystrophin. Notably, α-syntrophin remains enriched at the neuromuscular junction (Fig. 4b) where utrophin is located (25). This further suggests that α-syntrophin associates with both dystrophin and utrophin in vivo. Thus far, our results indicate that syntrophin isoforms bind to each oth ...
... which lack dystrophin. Notably, α-syntrophin remains enriched at the neuromuscular junction (Fig. 4b) where utrophin is located (25). This further suggests that α-syntrophin associates with both dystrophin and utrophin in vivo. Thus far, our results indicate that syntrophin isoforms bind to each oth ...
In vivo characterization of the properties of SUMO1
... incubated at 4 ◦ C for 2–4 h. The beads were then washed three times with lysis buffer and all bound proteins were eluted by boiling the beads in NuPAGE LDS Sample buffer (4×) (Life Technologies). The eluates were analysed by Western blotting and Coomassie Blue staining. For IPs (immunoprecipitation ...
... incubated at 4 ◦ C for 2–4 h. The beads were then washed three times with lysis buffer and all bound proteins were eluted by boiling the beads in NuPAGE LDS Sample buffer (4×) (Life Technologies). The eluates were analysed by Western blotting and Coomassie Blue staining. For IPs (immunoprecipitation ...
In this section of the tutorial you will
... different forms of a given protein in order to describe the various reactions in the pathway. Each of these forms is a protein object in PRO and will have a distinct ID (e.g. PRO ID for smad2 isoform 1 and a PRO ID for smad2 isoform 1 phosphorylated in a given residue). By creating a RACE-PRO entry, ...
... different forms of a given protein in order to describe the various reactions in the pathway. Each of these forms is a protein object in PRO and will have a distinct ID (e.g. PRO ID for smad2 isoform 1 and a PRO ID for smad2 isoform 1 phosphorylated in a given residue). By creating a RACE-PRO entry, ...
Protein structure prediction
... • fats, sugars, ATP, ADP, … Macromolecules: hydrogen bonds, ionic, hydrophobic, Waals 1-5 kcal/mol Stability and bindings determined by the number of weak bonds: 3D shape • 20% proteins (50-104 amino acids) • RNA (102-104 nucleotides AGCU) • DNA (102-106 nucleotides AGCT) ...
... • fats, sugars, ATP, ADP, … Macromolecules: hydrogen bonds, ionic, hydrophobic, Waals 1-5 kcal/mol Stability and bindings determined by the number of weak bonds: 3D shape • 20% proteins (50-104 amino acids) • RNA (102-104 nucleotides AGCU) • DNA (102-106 nucleotides AGCT) ...
The DsbA Signal Sequence Directs Efficient
... methionine concentration of 90 mM for 15 s. For identification of polypeptides with or without their signal peptides, standards were prepared by labeling for 5 min in the presence or absence of azide, which allowed full incorporation of [35S]methionine. Labeling was terminated by addition of trichlo ...
... methionine concentration of 90 mM for 15 s. For identification of polypeptides with or without their signal peptides, standards were prepared by labeling for 5 min in the presence or absence of azide, which allowed full incorporation of [35S]methionine. Labeling was terminated by addition of trichlo ...
Ammonium Dodecyl Sulfate as an Alternative to Sodium Dodecyl
... from cells.5,6 Most biopolymer samples such as cell lysates contain a lot of salts that can be the potential source of sodium ions in subsequent MALDI analysis. However, prior to the use of SDS for protein extraction, the water-soluble proteins are commonly first extracted by using a buffer system t ...
... from cells.5,6 Most biopolymer samples such as cell lysates contain a lot of salts that can be the potential source of sodium ions in subsequent MALDI analysis. However, prior to the use of SDS for protein extraction, the water-soluble proteins are commonly first extracted by using a buffer system t ...
Characterization of Lipid Rafts from Medicago
... were shown to have certain properties of mammalian and yeast lipid rafts. A protein and lipid characterization was also performed from Arabidopsis (Arabidopsis thaliana) callus microsomes (Borner et al., 2005). Other studies have identified proteins present in DIMs of Arabidopsis leaves and the toba ...
... were shown to have certain properties of mammalian and yeast lipid rafts. A protein and lipid characterization was also performed from Arabidopsis (Arabidopsis thaliana) callus microsomes (Borner et al., 2005). Other studies have identified proteins present in DIMs of Arabidopsis leaves and the toba ...
Chemistry in living systems
... chemoselective drug assembly in the presence of living cells53, 54, 55. They reported that decanal and octyl aminoguanidine—both independently harmless to cells—react selectively to form a hydrazone-linked detergent capable of lysing cultured erythrocytes. This same strategy was used to generate inh ...
... chemoselective drug assembly in the presence of living cells53, 54, 55. They reported that decanal and octyl aminoguanidine—both independently harmless to cells—react selectively to form a hydrazone-linked detergent capable of lysing cultured erythrocytes. This same strategy was used to generate inh ...
Delivering of Proteins to the Plant Vacuole—An Update
... to the vacuole, where the fusion of the PVC/MVB with the tonoplast occurs [15]. As the trafficking to the vacuole can occur by different routes and may involve different intermediates it will be approached in more detail in the next section. In a simplified version of protein transport to the vacuol ...
... to the vacuole, where the fusion of the PVC/MVB with the tonoplast occurs [15]. As the trafficking to the vacuole can occur by different routes and may involve different intermediates it will be approached in more detail in the next section. In a simplified version of protein transport to the vacuol ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.